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Citations for Millipore Sigma :
3201 - 3250 of 10000+ citations for 6 Chloro 5 fluoro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... and kept at 4°C for 1h in KB solution containing 50 mg/ml polyvinylpyrrolidone (PVP, Sigma, Chemical Co.). Finally ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... The lysates were then precleared for 1h at 4°C using 10µl protein A agarose 50% slurry/plate (Millipore), and collected by centrifugation at 300g for 3min at 4°C (100µl of sample/plate was kept as input chromatin) ...
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bioRxiv - Developmental Biology 2021Quote: ... plates were coated with fibronectin (Human Plasma Fibronectin Purified Protein, Sigma-Aldrich, 5µg/ml in PBS, 1h at RT).
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bioRxiv - Immunology 2022Quote: ... was applied for 1h prior to staining with the indicated primary antibody diluted in PBS solution with 0.5% Goat Serum (Sigma), 0.5% BSA (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 1H NMR was acquired with 64 scans on a Jeol 400 MHz spectrometer using deuterated water (D2O; Sigma-Aldrich) as an internal standard ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were permeabilized for 1h at room temperature in 0.1 M PBS containing 0.5% Triton X-100 (Sigma Aldrich), followed by 1.5 h incubation in blocking solution of 0.1 M PBS containing 0.3% Triton X-100 and 5% Normal Donkey Serum (NDS ...
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bioRxiv - Immunology 2023Quote: ... the reaction mixture was incubated at 37°C for 1h and its aconitase activity was determined per manufacturer’s instructions (Aconitase Assay Kit from Sigma).
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bioRxiv - Molecular Biology 2023Quote: ... sample could be incubated also for 1h with oligo-dT-dig probe (1:1000, polyT-dig Sigma HA09131354-004). Antibodies were diluted 1:500 in blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... the blot was again washed with block buffer and incubated for 1h with either Monoclonal ANTI-FLAG M2 (Sigma) (1:7500 dilution ...
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bioRxiv - Biophysics 2023Quote: ... samples were incubated with the following primary antibodies for 1h: mouse anti-α-Tubulin (1:1000, T5168, Sigma-Aldrich) and rabbit anti-Lamin A (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: Coverslips with either adult mouse or neonatal rat cardiomyocytes were incubated 1h with anti-α-actinin antibody (A7811, Sigma) at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed samples were washed rapidly twice with PBS and 3 times for 15 min with PBS-0.3% Triton X-100 (PBT) before being pre-incubated for 1h in PBT-1% bovine serum Albumin (BSA, Sigma). Samples were incubated overnight at 4°C with primary antibody diluted in PBT-1% BSA ...
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bioRxiv - Developmental Biology 2024Quote: ... for 1 min 30 seconds at high intensity in a 200 mTorr vacuum and then incubated at room temperature for 1h with 200µL/well of 200 µg/mL Poly-D-Lysine (A-003-E, Merck Millipore) diluted in 0.1M HEPES pH 8.4 (H3784-25G ...
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bioRxiv - Microbiology 2024Quote: ... They were then incubated for 1h with an anti-rabbit IgG conjugated to horseradish peroxidase (HRP) (A9169; Sigma-Aldrich) as the secondary antibody at a dilution of 1:10,000 ...
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bioRxiv - Developmental Biology 2021Quote: PAs from homozygous a2KO (n = 5) and WT (n = 6) embryos were manually dissected in ice-cold DEPC-treated phosphate-buffered saline (DPBS, Sigma catalog number D8537). Individual PAs were pooled into four samples (WT PA1 ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 and 6 were pooled and concentrated to 1 ml using 10 kDa centrifugal filter (Amicon Ultra-2ml, Merck Millipore, Billerica, Massachusetts, USA). The resulting 1 ml sample was loaded on top of a DG ...
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bioRxiv - Plant Biology 2020Quote: ... and their petioles dipped in 1 mM 5(6)-Carboxyfluorescein diacetate (CFDA, Ex/Em 492/517 nm, Millipore-Sigma, St. Louis, MO, USA) for 5 min ...
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bioRxiv - Plant Biology 2020Quote: ... and their petioles dipped in 1 mM 5(6)-Carboxyfluorescein diacetate (CFDA, Ex/Em 492/517 nm, Millipore-Sigma, St. Louis, MO, USA) for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were transferred to 6 well culture dishes and rehydrated with MilliQ water and labeled in H2O for 5 min with Dapi (Sigma-Aldrich, Cat # 9542) 1:10,000 dilution of 1 mg/ml aqueous stock and Syber Gold 1:20,000 dilution of DMSO stock (ThermoFisher Cat # S11494) ...
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bioRxiv - Biophysics 2019Quote: ... Membrane was washed another 6 times in TBS-T then developed in the dark for 5 min with chemiluminescent reagent (Immobilon Forte, Millipore, Burlington, MA USA).
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bioRxiv - Microbiology 2022Quote: ... FISH probes were labelled with 5(6)-carboxyfluorescein-N-hydroxysuccinimide ester (FLUOS) or with the sulfoindocyanine dyes (Cy3) at the 5’ end of the probes (Sigma Aldrich company, Germany). FISH sample preparation was conducted as described earlier (Fernando et al. ...
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bioRxiv - Bioengineering 2024Quote: ... each well was filled with 400 µL of RPMI-B27 supplemented with 10µM Y-27632 and 5 mg/mL 6-aminocaproic acid (Sigma-Aldrich, cat. no. A7824). On day 1 ...
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bioRxiv - Physiology 2023Quote: ... Cells were incubated with P-gp substrate calcein-acetoxymethyl ester (Ca-AM, 177831, 10−6 M, Sigma; 37°C, 5% CO2, 1 hour). Once substrate was added ...
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bioRxiv - Cancer Biology 2021Quote: ... 3-methylamine (3-MA) and bafilomycin (Sigma Aldrich) were used ...
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bioRxiv - Cell Biology 2019Quote: The 3-Bromopyruvatic Acid (3-BrPA) powder (Sigma) was dissolved in PBS ...
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bioRxiv - Microbiology 2022Quote: ... and GIGYF2 shRNA#3 (shGIGYF2#3) (Sigma, TRCN0000135088).
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bioRxiv - Neuroscience 2020Quote: ... 3’-cyclic nucleotide 3’- phosphodiesterase (CNP, Millipore MAB326) and proteolipid protein (PLP ...
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bioRxiv - Cell Biology 2022Quote: ... 14-3-3 (Millipore AB9748-I, 1:2000); TOPO II (Abcam 109524 ...
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bioRxiv - Microbiology 2021Quote: ... + 3 μl benzonase (Novagen, Merck Millipore 70746-3), + 1 Roche complete protease inhibitor tablet) ...
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bioRxiv - Microbiology 2021Quote: ... + 3 μl benzonase (Novagen, Merck Millipore 70746-3), + 1 Roche complete protease inhibitor tablet) ...
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bioRxiv - Cell Biology 2020Quote: ... a 3-30% or 3-15.5% OptiPrepTM (Sigma) continuous gradient was prepared in a buffer containing 78 mM KCl ...
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bioRxiv - Neuroscience 2021Quote: ... DAB (3-3’-diaminobenzidine, Sigma-Aldrich, D8001-10G) was dissolved in 0.1 N HCl at a concentration of 5.4 mg/ml and subsequently diluted ten fold into blocking solution ...
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bioRxiv - Immunology 2020Quote: ... 3-NP (3-Nitropropionic acid, 62.5 μM; Sigma) and water-soluble cholesterol (50 mM ...
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Community-based Reconstruction and Simulation of a Full-scale Model of Region CA1 of Rat HippocampusbioRxiv - Neuroscience 2024Quote: ... and then in DAB (3, 3’ diaminobenzidine, Sigma) to reveal the morphology of the recorded neurons ...
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bioRxiv - Cell Biology 2024Quote: ... 10 mM 3-methyl adenine (3-MA, Sigma), 5 µM neutral sphingomyelinase inhibitor GW4869 (SelleckChem) ...
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bioRxiv - Bioengineering 2022Quote: ... 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma) and N-hydroxysulfosuccinimide (Sulfo-NHS ...
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bioRxiv - Molecular Biology 2021Quote: ... Apoptotic cell death was detected by annexin-V/propidium iodide staining and subsequent flow cytometry analysis using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Annexin-V-FITC emission was detected with the green filter at 525/530 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... washed and resuspended in PBS and measured by flow cytometry (FACS) using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Fluorescence was excited at 488nm and BODIPY emission was recorded at 530 and 585 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 x105 HEK293 cells were seeded in each well of a 6-well plate and were transfected with 2 μg of respective plasmid DNA using PEI reagent (Sigma-Aldrich, 408727). After 24 hours ...
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bioRxiv - Genomics 2019Quote: ... and 12.5 ng/µL oligo(dT) at 42°C for 2 h in the presence of 6 ng/µL actinomycin D (Sigma, #A1410-2MG) to prevent antisense artifacts (Perocchi et al ...
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bioRxiv - Biophysics 2022Quote: The dyes used in this study and the working dilutions were: 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine (LAURDAN, 4.5 μM, Sigma-Aldrich, Merck, #40227), Hoechst 33342 (80 uM ...
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bioRxiv - Zoology 2019Quote: ... or cortisol treatments (8 g peanut butter, 2 g wheat germ with 6, 8, or 12 mg of cortisol [H4001, Sigma Aldrich, USA]). Dosages of 0 ...
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bioRxiv - Immunology 2019Quote: ... CHO cells pre-incubated with trivalent Strep-Tactin-SpyCatcher or buffer alone were incubated with 2 µM ATTO 647 biotin (ATTO Technology # AD 647-71) pre-mixed with 6 µM biotin (Sigma-Aldrich # B4501) for 30 minutes at 25 °C ...
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bioRxiv - Neuroscience 2020Quote: ... After rinsing three times for 10 minutes in TBS at RT sections were incubated with 4?,6-Diamidin-2-phenylindol (Dapi, Sigma, Cat# D9542) for 10 minutes at RT and washed once for 10 minutes in TBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and urethane (ethyl carbamate, EC; CAS# 51-79-6) from Sigma (St. Louis, MO). Primer sets were Kras ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell nuclei and actin filaments were stained simultaneously with 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI; 1:2000, Sigma-Aldrich, Cat# D9542) and Phalloidin-Atto 647N (1:2000 ...
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bioRxiv - Genomics 2022Quote: ... culture medium was replaced with S1-media (Final 11,6 g/L MCDB131, Sigma Aldrich, M8537-1L; 2 mM D-+-Glucose, Sigma Aldrich, G7528-250G; 2,46 g/L NaHCO3, Sigma Aldrich, S5761-500G ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated for two hours with Alexa Fluor® 647 labeled secondary antibody (1:500), and nuclei were labeled with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Sigma-Aldrich, #D8417). The fluorescent images were obtained with a Zeiss Axio Observer inverted microscope (40 × NA 1.3 oil objective ...
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bioRxiv - Cell Biology 2024Quote: Uninduced and induced samples were treated with either 0.2 μM or 2 μM Latrunculin A in DMSO (Sigma, CAS 76343-93-6) up to 4 h at 37 °C ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... the slide was washed three times with the wash buffer and stained sequentially with the Immunol Fluorence Staining Kit with Alexa Fluor 647-Labeled Goat Anti-Rabbit IgG (P0180, Beyotime, China) and 4’,6-diamidino-2-phenylindole (DAPI, MBD0015, Sigma-Aldrich, USA) as per the manufacturers’ instructions ...