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Citations for Millipore Sigma :
3351 - 3400 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... 15 μL diluted proteins were mixed with 1 μL 100x Sypro Orange (Sigma-Aldrich) and loaded in LightCycler 480 Multiwell Plate 96 (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Target proteins were bound to 1 mL Ni-NTA His-Bind resin (Millipore Sigma) in gravity columns ...
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bioRxiv - Neuroscience 2021Quote: ... and those directed against the following proteins: Actin (rabbit, Sigma, A2066, WB 1 : 2000), GFAP (mouse ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were digested on-bead with 1 µg of trypsin (Sigma Aldrich, Cat# 6567) in 50 µL ABC buffer with rotation at 37℃ overnight ...
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bioRxiv - Biophysics 2022Quote: ... Protein expressions were induced by 1 mM isopropyl β-D-thiogalactopyranoside (IPTG, Sigma-Aldrich) overnight at 25°C ...
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bioRxiv - Cancer Biology 2022Quote: ... PBS and 1 μg/mL Human Plasma Fibronectin Purified Protein (Sigma-Aldrich, Cat. #: FC010). 1N NaOH was added to achieve a neutral ∼pH and a thin base layer of 100 μL volume was added to the well of a 48-well tissue culture plate precoated with 1% agarose ...
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bioRxiv - Cancer Biology 2022Quote: ... [1] using the protein A sepharose beads (GE Cat # 17-0780-01; Millipore-Sigma). The concentrated BCG-CM (containing 1.0 mg protein in the lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... [1] using the protein A sepharose beads (GE Cat # 17-0780-01; Millipore-Sigma). The concentrated BCG-CM (containing 1.0 mg protein in the lysis buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were initially cross-linked to DNA with 1% formaldehyde (Sigma, Cat. No F8775) for 15 minutes in 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were stained using primary antibodies against β-actin (1:10,000, AC-15, Sigma), strep II-tag (1:1,000 ...
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bioRxiv - Developmental Biology 2019Quote: ... Primary antibodies used included anti-rabbit glial fibrillary acidic protein (GFAP, Sigma, 1:400), mouse anti-Zrf1 (Gfap ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole cellular protein was extracted with RIPA buffer containing 1 mM Na3VO4 (Sigma-Aldrich) and protease inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2020Quote: ... The protein was concentrated to 5 mg·ml−1 in an Amicon ultracentrifugation device (Millipore) with 10 kDa cutoff and directly used for further studies or supplemented with 40% glycerol ...
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bioRxiv - Neuroscience 2020Quote: Mouse anti-Myelin Basic Protein (MBP-SMI-99, Millipore, reference NE1019, 1/1000 dilution), rat anti-Myelin Basic Protein (BIO-RAD ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies used were rabbit anti-prosurfactant protein C (SPC) (Millipore, AB3786, 1:500) and goat anti-IL-33 (R&D ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were initially cross-linked to DNA with 1% formaldehyde (Sigma, Cat. No F8775) for 15 minutes in 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal antibody directed against Purkinje cell protein 4 (PCP4; Sigma, dilution 1:400), rabbit polyclonal antibody raised to Capn15 (Zha ...
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bioRxiv - Neuroscience 2020Quote: ... Synaptic proteins were detected mouse anti-PSD95 (1:500, MAB1598, Millipore, Burlington, MA, U.S.A.), guinea pig anti-VGLUT1 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Soluble proteins were then incubated with 1 μg of anti-FLAG antibody (F1804, Sigma) or 1 μg of mouse IgG (sc-2025 ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed with 50 μL of 1 × BugBuster Protein Extraction Reagent (Sigma). Lysates clarified at 21 ...
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bioRxiv - Plant Biology 2022Quote: ... The total ubiquitinated proteins were detected by anti-FLAG (F-3165, 1:5,000, Sigma) and α-MYC (ab32 ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected with an anti-FLAG antibody (1:500, Sigma F1804). Samples were also stained with DAPI (300 nM ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested overnight at 37C with trypsin (1:50 µg/µg) (Sigma-Aldrich) and lysyl endopeptidase (1:75 µg/µg ...
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bioRxiv - Neuroscience 2023Quote: ... and glial fibrillary acidic protein (mouse anti-GFAP, 1:400, Sigma-Aldrich, cat. #: G3893), and cell nuclei were counterstained using Hoechst (10 µg/mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM TCEP and mixed with 1:5000 SYPRO Orange Protein Gel Stain (Sigma). 50 μL of assay mixture was dispensed per well in a white bottom qPCR plate and each condition was performed in technical triplicate ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with antibodies against OL markers proteins including Olig2 (MABN50, 1:500 Millipore, CA ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were probed with 1:2000 chicken anti-LDL primary antibody (#GW20089F, Sigma-Aldrich) diluted in PBS-T overnight at 4°C and detected with 1:2000 HRP-conjugated goat anti-chicken secondary antibody (#A16054 ...
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bioRxiv - Biochemistry 2023Quote: ... Expression of chaperone protein was induced with 1 μg/ml tetracycline (Sigma-Aldrich, T7660).
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bioRxiv - Cell Biology 2024Quote: ... The expression of proteins was induced with tetracyclin (1 µg/mL; Sigma-Aldrich, T7660). Cell were treated with actinomycin D (10 ng/mL ...
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bioRxiv - Neuroscience 2023Quote: ... PLP (Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1 MAB388 Sigma-Aldrich, 1:200), MHCII (Dako 1:50) ...
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bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... a primary antibody against the HCoV-OC43 N protein (Millipore #MAB9013; diluted 1:500) was used ...
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bioRxiv - Immunology 2024Quote: ... The first two immunizations included 100µg/100µl OVA protein (Sigma Aldrich, 9006-59-1) in complete Freund’s adjuvant (CFA ...
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bioRxiv - Developmental Biology 2022Quote: ... using 1:4 phosphate buffer from the Total SOD kit (Sigma-Aldrich, kit 19160) for SOD activity ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 50 hpf zebrafish embryos using the lysis buffer from the calpain 1/2 activity kit (InnoZyme™ Calpain 1/2 Activity Assay Kit CBA054 purchased from Merck Millipore). Protein concentration was adjusted to 2 mg/mL and calpain enzymatic activity was measured in microtubes following the manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µg of each fraction was loaded in 10% SDS-PAGE gel and transferred to PVDF membrane (Millipore Sigma). Membranes were incubated in blocking solution for 1 h at RT (3% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with 5% skim milk and probed with primary antibodies: anti-FLAG (1:1000) (Sigma-Aldrich, F1804), anti-MRG-1 (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were separated by 8% or 10% Tris-Glycine-SDS page gels for 1 h and transferred on PVDF membrane (0.45 um, Millipore). Then blocked the membrane with 5% skimmed milk in TBS-T buffer (20 mM Tris-HCl ...
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bioRxiv - Systems Biology 2020Quote: ... Cultures were grown to mid-log and 1 mL was transferred to a 0.45 μm PVDF membrane filter paper (Millipore), fixed atop a vacuum manifold ...
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bioRxiv - Microbiology 2019Quote: ... the membrane was incubated in a 1:1000 dilution of ANTI-FLAG® M2-Alkaline Phosphatase antibody (Sigma Aldrich) in TBS-T ...
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bioRxiv - Plant Biology 2021Quote: ... The membranes were probed for 16 h with Concanavalin A conjugated with horseradish peroxidase (1 mg/mL; Sigma-Aldrich), washed for 45 min with PBS ...
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bioRxiv - Immunology 2021Quote: ... 4 million cells per condition were washed once with ice-cold saline solution (0.9% NaCl solution filtered through a 0.22 μm membrane filter) and resuspended in 1 ml of 50% (vol/vol) methanol (Sigma) (0.22 μm-filtered ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized proteins were separated from the insoluble fraction by centrifugation at 60,000 g for 1 hr at 4° C and the supernatant was filtered through a 0.22-μm polystyrene membrane (Millipore). GFP nanobody was coupled to CNBr-activated Sepharose Fast Flow beads (GE healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized proteins were separated from the insoluble fraction by centrifugation at 60,000 x g at 4° C for 1 hr and the supernatant was filtered through a 0.22-μm polystyrene membrane (Millipore). 2 mL GFP nanobody resin was incubated with the sample with agitation at 4 °C for 1 hr ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred to nitrocellulose membrane and probed with primary antibodies against HA (HA-7 mouse monoclonal, 1:2000 dilution, Sigma; or rabbit polyclonal ...
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bioRxiv - Biochemistry 2021Quote: ... The membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma) at a 1:10,000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant E1E2 was extracted from cell membranes using 1% NP-9 and purified via sequential Fractogel EMD TMAE (Millipore), Fractogel EMD SO3− (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... supernatant containing solubilized IBs was diluted 1:4 in lysis buffer containing 8M urea and filtered through a 0.22 μm membrane (Millipore). Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated in blocking buffer for 1 hour at 25°C (TBST with 3% BSA; Sigma) before being probed for LapA or MapA containing an HA tag with an anti-HA antibody (Biolegend ...