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Citations for Millipore Sigma :
3351 - 3400 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... membranes were incubated overnight at 4°C with primary antibodies in a 1:1 solution of TBST (TBS + 0.05% Tween20) and LiCor blocking buffer (Rabbit anti-GFP, Torrey Pines Scientific TP401; and Mouse anti-alpha-tubulin, Sigma T5168). Membranes were then washed 5x times with TBST ...
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bioRxiv - Neuroscience 2023Quote: ... After blocking tissue with 5% swine serum and 2% BSA in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, # T8787), immunofluorescence was performed by overnight incubation at 4 °C with a rabbit anti-HA primary antibody (Cell Signaling ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS for 30 min and exposed to blocking buffer (2 wt% FBS, 0.5 wt% bovine serum albumin (Sigma Aldrich) and 0.1 wt% Tween-20 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 7.5ml Intercept PBS blocking buffer with 0.1% Tween (SynGAP1; 1:1000dil, Invitrogen, PA1-046, Gapdh; 1:15000, Sigma-Aldrich G8795). Membranes were incubated with the primary antibody solution overnight at 4[ ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted 1:10000 in Intercept Blocking Buffer for 1.5 hour at room temperature or HRP-conjugate antibodies goat anti-rabbit IgG (A0545, Sigma-Aldrich) and anti-mouse IgG (A4416 ...
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bioRxiv - Molecular Biology 2023Quote: ... in PBS for 5 minutes at room temperature and the non-specific binding of the antibodies was prevented by applying a blocking solution (PBS supplemented with 5% BSA (Sigma) or 5% goat serum (for Aurora B kinase immunostaining in heart sections ...
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bioRxiv - Molecular Biology 2023Quote: ... a DNA hydrolysis step between the permeabilization and the blocking step was performed by the addition of 2M HCL (Sigma) for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... All primary antibodies were incubated overnight with membranes in TBS blocking buffer supplemented with 0.1% Tween-20 (Sigma-Aldrich # P1379), while secondary antibodies (HRP conjugate ...
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bioRxiv - Biophysics 2023Quote: ... Cells were resuspended in 100 µl FACS blocking buffer (phosphate buffered saline (PBS) supplemented with 5% (w/v) bovine serum albumin (BSA) (Sigma)) and centrifuged at 400g for 5 min at 25°C ...
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bioRxiv - Biochemistry 2022Quote: ... blocked with SMB plus 1.5 mg/mL Roche Blocking Reagent and 2 mg/mL poly-glutamic acid (1500-5500 Da., Sigma-Aldrich); and then 4 ...
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bioRxiv - Bioengineering 2023Quote: ... To block non-specific binding antibodies, samples were exposed to Blocking Buffer Solution (BBS, composed with 0.5% fetal bovine serum (Sigma-Aldrich), 0.3% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Brain sections were post-fixed with 4% PFA for 10 minutes and then washed with PBS three times and incubated 30 minutes with blocking solution (PBS/0.1% Triton X-100 containing 10% normal goat serum (Sigma-Aldrich)) and then incubation overnight at 4°C in blocking solution with primary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... cryosections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
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bioRxiv - Immunology 2023Quote: ... in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin, Sigma-Aldrich) for 40 minutes at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and were then incubated in blocking solution consisting of TBS containing 2% (v/v) normal donkey serum (Catalog # S30–100ML; EMD-Millipore), and 0.1% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... permeabilised by washing in 0.1% Triton-X in PBS (PBS-T) for 15 min and incubated for 2 h in a blocking buffer of 2% bovine serum albumin (Sigma) and 3% normal donkey serum (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... After blocking with 5% BSA the membrane was incubated for 1 h with 50 µg of purified PLG (Sigma-Aldrich) diluted in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed twice with Tris-buffered saline (TBS) and were blocked with blocking buffer (TBS containing 5% donkey serum (Biozol) and 0.25% Triton X-100 (Sigma-Aldrich)) for 1h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were then washed 3 times for 20 min in PBS-Triton before incubation with anti-rabbit Alexa Fluor 546 used at 1:500 dilution in blocking buffer containing Hoechst 33342 (Sigma) to label the nuclei ...
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bioRxiv - Neuroscience 2023Quote: ... for 2h in a 10% fetal bovine serum (FBS)/PBS blocking buffer containing 0.5% Triton X-100 (cat# X100, Sigma-Aldrich), and incubated overnight at 4°C with the following primary antibodies diluted in the blocking buffer ...
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bioRxiv - Cell Biology 2024Quote: ... then 50%/50%, then 25%/75%) then incubated in blocking solution (0.3% Triton-X100, 0.45% fish gelatin (Sigma G7765-250ML), 0.6% IgG free BSA (Jackson ImmunoResearch 001-000-162 ...
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bioRxiv - Cell Biology 2024Quote: ... Alfa Aesa) for 10 min and subsequently washed with PBS before blocking with CT (0.5% Triton X-100 (Sigma-Aldrich), 5% ChemiBLOCKER (Merck Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... The antibodies for spinal cord staining were diluted in blocking solution prepared with 5 % Normal Donkey Serum (Millipore #S30-M) and 0.5 % Triton-X in 1 x PBS.
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bioRxiv - Neuroscience 2024Quote: ... for 5 x 7 min and incubated 2h at room temperature (RT) with blocking solution containing 10% normal donkey serum (NDS, Merck Millipore), 3% Bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: Sections were first incubated with a blocking solution made with 5% normal donkey serum and 0.3% TritonX-100 (Sigma-Aldrich) in a PB solution for 1h to prevent the non-specific binding of antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with a blocking solution made with 5% normal goat serum (NGS) and 0.3% TritonX-100 (Sigma-Aldrich) in a PB solution for 1h to prevent the non-specific binding of antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then rinsed with rinsed with 0.1% Triton X-100 in PBS (0.1%PBST) and then blocked with blocking buffer consisting of 5% normal goat serum (G9023, Sigma-Aldrich) in 0.1% PBST for 1hr at RT ...
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bioRxiv - Bioengineering 2023Quote: ... The gels were then washed with wash buffer three times for 5 min followed by permeabilization and blocking in 5% BSA (Sigma), 1% Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated with blocking solution (0.5% Triton X, 2.5% bovine serum albumin [Sigma-Aldrich], 2.5% donkey serum [Sigma-Aldrich]) for one day at 37 °C before being incubated with the primary antibody at 1:500 for 3 days at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... for 15 min and then cell blocking solution (5% w/v BSA, purchased from Millipore Sigma, dissolved in 1x DPBS) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated with primary antibodies diluted in blocking solution (anti-MAP2, 1:500, chicken, ab5392, Abcam; and anti-vGlut1 1:1000, guinea pig, AB5905, Merck Millipore) for 2 h at RT ...
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bioRxiv - Immunology 2024Quote: ... Cultures were washed with 4% FBS in PBS and incubated with blocking solution (2% FBS, 2% bovine serum albumin [BSA, A2153; Sigma] ...
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bioRxiv - Immunology 2024Quote: ... Cell permeabilization was performed for 10 min with 0.25% Triton X-100 in PBS followed by blocking with 5% normal goat serum (Sigma- Aldrich) for 2 h at room temperature and overnight incubation with anti-CD3 (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... incubated for 1h at RT in blocking buffer (BB: 10% Bovine Serum Albumin 0.3% Triton™X-100 (Sigma-Aldrich) in 1X PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore; sheep anti-TH ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in primary antibody (Rabbit anti-GFP 1:1000 in blocking buffer, Cat# AB3080, Millipore Sigma, St. Louis, MO) overnight at room temperature on an orbital shaker ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue slides sectioned from the anterior of the hippocampus containing typical dentate gyrus structures were first incubated in blocking and permeation solution with 2% Triton X-100 (Sigma) for 2 h ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were immersed in blocking solution (5% (vol/vol) horse serum in phosphate buffer with 0.1% (vol/vol) Triton X-100 (Sigma; X100)) for 1 h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Permeabilization was then performed by addition of a solution of 0.5% Triton X-100 (AppliChem, A1388,0500) for 30 min before addition of blocking solution (BSA 1% (Sigma-Aldrich, A7906) in DPBS ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed 3 times for 20 - 40 minutes each wash in PBS before incubation with secondary antibody(s) in blocking solution for 2 - 4 hours at room temperature (Alexa 647-conjugated donkey anti-chicken, AP194SA6, Millipore – to label GFP ...
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bioRxiv - Neuroscience 2024Quote: ... slices underwent a 2h incubation at room temperature (RT) in a blocking solution consisting of 0.1M PBS supplemented with 5% (v/v) goat serum (Sigma; G9023) and 0.3% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... Sections then were incubated in blocking buffer (10% normal donkey serum in 0.5% PBST, Sigma-Aldrich, catalog no: D9663-10ML) for 1 hour at RT before incubating in primary antibody solution (2% normal donkey serum in 0.4% PBST ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were permeabilized with 0.5% Triton-X100 in PBS for 5 min at RT and a blocking solution (PBS supplemented with 5% BSA (Sigma) and 0.1% Triton-X100 ...
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bioRxiv - Microbiology 2024Quote: ... was separated from the insert and blocked and permeabilized with a blocking buffer containing 2% Bovine Serum Albumin (BSA) (Serva) and 0.1% saponin (Sigma Aldrich) before incubating with the primary and secondary antibodies at 4°C in dark ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were blocked and permeabilized in blocking buffer (10% donkey serum [Jackson ImmunoResearch 017-000-121] in PBS with 0.3% Triton [Sigma T9284] and 100mM L-lysine [Sigma L5501] ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a 1 h incubation with secondary antibodies also diluted in blocking buffer8 Immunostained tissue sections were counterstained with DAPI (1:1000 in PBS) and were mounted in Mowiol (Millipore). Other immunohistochemical stainings were performed as previously described52 using antigen retrieval in 0.01 M sodium citrate buffer (pH 6.0 ...
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bioRxiv - Biophysics 2020Quote: The lyophilized linear peptide (4.1 mg, 1 μmol) was dissolved in 100 ml refolding buffer containing 100 μmol reduced glutathione (GSH, Sigma), 10 μmol oxidized glutathione (GSSG ...
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bioRxiv - Cell Biology 2019Quote: ... beads were washed 3X with 0.1% Tween20/TBS and 3X with TBS before elution with 3XFLAG peptide (Sigma F4799). Gel loading buffer was added to the eluates for SDS-PAGE electrophoresis and Western blotting analysis.
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bioRxiv - Cell Biology 2020Quote: ... digested sample was dried in a vacuum concentrator and reconstituted in 0.1% TFA + 1 picomole/uL Angiotensin-II peptide (Sigma). 1% of the sample was subjected to LC-MS/MS/MS analysis on an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Bound FLAG-HA-tagged KAT6B and associated proteins were eluted with 0.3 μg/μl 3x FLAG tag peptide (Sigma) in BC100 buffer for 1 h with gentle rotation at 4°C.