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Citations for Millipore Sigma :
3251 - 3300 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then incubated with anti-FLAG M2 primary antibody (1:500 dilution; Millipore Sigma; Cat #: MF1804) in PBS/0.1%Tween20/5% Blotting-Grade Blocker overnight ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed and blocked as per western blotting protocol and probed with 1:1500 BG4 (Merck Millipore), 1:1500 DYKDDDK tag (anti-flag ...
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bioRxiv - Developmental Biology 2020Quote: ... the membrane was washed and incubated with the primary antibodies against ß-actin antibody (1:10,000, Sigma #A5441) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were incubated with HRP-linked secondary antibodies anti-mouse IgG (1:5000, A6782, Sigma-Aldrich, MO, USA) and anti-rabbit IgG (1:5000 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were probed at 4°C with primary antibodies: polyclonal anti-UCP1 (1:500, Sigma-Aldrich cat#U6382), and anti-actin (1:10000 ...
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bioRxiv - Molecular Biology 2019Quote: ... transferred to PVDF membranes and probed with the following primary antibodies: anti-FLAG HRP (1:300, Sigma, A8592), anti-GAPDH (1:1200 ...
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bioRxiv - Cancer Biology 2020Quote: ... electrophoretically transferred to PVDF membranes and blocked for 1 hour in 5% BSA and TBST (Sigma G-8898). Antibodies ...
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bioRxiv - Immunology 2021Quote: ... Stripping of the membranes for re-probing has been performed using buffer containing 1% Tween-20 (Sigma P2287), 0.1% SDS (Sigma 71729) ...
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bioRxiv - Cancer Biology 2021Quote: ... Gels were transferred onto PVDF membranes and stained with primary antibodies against CD-9 (1:1000, Millipore #CBL162,), CD-63 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated at 4 °C overnight with primary antibodies against full-length APP (1:1,000, Millipore), APP-CTFs (1:1,000 ...
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bioRxiv - Plant Biology 2021Quote: ... The membranes were then probed with a 1:5000 dilution of Roche anti-GFP (Sigma Aldrich Cat# 11814460001) or a 1:5000 dilution of anti-mouse HRP secondary antibody (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... the membrane was incubated overnight with a 1:2000 dilution of mouse monoclonal anti-FLAG antibody (Sigma-Aldrich) in blocking solution at 4 ºC with mild shaking ...
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bioRxiv - Cell Biology 2020Quote: ... Proteasome complexes were subsequently blotted for 1 h at 200 V on ice onto PVDF membranes (Merck Millipore) and further handled as described above.
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bioRxiv - Neuroscience 2022Quote: ... The membrane was blotted with the following primary antibodies: MAB 2166 (anti-HTT, 1:1000, Millipore Sigma, USA), MW1 (anti-poly-Q ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were incubated with the appropriate secondary antibodies (anti-mouse or anti-rabbit, 1:5000; Sigma, Italy) for 1h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were incubated with the following primary antibodies: anti-PSD-95 clone 6G6-1C9 (1:1000, MAB1596, Millipore), anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were blocked for 1 hour in either 5% milk or 5% Bovine Serum Albumin (BSA) (Sigma, A7906) diluted in PBS/0.2% Tween ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked for 1 h at room temperature in TBS-T (0.1%) - 5% milk (Sigma-Aldrich, Ireland). Primary antibodies were diluted in TBS-T (0.1% ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with one of the following primary antibodies: anti-cyclin G2 (1:500, HPA034684, Sigma); Flag (1:500 ...
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bioRxiv - Genomics 2019Quote: ... We then incubated the membrane with Horse Radish Peroxidase-conjugated secondary antibody (Anti-mouse 1:15,000, A9044, Sigma; Anti-rabbit 1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 30 min at RT with anti-β-actin (1:10’000, A3854 Sigma-Aldrich), which is used as a normalizer.
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bioRxiv - Bioengineering 2019Quote: ... The membrane was stripped and incubated with anti-beta-actin (F5316, 1/2000; Sigma-Aldrich, St. Louis, MO), incubated with HRP-conjugated anti-mouse antibodies (#31430 ...
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bioRxiv - Microbiology 2020Quote: ... After transfer, the membrane was incubated for 1 hour in blocking buffer (PBS, 0.1% Tween 20 (Sigma, 93773), supplemented with 5% milk or 5% BSA according to antibody recommendation) ...
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bioRxiv - Genomics 2020Quote: ... The membrane was washed and incubated in a 1/2500 dilution of the rabbit-HRP secondary antibody (Sigma). After washing ...
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bioRxiv - Neuroscience 2023Quote: ... transferred to polyvinylidene fluoride (PVDF) membranes and probed for eYFP expression (Millipore; polyclonal anti-GFP antibody, 1:1000). Blots were also probed with an alpha-tubulin antibody (Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was then incubated overnight at 4°C with 1:2000 M2 Anti-FLAG antibody (Sigma-Aldrich) in 5% milk-PBST ...
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bioRxiv - Biophysics 2024Quote: ... Next day membranes were incubated with peroxidase conjugated anti-rabbit IgG raised in Goat (Sigma, 1:8000 dil.) and peroxidase conjugated anti-mouse IgG raised in Rabbit (Sigma ...
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bioRxiv - Biophysics 2024Quote: ... and 1 mM NaN3 (pH 6.5) and concentrated using conical spin concentrator (10 kDa membrane cut-off, Millipore) to a solution at 80-100 μM ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were reprobed with the anti-actin 20-33 rabbit polyclonal antibody (1:10,000 dilution; Sigma Aldrich #A5060). For comparison of protease-resistant α-syn banding patterns ...
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bioRxiv - Bioengineering 2023Quote: ... then membranes were incubated overnight at 4 °C with rabbit anti-BCRP (Sigma-Aldrich, cat# ZRB1217, 1:1000) or mouse anti-active β-catenin (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... for the ciliary membrane and a mouse anti-gamma tubulin antibody (clone GTU-88 1:5000, Sigma Aldrich) for the basal body ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples from exponentially growing cells were separated on a SDS–polyacrylamide (37.5:1) gel electrophoresis and blotted on 0.45µm pore PolyVinyliDenFluoride (PVDF) membranes (Immobilon-P from Sigma Aldrich). Membranes were blocked for 2 hours with 1x Tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked for 1 hours using TBS-T (0.1% Tween-20) supplemented by 5% BSA (Sigma), and incubated overnight at 4°C with the following primary antibodies ...
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bioRxiv - Genetics 2022Quote: ... The membrane was then incubated overnight at 4°C with anti-H3 [1:5,000 (07-690, Sigma-Millipore)] ...
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bioRxiv - Genetics 2022Quote: ... The membrane was then incubated overnight at 4°C with anti-H3 [1:5,000 (07-690, Sigma-Millipore)] ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then cut and the upper half was incubated in 1:1000 Cep89 antibody (Sigma, HPA040056), the bottom half in B-actin antibody 1:2000 (CST ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with anti-His primary antibody 1:5000 in 4% milk in PBS-T (Sigma) for 2 hours ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and incubated 1 h at RT with peroxidase-conjugated secondary antibodies (anti-mouse, Sigma-Aldrich Cat# A4416 ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked for 1 hour with 5% bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO) in TBS (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were probed overnight at 4°C with antibodies for SLC35A2 (Sigma Aldrich HPA036087 Rb 1:100, polyclonal) and GAPDH (Abcam Mouse 1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibodies against KRAS4B (WH0003845M1, Sigma Aldrich, 1 μg/ml) or HSC70 (sc-7298 ...
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bioRxiv - Neuroscience 2024Quote: ... nitrocellulose membranes were rapidly rinsed in PBS and subjected to crosslinking in 1% glutaraldehyde (Sigma-Aldrich; Cat #: G5882) in PBS for 15 min with rotation ...
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bioRxiv - Developmental Biology 2024Quote: ... Membrane was then incubated with either a 1:4000 dilution of a GFP specific primary antibody (Sigma; G1544) or a 1:3000 dilution of a RFP specific primary antibody (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: Membranes were developed for 1-5 seconds using an enhanced chemiluminescence reagent (Luminata Forte HRP substrate; Millipore Sigma) in a gel documentation system (ChemiDoc Touch ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked in 5% skim milk for mouse anti-Actin-β (1:10,000, A1978; Sigma-Aldrich Co.), and 5% BSA (Merck ...
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bioRxiv - Immunology 2022Quote: A 96 well ELISA plate was coated with bovine fetuin (5 μg/mL; Sigma) in carbonate buffer overnight ...
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bioRxiv - Immunology 2020Quote: ... followed by the addition of the TMB/E ELISA substrate (Merck Millipore, Billerica, MA). Absorbance was measured as described.
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bioRxiv - Neuroscience 2023Quote: ... IAPP was measured by an enzyme-linked immunosorbent assay (ELISA; Merck Millipore, EZHA-52K).
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bioRxiv - Microbiology 2024Quote: ELISA plates were coated with 1mg⍰mL of chondroitin sulphate A (CSA) (Sigma, C8529) in PBS (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... transferred to nitrocellulose membranes, and immunoblotted with α-Psd1 (1:1000, antibody kind gift from S. Claypool) or α-G6PDH (1:2000, Sigma-Aldrich A9521). Anti-rabbit antibody conjugated to DyLight 800 (1:10000 ...