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Citations for Millipore Sigma :
3601 - 3650 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Then pre-processed protein A agarose (Catalog No. P3476, Sigma, MO, USA) was added to mixture ...
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bioRxiv - Biophysics 2021Quote: ... Protein expression was performed in Origami™ 2 (DE3) competent cells (Novagen) using LB medium ...
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bioRxiv - Biophysics 2021Quote: ... Crosslinked proteins were precipitated with 20% (w/v) trichloroacetic acid (TCA, Sigma) on ice for 90 minutes ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were visualized by gel staining with Coomassie Brilliant Blue (Sigma Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked with 5% skim milk in TBST (10 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was filtered with a 0.22 μm pore size filter (Millipore) prior to size-exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were detected with Luminata Forte Western HRP Substrate (EMD Millipore) or SuperSignal™ West Atto Ultimate Sensitivity Substrate (A38555 ...
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bioRxiv - Cell Biology 2022Quote: ... and then the proteins were transferred onto polyvinylidene difluoride membranes (Millipore, IPVH00010). Membranes were then probed with anti-α-SMA (Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... and it was determined by BCA protein assay (Sigma, Sao Paulo, Brazil). A total of 10 µg protein concentrate was used in a 10% or 12% polyacrylamide gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated with ultra-filter 0.5 (Millipore, Sao Paulo, Brazil), and it was determined by BCA protein assay (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... protein expression was induced using 1 μg/mL of doxycycline (Sigma, D9891) for 16 h.
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bioRxiv - Biochemistry 2022Quote: ... 50µL of a suspension of Protein G coupled to agarose beads (Sigma) previously equilibrated in Bax buffer (20mM Tris/HCl pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... 500μg of protein was incubated on magnetic beads (FLAG M2 beads (Sigma), GFP beads (Chromotek ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were transferred to Immobilon-P PVDF membrane (Merck Millipore, MA, U.S.A). The membranes were incubated for 1h using 5% low-fat milk solution in 1X TBS (50 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2022Quote: ... Total protein was assayed by incubation with Ponceau staining (Sigma-Aldrich P7170), which was later destained and imaged on a Sapphire biomolecular imager (Azure Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins entrapped in gel bands were reduced with 10 mM dithiothreitol (Sigma) at 60°C for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... The proteins were wet-transferred to a PVDF membrane (Immobilon-P; Millipore) for 2.5 hours at 100 V ...
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bioRxiv - Neuroscience 2022Quote: ... The final measurements were normalized to protein content using sulforhodamine B (Sigma) (Johnson et al. ...
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bioRxiv - Bioengineering 2022Quote: Biotinylated proteins were obtained using Biotin N-hydroxysuccinimide ester reagent (Sigma Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... and associated proteins were eluted in 125 μg/ml 3xFLAG peptide (Sigma) for 30 minutes on ice.
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bioRxiv - Biophysics 2022Quote: ... and ASH2L proteins were individually expressed in Escherichia coli (Rosetta II, Novagen) and purified as described previously (66) ...
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bioRxiv - Biochemistry 2022Quote: ... His6-tagged proteins were purified with Ni-NTA His-Bind Superflow (Novagen) and dialyzed against PBS using a Pur-A-Lyzer™ Midi Dialysis Kit (Merck).
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bioRxiv - Cancer Biology 2022Quote: ... Protein G Sepharose 4 Fast Flow Beads (Millipore Sigma, GE17-0618-01) were added and incubated for 1 h at 4°C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved by SDS-PAGE and transferred to PVDF membrane (Millipore). Membranes were incubated overnight with primary antibodies (Antibody list shown in Table 3) ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were then concentrated by ultrafiltration using Amicon filters (EMD Millipore).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 300 μg proteins were incubated with 20 mM dithiothreitol (DTT; Sigma Aldrich) for 10 min at 95°C on a thermomixer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Protein lysates were concentrated using Microcon-10kDa Centrifugal Filter Units (Merck Millipore), according to manufacturer’s instructions and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... and immunoprecipitated using 50 μl of protein G agarose bead slurry (Millipore). Monoclonal antibodies recognizing CD2v-HA were generated by immunizing BALB/c mice with immunoprecipitated CD2v-HA (100 μg/dose) ...
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bioRxiv - Genetics 2021Quote: ... Transfer was confirmed by visualising protein bands with 0.1% Ponceau S (Sigma) in 5% acetic acid (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were transferred to Immobilon-P Polyvinylidene fluoride (PVDF) membranes (Millipore) in Tris-Glycine-SDS buffer containing 20% methanol for 2h at 50V ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins of interest were revealed by Clarity Western Blot ECL substrate (Millipore).
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bioRxiv - Neuroscience 2021Quote: ... and protein was eluted with 100 μg/mL FLAG peptide (Sigma-Aldrich) in elution buffer for 1 hour at 4°C with rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... The chromatin solution was pre-cleared with protein A sepharose (Sigma-Aldrich), 10% retained (input ...
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bioRxiv - Immunology 2020Quote: ... Separated proteins were transferred to PVDF Immobilon-FL membranes (Millipore, Billerica, MA) using the BioRad Trans-Blot Turbo Transfer System (Hercules ...
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bioRxiv - Neuroscience 2021Quote: ... immunoreactive proteins were visualized using Immobilon® Classico Western HRP Substrate (Millipore) or Immobilon® Forte Western HRP Substrate (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Protein was reduced with tris (2-carboxyethyl) phosphine (Sigma-Aldrich, MO, USA) and alkylated with methylmethanethiosulphonate (MMTS ...
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bioRxiv - Physiology 2021Quote: ... All images were normalized to Amido Black total protein stain (A8181, Sigma) using the entire lane corresponding to each sample.
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bioRxiv - Biochemistry 2021Quote: ... Protein solution (4 mg/mL in 50 mM Tris-HCl (Sigma-Aldrich), pH 8.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich) and purified with a Superdex 200 pg (Cytiva ...
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bioRxiv - Neuroscience 2020Quote: ... the protein samples were reduced with 20 mmol/L dithiothreitol (DTT, Sigma) at 37 °C for 1 h and alkylated with 50 mmol/L iodoacetamide (IAA ...
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bioRxiv - Microbiology 2021Quote: ... or thrombin protease (10 U/mg of fusion protein, Sigma-Aldrich, Germany). Importin β1 ...
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bioRxiv - Microbiology 2021Quote: Immunoprecipitation was conducted using PureProteomeTM Protein A/G Mix Magnetic Beads (Millipore). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to polyvinyl difluoride membranes (Immobilon-P, #IPVH00010, Millipore/Sigma) and incubated overnight at 4 °C with the primary anti-Plin2 antibody (ADFP rabbit polyclonal antibody ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to polyvinyl difluoride membranes (Immobilon-P, #IPVH00010, Millipore/Sigma) and incubated overnight at 4 °C with the primary anti-Plin2 antibody (ADFP rabbit polyclonal antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... Subsequently protein thiol groups were blocked with 10mM iodoacetamide (Sigma-Aldrich, USA) at RT for 45 min ...
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bioRxiv - Genomics 2022Quote: Fusion protein overexpression was induced by treatment with doxycycline (dox) (Sigma Aldrich) at a concentration of 1µg/mL for the specified amount of time ...
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bioRxiv - Biochemistry 2022Quote: ... All reagents used for protein purification and enzymatic assays were from Sigma.
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bioRxiv - Neuroscience 2022Quote: ... protein content was additionally measured using the Bradford assay (Sigma-Aldrich, USA). Bovine serum albumin dissolved in 1xPBS was used for the standard curve ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using His•Bind® Purification Kit (Novagen) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were probed with rabbit anti-HA (1:1,000 Sigma-Aldrich, H3663) or anti-β-actin as loading control (1:10,000 Sigma-Aldrich ...