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Citations for Millipore Sigma :
3451 - 3500 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... twice with LiCl wash buffer (10 mM Tris-HCl [pH 8], 250 mM LiCl, 0.5% sodium deoxycholate, 1 mM EDTA, 0.5% IGEPAL CA-630 [Sigma]), and twice with 10 mM Tris-HCl [pH 8] ...
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bioRxiv - Molecular Biology 2020Quote: ... Target cells were infected for 48 hr with lentiviral supernatants supplemented with 8 µg/ml polybrene (Sigma-Aldrich). Following a 24-hr recovery in fresh media ...
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bioRxiv - Microbiology 2020Quote: ... in THF (tetrahydrofurane) and 2 µL 200 mM ATPS (8-aminopyrene-1,3,6-trisulfonic acid trisodium salt, Sigma-Aldrich) in 15% acetic acid (v/v) ...
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bioRxiv - Physiology 2019Quote: Chemotaxis of hMSCs was assessed using Boyden chambers with a pore size of 8 μm (Merck Millipore, PIEP12R48). Cells were seeded on the upper membrane in serum free α-MEM medium at a density of 30,000 cells/cm2 and allowed to adhere 4 h before being transferred to the wells containing chemotactant (Medium (serum free) ...
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bioRxiv - Immunology 2019Quote: ... Viral particles were added to cultured THP-1 cells in R10 together with 8 μg/ml Polybrene (Sigma) to improve transfection efficiency ...
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bioRxiv - Microbiology 2019Quote: ... and forced through a 25-mm diameter Whatman Nuclepore Track-Etched Membrane with 8-µm holes (Sigma-Aldrich). The cellular debris ...
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bioRxiv - Neuroscience 2020Quote: Replicates of hiPSC-derived neurons were harvested with lysis buffer (8 M Urea, 50 mM ammonium bicarbonate (Sigma), EDTA-free protease inhibitor Cocktail (Roche) ...
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bioRxiv - Cell Biology 2019Quote: ... Concentrated lentivirus was diluted with growth medium and mixed 1:1 with polybrene (8 μg/ml; EMD Millipore) in Opti-MEM (Gibco) ...
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bioRxiv - Bioengineering 2021Quote: SMCs derived from hiPSCs were suspended (2.5×105 cells) in 60μl of an 8 mg/ml fibrinogen solution (Sigma). Hence ...
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bioRxiv - Biochemistry 2020Quote: ... we intraperitoneally (i.p.) administrated the AMPA/kainate receptor agonist kainate (8 mg/kg; Sigma, St. Louis, MO, USA) dissolved in 0.1 M phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed by scraping in a total volume of 80 µL of lysis buffer (containing 50 mM Tris-HCl pH 8, 150 mM NaCl, 0.5% sodium deoxycholate, 0.5% Triton-X 100, Sigma) and processed for immunoblotting as described in this manuscript under the PUM1 validation subchapter ...
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bioRxiv - Neuroscience 2021Quote: ... cells were scraped with 100 µL lysis buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 0.5% sodium deoxycholate, 0.5% Triton-X 100, Sigma) supplemented with EDTA-FREE cOmplete Mini protease inhibitor cocktail (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... Periorbital thresholds were assessed hourly for 8 hours following ethanol (300 mg/kg i.p. Sigma, St Louis, MO) administration as previously performed (Maxwell et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were prepared in two volumes (approx.8 ml) of CelLyticP buffer (Sigma-Aldrich, Cat No C2360) with 1 % of a Protease Inhibitor EDTA-free tablet (Roche ...
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bioRxiv - Microbiology 2021Quote: ... followed by pH 8-buffered 25:24:1 phenol:chloroform:isomyl alcohol and 24:1 chloroform:isomyl alcohol (Sigma-Aldrich®). Extracts were concentrated using 100 kDa membrane centrifugation (Amicon®) ...
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bioRxiv - Microbiology 2021Quote: ... sliced into cryosections (8-μm-thick) and stained with Oil Red O (Sigma-Aldrich, St. Louis, MO, USA) to assess the fatty droplet content ...
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bioRxiv - Microbiology 2021Quote: ... 8-10 seeds were plated on pre-dried 1% MS-agar (Murashige and Skoog basal salts mixture; Sigma) (2.2 g l−1 ...
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bioRxiv - Microbiology 2021Quote: ... were transduced with the lentiviruses at 0.3 infectious units/cell diluted in 8 μg/ml polybrene (EMD Millipore) as previously described ...
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bioRxiv - Microbiology 2021Quote: ... were transduced with the lentivirus at 0.3 infectious units/cell diluted in 8 μg/ml polybrene (EMD Millipore) as previously described ...
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bioRxiv - Microbiology 2021Quote: ... 4T1 cells were incubated in the filtrated viral supernatant in the presence of 8 μg/ml polybrene (Sigma) for 24 h ...
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bioRxiv - Cancer Biology 2021Quote: ... The migration ability of RAW 264.7 cells was examined using indirect transwell cell culture system (8 μm, Millipore). 4 × 105 of ID8 cells were seeded in 6-well plates evenly ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 8% fetal calf serum (FBS; CapriCorn Scientific) and 100 U/ml of penicillin/streptomycin (Sigma-Aldrich). All cell cultures were maintained at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... 8-week-old mice were anesthetized by isofluorane (CDMV) inhalation and 50 μl of 10 μM ctx (Sigma) was injected into the TA muscle ...
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bioRxiv - Cell Biology 2021Quote: ... and used to infect HeLa cells in the presence of 8 μg/ml hexadimethrine bromide (Sigma-Aldrich, #H9268). After 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... Adherent HEMs or MNT1 cells were incubated with viral particles and 8 μg/ml polybrene (H9268, Sigma-Aldrich) for 24 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated by 8-12% SDS-PAGE and transferred to PVDF membranes (Millipore-Merck). Membranes were incubated with primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Free Biotin-14-dCTP was removed by extensive buffer exchange with TE buffer (10 mM Tris.HCl pH 8, 0.1 mM EDTA) in a 100 kDa Amicon Ultra spin concentrator (Millipore). The biotinylated DNA was then concentrated to ∼10 mg/ml and stored at −20 °C until needed.
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bioRxiv - Microbiology 2022Quote: ... 16HBE cells were grown on 8-well glass slides (Nunc Lab-Tek II chamber slide system; Sigma-Aldrich) with each well seeded with 450 µl of cell suspension at 1.2 x 105 cells/ml and grown for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated in Tris-buffer (0.5 M, pH 8; TB) containing 0.025% diaminobenzidine solution (DAB wt/vol, Sigma) and 0.5% nickel ammonium sulfate (wt/vol ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 µg retina protein extracts were separated on 8% polyacrylamide gels and blotted onto PVDF transfer membranes (Millipore) followed by blocking with non-fat dried milk (Applichem) ...
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bioRxiv - Molecular Biology 2022Quote: ... 8 M urea and transferred onto a 30 kDa molecular weight cut-off column (Microcon-30, Merck Millipore). 150 μL 100 mM TRIS/HCl pH 8.5 ...
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bioRxiv - Molecular Biology 2022Quote: EBIV were first concentrated by adding 3.2 g polyethylene glycol (PEG) 8000 (8%, W/V, MilliporePEG8000, Sigma, USA) and 0.9 g NaCl (0.3 mol/L ...
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bioRxiv - Cell Biology 2022Quote: ... RPE1 cells were infected with Lentiviral packaged particles by supplementing 8 μg/ml protamine sulfate (P4505; Sigma-Aldrich), the virus infected cells were selected by overnight puromycin (7 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... were transduced with inducible STEMCCA and rtTA lentivirus–containing supernatants overnight in polybrene (8 μg/ml; Sigma-Aldrich). Cells were expanded and transduced with pLKO viruses expressing control shRNA or shRNAs targeting SOX15 ...
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bioRxiv - Cell Biology 2022Quote: ... Hexa KO cells were treated with the retrovirus containing medium and 8 μg/mL polybrene (Sigma-Aldrich, H9268). After 2 days ...
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bioRxiv - Immunology 2021Quote: ... 6-8 week old 2D2 FRP+ mice were immunized with 0.5 mg of mMOGtag in CFA (Sigma-Aldrich) s.c ...
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bioRxiv - Neuroscience 2020Quote: ... mouse primary microglia were seeded at 0.15×106/well in 8-well chamber Millicell EZ slides (Millipore PEZGS0816) and allowed to attach overnight ...
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bioRxiv - Microbiology 2020Quote: ... DNA was eluted from the beads in 50 μl 1x TE buffer (pH 8, Sigma Aldrich, MO, USA) at 35°C for 10 min ...
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bioRxiv - Bioengineering 2020Quote: ... Transduction was carried out using CMV-GFP lentiviruses in growth media with 8 μg/ml polybrene (Sigma-Aldrich) for 3-4 hours.
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... was coated and the previously mentioned cytokines were added in combination with polybrene (8 µg/mL, Sigma-Aldrich). The latter was only used in case of lentiviral transduction and it was removed after 24 hours by replacing medium and cytokines ...
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bioRxiv - Developmental Biology 2022Quote: ... 6-8 weeks old mice were intraperitoneal (i.p) injected with 200 mg/kg tamoxifen (TAM, Sigma-Aldrich, T5648) for 5 consecutive days ...
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bioRxiv - Biochemistry 2019Quote: Clone 8 cells were grown on 10 cm plates in Shields and Sang M3 insect Medium (Sigma #S8398) supplemented with 0.5 mg/mL insulin (Sigma #I6634) ...
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bioRxiv - Cancer Biology 2019Quote: ... F9 and WEHI-164 tumors was studied on ice-cold acetone-fixed 8-10μm cryostat sections stained with FITC labeled IgG(L19) (FITC_F7250 Sigma) (final concentration 2μg/mL ...
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bioRxiv - Genetics 2019Quote: ... Target cells were transduced with the resulting lentiviruses in the presence of 8 μg/mL polybrene (Sigma-Aldrich). At 24 hrs after transduction ...
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bioRxiv - Neuroscience 2019Quote: ... and cultured with and split 1:6 to 1:8 every four to five days using Accutase (Sigma). 10 µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Pathology 2020Quote: ... Male mice at 8-10-week old were intraperitoneally injected with Tamoxifen (1mg/kg, Sigma, St Louis, MO) for five consecutive days to induce Cre nuclear translocation and gene excision ...
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bioRxiv - Cell Biology 2021Quote: To measure symplasmic connectivity using the HPTS (8-Hydroxypyrene-1, 3, 6-trisulfonic acid trisodium salt, SIGMA-ALDRICH) dye movement assay ...
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bioRxiv - Developmental Biology 2019Quote: ... The viral supernatant was mixed with 8 μg/ml DEAE-dextran and 1000 units/ml ESGRO (Merck Millipore) and added directly to the E14tg2a cells ...
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bioRxiv - Immunology 2020Quote: ... Culture media was replaced with retroviral supernatant supplemented with of 50μM BME and 8 μg/mL polybrene (Millipore), and cells were centrifuged for 60min at 2000rpm ...
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bioRxiv - Synthetic Biology 2020Quote: ... adjusted to pH = 8 with 2 M NaOH and syringe-filtered (Millex® MP 0.22 μm, Millipore Sigma). Extreme caution was taken to prohibit contamination ...