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Citations for Millipore Sigma :
3251 - 3300 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... (1) A modification solution was made by dissolving 10 mM of 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC, Sigma-Aldrich Inc.) and 75 mM of N-hydroxysuccinimide (NHS ...
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bioRxiv - Biochemistry 2023Quote: Two complementary single strand oligonucleotides (5’-GTAATCTGGGCCACCTGCCTGGGAGGA-3’ and 5’-TCCTCCCAGGCAGGTGGCCCAGATTAC-3’) corresponding E2 box44 were Two complementary single strand oligonucleotides (5’-purchased from Sigma-Aldrich. An equal molar ratio of single strand oligonucleotides was mixed and incubated at 95°C for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... HEK 293FT cells were transfected with 1μg of TRC-pLKO.1-Puro plasmid containing either non-targeting shRNA (5’-CAACAAGATGAAGAGCACCAA-3’) or ATF4-targeted shRNA (5’-GCCTAGGTCTCTTAGATGATT-3’) (Sigma-Aldrich), together with 1 μg mixture of packaging plasmids (pMD2.G and psPAX2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with either Odyssey Blocking Buffer (Licor Cat #927-40000) or 5% skimmed milk in TBST buffer (SIGMA) and incubated for 1h at room temperature or overnight at 4° ...
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bioRxiv - Developmental Biology 2021Quote: ... the embryos were incubated overnight with an AP-coupled anti-DIG antibody in the blocking solution and finally stained with NBT/BCIP liquid substrate (Sigma). After the staining ...
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bioRxiv - Developmental Biology 2021Quote: Newborn pups were injected at P2 via the temporal vein with 0.0025mg/g of DLL4-neutralizing (blocking antibody (YW152F, Genentech (31)) or IgG from human serum (cat #I4506, Sigma Aldrich). The pups were sacrificed 48 h after injection at P4 ...
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bioRxiv - Biophysics 2020Quote: ... The membrane was incubated for 1 h in a blocking buffer of PBST (PBS supplemented with 0.1 v/v % Tween) and 10 % (v/w) dry skim milk (Sigma-Aldrich). The membrane was washed 3 times with PBST for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were permeabilized with 0.5 % TritonX-100 (Wako) in 1x PBS for 1-2 h and blocked in blocking solution (2 % BSA (Sigma-Aldrich), 1 % DMSO ...
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bioRxiv - Developmental Biology 2021Quote: ... Rigid paralysis of the skeletal muscles was induced by treatment with the neuromuscular blocking agent decamethonium bromide (DMB) (Sigma Aldrich) (Bowman and Rand ...
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bioRxiv - Plant Biology 2022Quote: ... following an hour of primary antibody incubation in Intercept® (PBS) Blocking Buffer with 0.2% Tween® 20 (Sigma, P1379) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... For immunofluorescence stainings slices were incubated for 1 h at room temperature (RT) in blocking buffer (2% gelatine, 2% BSA (bovine serum albumin, Cat#a2153, CAS Number: 9048-46-8, Sigma), 0.1% Triton X-100 (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... Whole brains were dissected and incubated in blocking solution (5% normal donkey serum and 1% Bovine Serum Albumin (BSA; Sigma) in PBS with 0.1% Triton-X100 (PBS-T) ...
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bioRxiv - Immunology 2021Quote: ... for 10 min at room temperature followed by 1 h blocking step with PBS containing 0.4% Triton X-100 5% goat serum (all Sigma-Aldrich) before incubation overnight at 4°C with anti-Muc2 (Novus Biologicals #NBP1-31231 ...
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bioRxiv - Immunology 2019Quote: ... saturated in blocking solution (PBS-BSA 5%) for 1 hour and finally incubated with primary Abs: rabbit anti-mouse laminin (Sigma), Alexa fluor 594 Goat anti-mouse IgM (Thermofisher ...
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bioRxiv - Physiology 2020Quote: ... followed by antigen retrieval in citrate buffer (10 mM, pH=6.0) for 40 min in 90□ and blocking in 2% BSA (A2153, Sigma Aldrich) with 0,3 M glycine (G8898 ...
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bioRxiv - Immunology 2019Quote: ... This was followed by incubation for 1 hour at room temperature in blocking buffer consisting of 2% goat serum (Sigma) in PBS 0.1% Triton X-100 ...
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bioRxiv - Developmental Biology 2019Quote: ... Antibody and chemical staining were performed in blocking solution at the following dilutions: anti-β-catenin 1:500 (Sigma C2206), anti-laminin 1:500 (Sigma L9393) ...
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bioRxiv - Genomics 2019Quote: ... Cells were then incubated with a STAT3 antibody (9139, Cell Signalling) in blocking buffer + 0.1% tween-20 (P1379, Sigma Aldrich) for 1-2 hours at room temperature or overnight at 4°C on a shaker ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated with 0.5% Triton and blocked with 0.5% Triton and goat serum blocking solution before being incubated in mouse anti-PV monoclonal antibody (1:2000, Sigma-Aldrich) and mouse anti-NeuN polyclonal antibody (1:2000 ...
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bioRxiv - Molecular Biology 2020Quote: Sections were incubator in PBS for 10 min and then blocked with blocking buffer (5% Bovine Serum Albumin (BSA, Sigma) in PBS with 0.1% Triton X-100) ...
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bioRxiv - Neuroscience 2020Quote: ... Spontaneous IPSCs were isolated by blocking AMPA receptor-mediated events with CNQX (6-cyano-7-nitroquinoxaline-2,3-dione; 20uM; Sigma-Aldrich). Miniature events were isolated by blocking sodium channels with the addition of tetrodotoxin (1uM ...
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bioRxiv - Bioengineering 2021Quote: ... then incubated in blocking buffer containing 1% (w/v) BSA and 10% (v/v) goat serum (all from Sigma-Aldrich) in 1X PBS for 1 hour at room temperature (RT ...
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bioRxiv - Neuroscience 2020Quote: ... were coated for 2h with 5.0 µg/ml monoclonal GR antibody followed by overnight blocking with 1% BSA in PBS-Tween (0.05% Tween-20, Sigma Aldrich) at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The blocking buffer was next replaced with rabbit polyclonal T22 antibody that recognises oligomeric Tau (diluted 1/2000) ((ABN454; Merck Millipore) (37) ...
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bioRxiv - Neuroscience 2021Quote: ... for 30 min prior to incubation for 1 h in primary antibodies diluted in blocking buffer: Goat anti-mouse GAPDH (RRID: AB_1078991, Sigma-Aldrich) 1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then blocked and permeabilized using a DPBS-based blocking buffer containing 0.1% (w/w) saponin (Sigma-Aldrich, S4521) and 5% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... then incubated with a 0.5% Triton X-100 solution and finally with casein blocking buffer (0.25% casein (Sigma, C3400-500G) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C in blocking buffer with the following primary antibodies: rat anti-MBP (monoclonal, 1:100, Millipore), rabbit anti-Parvalbumin (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... After blocking with Tris Buffered Saline with Tween 20 (TBST) containing 5% (w/v) bovine serum albumin (BSA) (Sigma, A3608) for 1 h ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... 500uL of a blocking solution consisting of PBS with 5% donkey serum (Jackson, #017-000-121) and 0.1% Triton X-100 (Sigma, #T8787) was added to the wells and incubated for one hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked in the blocking buffer of 5% BSA in TBST (137 mM NaCl, 2.7 mM KCl [Sigma-Aldrich P9541] ...
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bioRxiv - Cancer Biology 2021Quote: ... The chambers were gently washed twice with PBS and filled with 100μL of blocking solution - 2.5% (w/v) bovine serum albumin (Sigma-Aldrich, A9418) in PBS - and incubated for 1 hour at room temperature ...
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bioRxiv - Physiology 2022Quote: ... Cells were washed with PBS three times for 5 min and incubated with blocking buffer consisting of 5% goat serum (CST) and 0.3% Triton X-100 (Sigma Aldrich) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by blocking for 1 hour at room temperature with 4% skim milk powder (Sigma-Aldrich, St. Louis, MO, USA) in Tris-buffered saline pH 7.4 (TBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The primary antibody was then replaced with blocking solution containing 5 μg/mL bisBenzimide H 33342 trihydrochloride (Sigma-Aldrich, B2261) and goat anti-mouse Alexa Fluor 488 secondary antibody (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 times with PBS and incubated for 2 h with blocking and permeabilization buffer (1% BSA, 5% goat serum, 0.15% saponin (Sigma Aldrich)) ...
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bioRxiv - Neuroscience 2021Quote: ... at 40um thickness from postmortem human brain blocked for 1 hour at room temperature in blocking buffer before being incubated in primary antibodies for 72 hours at 4°C with gentle rocking in blocking buffer (Anti-NeuN, Synaptic Systems cat no. 266 004, 1:500; Anti-phospho-Histone H2A.X, Millipore cat no ...
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bioRxiv - Neuroscience 2021Quote: ... and APC rat anti-mouse 4D4 (marking resident microglia; 1:1000) in blocking buffer containing 0.2% bovine serum albumin (BSA, Sigma-Aldrich) in HBSS ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed in PBS before permeabilisation in 0.1% Triton X-100 (Fisher Bioreagents) and blocking in 1% bovine serum albumin (BSA; Fisher Bioreagents) and 10% normal goat serum (NGS; Sigma) in PBS for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... were equilibrated for >600 sec in 1x PBS prior to loading with his-tagged NiV prefusion F (25 µg/mL in Blocking Buffer (1x PBS, 1% BSA (Sigma)) for 600 sec ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4 °C with primary antibodies diluted in blocking buffer (anti-vGluT1, 1:1000, guinea pig, Millipore and anti-MAP2 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4 °C with primary antibodies diluted in blocking buffer (anti-vGluT1, 1:1000, guinea pig, Millipore and anti-MAP2, 1:1000, rabbit, Millipore). Sections were washed 3 times for 10 mins each in 1x PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in blocking buffer (5% non-fat milk or 5% FBS diluted in TBS-T buffer (0.05% Tween-20 (Sigma-Aldrich) in tris-buffered saline (TBS) ...
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bioRxiv - Neuroscience 2019Quote: ... Neurons were then treated with 10 mM glycine (Fisher BP381-1) in PBS, and incubated in a blocking solution (0.1% Triton X-100, 2% fetal calf serum (Sigma F4135), and 3% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2019Quote: ... cells were incubated in blocking buffer (TBS containing 5% goat serum, 1% Bovine Serum Albumin, and 0.1% Triton-X-100 (Sigma Aldrich)) for 45 min at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated overnight at room temperature in blocking buffer containing primary antibody (mouse anti-UBE3A (Sigma SAB1404508 clone 3E5)) at 1:250) ...
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bioRxiv - Microbiology 2020Quote: ... The ELISA plates were then blocked with 300 μL of blocking buffer (1% BSA and 10% bovine calf serum (BCS) (Sigma) in PBS at 37°C for 2 hrs ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were then incubated in blocking buffer (5% v/v NDS, 0.2% v/v fish gelatin (catalog no. G7765, Sigma-Aldrich), 0.2% v/v Tween-20 in PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were then probed with corresponding Li-Cor anti-mouse or anti-rabbit fluorescent secondary antibodies for one hour at room temperature at dilutions of 1/10 000 in Odyssey Blocking Buffer with 0.1% Tween-20 (Sigma-Aldrich) and 0.1% Tween ...
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bioRxiv - Bioengineering 2020Quote: ... All slices were blocked for 1hr at room temperature in blocking solution that contained 50% goat serum (Millipore S26-100ML) and 0.01% Triton X-100 (Sigma T9284 ...