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Citations for Millipore Sigma :
3451 - 3500 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... and peptide peak area ratios were generated against a spiked internal standard with bovine insulin (Sigma – 11070-73-8).
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bioRxiv - Molecular Biology 2024Quote: ... and the FANCI-FANCD2 complex was eluted with FLAG wash buffer containing 100 µg/mL 3X FLAG peptide (Sigma) and 5% glycerol ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... dried peptides were resuspended in 200 mM ammonium formate adjusted to pH 10 with ammonium hydroxide solution (Sigma-Aldrich). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... The pulldowns were eluted from the FLAG and Strep resins using 0.1 mg/mL 3xFLAG peptide (GlpBio) or 2.5 mM desthiobiotin (Sigma-Aldrich), respectively ...
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bioRxiv - Plant Biology 2023Quote: ... TF complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore F4799). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher 88843 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 100 µM Na3VO4) before the protein was eluted with FLAG wash buffer containing 0.5 mg/mL 3xFLAG peptide (Sigma). LIMK1 concentration was determined by running alongside BSA standards on SDS-PAGE with Coomassie staining.
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bioRxiv - Molecular Biology 2023Quote: The FLAG-tagged peptide and Pgk1 were detected using the anti-FLAG M2 (Sigma; cat# F1365; 1:1000 dilution) and anti-Pgk1 22C5D8 (abcam ...
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bioRxiv - Cell Biology 2023Quote: ... The resin was then washed with 3x10 bed volumes of lysis buffer and eluted with 800 µL (for 60 ml of cell suspension) lysis buffer (without protease inhibitor) supplemented with 0.2 mg/mL 3x Flag peptides (Sigma). The N-terminal 3xFlag tag was removed by incubation with the GST tagged Prescission Protease (GenScript ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were washed three times with buffer E and then mixed with 60 μg of 3xFLAG peptide (SIGMA) in 60 μl of buffer E for 1 h with shaking at 1400 rpm at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... with molar ratio of 1:5 (Nucleosome:SUV420H1) or 1:100 (Nucleosome: SUV420H1 C-terminal peptide) in the presence of 40 µM of SAM (Sigma). Samples were incubated on ice for 20 mins ...
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bioRxiv - Biophysics 2023Quote: ... The beads were washed 3 × 1 mL with membrane resuspension buffer for KRas before the beads were eluted with membrane resuspension buffer supplemented with 0.2 mg/mL 3X FLAG peptide (SAE0194, Sigma). Aliquots of samples were collected at each stage (input to anti-FLAG resin ...
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bioRxiv - Cell Biology 2023Quote: ... and FLAG-Sox4 protein was eluted by 5 rounds of competitive elution with 1× column volume (0.6 ml) of 100 μg/ml FLAG peptide (Sigma) in TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein was eluted with 4 CVs of imidazole-free wash buffer supplemented with 150 µg/mL 3xFLAG peptide (Sigma). This was analysed by SDS-PAGE before being concentrated ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein complex was eluted with 500 μL elution buffer (0.4 mg/ml 3X Flag peptide (Millipore Sigma F4799) in wash buffer ...
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bioRxiv - Biochemistry 2023Quote: ... dried peptides were dissolved in 20 μl of 2% formic acid supplemented with 20 mM citric acid (Sigma-Aldrich). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... The obtained beads fraction was resuspended with 180 μl of lysis buffer containing Influenza Hemagglutinin (HA) -peptide (Sigma-Aldrich) at the final concentration of 0.2 mg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... The target protein was eluted twice by incubating the matrix with 0.5 mL of K buffer supplemented with 200 μg/mL FLAG peptide (Sigma) for 0.5 h ...
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bioRxiv - Molecular Biology 2024Quote: ... The column was then washed 10 times with 1x Polymix buffer before elution in 200 µl of 1x Polymix supplemented with 0.1 mg/mL FLAG3 peptide (Sigma). 200 µl of the input lysate was saved and incubated at 4°C to use as a control for 5PSeq analysis of pulldown samples ...
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bioRxiv - Immunology 2024Quote: ... T2 cells were incubated with a titration of each tested peptide and 100 ng/mL human β2-microglobulin (Sigma) in serum-free medium (TexMacs ...
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bioRxiv - Cell Biology 2020Quote: ... BSA was coupled to the PEG (3000) for 1h at RT with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 100 mM, Sigma Aldrich, 39391) and N-hydroxy-succinimide (NHS ...
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bioRxiv - Cell Biology 2019Quote: ... were treated for 20 min at room temperature (RT) with 200 µl of Bind-silane solution—a mixture of 714 µl 3-(Trimethoxysilyl)propyl methacrylate (3-TMP, Sigma-Aldrich, M6514), 714 µl of acetic acid in 10 ml of 96% ethanol ...
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bioRxiv - Immunology 2021Quote: ... Cloning of the antigen library into the T7 phages was performed following manufacturer’s instructions (T7Select 10-3 cloning kit, catalog no. 70550-3, Merck-Millipore, Burlington, MA, USA).
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bioRxiv - Neuroscience 2019Quote: ... samples were washed 3 × 3 minute with PBS followed by a second 30-minute fixation step in 1% osmium tetroxide (Sigma-Aldrich, 75632). A final 3 × 3 minute PBS wash step was performed before samples were serially dehydrated in ethanol with a final 30-minute dehydration step using hexamethyldisilazane (HMDS ...
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bioRxiv - Biochemistry 2019Quote: ... Knock-down of glutamate dehydrogenase 2 (GDH2, encoded by the GLUD2 gene) using siRNAs (#NM_012084: 5’CUAACCUCUUCACGUGUAA’3 and 5’UUACACGUGAAGAGGUUAG‘3, Sigma-Aldrich, Taufkirchen, Germany) or transfection of GLUD2 plasmid/empty vector controls was done using Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... to 30 µL CSF (pooled from 1 – 3 animals) and placing in 3 mm NMR tubes (Wilmad®, 7 inch; Sigma-Aldrich). Serum samples (samples pooled correspondingly to the CSF samples ...
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bioRxiv - Neuroscience 2020Quote: ... FBS supplementation was decreased to 3% FBS 3 days prior to the addition of 10□M Retinoic Acid (RA; Sigma-Aldrich, R2625). During differentiation ...
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Microbiology 2020Quote: ... ACE2 (15 μg/mL) was immobilized on AR2G biosensors activated by 1-ethyl-3-[3-dimethylaminopropy] carbodiimide hydrochloride (EDC, E1769, Sigma, Shanghai, CHN) and sulfo-N-hydroxysulfosuccinimide (s-NHS ...
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bioRxiv - Microbiology 2021Quote: Viral stocks of molecular clones HIV-1NL4-3 and HIV-1NL4-3 BaL (AIDS Reagents Program) were generated by transfecting HEK293T cells using PEI transfection reagent (Sigma, Cat: 408727). Pseudotyped HIV-1VSVg-NL4-3 ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was isolated from 27 samples (‘EMEK3’, R bulk and S bulk × 3 treatments/ sampling time × 3 replicates) using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Complementary guide oligonucleotides (forward: 5’-CACCGCAGGATTGAAGACCTTAACA-3’; reverse: 5’-AAACTGTTAAGCTCTTCAATCCTGC-3’) were custom synthesized separately by Sigma-Aldrich (St. Louis, MO, USA), annealed ...
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bioRxiv - Biophysics 2021Quote: ... The primers used for the same were a) A350P forward 5’-gctgcatccggccgcatctcggc-3’ and b) A350P reverse 5’-gccgagatgcggccaaggatgcagc-3’ (Sigma Aldrich, India). Full length A350P was thereafter cloned into an empty pEGFP (Clontech ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the AgNP suspension was washed with deionized (DI) water by ultrafiltration through a membrane with pore size of 3 kDa (Amicon Ultracel 3 KDa Ultrafiltration Discs, Millipore, Billerica, USA) under nitrogen gas pressure (1 bar ...
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bioRxiv - Cell Biology 2020Quote: ... Then the cells were washed with PBS 3 times and incubated with cyanine 3 (Cy3)-conjugated donkey anti-goat IgG secondary antibody (1:500, Millipore, Temecula, CA) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hrs followed by addition of 3 µM Asunaprevir (Apexio: BMS-650032) and 500 µM 3-Indoleacetic acid (Sigma Aldrich: I2886). Cells were treated with following inhibitors to inhibit indicated kinases ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples were loaded onto a Supelco™ Discovery™ HS F5-3 (15 cm×2.1 mm, 3-μm) column (Sigma-Aldrich Co.) kept at 30 °C ...
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bioRxiv - Bioengineering 2023Quote: ... A 0.5 M stock solution of inducer was prepared by solubilizing 3-methyl-benzoate (3-MB) (Sigma-Aldrich, St Louis, MO, USA) in 0.5 M NaOH.
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bioRxiv - Systems Biology 2022Quote: ... The His6-MBP or His6-MBP-14-3-3 concentrations on the resin were determined by pipetting 3×200 μL of diluted resin (corresponding to 3×15 μL of resin) into a filter plate (Millipore, Burlington, Massachusetts), filtering out the buffer by centrifugation (500g) ...
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bioRxiv - Genetics 2023Quote: ... Single clones were generated and screened for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich, D9891, 3 µg/ml for 3 days) via Western Blot and flow cytometry after intracellular staining using anti-Flag antibody (Sigma Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Chemical compounds were first identified using the NIST library and later confirmed with co-elution of synthetic 4-methyl-3-heptanone (Pfaltz and Bauer M19160) and 4-methyl-3-heptanol (Sigma Aldrich M48309). Compounds eluting after 30 minutes were excluded from the analysis due to lack of volatility.
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bioRxiv - Developmental Biology 2023Quote: ... oocytes are first isolated from ovaries by short enzymatic digestion (3 mg/ml Collagenase I, Sigma-Aldrich, #C0130; 3 mg/ml Collagenase II, Sigma-Aldrich, #C6885 ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were probed overnight at 4°C with anti-HA antibody (MBL, M180-3) and anti-glyceraldehyde-3-phosphate dehydrogenase antibody (Sigma-Aldrich, G8795) or with anti-dystrophin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... The extract was reconstituted in a 500-μl solution of methanol/water (1:1) and filtered through a 3-kDa molecular filter (Amicon® Ultra Centrifugal Filter, 3 kDa MWCO, Millipore Sigma) at 4°C for 90 minutes to remove proteins ...
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bioRxiv - Biochemistry 2024Quote: ... 8029.3), and 0.5% sodium deoxycholate (AppliChem, A1531) in PBS supplemented with Phosphatase Inhibitor Cocktail 2 and Cocktail 3 (Sigma-Aldrich, P5726, P0044) and Complete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Plant Biology 2019Quote: ... 20 μL/mL Phosphatase Inhibitor Cocktail 3 (Sigma, P0044), 20 μL/mL Phosphatase Inhibitor Cocktail 2 (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... and Pellet Paint Co-precipitant (Merck Millipore #69049-3). Genomic DNA traces were removed with DNA-free DNase Treatment and Removal Reagents (FisherScientific #AM1906 ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μM 3-Isobutyl-1-methylxanthine (IBMX, Sigma, #I7018) was supplemented to the media for the indicated time ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1% of phosphatase inhibitor cocktail 3 (Sigma-Aldrich) was added to the adherent cells and permitted to incubate on ice for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... oligonucleotides carrying a 3’ Cy5 fluorophore (purchased from Sigma) were ligated subsequently ...
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bioRxiv - Cancer Biology 2021Quote: ... and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich), for 1 hour at 4°C on rotation ...