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Citations for Millipore Sigma :
3401 - 3450 of 10000+ citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and Atto 647N-labeled 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (Atto 647N DPPE, ≤90.0%) were purchased from Sigma-Aldrich. Gibco™ Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... nucleation mix was prepared containing desired concentration of αβ-tubulin (3.5-21 μM) purified from bovine brain with 5% Cy5-labeled tubulin along with 1mg/ml BSA (A7906, Sigma) in assay buffer ...
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bioRxiv - Immunology 2021Quote: ... Labeled antigens were sterilized by filtering through 0.22 µm pore size Spin-X centrifuge tube filters (CLS8160, Millipore Sigma) and stored at 4°C until use ...
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bioRxiv - Plant Biology 2019Quote: ... Labeled standards for indole-3-acetic acid and N-(3-indolylacetyl)-DL-aspartic acid were obtained from Sigma-Aldrich. Calibration curves were linear (r values = >0.99 ...
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bioRxiv - Neuroscience 2019Quote: ... Amplification and label probe binding was performed after further washes with wash buffer A with the amplification reaction mixture containing Phi29 polymerase and the fluorophore-labeled detection oligo prepared according to the manufacturer’s recommendations (Duolink Detection reagents Red, Sigma) in a prewarmed humidified chamber at 37 °C for 100 min ...
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bioRxiv - Genetics 2020Quote: ... male and female WT and Becn1F121A mice were starved overnight before the intraperitoneal (i.p.) injection of 10mmol/kg of 15N-labeled ammonium chloride (98% enriched in 15N, Sigma) dissolved in water ...
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bioRxiv - Neuroscience 2021Quote: ... Thirty micrometer thick hippocampal sections were prepared as described and labeled with Homer1 (rabbit, 1:1000, Millipore, Cat#: ABN37) and purified HA-647 (mouse ...
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bioRxiv - Biophysics 2020Quote: ... 100 μl of biotin-labeled BSA dissolved in buffer A at 1 mg ml−1 (A8549-10MG, Sigma-Aldrich) was flushed through the channel five times and incubated for 5 min ...
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bioRxiv - Bioengineering 2020Quote: Actin and collagen structures in formalin-fixed whole-mount samples were labeled with phalloidin-Atto 488 (1:200, Sigma) and CNA35-OG488 (1 µM ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then labeled with a mouse monoclonal antibody to complement components C3b and iC3b (Clone 3E7; Sigma-Aldrich) diluted 1:100 in blocking solution at 4°C overnight on a rocker ...
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bioRxiv - Microbiology 2021Quote: Adult female worms were incubated with 5 μM of 5’ cy3-labeled Bma-lad-2 siRNA 1 (Sigma Aldrich) for 24 hrs to evaluate uptake of siRNA into intestinal tract epithelial cells ...
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bioRxiv - Cell Biology 2021Quote: ... TMT labeled samples for a given experiment were then pooled and acidified with 5% formic acid (Sigma, v/v). Samples were concentrated using a SpeedVac and resuspended in buffer A (95% water ...
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bioRxiv - Cell Biology 2021Quote: ... Molecular sizes were confirmed using DIG-labeled DNA Molecular Weight Marker II (λDNA with Hind III digestion) (Sigma; #11218590910). Genotyping PCR and DNA sequencing analysis (Figure S1 ...
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bioRxiv - Biophysics 2021Quote: Assembled actin filaments were stabilized and fluorescently labeled via the addition of 4 µM fluorescein-isothiocyanate phalloidin (Sigma Aldrich). Following a 10-minute incubation ...
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bioRxiv - Bioengineering 2020Quote: ... NPs were synthesized by utilizing copolymers fluorescently-labeled by the conjugation of fluorescein isothiocyanate (FITC, green fluorescence, Sigma-Aldrich) or rhodamine isothiocyanate (RITC ...
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bioRxiv - Cell Biology 2022Quote: ... v/v) solution (0.5 ml) containing the following internal standards: C13 and N15 labeled amino acids standard mix (Sigma) was added to the tubes followed by centrifugation ...
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bioRxiv - Neuroscience 2022Quote: ... v/v) solution (0.5 mL) containing internal following standards: 13C- and 15N-labeled amino acids standard mix (Sigma-Aldrich) was added to the tubes followed by centrifugation ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was hybridized with a digoxigenin-labeled DNA probe generated with a PCR DIG Probe Synthesis Kit (Sigma). Hybridization with a 5’ probe produced an 8.1 Kb band from the WT and a 5.1 Kb band from the targeted locus ...
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bioRxiv - Microbiology 2022Quote: ... Biotin labeled RNA probes were generated by adding 1.25 μl of 10 mM biotin-16-UTP (Millipore Sigma 11388908910) to the in vitro transcription reactions ...
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bioRxiv - Microbiology 2021Quote: ... YFP-dsRNA was labeled using the fluorescein RNA Labeling Mix Kit following the manufacturer’s instructions (Sigma, St. Louis, MO). For confocal microscopy examination of fluorescent dsRNA uptake by fungal mycelium ...
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bioRxiv - Biochemistry 2021Quote: ... Culture media were supplemented with natural isotope distribution (12C) or completely 13C-labeled oleic acid (12C: O1383, Sigma Aldrich, Taufkirchen ...
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bioRxiv - Genetics 2022Quote: The labeled cell pellets were thawed on ice and lysed in 500 μL 2X RIPA buffer (Millipore 20-188) containing 1X HALT protease inhibitor (Thermo 78430 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... then orally gavaged with 440 mg/kg body weight of FITC-labeled dextran (FD4) (Sigma, St. Louis, MO, USA). The mice were sacrificed 4 hours later ...
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bioRxiv - Cancer Biology 2022Quote: ... asynchronously growing subconfluent cells were labeled with 30 μM thymidine analogue 5-chloro-2’-deoxyuridine (CIdU) (#C6891, Sigma-Aldrich) for 20 minutes ...
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bioRxiv - Zoology 2019Quote: ... two containing 160 nM cortisol (10% 1,2,6,7-3H-cortisol, Perkin Elmer, Waltham, MA, and 90% non-labeled cortisol, C-106, Sigma-Aldrich) to measure total binding ...
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bioRxiv - Neuroscience 2019Quote: ... Excess dye was then removed by buffer-exchanging the labeled solution into 20 mM Tris (pH 7.4) and 50 mM NaCl buffer using Amicon concentrators (Millipore) and then passed over two coupled HiTrap Desalting Columns (GE Life Sciences).
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bioRxiv - Immunology 2019Quote: Labeled samples were diluted 1:100–1:300 in HEPES DTT buffer (100 mM HEPES, 200 mM DTT, Sigma) and were stained with 0.5 μM of YOYO-1 (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... a 15 μM mixture of 20% pyrene-labeled Mg-ATP-actin monomer with 100X anti-foam 204 (0.005%; Sigma) was placed in the upper well of a 96 well non-binding black plate ...
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bioRxiv - Biophysics 2019Quote: ... coated again with Alexa-488 labeled fibrinogen solution (10 μl of a 50 μg/ml solution in PBS, Sigma) for twenty minutes and stored at 4 °C after another three washing steps ...
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bioRxiv - Biophysics 2020Quote: ... MTSL-labeled GLIC was concentrated using Amicon Ultra-15 (100 kDa molecular weight cut off) concentrator tubes (EMD Millipore) and centrifuged at 90,000 rpm in TLA-120.2 rotor (Beckman Coulter ...
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bioRxiv - Physiology 2021Quote: ... 100 µl homogenate were added to 400 µl of ice-cold methanol/water 4:1 (v/v) / 0.2% formic acid and isotope-labeled internal standards (10 µl 2 µM 13C3,15N-3-hydroxykynurenine (Sigma-Aldrich) and 10 µl 2 µM D5-kynurenic acid (Eurisotop)) ...
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bioRxiv - Immunology 2020Quote: ... labeled with CFSE (COMPANY) and cultured from 4 days with a combination of LPS (25 μg/ml, Sigma-Aldrich), IL-4 (25 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... spirochetes were incubated for 60 min with goat anti-mouse gold-labeled antibody at 1:30 dilution (G7652; Sigma). The grid was further washed ...
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bioRxiv - Microbiology 2022Quote: ... incubated 1 hour with primary mouse monoclonal antibody anti-HA tag (G036, Abm Inc.) diluted 1:10,000 and then probed with horseradish peroxidase-labeled anti-mouse immunoglobulin (A9044, Sigma), diluted 1:15,000 ...
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bioRxiv - Neuroscience 2023Quote: ... CN neurons were labeled with guinea pig anti-NeuN primary antibody (1:500, abN90, Millipore, Burlington, MA, United States) and rabbit anti-GFP primary antibody (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... Cellular cortical actin and nuclei were labeled for 30 min with fluorescein isothiocyanate (FITC)-phalloidin (2 μg/ml) (Sigma) and 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Developmental Biology 2024Quote: ... exponentially growing cells were pulse-labeled for 20 minutes with 25 µM 5-iodo-2-deoxyuridine (I7125, Sigma-Aldrich), followed by a second 20-minute pulse with 250 µM 5-chloro-2-deoxyuridine (C6891 ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with u-[2H,13C,15N] labeled algal lysates (2 g/L of culture unless specified otherwise; Sigma-Aldrich) and 2H,13C D-glucose as an additional carbon and energy source ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were labeled by incubation in PBS containing 4′,6-diamidino-2-phenylindole (DAPI) (0.1 μg/ml) (Sigma-Aldrich), and samples were mounted in ProLong Gold Antifade Mountant (Thermo Fisher Scientific).
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bioRxiv - Physiology 2023Quote: ... Muscle cells expressing sarcolipin or oxidative fibers were labeled with a rabbit polyclonal anti-sarcolipin Ab (Millipore Sigma, ABT13) or mouse monoclonal Ab to SDHB (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... Grids were blocked with 1% BSA / 1% gelatin / 0.01% Tween20 in PBS for 10 min then labeled with primary antibody (anti-fibrinogen Millipore--Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Grids were blocked with 1% BSA / 1% gelatin / 0.01% Tween20 in PBS for 10 min then labeled with primary antibody (anti-fibrinogen Millipore--Sigma, Marlborough ...
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bioRxiv - Bioengineering 2022Quote: ... PLL labeled with fluorescein 5-isothiocyanate (PLLFITC) and 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester (Rhod) were purchased from Sigma.
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bioRxiv - Biochemistry 2023Quote: ... were incubated with 100 nM y-32P labeled RNA in the presence of 2.5 ng/µL polyU competitor (poly-uridine ssRNA, Sigma) for 20 min at room temperature in RNAse-free EMSA-buffer (50 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... and ciliated cells were stained using a Cy3-labeled anti-β-tubulin monoclonal antibody (1:300; Sigma-Aldrich, USA). Alexa Fluor® 488-labeled goat-anti-mouse IgG (H+L ...
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bioRxiv - Biochemistry 2023Quote: ... Kinase-treated cells were then labeled with a 1:1000 dilution of biotinylated 4G10 Platinum anti-phosphotyrosine antibody (Millipore) for an hour on ice and washed with PBS containing 0.1% BSA and 2 mM EDTA (isolation buffer) ...
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bioRxiv - Cell Biology 2023Quote: ... F-actin was selectively labeled with fluorescent phalloidin (Phalloidin-ATTO 647N conjugate, Millipore-Sigma, Cat#65906; 10 U/mL). Cell chromatin was labeled with 1:1000 Hoechst solution (Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... F-actin was selectively labeled with fluorescent phalloidin (Phalloidin-ATTO 647N conjugate, Millipore-Sigma, Cat#65906; 10 U/mL). Cell chromatin was labeled with 1:1000 Hoechst solution (Tocris ...
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bioRxiv - Developmental Biology 2023Quote: ... DKO and control embryos were sectioned at 10 μm for in situ hybridization using DIG-labeled RNA probes (Sigma). DNA templates for making RNA probes were generated using PCR with primers (Suppl ...
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bioRxiv - Microbiology 2023Quote: ... 3D dSTORM imaging of click-labeled DNA (Figure 1) was performed in PBS containing 100 mM MEA (Sigma Aldrich), adjusted to pH 8.5 using NaOH.