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Citations for Millipore Sigma :
3301 - 3350 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... followed by staining with 4’,6-diamidino-2-phenylindole (DAPI, 1:10,000 dilution with milliQ water, Sigma-Aldrich) for 10 minutes ...
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bioRxiv - Physiology 2020Quote: ... Nuclei were counter-stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 1/5000) (Sigma-Aldrich, Dorset, UK). Imaging was performed on a Leica TCS SP5 confocal microscope (Leica Microsystems ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of T4 DNA ligase buffer (10x stock) and 6 μl of PEG-4000 (Sigma Aldrich, 50%) per amplicon pool reaction ...
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bioRxiv - Cancer Biology 2021Quote: ... We also used primary mouse monoclonal antibodies against anti-α-acetylated tubulin (clone 6-11B-1, Sigma Aldrich) for detecting mitotic spindles as described in Maiorova and Odintsova ...
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bioRxiv - Immunology 2020Quote: Cell suspensions were stained for viability with either 1:3000 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) or 1:1000 Zombie Aqua (Biolegend ...
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bioRxiv - Neuroscience 2022Quote: ... with 1:30,000 of the fluorescent marker of nucleic acids 4’,6-diamino-2-fenilindol (DAPI; Sigma-Aldrich). We took photographs of the cells using an epifluorescence microscope (DMIL LED ...
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bioRxiv - Molecular Biology 2022Quote: ... the nuclear pellet was resuspended in buffer C (40 mM HEPES pH 7.6, 4 mM MgCl2, 0.6% Triton X-100, 0.5% IGEPAL CA-630, 20% Glycerol, 1 mM DTT, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse anti-acetylated tubulin (clone 6-11B-1) was Cat# T6793 (RRID:AB_477585) from Sigma-Aldrich (St. Louis, MO). Rabbit anti-GPSM2 Cat# HPA007327 (RRID ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) 1:1,000 (Sigma-Aldrich Sweden AB, Stockholm, Sweden). Invasion of the cells was measured by counting the nuclei of the cells that migrated through the filter pores towards the chemoattractant (6% FBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were harvested 6 hours post transfection through the addition of 1 mL TRI reagent (Sigma-Aldrich, Merck) directly to the monolayer ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were incubated with DAPI (4’,6-Diamidino-2-phenylindole dihydrochlorid) solution(D9542, Sigma-Aldrich, diluted 1:10000) for 7 minutes at RT and subsequently mounted in ProLong Gold Antifade mounting medium (cat ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used were acetylated α−tubulin (1:500, clone 6-11-B1, Sigma-Aldrich, St. Louis, MO), rabbit polyclonal DNALI1 (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... Enriched proteins were denatured in U/T buffer (6 M urea, 2 M thiourea, 1 mM DTT (Sigma), 10 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... 0.04 mg/ml catalase (EMD biosciences) and 1 mM 6-hydroxy-2,5,7,8-tetramethyl-chromane-2-carboxylic acid (Trolox) (Sigma Aldrich). After DNA was tethered on the surface ...
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bioRxiv - Physiology 2024Quote: ... and then moved into 6-well-plates containing digestion medium (DMEM + 10% FBS + 1% amphotericin B (A2942, Sigma)) and 10% liberase (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... and after cool down alkylated with 6 μL of freshly prepared 1 M iodoacetamide (IAA, Sigma, #I1149-5G). The alkylation was performed for 15 min at room temperature in darkness ...
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bioRxiv - Cancer Biology 2020Quote: ... The antibody reaction was visualized with 3-3’ diaminobenzidine (Sigma, D8001) followed by counterstaining with hematoxylin ...
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bioRxiv - Neuroscience 2022Quote: ... prior to being rinsed and reacted using 3-3’diaminobenzidine (Sigma) as chromagen ...
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bioRxiv - Biochemistry 2021Quote: ... and 3’,3’c-di-AMP were purchased from Sigma-Aldrich, 2’,3’-cGAMP ...
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bioRxiv - Immunology 2019Quote: ... 3 ml of 3% bacteria-free thioglycollate solution (Sigma, B2551-500G) was injected into the abdominal cavity of C57/BL6 mice ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 1.5 mM 3-amino-1,2,4-triazol (3-AT, Sigma). Selection plates were left for 8 days at 30°C ...
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bioRxiv - Systems Biology 2024Quote: ... or 3-bromopyruvate (3-BP; Sigma-Aldrich, St. Louis, MO; 16490) were added to their respective wells ...
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bioRxiv - Genetics 2022Quote: ... 5’-fluoroorotic acid (5’FOA; Sigma) was used at a concentration of 1 g/L in PMG containing 45 mg/L uracil ...
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bioRxiv - Biophysics 2020Quote: ... were added to 1 mL of 10 mg∙mL−1 of N-(3-dimethylaminopropyl)-N’-ethylcabodiimidie hydrochloride (EDC, Sigma-Aldrich) dissolved in 100 mM sodium phosphate ...
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bioRxiv - Cell Biology 2019Quote: ... After washing 3–4 X with 1× PBS-T the strips were incubated with 1:5000 mouse anti-GST antibodies (Sigma). The secondary antibodies (anti-rabbit-HRP or anti-mouse-HRP ...
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bioRxiv - Biochemistry 2020Quote: ... which was followed by the addition of NH4HCO3 (1 M, 3 μl, freshly made) and Calf Intestinal Alkaline Phosphatase (1 U, Sigma) and additional incubation at 37 °C for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated 1-3 overnights at 4°C in the following primary antibodies: goat anti-choline acetyltransferase (1:200; Millipore), rabbit anti-substance P (1:500 ...
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bioRxiv - Physiology 2021Quote: ... cells were differentiated into adipocytes using MDI medium (100 mg/ml 3-isobutyl-1-methylxanthine, 100 ng/ml dexamethasone and 1 mg/ml insulin, both from Sigma) for 4 days ...
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bioRxiv - Neuroscience 2020Quote: ... 3% Triton-X in PBS) for 1 h before incubation with primary antibodies (1:1000 anti-tyrosine hydroxylase, #AB152, Millipore UK ...
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bioRxiv - Cancer Biology 2020Quote: ... Colonies were fixed with Carnoy’s solution (1 acetic acid: 3 methanol) and stained with 1% (w/v) crystal violet (Sigma-Aldrich). The percentage of colonies covering the plates were calculated with ImageJ software.
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 10μM 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (18:1 NBD-PS) (1:300, Sigma-Aldrich) for 15 minutes at 25°C ...
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bioRxiv - Systems Biology 2021Quote: INS-1 cells (INS-1 832/3 rat insulinoma cell line) were cultured in RPMI-1640 (Sigma Cat. No. R0883) supplemented with 2 mM L-glutamine ...
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bioRxiv - Immunology 2023Quote: ... Th17 cells were split (usually 1:3) and transduced by adding 1:80-diluted retroviral supernatant and 4 µg/ml polybrene (Sigma) in addition to 10 ng/ml IL-23 and 10 µg/ml anti-IFNγ antibody (all diluted in pre-warmed T cell medium and calculated for culture volume) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates and immunoprecipitates were analyzed on Western blots using mouse monoclonal anti-MYC antibody (1:800; Covance) (for exogenous 14-3-3ε) or anti-FLAG antibody (1:800; Sigma) (for exogenous Cdc25B ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were blocked with 3% BSA/PBS for 1 hour and incubated with rabbit anti-Olig2 (1:500, Millipore), mouse anti-CC1 (1:100 ...
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bioRxiv - Plant Biology 2023Quote: 15 dpi nodules were hand sectioned and kept for 10 minutes in staining solution (1:3 ratio of 1:500 dilution of propidium Iodide (Sigma) and 1 ...
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bioRxiv - Immunology 2023Quote: ... followed by streptavidin-Cyanine-3 (1:500; Amersham Bioscience) or by goat anti-human IgG coupled to FITC (1:100, Sigma), as appropriate ...
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bioRxiv - Neuroscience 2023Quote: The tissue section on the Stereo-seq chip (1 cm x 1 cm) was then baking at 37°C for 3 min and subsequently fixed in methanol (Sigma, 34860 ...
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bioRxiv - Microbiology 2024Quote: ... RNA extraction was performed according to the manufacturer’s protocol except that 1 M 1-Bromo-3-ChloroPropane (BCP) (Sigma-Aldrich) was used instead of chloroform ...
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bioRxiv - Neuroscience 2024Quote: ... coverslips were incubated in 300 μL of primary antibody (anti-TDP-43 1:750, PTGLabs; anti-SUMO2/3 [8A2] 1:500 Sigma) diluted in blocking buffer (1.5% Triton X-100 ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 1 × 107 CFU were added to a flow cytometry tube containing 1 mL buffer with 10 μM DiOC2(3) (Sigma) and incubated in the dark for 30 min at 30 0C ...
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bioRxiv - Cancer Biology 2021Quote: Cell viability was determined using the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assay (MTT assay, Cat. No. M5655, Sigma-Aldrich, St. Louis, MO). Cells were plated in 96-well plates ...
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bioRxiv - Cell Biology 2020Quote: ... transfected cell lines were grown on 22×22 mm coverslips and then fixed as follows: 3-5 minutes in cytoskeletal buffer (Byron et al., 2013) plus 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO) and 10 mM ribonucleoside vanadyl complex (New England Biolabs ...
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bioRxiv - Cancer Biology 2019Quote: WT and DCLK1+ cells were plated at 3 x 10^5 cells/well into 8-well Millicell EZ chamber slides (Millipore, cat. no. PEZGS0816) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indoxyl phosphate (BCIP; Sigma Aldrich, Taufkirchen, Germany) for 5-30 min.
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bioRxiv - Microbiology 2021Quote: ... Transfected cells were supplemented three days after transfection with 3 g/L glucose (Bioconcept) and 5 g/L soy hydrolysate (Sigma-Aldrich Chemie GmbH). Cell culture supernatant (423 mL ...
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bioRxiv - Cell Biology 2019Quote: ... animals were boosted 5 times in a 3 week-interval with 0.2 mg human TNFa in incomplete adjuvant (Sigma Chemical, St. Louis, MO). Final boost was given intravenously with 0.4 mg the protein in PBS four days before splenectomy ...
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bioRxiv - Biochemistry 2021Quote: ... Chemotherapeutic drug-treated cells were incubated with 10 μL 3-(4,5)-dimethylthiazol (-z-y1)-3,5-diphenyltetrazolium bromide (MTT) solution (5 mg/mL, Sigma-Aldrich, St Louis, MO, USA) for 4 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 8-(4-chlorophenylthio) adenosine-3’,5’-cyclic monophosphate (CPT-cAMP; PKA activator) or PMA (PKC activator)) were purchased from Sigma-Aldrich (ON, Canada). Powdered CBD ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections were then rinsed with PBS (3×5 min at RT) and then cell nuclei were stained with a DAPI solution (Sigma-Aldrich, 1μg/mL) incubated for 5 min at RT ...