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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Recombinant Human CD28 protein Biotin labelled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: Recombinant proteins were expressed in Escherichia coli BL21(DE3)pLys using pET28a+ (Novagen) and purified using Ni-Sepharose affinity medium (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant proteins were produced and purified on glutathione-agarose column (G4510, Sigma) for GST-tagged protein or on TALON-Cobalt beads column (635507 ...
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bioRxiv - Plant Biology 2023Quote: ... The over-expressed recombinant proteins were then coupled to CNBr-activated Sepharose (Sigma) at a ratio of 2.5 mg protein to 1 ml Sepharose slurry as described by the manufacturers.
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bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35 days ...
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bioRxiv - Microbiology 2020Quote: ... were treated with or without 25-100 U/mL of human recombinant IFN-α (Sigma-Aldrich) for 4-12 hours after which total RNA was isolated and subjected to qRT-PCR in order to measure expression of ISGs.
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bioRxiv - Microbiology 2019Quote: ... EVs were incubated with Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) at different final concentration (0 to 10 µg/ml) ...
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bioRxiv - Microbiology 2021Quote: For expression of C13/N15 labeled recombinant human wildtype-MIF BL21-(DE3)-Rosetta-pLysS bacteria (Novagen) were transformed with pET11a-MIF and spread on LB-Agar plates containing 50 µg/mL carbenicillin and 34 µg/mL chloramphenicol ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µg/ml BSA fraction V and 10 µg/ml human recombinant in sulin (both Sigma)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human EGF (Cat# E9644) and Glucose Quantification Kit (Cat# GAGO-20) purchased from Sigma-Aldrich; Cisplatin (Cat# 232120 ...
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bioRxiv - Microbiology 2019Quote: ... Glutamax and Pen/Strep and with 100 U/mL recombinant human IL-2 (Roche; Sigma # 10799068001). For the analysis of reverse transcription products ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35±3 days25 ...
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bioRxiv - Physiology 2023Quote: ... and recombinant human interleukin-1β (IL-β) were obtained from Millipore Sigma (St Louis, MO, USA). Thapsigargin (TG ...
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bioRxiv - Molecular Biology 2023Quote: ... TGFB1 signaling was induced with 5 ng/ml final concentration of human recombinant TGFB1 (Sigma-Aldrich) for 4 h.
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bioRxiv - Cancer Biology 2021Quote: ... and bounded proteins were eluted with NETN buffer containing 2 mg/ml biotin (Sigma-Aldrich) for 1 h twice at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... GST and GST-LegK2 proteins were labelled with an anti-GST antibody from rabbit (A7340; Sigma) and detected with an anti-rabbit secondary antibody from goat conjugated with the fluorochrome Alexa Fluor 488 (A11034 ...
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bioRxiv - Bioengineering 2020Quote: ... or human hemoglobin (all proteins were purchased from Sigma). Protein nanorods were prepared using chemically modified human serum albumin.
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bioRxiv - Evolutionary Biology 2022Quote: ... The recombinant protein was then concentrated using centrifugal filter units (Amicon Ultra-4, Millipore) and dialyzed using Slide-A-Lyzer dialysis cassette into storage buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... and 500 U/mL ESGRO recombinant mouse Leukemia Inhibitory Factor (LIF) protein (Sigma, ESG1107) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Purified recombinant S proteins were concentrated using Amicon filtration devices (EMD Millipore, Billerica, MA), buffer-exchanged into PBS and analyzed by SDS-PAGE and Western blotting ...
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bioRxiv - Cell Biology 2021Quote: ... The recombinant protein was purified by affinity chromatography using anti-FLAG affinity resin (Sigma). The purified protein was precipitated by 20% trichloroacetic acid ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: We concentrated recombinant EGFP fusion proteins using Amicon Ultra centrifugal filters (10K MWCO, Millipore) to an appropriate protein concentration in 250 mM NaCl salt buffer ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were concentrated using an Amicon Ultra (10K; Millipore, Billerica, MA, USA).
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bioRxiv - Microbiology 2024Quote: Recombinant ϕLf-UK PSB15 protein was expressed in B834 (DE3) pLysS cells (EMD Millipore) by transforming pR-PSB15 ...
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bioRxiv - Cancer Biology 2019Quote: ... Biotin-alkyne (Sigma),Streptavidin (Sepharose Bead Conjugate ...
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bioRxiv - Biochemistry 2020Quote: ... NHS-biotin (Sigma) was dissolved in DMSO and added to the concentrated protein at a molar ratio of 10:1 such that the final DMSO concentration did not exceed 5% ...
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bioRxiv - Neuroscience 2020Quote: ... Biotin (40μM, Sigma) was added at t=0 ...
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bioRxiv - Genetics 2022Quote: ... biotin (Sigma, B4501) was added into the medium to the final concentration of 500 μM ...
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bioRxiv - Cell Biology 2021Quote: ... Biotin-phenol (Sigma).
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Stromal transdifferentiation drives lymph node lipomatosis and induces extensive vascular remodelingbioRxiv - Pathology 2022Quote: ... 0.0033% Biotin (Sigma), in the case of biotin-streptavidin amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotin (Sigma, B4639) was dissolved in 60 mM NaOH and stored at -20 °C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... and Biotin (Sigma, B4639 ...
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bioRxiv - Immunology 2023Quote: ... biotin (Sigma-Aldrich) was added to a final concentration of 500 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.001% Biotin (Sigma), 10000 U/mL penicillin/streptomycin solution and kept at 26 °C [97] ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin (Sigma-Aldrich; #B4501 ...
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with HKSdaFREE and HKGE (AvantBio) and 5 ng/mL human recombinant animal-free KGF (Sigma-Aldrich), 1.64 mM CaCl2 and 50 ug/mL ascorbic acid ...
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bioRxiv - Bioengineering 2019Quote: ... Recombinant human BMP-2 (Pfizer) was reconstituted in a 0.1% solution of rat serum albumin (Sigma-Aldrich) and 4 mM HCl and mixed with alginate ...
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bioRxiv - Systems Biology 2023Quote: ... CD8+ T-cell cultures were supplemented with 120 IU/ml human recombinant IL-2 (Sigma Aldrich, USA). T-cells were aliquoted into five samples with 1.5 x 106 cells in each condition to obtain cells at five different time points (6 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... 400 µl of solution of recombinant TnC (Supplementary Materials and Methods) or purified human TnC (Merck Millipore) (15 µg/ml in 0.01% PBS-Tween ...
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bioRxiv - Neuroscience 2024Quote: The inhibitory activities of 5–7 and all the reference compounds were determined using modified Ellman’s method [25,42,43] against human recombinant AChE (hAChE, E.C. 3.1.1.7, purchased from Sigma-Aldrich, Prague, Czech Republic) and human plasmatic BChE (hBChE ...
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bioRxiv - Microbiology 2021Quote: ... GGG-azide labelled proteins were concentrated to 25 μM on a 30 kDa Amicon Tube (Merck Millipore) in Tris/NaCl buffer and next labelled with 100 μM DBCO-Cy5 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Cancer Biology 2020Quote: ... and purified human GST protein (5 mg/ml) (Sigma-Aldrich) were incubated for 30min with biotinylated probes (100 pM ...
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bioRxiv - Cancer Biology 2019Quote: ... Murine mCherry-G166 GSCs glioma cells or human G166 GSCs labelled with a Red Fluorescent Cell kit PKH26 (Sigma-Aldrich) were injected into NOD SCID mice ...
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bioRxiv - Genetics 2023Quote: ... Then cells were resuspended in the BD-FACS staining buffer and labelled through anti-human TRA-1-60 antibody (Millipore) and anti-mouse IgM control ...
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bioRxiv - Plant Biology 2019Quote: ... The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore). Protein yield was estimated by measuring absorbance at 595 nm using a spectrophotometer (UV-2600 ...