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Citations for Millipore Sigma :
3651 - 3700 of 10000+ citations for 6 Chloro 5 fluoro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were inoculated in YEP containing 3% lactic acid and treated with a final concentration of 2% galactose (Sigma-Aldrich). For the inducible degradation of the proteins ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mm3 tissue pieces from 2-3 brains were pooled and enzymatically digested in a dissociation medium containing 0.16 mg/mL cysteine (Sigma C9768), 7 U/mL Papain (Sigma P3125) ...
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bioRxiv - Developmental Biology 2023Quote: ... lentiviruses were generated by transfecting 3 x 106 HEK-293T cells in antibiotic-free medium with 6 µg of total DNA in a 1:2:3 ratio of pMD2.G:psPAX2:transfer plasmid using polyethylenimine (PEI) (Sigma-Aldrich). 48 h after transfection ...
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bioRxiv - Systems Biology 2024Quote: ... 2M thiourea, 100mM Tris-HCl, pH 8, 150mM NaCl, 1mM EDTA, phosphatase inhibitor cocktail 2 and 3, 10mM NaF; Sigma), reduced with 10mM DTT (dithiothreitol ...
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bioRxiv - Neuroscience 2024Quote: ... and 2-(14-carboxytetradecyl)-2-ethyl-4,4-di methyl-3-oxazolidinyloxy (16-doxyl-stearicacid, 16-DSA, cat. no. 253596) from Sigma-Aldrich. 5-DSA (spin probe containing the nitroxide group attached on C5 that is located on the opposite terminal relative to the charged carboxyl fatty acid terminus ...
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bioRxiv - Immunology 2023Quote: ... The activity of trypsin was inhibited by dropping the pH to 2-3 by the addition of 10% trifluoroacetic acid (TFA, Sigma). The samples were loaded on Evosep tips to separate the digested peptides using nanoflow reversed-phase chromatography with an Evosep One liquid chromatography (LC ...
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bioRxiv - Plant Biology 2024Quote: Arabidopsis Ws-2 CCR2::LUC WT seeds were surface sterilized and plated on 1x MS medium containing 3% w/v sucrose (Sigma), 1.5% w/v phytoagar (Duchefa) ...
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bioRxiv - Bioengineering 2024Quote: ... and polyIC incubation was evaluated using the MTT (3-(4, 5dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). The cells were seeded into 96 well-format cell culture plates at a seeding density of 8ξ103 cells per well ...
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bioRxiv - Neuroscience 2021Quote: ... The left MFB was injected with either 1μL of 6-Hydroxydopamine hydrochloride (6-OHDA, (Sigma-Aldrich) 3.2µg/µL free-base in a 0.02% ascorbic acid solution (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: Serum IL-6 levels were quantified using a commercial mouse IL-6 ELISA kit (RAB0308, Sigma) according to the manufacturer’s instructions and analyzed using spectra-iMAX (Molecular Devices).
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bioRxiv - Cell Biology 2021Quote: ... 6 mM ATP or 6 mM ADP (conjugated with sodium salt; Sigma-Aldrich, St. Louis MO) were included along with the contents described above ...
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bioRxiv - Neuroscience 2021Quote: ... P3 and P6 pups were injected intraperitoneally with 50 mg/kg of EdU (5-ethyl-2-deoxyuridine; Sigma). Brains were harvested 2h after injection and processed for frozen tissue sections ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cultured overnight at 37 °C in 5% CO2 in neurobasal medium supplemented with 2% B27 (Sigma-Aldrich) and containing 50 ng/mL GDNF (PeproTech ...
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bioRxiv - Genetics 2019Quote: ... Seedlings were harvested and ground in liquid nitrogen and total protein was extracted in IP buffer (50 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM EDTA, 10% glycerol, supplemented with 5 mM DTT, 0.5 mM PMSF, Sigma protease inhibitor cocktail P9599 ...
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bioRxiv - Biochemistry 2021Quote: ... with 10% ADC supplement (5% BSA, 0.85% NaCl, 2% dextrose) and 0.05% tyloxapol or 7H10-ADC agar medium (Sigma) at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... rats were injected intraperitoneally with 100 mg/kg 5-Bromo-2′-deoxyuridine (BrdU, Sigma-Aldrich, St. Louis, MI) dissolved in warm 0.9% saline immediately after release from Decapicone bags ...
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bioRxiv - Neuroscience 2020Quote: ... (+)-Bicuculline (No. 14340) and DL-2-amino-5-phosphonopentanoinc acid (APV; No. A5282) were obtained from Sigma-Aldrich; 6,7-dinitroquinoxaline-2,3(1H,4H)-dione disodium salt (DNQX ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue was then incubated in CUBIC-1A solution (10% Triton, 5% NNNN-tetrakis (2-HP) ethylenediamine (Sigma, 122262), 10% Urea ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were stimulated with 50 U/ml IL-2 and 5◻μg/ml phytohemagglutinin (PHA; Sigma-Aldrich) for 72 h prior to infection or transduction ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with 0.1 mL serially diluted supernatant containing SARS-CoV-2 for 30 minutes at 37°C and 5% CO2 before being left in 1.5mL overlay (DMEM, 2% FBS, 1.2% cellulose microcrystalline (Sigma)) at 37°C and 5% CO2 for 72 hours ...
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bioRxiv - Neuroscience 2020Quote: ... After a 2 h blocking step in blocking buffer (PBX with 5% normal goat serum, Sigma-Aldrich, G9023) at RT ...
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bioRxiv - Immunology 2022Quote: ... in PBS 30 minutes at RT and blocked with 2% BSA and 5% Donkey Serum (Sigma-Aldrich, D9663) in PBS during 1 h at RT ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM MgCl2) for 2 min and then the DNA digested with 20U DNaseI (cat #: D4527, Sigma-Aldrich) in 1 mL of digestion buffer for 40 sec at RT ...
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bioRxiv - Genetics 2022Quote: ... Experiments for Figure 5 were as Figure 2 with the following changes: 10 μg/mL tunicamycin (InSolution, Sigma) was used ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed using 5% SDS supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich). The following antibodies were purchased from Cell Signaling Technology (Danvers) ...
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bioRxiv - Immunology 2020Quote: ... Primary antibody in 250 μl of cold antibody buffer (20 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 2 mM EDTA, 0.1% digitonin, and protease inhibitor cocktails from Sigma) was added to the tubes and rotated at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... and Click-iT® EdU (5-ethynyl-2’deoxyuridine) Assay (BCK-EDU488, baseclick GmbH, Merck/Sigma-Aldrich, UK), respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells in Accutase were added 1:2 to mTeSR™1 plus 5 μM Blebbistatin (Bleb, B0560, Sigma), pelleted at 150g for 5 minutes ...
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bioRxiv - Genetics 2019Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma-Aldrich, St. Louis, MO, product C4706) and alkylated with 55 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Genomics 2021Quote: ... 10 mM MgCl2,10 mM Tris pH 7.4, 1 mM CaCl2) with increasing amounts (0, 0.5, 1, 2, 5, 7.5, 10 U) of MNase (Sigma) at 30°C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... zebrafish embryos were treated with 180 µM of 1-phenyl 2-thiourea (PTU) (Sigma-Aldrich, 103-65-5), to prevent pigmentation [25] ...
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bioRxiv - Cancer Biology 2024Quote: ... β-estradiol was removed at day 5 from Type 2 medium and DMSO (Sigma, #D2650; final concentration = 0.1%) or 4-hydroxytamoxifen (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were carried out using the indicated antibodies: α-Tubulin (Sigma, B-5-1-2; T5168, 1:100,000), BLM (Bethyl ...
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bioRxiv - Neuroscience 2023Quote: ... the larvae were anaesthetized with 0.02% Tricaine for 5-10 minutes and immobilized in 1.5-2% low-melting point agarose (Sigma) in fish water ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Cell Biology 2022Quote: ... reactions were incubated for 2 h on ice before quenching with 5 mM L-Cysteine (Millipore-Sigma, USA). The reactions were then adjusted to 1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... reactions were incubated for 2 h on ice before quenching with 5 mM L-Cysteine (Millipore-Sigma, USA). The reactions were then adjusted to 1% (w/v ...
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bioRxiv - Immunology 2023Quote: ... 2% FCS and 50 μg/ml Lipopolysaccharide neutralizing agent Polymyxin B sulfate (cat # 1405-20-5; Sigma-Aldrich) for 30 min at 37°C while shaking by 750 rpm on a heating block ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells in Accutase were added 1:2 to mTeSR™1 with 5 µM Blebbistatin (Bleb, B0560, Sigma), and pelleted at 150 g for 5 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... Immunofluorescence studies were performed on 5 µm sections fixed in 2% paraformaldehyde (PFA; Millipore Sigma, St. Louisa, MO) for 5 minutes at room temperature (RT).
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bioRxiv - Microbiology 2023Quote: ... VirB or its mutant variants were incubated with 5 mM Tris(2-carboxy-ethyl) phosphine (TCEP; Sigma, USA) for 1 h at room temperature to fully reduce all cysteine residues ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody65 (kind gift of G ...
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bioRxiv - Neuroscience 2023Quote: ... diluted in PBS + 5% NDS and in the second staining (2) 2B4 (1:200, Sigma Eldrich, Cat. # MAB5492), GFAP (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Stock solutions of the NMDA receptor antagonist DL-2-amino-5-phosphonovaleric acid (DL-APV; #5282; Millipore Sigma), GABAA receptor antagonist bicuculline methiodide (#14343 ...
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bioRxiv - Cell Biology 2024Quote: ... All media used for compound dilutions contained 20 mM glucose and 2% DMSO (Sigma Cat. #D2650-5×5ml).
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bioRxiv - Cancer Biology 2024Quote: ... The treatment group received 2 mg/ml Dox (BelaDox) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich), to induce an in vivo knockdown ...
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bioRxiv - Immunology 2024Quote: One million of purified human CD4+ Tsen was activated with an anti-CD3 antibody coated-plate (0.5μg/ml) plus rhIL-2 (5 ng/ml; 11 011 456 001; Sigma). Forty-eight hours post-activation ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.5 and reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich Cat No: C4706) for 30 minutes at room temperature ...