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Citations for Millipore Sigma :
3401 - 3450 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Membrane were then blocked for 1 hr with 5% milk in Tris-buffered saline plus 0.02% Tween20 (Sigma-Aldrich). Membranes were incubated with the primary antibodies O/N at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was washed once with TBST and incubated with antibodies against FLAG (1:5000, Sigma, Cat no.# F3165), V5 (1:5000 ...
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Hypoxia-mediated suppression of pyruvate carboxylase drives tumor microenvironment immunosuppressionbioRxiv - Cancer Biology 2022Quote: ... the membranes were incubated overnight at 4°C with anti-PC antibody (1:500, HPA043922, Millipore-Sigma, Darmstadt, Germany), anti-beta tubulin antibody (1:2000 ...
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Hypoxia-mediated suppression of pyruvate carboxylase drives tumor microenvironment immunosuppressionbioRxiv - Cancer Biology 2022Quote: ... the membranes were incubated overnight at 4°C with anti-PC antibody (1:500, HPA043922, Millipore-Sigma, Darmstadt, Germany), anti-beta tubulin antibody (1:2000 ...
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bioRxiv - Developmental Biology 2020Quote: ... membrane was incubated for 1 h at room temperature with anti-rabbit IgG horseradish peroxidase (HRP) conjugate (Sigma-Aldrich) at a concentration of 1:1000 in 5% (w/v ...
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bioRxiv - Cell Biology 2019Quote: Proteins were separated using SDS-PAGE gel composed of bisacrylamide (37:5:1) and blotted onto a polyvinylidene difuoride membrane (0.45-µm pore size; Millipore). Nonspecifc sites were blocked in skimmed milk in the presence of 0.1% Tween 20 ...
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bioRxiv - Neuroscience 2019Quote: ... transferred to a nitrocellulose or PVDF membrane and blocked for 1 hour in 5% bovine serum albumin (Sigma, A7906) in TBS-T ...
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bioRxiv - Physiology 2020Quote: ... Membranes were probed overnight at 4°C with anti-phosphotyrosine antibody (1/1000, 05-321, clone 4G10, Merck Millipore) in blocking solution ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... The polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with appropriate antibodies: anti-Arp (dilution 1:1,000; Sigma); anti-FHOD1 (dilution 1:1,000 ...
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated for 30 minutes at room temperature with anti β-actin (1:10000, A3854 Sigma-Aldrich), wich is used as a normalizer ...
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bioRxiv - Molecular Biology 2019Quote: ... supernatant containing solubilized IBs was diluted 1:4 in lysis buffer containing 8M urea and filtered through a 0.22 μm membrane (Millipore). Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Microbiology 2020Quote: ... parvula at 105 cells mL−1 were placed into 50 mL conical tubes separated by a 0.22 μm membrane (Steriflip-GP, Millipore). Three conditions were tested ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in Odyssey blocking buffer TBS (Licor) or 5% non-fat milk or 1% BSA (Sigma, A7906) for 1 hour before over-night incubation at 4°C with primary antibodies ...
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bioRxiv - Physiology 2023Quote: ... membranes were exposed to overnight at 4°C with 1:15,000 anti-β-actin (Sigma Aldrich; Catalogue number: A5441) and for 1h at RT goat anti-mouse IRDye 680LT (LI-COR ...
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bioRxiv - Microbiology 2022Quote: ... The total membranes were solubilized with a buffer containing 1×PBS (pH 7.4) and 0.5% N-lauroylsarcosine sodium (Sigma-Aldrich) for 1 hr at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated with goat anti-rabbit AP-conjugated antibodies (1:5000 in PBS, Sigma-Aldrich, Missouri, USA), followed by four washes with PBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... Next the membrane was blocked with PBS-milk 5% and incubated with primary antibodies s9.6 1:500 (Millipore, MABE1095) and dsDNA 1:3000 (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... was cultured in 1 mL collagen on top of a 0.4 µm filter Transwell PFTE membrane insert (PICMORG50, Millipore) in 6 well plates ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were analyzed on an SDS–polyacrylamide (37.5:1) gel electrophoresis and blotted on 0.45 µm pore PolyVinyliDenFluoride (PVDF) membranes (Immobilon-P from Sigma Aldrich). Membranes were blocked 2h with 1X Tris-buffered saline (TBS ...
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Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic WoundsbioRxiv - Microbiology 2023Quote: PAW-induced outer membrane permeability was measured based on fluorescent dye N-phenyl-1-naphthylamine (NPN; Sigma-Aldrich, Australia) uptake [22] ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were blocked with 5% BSA in TSMT (20 mM Tris; 150 mM NaCl, Merck; 1 mM CaCl2, Sigma; 2 mM MgCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were incubated overnight at 4°C in the following primary antibodies: Fas (EMD Millipore 05-351, 1:500), Phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was incubated overnight at +4°C with the following primary antibodies: anti-TGFBR2 (1:1000, SAB5700739, Sigma), anti-TGFB1 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with an α-FLAG antibody (Sigma; 1:1,000 in 1x TBS-T containing 3% BSA) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... PVDF membrane was blocked by 30 min incubation in TBS-T (TBS 10x: 200mM Tris (Sigma, #1185-53-1), 1.37M NaCl (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... The phosphorylated histidine was detected by Western blotting by then incubating the membrane for 60 min with 1:1.000 anti-N1-phosphohistidine antibody (Merck Millipore) in TBS-T followed by a washing step for 30 min with TBS-T and a final 60 min incubation with with 1:10.000 horseradish peroxide-conjugated anti-rabbit IgG (Promega ...
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bioRxiv - Microbiology 2024Quote: ... Blocked membrane was incubated overnight at 4 °C with primary antibody anti-FLAG (F3165, Sigma-Aldrich, 1:2000 dilution), washed 3 times in TBS with 1% Tween 20 (TBS-T) ...
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bioRxiv - Cell Biology 2020Quote: ... RNA immunoprecipitation was conducted using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma 17-700) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The protein content was measured using the BCA Kit (Bicinchoninic Acid, Sigma-Aldrich, St Louis, MO, USA) and bovine serum albumin (BSA ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... was washed 3 times with PBS and processed using the CelLytic MEM protein extraction kit from Sigma. Briefly ...
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bioRxiv - Microbiology 2021Quote: ATPase activity of Wt and mutant HelR proteins was determined using the colorimetric ATPase assay kit (Sigma). The ATP hydrolysis reaction was performed by incubating 0.3 µM HelR with 1 mM ATP (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: The RIP assay was performed using a Magna RIPTM RNA Binding Protein Immunoprecipitation Kit (17-700; Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: The RBD and spike proteins were chemically biotinylated using the EZ-Link NHS-PEG4-Biotin kit (Sigma). For the RBD ...
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bioRxiv - Cancer Biology 2020Quote: RIP assay was performed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma, Burlington, MA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations of wholecell lysates were determined by the QuantiPro™ BCA Assay Kit (Sigma-Aldrich, QPBCA). The proteins were then electrophoresed in SDS-PAGE gels to separate by molecular weight and transferred to polyvinylidene difluoride membranes (Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... Interactions between CB1 and cytoskeleton proteins were detected using the Duolink in situ PLA detection Kit (Sigma) following the instructions of the supplier ...
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bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation (RIP) was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Burlington, MA). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial and cytoplasmic protein was isolated using the mitochondrial/cytoplasmic fractionation kit from Millipore Sigma (cat.#: MIT1000). Primary antibodies used include the following ...
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bioRxiv - Cancer Biology 2023Quote: ... The ECM fraction was isolated from the frozen tissues using the Compartment Protein Extraction Kit (Millipore, #2145) as per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were extracted from residue debris pellets and quantified using a BCA kit (Sigma-Aldrich, Taufkirchen, Germany). Metabolite profiling was performed using a Sciex ExionLC AD coupled to a Sciex ZenoTOF 7600 under the control of Sciex OS 3.0 (Sciex ...
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bioRxiv - Microbiology 2023Quote: RNA-binding protein immunoprecipitation (RIP) was conducted using the Magna RIP kit (catalog no. 17-700; Millipore) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... the lysates were separated on SDS-polyacrylamide gels and transferred to polyvinylidene fluoride membranes (Immobilon P membrane, Millipore). The membranes were blocked at room temperature for 30 min in TBST containing 5% skimmed milk (Nacalai Tesque ...
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bioRxiv - Plant Biology 2021Quote: ... it was filtered through a 0.22 μm filter membrane (Durapore GV membrane filters, 0.22 μm, Millipore, Molsheim, France). A HisTrap HP column (5 ml ...
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bioRxiv - Microbiology 2020Quote: ... tumefaciens cell cultivated in induction medium overnight were filtered onto a 0.45 µm cellulose acetate membrane (0.45 µm MCE Membrane, MF-Millipore) and incubated on induction medium agar plates for 2 days ...
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bioRxiv - Immunology 2021Quote: ... the supernatant was cleaned by filtration on a 0.45 µm pore-size membrane then on a 0.22 µm membrane (Millipore). The total volume was aliquoted ...
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bioRxiv - Microbiology 2020Quote: ... The growth analysis plate was covered with a breathable sealing membrane (Breathe-Easy® sealing membrane, Sigma, Australia), and incubated at 37 °C for 15 hours with shaking (300 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... and the slices were placed on Millicell membranes (3–4 slices per membrane, 0,4 µm Millicell, Merck Millipore). Slices were cultured in DMEM/F-12 with GlutaMax ...
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bioRxiv - Plant Biology 2023Quote: ... After size fractionation by electrophoresis the gels were blotted onto membrane (Blotting-Nylon 66 membranes, Sigma-Aldrich # 15356), followed by UV fixation ...
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bioRxiv - Cell Biology 2023Quote: CR expanded HBE cells were seeded on collagen I coated 24-well Transwell membranes (Corning, USA; 6.5mm 0.4µm pore polyester membrane Sigma CLS3470) at a density of 1.5×105 cells/insert ...