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Citations for Millipore Sigma :
3851 - 3900 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... total protein was visualized using PonceauS staining (Sigma-Aldrich, St. Louis, MO), blocked using 5% non-fat milk powder in tris-buffered saline with tween (TBST ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein was then concentrated using 3K-50K MWCO Amicon concentrators (Millipore). All proteins were aliquoted in PCR tubes ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein was then concentrated using 3K-50K MWCO Amicon concentrators (Millipore). All proteins were aliquoted in PCR tubes ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-glial fibrillary acidic protein (1:1000, Millipore Sigma, #MAB3402), rat monoclonal anti-CD45 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... 50 µg protein per sample were reduced with 10 mM DTT (Sigma) for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... the proteins were transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, USA), which was then blocked with 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated with 1:4 trichloroacetic acid solution (TCA, Sigma Aldrich) and washed with acetone ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg protein per sample was reduced with 3 mM TCEP (Sigma) and alkylated with 10 mM iodoacetamide (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... and the proteins transferred onto polyvinylidene fluoride membrane (Immobilion-P, Millipore, USA) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and 4 g digitonin:1 g mitochondrial protein (Sigma-Aldrich, #D141-100MG). Mitochondria were incubated on ice for 20 minutes and then centrifuged at 20,000 x g for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... total protein was detected with Amido Black (Sigma-Aldrich, St. Louis, MO). Chemiluminescence was measured in a ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Purified protein was then concentrated to 40mg/mL by centrifugal concentration (Millipore).
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected by immunoblotting using anti-Glutathione-S-Transferase (Sigma-Aldrich), anti-polyHistidine-Peroxidase (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... protease incubation (Pepsin Porcine, 1:50, enzyme to protein ratio, Sigma-Aldrich) was performed for 4 h at 37 °C in 40 mM HCl in a total volume of 2 ml (pH 2) ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were stained using ReadyBlue protein gel stain (Sigma-Aldrich, RSB-1L) followed by de-staining with water ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was concentrated using Amicon Ultra-4 10 kDa MWCO (Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... They were incubated with recombinant ULK1 proteins (Sigma, #SRP5096-10UG, Darmstadt, Germany) in the kinase buffer (20 mM HEPES (pH 7.5) ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2 h on ice before mixing with protein G-Sepharose (Millipore). The antibody complex was washed three times in lysis buffer and once in Tris-buffered saline (TBS) ...
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bioRxiv - Immunology 2023Quote: Dry protein pellets were dissolved in 100mM TrisHCL (pH 8.5, Sigma T1503) to determine the protein concentration using the Bradford protein assay (Thermo Scientific 23200) ...
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bioRxiv - Molecular Biology 2023Quote: ... protein synthesis was inhibited by blocking translation step using cycloheximide (Sigma, #C7698) and MMP activity was inhibited by ilomastat (GM6001) ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein was extracted in RIPA buffer containing protease inhibitor mixture (Sigma) and phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Transferred proteins were stained in Ponceau S (0.1% Ponceau S (Sigma-Aldrich), 5% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-glial fibrillary acidic protein (GFAP, 1:1000, Millipore Sigma, USA), anti-NeuN (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... Following a reduction of proteins with 10 mM DTT (Sigma-Aldrich, Germany) in 100 mM TEAB at 57 °C for 30 min and dehydration of gel pieces ...
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bioRxiv - Immunology 2023Quote: ... The protein in PBS was 0.22um filtered using spin filters (Millipore Sigma) prior to Size exclusion Chromatography (SEC) ...
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bioRxiv - Microbiology 2023Quote: ... The elution containing native DnaN protein was concentrated by ultrafiltration (Millipore, UFC901096) and maintained in 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: Cells for protein extraction were lysed using RIPA lyses buffer (Sigma #R0278) containing protease and phosphatase inhibitor cocktails (cOmplete™ ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010 ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were stained with ReadyBlue Protein Gel Stain from Sigma-Aldrich (USA) and imaged and analyzed on a ChemiDoc Imaging System by Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were then transferred to 0.45 µm PVDF membranes (Immobilon-P, Millipore) in a wet tank apparatus ...
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bioRxiv - Cell Biology 2023Quote: ... and protein analysis of tau and β-III-tubulin (Sigma T-8660) by western blot as described above.
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bioRxiv - Genomics 2023Quote: ... Proteins were visualised using Immobilon Forte HRP substrate (Merck Millipore, MA, USA) and a Fusion FX Chemi Imager (Vilber Lourmat ...
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bioRxiv - Cell Biology 2023Quote: Total protein was extracted from cell lines using TRI Reagent (Sigma, UK). 40μg of isolated protein samples were then separated using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Physiology 2023Quote: ... Final protein concentrations were calculated by Bradford assay (Sigma-Aldrich Canada, Ltd.,) according to manufacturer’s guidelines with bovine serum albumin (BioRad Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µl Magna ChIP™ Protein A Magnetic Beads (Sigma, 16-661) and antibodies (1 µg per 1 million-cell chromatin ...
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bioRxiv - Cell Biology 2023Quote: Equal amounts of protein samples were supplemented with β-mercaptoethanol (Sigma; M6250) and diluted in 1X Laemmli Sample buffer (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Eluted protein was concentrated using spin concentrators (Millipore Amicon Ultra-15 -3K) to ∼1.5-2 mL and subsequently dialyzed in one liter of storage buffer (150 mM NaCl ...
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bioRxiv - Genetics 2023Quote: ... Protein lysate was prepared in Radio-immunoprecipitation assay (RIPA) buffer (Merck Millipore) with a protease inhibitor cocktail (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). The membranes were blocked with 5 % skimmed milk in TTBS buffer (10 mM Tris-HCl ...
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bioRxiv - Plant Biology 2024Quote: ... MBP fusion proteins were expressed in Escherichia coli Rosetta (DE3) pLysS (Novagen) and purified using Amylose Resin (New England Biolabs ...
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bioRxiv - Biophysics 2024Quote: ... The digested protein samples were then concentrated with an Amicon Ultra (Millipore) concentrator (50 kDa MWCO ...
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bioRxiv - Cancer Biology 2024Quote: ... the protein was transferred to 0.45μm PVDF membranes (Millipore Sigma, Cat#IPVH00010) with transfer buffer (formula ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto a 0.45-μm polyvinylidene difluoride membrane (Millipore). Next ...
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bioRxiv - Cell Biology 2024Quote: Cellular protein was collected in RIPA buffer (R0278, Millipore Sigma, Burlington, MA) containing ReadyShield® Protease and Phosphatase Inhibitor Cocktail (PPC- 2020 ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... 50 µL of agarose beads coupled to protein A (Millipore 16–157) or G (Millipore 16–201 ...
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bioRxiv - Immunology 2024Quote: ... were coupled to 30 μl Protein G Sepharose™ beads (#P3296, Millipore) by 2 h incubation at 4°C with rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon-P, Millipore, IPVH100010). Membranes were blocked for at least 1 h with 5% non-fat dry milk and 3% BSA in TBS-T (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein-containing factions were concentrated with Amicon Ultra Filter Devices (Millipore, USA) 10 kDa cutoff ...
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bioRxiv - Biochemistry 2024Quote: ... The separated proteins were then transferred to a PVDF membrane (Millipore, IPVH00010) and immunodetection was carried out using antibodies against the target proteins ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were detected using anti-FLAG (Sigma-Aldrich, ST. Louis, MO, USA) and HRP-labeled anti-mouse IgG (Cytiva ...