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Citations for Millipore Sigma :
3401 - 3450 of 10000+ citations for 6 Chloro 5 fluoro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 2 mL of 0.3% agarose in DMEM supplemented with 2% FBS or 3 mL of 0.6 or 1.2 percent microcrystalline cellulose (MCC; Sigma 435244) in serum-free DMEM was added ...
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bioRxiv - Neuroscience 2022Quote: ... On selected sections, immunoreactivities were tested: SATB1 (mouse, 1:500, SantaCruz) and GluR 2/3 (rabbit, 1:500, Millipore) were diluted in 0.1 M PB and incubated overnight at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... washed in PBS and incubated in buffer A (10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Plant Biology 2022Quote: ... After addition of 10μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) solution (5mg/ml, Sigma-Aldrich, USA) the cells were incubated in darkness for another 4 hrs ...
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bioRxiv - Microbiology 2022Quote: The effect of CAPG depletion on viability of KO and siRNA-treated cells was assessed at several timepoints using a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay (11465007001; Sigma-Aldrich) per manufacturer directions ...
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bioRxiv - Neuroscience 2022Quote: Recordings were then performed in oxygenated aCSF superfused at a rate of 2-3 ml/min and supplemented with 100 µM picrotoxin (Alomone Labs) and 0.5-1 µM Strychnine (Sigma). The internal solution was the same as above with 2-4 mM QX314 to block Na+ channels from driving AP firing ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL aliquots were taken from the main culture for chemical fixation with 3% paraformaldehyde (PFA, Sigma-Aldrich, #F8775) at 20 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... The PDMS microstructures were then rinsed with 2:3 HEPES1/ethanol and with ultrapure water (Milli-Q, Merck-MilliPore). PVP (1.3 MDa ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Sigma-Aldrich (St Louis, MO). Phalloidin-iFluor 594 was purchased from Abcam ...
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bioRxiv - Microbiology 2024Quote: ... after which the remaining supernatant (2 x 45ml) was collected and concentrated over a 3 kDa Amicon filter (Millipore). The residue was suspended in a volume of 900 µl ...
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bioRxiv - Neuroscience 2024Quote: ... cells grown in 96-well plates were treated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, Hungary) in a final concentration of 200 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium Bromide (MTT) cell growth kit (CT02, Millipore, MA, USA) was used for measuring cell viability ...
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bioRxiv - Cell Biology 2023Quote: ... 1 3 v/v) at 80°C for 2 h after addition of 50 nmol 5α-Cholestane (Sigma, C8003) as internal standard for neutral sterol analysis ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (60) ...
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bioRxiv - Immunology 2023Quote: ... RIPA 400 mM NaCl, (3) Sarkosyl Buffer (2 mM EDTA, 50 mM Tris-HCl, 0.2% sarkosyl (Lauroylsarcosine sodium salt (Sigma)) ...
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bioRxiv - Microbiology 2023Quote: ... and AP2XII-1-AID-HA /AP2XI-2-mAID-MYC was achieved with 3-indoleacetic acid (IAA, Sigma-Aldrich # 45533). A stock of 500 mM IAA dissolved in 100% EtOH at a ratio of 1:1,000 was used to degrade mAID-tagged proteins to a final concentration of 500 μM ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed in 80% ethanol for 2-3 minutes and differentiated in 0.1% lithium carbonate (Sigma #62470) for 45-60 seconds followed quickly by washes in distilled water to stop the differentiation ...
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bioRxiv - Microbiology 2023Quote: The effect of drug treatments on cell viability was assessed using the MTT [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay (the MTT powder was obtained from Sigma Aldrich). VTN or Huh-7 cells were seeded the day before treatment at a concentration of 7L×L103 cells per well in DMEM supplemented with 10% FBS and 1% Pen/Strep ...
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bioRxiv - Cancer Biology 2022Quote: ... Five thousand cNF-derived primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated opaque 96-well plates in SCM without IBMX with Forskolin and maintained at 37ºC under a 10% CO2 atmosphere for 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: One hundred thousand primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated 12-well plates ...
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bioRxiv - Cancer Biology 2022Quote: ... One hundred thousand primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated 12-well plates in SCM without IBMX ...
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bioRxiv - Biophysics 2022Quote: ... Concentrated Cav1.2(ΔC)/Cavβ3/Cavα2δ-1 sample was immediately incubated with gabapentin (final concentration 2 mg ml-1, 11.7 mM) (Sigma-Aldrich) on ice for four hours prior to cryo-EM sample preparation ...
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bioRxiv - Developmental Biology 2023Quote: ... unlysed embryos were cultured during 2 to 3 hours in 40 µL micro drops of TCM199 (Sigma-Aldrich, M4530) + 10% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5mg/ml of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich®, St Louis, MO, USA) was added and incubated at 37°C for 3h ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (Pizzato Scomazzon et al. ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was mixed at a 3:2 ratio with a crowding buffer [20% dextran (from Leuconostoc spp., Mr 450,000-650,000; Sigma 31392) and 1 mM DTT in 50 mM Tris-HCl pH 7.4] ...
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bioRxiv - Neuroscience 2023Quote: ... in 0.4% Triton X-100 and 2% NHS PBS solution during 3 h and then coverslipped with Mowiol (Sigma). After confocal imaging ...
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bioRxiv - Cell Biology 2023Quote: ... Treatment of mice were performed with a single intraperitoneal injection of either vehicle (corn-oil) or 3 mg/kg 1,4-bis-[2-(3,5,-dichloropyridyloxy)] benzene (TCPOBOP, T1443, Sigma-Aldrich).
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 x in TBS-Tween (0.1%) + 2% BSA before incubation in TRITC conjugated anti-Mouse secondary antibody (Sigma) at 1:1000 concentration for 1 hr in the dark ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 99.7% pure), Bisphenol TMC (Catalog #, 52746-49-3) and 2′,7′-Dichlorodihydrofluorescein diacetate (Catalog #, D6883) were purchased from Millipore Sig ma (MO ...
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bioRxiv - Cell Biology 2021Quote: ... 3 (Sigma) in de-ionised (DI ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... 3 (Sigma) as counterstain.
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bioRxiv - Developmental Biology 2020Quote: ... nuclei were stained with DAPI (2 μg/ml, room temperature, 5 min, Sigma, St. Louis, Missouri, USA).
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bioRxiv - Plant Biology 2022Quote: ... DSF supplementation was performed by adding with 5 μM of cis-11-methyl-2-dodecenoic acid (Sigma) to the media.
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS and human fibroblast growth factor-2 (5 ng/mL, Sigma-Aldrich, St Louis, MO, USA) at 37 °C ...
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bioRxiv - Microbiology 2019Quote: ... yeasts were incubated in 1 mL of 5% 2-mercaptoethanol in SPM buffer (Sorbitol 1.2 M (Sigma), potassium phosphate monobasic 50 mM (Sigma) ...
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bioRxiv - Molecular Biology 2019Quote: Mouse monoclonal α-Flag (M2) and α-tubulin (clone B-5-1-2) were obtained from Sigma. Rabbit polyclonal α-HA (ab9110 ...
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bioRxiv - Physiology 2020Quote: ... 5 mM glucose and 2% BSA for two hours while treated with 1 μM isoproterenol (Sigma-Aldrich), 10 μM forskolin with 0.5 mM IBMX (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... The minimal medium was supplemented with 5 mM 2-(N-morpholino) ethanesulfonic acid (Mes: Sigma Chemical Co.) adjusted to pH 6.4 with NaOH as buffer and 0.5% (w/v ...
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bioRxiv - Microbiology 2021Quote: 20 μg of fraction 19 were reduced with 5 mM Tris(2-carboxyethyl)phosphine (646547, Sigma Aldrich) for 60 min at 65 °C ...
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bioRxiv - Microbiology 2020Quote: ... Continuous culture was typically carried at 2-5%-hematocrit in RPMI-1640 (Sigma Aldrich, Cat. No. R6504) supplemented with HEPES ...
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bioRxiv - Immunology 2022Quote: Total RNA was collected from cultured cells treated with 100 nM 5-aza-2⍰-deoxycytidine (decitabine; Sigma) or splenocytes from a naïve C57BL/6 mouse using NucleoSpin RNA Plus (Takara) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Samples were combined with NuPage LDS sample buffer 4x (#2083421) and 5% 2-Mercaptoethanol (Sigma Aldrich #M6250) and heated for 10 minutes at 70°C ...
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bioRxiv - Synthetic Biology 2019Quote: Replica-plated cell populations were treated with varying concentrations of 5-aza-2’-deoxycytidine (Sigma-Aldrich, A3656) or Trichostatin A (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... GluR inhibition was performed using 40 µM D(-)-2-amino-5-phosphonopentanoic acid (APV, #A8054, Sigma Aldrich) and 2 µM 6,7-dinitroquinoxaline-2,3-dione (DNQX ...
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bioRxiv - Cell Biology 2021Quote: ... before fixation for 5 hrs in humid conditions at 37 °C in 1.4% / 2% formaldehyde (F8775 Sigma) / acrylamide (A4058 Sigma) ...
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bioRxiv - Neuroscience 2020Quote: All birds received intramuscular injections of 5-Bromo-2’-deoxyuridine (BrdU; 74 μg/g, pH 7.4, Sigma) 3x/day for three days to label mitotically active cells ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 4-amino-5 methylamino-2’ s,7’-difluorofluorescein (DAF-FM) diacetate (Sigma, D1946). DAF fluorescence intensities were measured every 5 minutes for a total of 60 minutes using a SpectraMax iD3 (Molecular Devices) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked for 2☐h in 5% bovine serum albumin (BSA, Sigma-Aldrich, A7906) in 0.01☐M PBS and 0.3% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The parasites (ring stage; 2-4 hpi) were tightly synchronized with 5% (v/v) D-sorbitol (Sigma) and then monitored by Giemsa staining of methanol-fixed blood smears ...