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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Recombinant Human CD28 protein Biotin labelled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing recombinant proteins were incubated with anti-FLAG M2 affinity gel (Sigma, A2220) for 2 hours on a rotator at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... subcutaneously with 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, Germany) followed by a boost after 4 weeks ...
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bioRxiv - Plant Biology 2020Quote: ... The expression of recombinant protein was induced by 0.2% (w/v) L-Arabinose (Sigma Aldrich). The expressed protein was purified as His-tagged fusion protein using Ni-NTA agarose beads (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... The detection of recombinant proteins was analyzed by immunoblot using an anti-myc antibody (Sigma) for SyNOS and a specific OtNOS antibody generated by GenScript Company.
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bioRxiv - Neuroscience 2021Quote: ... The recombinant proteins were purified with anti-FLAG agarose affinity gel (A2220; Sigma-Aldrich Japan), eluted with FLAG peptide (A3290 ...
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bioRxiv - Microbiology 2022Quote: ... the recombinant protein was briefly vortexed in the presence of 1% Triton X-114 (Sigma), incubated on ice for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Flag-hSpt16 proteins were incubated with 12.5 U of recombinant ULP-1 SUMO Protease (Sigma) for 16 h at 4°C to remove SUMO moieties.
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bioRxiv - Molecular Biology 2023Quote: ... Purified recombinant proteins were analyzed by SDS-PAGE and concentrated using stirred ultrafiltration cells (Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Recombinant SARS-CoV-2 BA.1 nucleocapsid protein was subcloned into the pET46 vector (Novagen) with an upstream 6xHis tag and expressed in Rosetta2 pLysS E ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant proteins (PRMT5:MEP50 complex (SRP0145-25470) and EZH2 (SRP0379) were purchased from Sigma-Aldrich USA ...
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bioRxiv - Bioengineering 2022Quote: ... Epidermal growth factor (EGF) and Recombinant Human Fibroblast Growth Factor Basic (FGF2) Progesterone were purchased from Sigma-Aldrich. OPSS-PEG-SVA (3400 Da) ...
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bioRxiv - Immunology 2020Quote: ... Transduced CD8+ T cells were expanded in the presence of 10 U/ml recombinant human IL-2 (Sigma) Human CD8+ T cells were purified from donor PBMCs (Cambridge Bioscience or NHSBT ...
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bioRxiv - Cell Biology 2020Quote: ... the cell medium was replaced with DMEM containing 0.2% FBS and 300ng/mL IGF-1 (human recombinant, Sigma) for 3 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1 IU/mL insulin and 10 ng/mL human recombinant epidermal growth factor (EGF) (all from Sigma-Aldrich). TKCC10 cells were grown in a 1:1 mixture of M199 medium and Ham’s F12 medium ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with medium containing 10 µg/mL recombinant human adiponectin (SRP4901, Sigma-Aldrich, in sterile water), a quantity equivalent to typical adiponectin levels in lean women (Ding et al. ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Cell Biology 2023Quote: 2 μg GST-tagged NuSAP point mutants were incubated with 50 ng human recombinant Aurora A (Sigma-Aldrich) in a water bath at 30°C for 30 min in kinase buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were expanded in the presence of 10 U/mL recombinant human IL-2 (11147528001, Sigma). Human CD8+ T cells were purified from donor PBMCs (NHSBT or Karolinska Hospital ...
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bioRxiv - Microbiology 2022Quote: ... functional-component spacer diacyl lipid conjugated to biotin (FSL-biotin; Sigma-Aldrich), as described previously (43) ...
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bioRxiv - Biophysics 2020Quote: The RBD and spike proteins were chemically biotinylated using the EZ-Link NHS-PEG4-Biotin kit (Sigma). For the RBD ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were eluted by 2 mM biotin in buffer W and concentrated with 100kDa Amicon (Millipore). The concentration of dtSARM1 was determined by BCA assay (Thermo Fisher).
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bioRxiv - Biochemistry 2021Quote: ... recombinant MEK and recombinant ERK were purchased from Sigma. Indicated amounts of recombinant GST-HSF-1 (0.1–0.4 mg ...
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bioRxiv - Microbiology 2020Quote: ... ii) 300 μg C g−1 13C-labelled Algal crude protein extract-98 atom% 13C (ISOTEC, Sigma-Aldrich), iii ...
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bioRxiv - Cancer Biology 2021Quote: ... biotin (Sigma Aldrich, #B4501), lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and biotin-UTP (Sigma) according to the manufacturer’s instructions and purified by the MEGAclear spin columns (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Biotin was from Sigma.
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bioRxiv - Neuroscience 2021Quote: ... biotin-cadaverine (Sigma-Aldrich) 0.1 mM ...
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bioRxiv - Cell Biology 2022Quote: ... 33 mM biotin (Sigma), and 17 mM D-pantothenate (Sigma ...
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bioRxiv - Systems Biology 2022Quote: ... Biotin-agarose (Sigma, USA) was added to the reaction and incubated overnight to capture the pupylation linked interacting proteins ...
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bioRxiv - Systems Biology 2022Quote: ... Biotin-agarose (Sigma, USA) was added to the reaction and incubated overnight to capture the pupylation linked interacting proteins ...
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bioRxiv - Systems Biology 2022Quote: ... biotin agarose (Sigma, USA) was added to the supernatant and incubated overnight to capture the pupylation linked interacting proteins on cell surface ...
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bioRxiv - Systems Biology 2022Quote: ... Biotin-agarose (Sigma, USA) was added to the reaction and incubated overnight to capture the pupylation linked interacting proteins ...
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bioRxiv - Cell Biology 2022Quote: ... and biotin (Sigma-Aldrich) following a published protocol (Hyman et al. ...
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bioRxiv - Biochemistry 2019Quote: ... D-biotin (Sigma Aldrich) was added to a final concentration of 50 μM ...
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bioRxiv - Cell Biology 2021Quote: ... and Biotin (Sigma; B4639).
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bioRxiv - Microbiology 2023Quote: ... biotin (#B4639, Sigma- Aldrich), sodium arsenite (NaAsO2 ...
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bioRxiv - Biophysics 2023Quote: ... to Biotin (Merck Millipore) in a molar ratio of 1:3.5 at 500 µM Streptavidin and 1,750 µM Biotin in 100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... 33 mM biotin (Sigma), and 17 mM D-pantothenate (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin (Sigma, #B4501-100MG) was stored as a powder at room temperature and solubilized in serum-free DMEM to the indicated concentration immediately before use.
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bioRxiv - Cell Biology 2024Quote: ... 50 μΜ biotin (Sigma) and 1 μg/mL doxycycline in culture medium for a further 9 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... 5’-IRDye 800-labelled (Integrated DNA Technologies) or 3’-6FAM-labelled oligonucleotides (Sigma-Aldrich) were employed ...
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bioRxiv - Bioengineering 2019Quote: ... Lyophilized proteins were purchased: human serum albumin (HSA; from human plasma, ≤0.02% Fatty acids, Lot #SLBZ2785, Millipore Sigma) and fibrinogen (FBG ...
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bioRxiv - Cancer Biology 2019Quote: The uncharged polystyrene 96-well plate were coated with different recombinant proteins-Collagen IV (Merck Millipore), P-Selectin ...
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bioRxiv - Neuroscience 2022Quote: Fixation was performed following 24 hrs of recombinant protein treatment using 4 % PFA / 4% Sucrose (Sigma) in 1x PBS at RT for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant protein was concentrated by centrifugation in Amicon® Ultra-15-30K (Millipore Sigma, Burlington, MA), purified on protein A/G columns (NAb™ Spin Kit ...
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bioRxiv - Biochemistry 2024Quote: Recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Cell Biology 2022Quote: ... and addition of biotin supplemented media (3mM of D-biotin, B4501 Sigma-Aldrich), by using a magnetic imaging chamber with L-shape tubing (CM-B25-1PB ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich). After 24 hours of CHIR99021 stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... We dissected ovaries in live imaging media (Schneider’s Drosophila medium containing 15% fetal bovine serum (FBS) and 200 mg/mL recombinant human insulin (Sigma)) ...