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Citations for Millipore Sigma :
3651 - 3700 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred from SDS-PAGE gels to PVDF-membranes (Immobilon-P, Millipore 0.45µm) in transfer buffer (per 1 l ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant containing cleaved proteins were concentrated using a 50K MWCO concentrator (EMD Millipore) to 1 mL ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant containing cleaved proteins was concentrated using a 100K MWCO concentrator (EMD Millipore) to 500 µL and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Neuroscience 2019Quote: ... Protein synthesis was inhibited by including 20 μg/mL cycloheximide (Sigma-Aldrich, Cat #C4859) in the imaging medium ...
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bioRxiv - Biophysics 2019Quote: ... 15 μL diluted proteins were mixed with 1 μL 100x Sypro Orange (Sigma-Aldrich) and loaded in LightCycler 480 Multiwell Plate 96 (Roche ...
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bioRxiv - Plant Biology 2019Quote: ... Purified proteins were then concentrated using Amicon Ultra concentrators (molecular-weight cutoff 10,000; Millipore) and purity and structural integrity was assessed by SDS-PAGE and Right Angle Light Scattering (RALS) ...
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bioRxiv - Cancer Biology 2020Quote: ... and antibody/bead complexes isolated with Magna ChIPTM Protein A+G magnetic beads (Millipore). Complexes were washed ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred onto a PVDF membrane (0.45 μm pore size, Immobilon-P, Millipore) using semidry transfer (Biorad) ...
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bioRxiv - Plant Biology 2020Quote: ... total proteins were incubated with 50 µL agarose conjugated with anti-Myc antibody (Sigma) for 2 h ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein content was determined using the Bradford reagent (Serva) with BSA (Sigma-Aldrich) as a standard (Bradford ...
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bioRxiv - Cell Biology 2019Quote: ... total protein content was stained using 0.1% (w/v) Ponceau S (Sigma-Aldrich, P3504) in 1% (v/v ...
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bioRxiv - Biochemistry 2019Quote: ... The delipidated NPC2 proteins were incubated with increasing concentrations of cholesterol (>99%) (Sigma Aldrich), in DMSO ...
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bioRxiv - Microbiology 2019Quote: ... and the soluble proteins were separated from the OMVs using an iodixanol (Optiprep, Sigma) density gradient ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were resolved by 10% SDS-PAGE and electrotransferred onto polyvinylidene fluoride membrane (Millipore) in transfer buffer (10 mM CAPS ...
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were then concentrated on either Amicon (Merck Millipore, MWCO 10 kDa) or Vivaspin (Sartorius ...
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bioRxiv - Bioengineering 2020Quote: ... using one of the following extracellular matrix (ECM) protein products: laminin (Laminin 111, Sigma L2020 ...
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bioRxiv - Pathology 2021Quote: ... proteins were transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Systems Biology 2020Quote: ... Total protein concentration was measured using bicinchoninic acid assay (BCA Kit, Sigma-Aldrich, USA).
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using Centriprep centrifugal filter devices (Millipore; molecular weight cutoff, 10 kDa) and further purified using size exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reduced using 2.5 μL of dithiothreitol (200 mM DTT stock solution) (Sigma) at 90 °C for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... The immune complexes were incubated with protein A Sepharose beads (Sigma, St. Louis, MS) at 4°C for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... Protein purity was assessed by 20% SDS-PAGE stained with Coomassie Brilliant Blue (Sigma). PageRule Unstained Broad Range Protein Ladder and PageRuler Plus Prestained Protein Ladder (both Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and Flag-tagged FtsX proteins were detected by using ANTI-FLAG® antibody (Sigma) as previously described by Stamsås et al ...
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bioRxiv - Genetics 2021Quote: ... The immune-reactive proteins were detected using the Luminata Crescendo Western HRP substrate (Millipore).
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bioRxiv - Biochemistry 2020Quote: ... Bound proteins were eluted by 1X PBS with 10 mM reduced glutathione (Sigma Aldrich). Fractions containing VCA-NWASP were concentrated and dialyzed to 20 mM Tris (pH 7.4) ...
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bioRxiv - Microbiology 2021Quote: ... Target proteins were bound to 1 mL Ni-NTA His-Bind resin (Millipore Sigma) in gravity columns ...
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bioRxiv - Biochemistry 2020Quote: Antibiotics used in protein expression and other analytical grade chemicals were purchased from Sigma. Unfractionated heparin was purchased from Sigma as a sodium salt ...
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bioRxiv - Biochemistry 2020Quote: ... FphF protein was initially purified by Ni2+ affinity chromatography (HIS-Select resin, Sigma-Aldrich) using an elution buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... were transiently transfected with the S protein expression construct using polyethylenimine (PEI; Sigma-Aldrich) in FreeStyleMedia (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... Mass inaccuracies were corrected using ProteoMass Peptide and Protein MALDI-MS calibration standard (Sigma). Glycan compositions were manually inspected using MALDI LIFT-TOF/TOF MS/MS.
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bioRxiv - Biochemistry 2020Quote: ... albumin (66 kDa) and carbonic anhydrase (29 kDa) protein molecular weights standards (Sigma Aldrich). Corresponding fractions for each chromatogram between 12 and 18 ml with an interval of 0.5ml were analyzed by SDS-PAGE (Biorad ...
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bioRxiv - Biochemistry 2020Quote: ... Protein visualization was achieved by using Coomassie Brilliant Blue G250 staining (Sigma-Aldrich, Germany) and imaging with a ChemDoc MP Imaging System (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... The proteins were concentrated using Amicon centrifugal filters (5k MWCO or 10k MWCO, Millipore) and purified further by size exclusion chromatography (Superdex 75 Increase ...
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bioRxiv - Neuroscience 2021Quote: ... and those directed against the following proteins: Actin (rabbit, Sigma, A2066, WB 1 : 2000), GFAP (mouse ...
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bioRxiv - Cell Biology 2021Quote: Protein was isolated from cells using 1X RIPA lysis buffer (EMD Millipore, Burlington, MA) containing 1X protease inhibitor cocktail (Thermo-Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were digested with lysyl endopeptidase (Wako Pure Chemical Industries) and trypsin (Sigma-Aldrich) with an enzyme:substrate ratio of 1:75 at 37 °C for 16 h ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing cleaved proteins was concentrated using a 100K MWCO concentrator (EMD Millipore) to 500 µL and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Cell Biology 2021Quote: ... were coated with 5 µg/mL human plasma fibronectin purified protein (EMD Millipore Corporation). Cells were seeded at a density of 315,000 cells per insert in 250 µL HITES media ...
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bioRxiv - Cancer Biology 2020Quote: Protein was extracted from tissue or cells by addition of RIPA Buffer (Sigma Aldrich) including complete Protease Inhibitor Cocktail Tablets (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein lysates were run on SDS-PAGE gels and transferred to PVDF membranes (Millipore). Membranes were blocked and probed with antibodies directed against RAS (RRID:AB_397425 ...
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bioRxiv - Cell Biology 2021Quote: ... the protein sample is transferred to the polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, USA). Blocking the membrane with 5% BSA (Bovine serum albumin) ...
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bioRxiv - Pathology 2021Quote: ... Immunoprecipitated proteins were separated on acrylamide-SDS gels and transferred to PVDF membranes (Millipore). The membranes were probed with rabbit anti SLP2 and mouse anti Flag M2 ...
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bioRxiv - Plant Biology 2021Quote: ... an equal amount of total protein extracts was incubated with anti-GFP (Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The pellet was dried and resuspended in Protein Extraction Reagent (C0856, Sigma-Aldrich®) supplemented with 1% Protease inhibitor (P9599 ...
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bioRxiv - Cell Biology 2021Quote: ... was conjugated with Atto-647N using a protein labeling kit (cat # 76508 Sigma-Aldrich). PDMS stamps were incubated with solution containing 50μg/ml fibronectin and 1μg/ml of the conjugated fibronectin in Dulbecco’s phosphate-buffered saline (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... and the fractionated proteins detected by direct staining of the gel with InstantBlue (Sigma) or by western blot ...