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Citations for Millipore Sigma :
3501 - 3550 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and the protein concentration was determined by bicinchoninic acid (BCA) assay (Merck Millipore) and by UV-visible spectroscopy using an ε of 24 mM⁻¹·cm⁻¹ at 280 nm ...
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bioRxiv - Immunology 2023Quote: ... The protein fractions with high purity were concentrated with a centrifugal concentrator (Millipore). TAB1 (468-504 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was transferred to an Immobilon®-FL PVDF Membrane (Millipore; IPFL00010) using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were concentrated with ultrafiltration centrifugal devices of 50-kDa cutoff (Amicon, Millipore). Monodispersed fractions of proteins were collected from size-exclusion columns (Superdex 200 10/300 GL ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM MgCl2 using a protein concentrator with a 10-kDa cutoff (Millipore, UFC801024 before loading onto a HiTrap Q HP column connected to an ÄKTA FPLC ...
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bioRxiv - Bioengineering 2022Quote: ... Tissue punches were suspended in protein extraction RIPA lysis buffer (Sigma-Aldrich Corp) containing protease and phosphatase inhibitors cocktail (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated using a 50 kDa cut-off Amicon device (Millipore). The concentrated protein was centrifuged at 14,000 xg for 15 mins before injecting onto a Superdex 200 (10/300 ...
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bioRxiv - Biochemistry 2023Quote: ... Then the protein was concentrated with centrifugal concentrators (UFC901096, Merck Millipore, Cork, Ireland), and loaded onto a SuperoseTM 6 Increase 10/300 GL gel-filtration column (29- 0915-96 ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were subsequently incubated for 2 h with Protein-G–Sepharose (Sigma-Aldrich). After 3 washes with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was transferred by electrophoresis to an Immobilon-P PVDF membrane (Millipore Corp.).
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bioRxiv - Cell Biology 2022Quote: ... the isolated proteins were eluted in 25 µL 2x Laemmli buffer (Sigma Aldrich) by boiling for 5 min at 95 °C and the supernatant prepared for mass spectrometry measurement.
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bioRxiv - Biophysics 2023Quote: ... Dialyzed protein was concentrated using an Amicon Ultra concentrator (30 kDa MWCO, Millipore) and aliquoted into 100 μl amounts and stored at −80 °C.
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-Golgi 58K protein (formimidoyltransferase-cyclodeaminase) mAb (G2404) was from Sigma-Aldrich and used at a dilution of 1:50∼100 ...
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bioRxiv - Cell Biology 2023Quote: Proteins separated with SDS-PAGE were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). Membranes were blocked with 5% skim milk in Tris-buffered saline with 0.05% Tween 20 for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... and rabbit anti-glial-fibrillary acidic protein (GFAP) (Millipore Sigma, AB5804, 1:500) on shaker ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were transferred onto nitrocellulose membranes (0.45 µm pore size; Sigma, cat. # GE10600012) for 2.5 h at 400 mA constant ...
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bioRxiv - Neuroscience 2022Quote: ... protein was further concentrated using Amicon Ultra-0.5 mL centrifugal filters (Millipore Sigma) prior to use ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated with first and secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Protein gels were transferred for 60 min to 0.2µm PVDF membranes (ISEQ00010, Sigma) using the semi-dry Trans-Blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... and protein bands were visualised via coating the membrane in tetramethylbenzidine (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... and precipitated proteins competitively eluted with Flag peptide (150 ng/µl; F4799, Sigma). Lysates were denatured in SDS-PAGE buffer (4x Lämmli buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were concentrated and the protein concentration measured by BCA assay kit (Sigma). ATPase assay ...
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bioRxiv - Immunology 2023Quote: ... OVOL1 protein expression were determined by rabbit anti-human OVOL1 antibody (Sigma Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred onto PVDF membranes (Immobilon-FL, 0.45 μm pore size, Millipore) following standard procedures ...
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bioRxiv - Developmental Biology 2023Quote: ... for 1 hr at 115 V before transferring to protein membrane (IPV00010, Millipore) at 50 V for 2 hr ...
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bioRxiv - Developmental Biology 2023Quote: Protein was extracted from maternal liver using RIPA buffer (Sigma-Aldrich Corp., R0278). Protein concentration was determined by a Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). After ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantifications were normalized with the β-actin signal (A3854; Sigma-Aldrich®).
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bioRxiv - Neuroscience 2023Quote: ... followed by rotating incubation with 20 µl of protein G-Agarose beads (Sigma) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE and then transferred to nitrocellulose membranes (Millipore). Membranes were probed with a 1:1000 dilution of anti-SARS-CoV-2 S mouse monoclonal primary antibody (1A9 ...
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bioRxiv - Biochemistry 2023Quote: ... and aldehyde concertation was determined using Protein Carbonyl Content Assay Kit (Sigma-Aldrich). Aldehyde concentration was determined to be 4 mol aldehyde per mol protein ...
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bioRxiv - Molecular Biology 2023Quote: ... to which Magna ChIP™ Protein A+G Magnetic Beads (Millipore, 16-663) were added for 2hour at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins separated by SDS-PAGE were transferred to Immobilon-P membranes (Merck Millipore). Three major bands on the membrane were cut and subjected to N-terminal Edman sequencing by Hokkaido System Science Co. ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was determined using a bicinchoninic acid kit (BCA, Sigma, BCA1-1KT) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
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bioRxiv - Molecular Biology 2023Quote: ... Fractions containing protein were concentrated in 50-kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were flash frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Molecular Biology 2023Quote: ... fractions containing protein were concentrated in 10-kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were flash frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was concentrated in a 50 kDa cut-off Vivaspin (Millipore) and run on a Superose 6 size exclusion column (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 µM Rho-associated protein kinase (ROCK) inhibitor (Merck Millipore, SCM-075). On day 3 ...
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bioRxiv - Biophysics 2023Quote: ... the protein was digested online by a pepsin/FPXIII (Sigma-Aldrich P6887/P2143) mixed protease column maintained at 20 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein samples were then concentrated using Amicon Ultra centrifugal filters (30k MWCO, Millipore). The protein concentration of eluates were estimated with a Bradford assay (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... proteins from the gel were transferred onto a PVDF membrane (Millipore, cat#IPVH00010). The membrane was subsequently blocked and incubated with primary and secondary antibodies ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Cancer Biology 2023Quote: ... the protein was concentrated using an 10K MWCO centrifugal concentrator (Amicon-15, Millipore) to 20 mg/mL and purified by size exclusion chromatography on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Proteins were then precipitated upon addition of 50% trichloroacetic acid (TCA, Sigma-Aldrich) to a final concentration of 5% ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins coupled to streptavidin-coated magnetic beads were reduced with dithiothreitol (Sigma-Aldrich) and alkylated with iodoacetamide ...
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bioRxiv - Physiology 2023Quote: ... Proteins were then concentrated to approximately 3 mg/ml by ultrafiltration (Millipore, UFC905096) at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... purified WT or ΔFN2 proteins were incubated with rat tail collagen (EMD Millipore) and then co-IP was carried out as described above.
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bioRxiv - Plant Biology 2023Quote: ... The protein eluent was concentrated using a 100-kDa cut-off Centricon (Millipore) and subsequently loaded onto a Superose 6 Increase 5/150 GL column (Cytiva ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...