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Citations for Millipore Sigma :
3501 - 3550 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Samples were incubated with primary antibodies (mouse anti-acetyl-alpha tubulin antibody, clone 6-11B-1 [Millipore-Sigma Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... The primary antibodies used in this study were a mouse anti-acetylated tubulin antibody (Sigma-Aldrich #T7451) diluted at 1:200 and a goat anti-serotonin antibody (Immunostar #20080 ...
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bioRxiv - Molecular Biology 2023Quote: ... using anti-FLAG antibody for CD44 (1:1000 dilution of anti-Flag M2-HRP antibody; Sigma, A8592) and anti-GST antibody for GST-MSN (1:1000 dilution of anti-GST (2.6H1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were mouse anti-collagen type I monoclonal antibody (Sigma-Aldrich, #C 2456, diluted 1:100), mouse anti-collagen type II monoclonal antibody (Developmental Studies Hybridoma Bank ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used as primary antibodies: mouse anti-vinculin (1:400; clone hVIN-1, Sigma), rabbit anti-non-muscle myosin heavy chain II-A (NMIIA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A sequential pull down with homemade DMC1 antibody followed by commercial H3K4me3 antibody (Millipore (cat#07-473)) was carried out with the same tissue lysate ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used in this study included rabbit anti-human monoclonal antibodies of ONECUT2 (Sigma HPA057058) at 1:50 ...
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bioRxiv - Cell Biology 2024Quote: ... A rabbit polyclonal anti-luciferase antibody and a goat anti-rabbit secondary antibody were obtained from Millipore-Sigma.
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bioRxiv - Evolutionary Biology 2024Quote: ... The cells were reacted with rabbit anti-HA antibody and mouse anti-FLAG antibody (#F3165, Sigma-Aldrich) diluted with antibody reaction buffer [PBS ...
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bioRxiv - Neuroscience 2024Quote: ... antibodies were stripped using a 20min incubation in stripping solution (ReBlot Plus Mild Antibody Stripping Solution, Millipore).
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bioRxiv - Molecular Biology 2024Quote: The antibodies used for immunoblot analysis were: Mouse monoclonal antibody to FLAG peptide (Sigma #F1804, 1:5000), Mouse monoclonal antibody to β-actin (Cell Signaling #4967 ...
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bioRxiv - Immunology 2024Quote: ... Bound primary antibodies were detected with horseradish peroxidase (HRP)-conjugated secondary antibodies against the different species (Sigma). Proteins were detected by chemiluminescence (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: Hashtag barcoding antibody-oligos (HTOs) were conjugated to the mouse anti-dpERK monoclonal antibody (Sigma-Aldrich, M9692) using the CITE-seq hyper antibody-oligo conjugation protocol (https://cite-seq.com/) ...
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bioRxiv - Developmental Biology 2024Quote: ... The cleared lysates were incubated with Dynabead bound antibody complexes of either HA-specific antibody (Sigma H3663) or with normal mouse IgG overnight at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... proteins were transferred to PVDF membranes and blocked with 5% BSA (Sigma) followed by probing with mAb MoPn-40 (1:1,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... the proteins were transferred to an Immobilon P membrane (EMD Millipore, IPVH00010) at 25°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were eluted with 40 µl 3x FLAG peptide (Sigma-Aldrich) and collected by centrifugation for 30 min at 100xg.
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bioRxiv - Cancer Biology 2021Quote: ... separated protein bands were transferred into polyvinylidene fluoride membranes (Millipore, Cat. #IPVH00010). Then ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore) and the membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat dry milk for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was wet transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon P, Millipore) in Criterion blotter cells (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were concentrated by ultrafiltration using Amicon Ultra centrifugal filters (Merck Millipore) with 50 kDa cut off ...
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bioRxiv - Cell Biology 2020Quote: ... otherwise all other protein labelling were using 1% Triton X100 (Sigma-Aldrich) in PBS permeabilization for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... followed by the addition of 5 mg protein A beads (Sigma, P1406) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... 20µl of DBX-washed Magna ChIP Protein W+G magnetic beads (Millipore) were added to each sample and rotated for 1hr at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Protein concentration of tissue lysates was determined using bicinchoninic acid (Sigma-Aldrich). Proteins were denatured with gel loading buffer (50 mM Tris [pH 6.8] ...
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bioRxiv - Microbiology 2021Quote: ... Protein complexes were purified with α-FLAG M2 affinity gel (Sigma-Aldrich) as described for co-IP ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were extracted and purified using the Bugbuster reagent (Novagen, Inc). To raise antibodies against TRF4 or TIMRHOM1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were electroblotted to polyvinylidene fluoride membranes (IPFL00010, EMD Millipore, Billerica, MA), incubated with primary antibodies (see Supplemental Table 1 ...
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bioRxiv - Immunology 2020Quote: ... and 50 μl of horseradish peroxidase-Staphylococcal protein-A conjugate (Sigma-Aldrich) diluted 1:3000 was added ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were transferred to an Imobilon-PPVDF membrane (Millipore, Billerica, MA, IPVH00010). Membranes were probed with rabbit anti-chick RUNX2 primary antibody (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich) and then purified with Superdex 200 pg (GE Healthcare) ...
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bioRxiv - Pathology 2022Quote: ... Target proteins were detected using ECL western blot detection solution (Millipore, WBKLS0500). Primary antibodies used for immunoblotting were as follows ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore), which were subsequently blocked with 5% blotting grade blocker (cat# 170-6404 ...
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bioRxiv - Neuroscience 2021Quote: ... Guanine nucleotide-binding protein subunit alpha-11 (1:1,000; Millipore 05-855R), Guanine nucleotide-binding protein G(q ...
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bioRxiv - Neuroscience 2021Quote: ... we used the EZ-Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (Millipore) following the manual with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich), using BSA as a standard and aliquots maintained at −80°C until required ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-Ubiquitinylated proteins,clone FK2 (cat# 04-263, Merck Millipore; 1:1000). Ponceau solution was prepared with Ponceau BS (cat# B6008 ...
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bioRxiv - Molecular Biology 2021Quote: ... The separated proteins were transferred onto PVDF membrane (Millipore, Billerica, Massachusetts, USA) using phosphate based transfer buffer (10 mM sodium phosphate monobasic ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted protein was then precipitated using 100% trichloroacetic acid (Sigma-Aldrich) added drop wise to 20% of the final elution followed by vortexing and incubation at 4°C overnight prior to pelleting at 21130 x g for 20 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: p53 and E2C proteins were labeled with fluorescein isothiocyanate (FITC) (Sigma-Aldrich), adapting the manufacturer’s protocol to obtain sub-stochiometric labeling enough to visualize the samples by fluorescence and confocal microscopy ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein bands were reversibly stained with Fast Green FCF (Sigma, F7252) and imaged on ImageQuant LAS 4000 or Amersham ImageQuant 800 (GE Healthcare Life Sciences ...
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bioRxiv - Genomics 2020Quote: ... Protein loading was checked by staining membranes with Ponceau staining solution (Sigma) for 2min at room temperature ...
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Genomics 2020Quote: ... Cells were pelleted in Eppendorf® protein LoBind tubes (Sigma Aldrich Z666505) washed twice with ice cold PBS and snap-frozen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The separated proteins were then transferred to PVDF membranes (0.45μm, Millipore, USA), and block with 5% milk for 1h ...
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bioRxiv - Cell Biology 2020Quote: ... we use the PureProteome Protein G Magnetic Bead System (EMD Millipore Corp.). 1 mg protein extract was used for immunoprecipitation and incubated with protein G beads and 4 μg mouse anti-GFP antibody (B-2 #sc-9996 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein samples were treated with 10 mM dithiothreotol (DTT) (Sigma, MO) at 56 °C for 1 h and 55 mM IAA (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
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bioRxiv - Physiology 2020Quote: ... proteins were transferred to a PVDF membrane (Immobilon-P, Merck Millipore, IPVH00010) with 160 mA for 90 min ...
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bioRxiv - Genetics 2020Quote: ... Nuclear and cytoplasmic protein extracts were prepared using NXTRACT kit (Sigma-Aldrich) according manufacturer’s instructions.