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Citations for Millipore Sigma :
3551 - 3600 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... plus 5% normal goat serum for 1 hr at RT before 3 d incubation in primary antibody diluted in block (1:1,000, chicken anti-GFP, Abcam, ab13970; 1:200 rabbit anti-cleaved caspase 3, EMD Millipore, AB3623). Sections were washed several times in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue samples from brain and peripheral organs were homogenized in 3:1 (v/w) of PBS containing 1% Triton X-100 supplemented with protease inhibitors (cOmplete™, Sigma) using the TissueRuptor (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... Cleaned tissue samples were incubated at 37 °C for 1 h in 10 mL DPBS with 1 mM DTT and 3 mM EDTA (Sigma-Aldrich). After incubation ...
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bioRxiv - Systems Biology 2020Quote: ... 120μL of 1:1 DTNB:GR solution (DTNB: 2mg 5,5’-dithiobis(2-nitrobenzoic acid) (Sigma-Aldrich; Cat#D8130; CAS: 69-78-3) in 3mL KPE ...
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bioRxiv - Cancer Biology 2020Quote: ... pelleted, and re-suspended in lysis buffer (1 mM CaCl2, 3 mM MgCl, 1mM EDTA, 1% Triton X-100 (Sigma-Aldrich), 10 mM TrisHCl ...
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bioRxiv - Microbiology 2021Quote: ... Allicin was synthesized by oxidation of 3-[(Prop-2-en-1-yl)disulfanyl]prop-1-ene (diallyl disulfide, Sigma-Aldrich, Germany) with peracetic-acid (glacial acetic acid/H2O2 ...
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bioRxiv - Bioengineering 2022Quote: ... A stock solution of 3 mg/mL rat tail collagen type I was neutralized with 1% 1 M NaOH (Sigma, S5761) and 10% 10x phosphate buffered saline ...
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bioRxiv - Microbiology 2024Quote: ... parasite pellets were placed on ice for 5 min before addition of 50 μl chloroform followed by 200 μl methanol:ultrapure water (3:1, including scyllo inositol as an internal standard, 1 nmol, Sigma-Aldrich, I8132). Extraction was facilitated through vigorous vortexing ...
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bioRxiv - Microbiology 2024Quote: For the leukopenic model mice were immunosuppressed with 150 mg/kg of cyclophosphamide (Baxter) on days −3 and −1 plus 1 subcutaneous injection of 250 mg/kg hydrocortisone 21-Acetate (Sigma-Aldrich) on day −1 ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 1% BSA, 1 mM DTT, 0.1 U/µl Sigma Protector RNase Inhibitor) was then added to the cell lysis and the nuclei were pelleted by centrifuging at 500xg for 5 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with fresh protease inhibitors (3 µg/mL Leupeptin, 1 µg/mL macroglobulin, 1 µM benzamidine, 0.5 µM PMSF) and phosphatase Inhibitors (EMD Millipore, 524625). Cell lysates were incubated 10 minutes on ice and centrifuged at 13000 rpm for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... The cells were maintained with DMEM+10%FCS for 3 hours and fixed with acetone/methanol (1:1 ratio) and stained with Giemsa stain (Sigma-Aldrich). Stained cell images were taken by EVOS XL imaging system (Life Technologies).
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bioRxiv - Cancer Biology 2024Quote: ... followed by dilution of the supernatant with fresh medium in a ratio of 1:1 and addition of 3 µg/mL hexadimethrine bromide (polybrene, Sigma-Aldrich). 5 µg/mL Puromycin were added and maintained to select for positively transduced cells ...
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bioRxiv - Biochemistry 2024Quote: ... were homogenized using a Polytron in 1 ml of 20 mM sodium acetate buffer containing 0.15 M NaCl and 1% Zwittergent 3-14 detergent (Millipore-Sigma 693017), pH 4.75 ...
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bioRxiv - Synthetic Biology 2024Quote: ... filtered spent media collected from a confluent flask) at the ratio of 1:3 to fresh medium with 25% FBS and 1% penicillin-streptomycin (Penicillin-Streptomycin, Sigma Aldrich). Confluent wells were then assessed with fluorescence microscopes ...
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bioRxiv - Cell Biology 2020Quote: 5*106 THP-1 cells were treated with doxycycline (200 ng/ml, Sigma) for 5 h ...
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bioRxiv - Cell Biology 2019Quote: ... 5 μg/mL Hoechst 33342 in 1 % (wt/vol) BSA (Sigma-Aldrich A9647) and incubated for 30 min in the dark ...
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bioRxiv - Immunology 2021Quote: ... supplemented with sterile-filtered mixture of 5 ml HEPES buffer (1 M; Sigma), 10 ml MEM nonessential amino acid solution (100X ...
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bioRxiv - Cell Biology 2022Quote: ... (B-5-1-2 coupled to Alexa-488, Thermo Fisher or DM1A, Sigma), rat anti-tyrosinated tubulin (YL1/2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5% penicillinstreptomycin (1 unit penicillin/mL and 0.1 mg streptomycin/mL, Sigma, P4333). HEI-OC1 cells were grown at 33°C in the presence of 10% CO2 ...
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bioRxiv - Biophysics 2020Quote: ... and 10 μL of 5 mg∙mL−1 of protein A (Sigma-Aldrich), were added to 1 mL of 10 mg∙mL−1 of N-(3-dimethylaminopropyl)-N’-ethylcabodiimidie hydrochloride (EDC ...
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bioRxiv - Biochemistry 2019Quote: ... grids were dipped into 5 μM 1-pyrenecarboxylic acid (Sigma Aldrich, Munich, Germany) dissolved in DMSO (Sigma Aldrich ...
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bioRxiv - Bioengineering 2019Quote: ... with 1% v/v surfactant (Span 80/Tween 80, HLB = 5, Sigma Aldrich) and homogenized on a PRO Scientific D Series homogenizer (Oxford ...
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bioRxiv - Plant Biology 2020Quote: ... a 1:5 dilution of Lugol’s iodine solution (Sigma-Aldrich, St. Louis, USA) was applied to the grain sections ...
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bioRxiv - Cell Biology 2021Quote: ... mouse mAb anti-α-Tubulin (Sigma-Aldrich, clone B-5-1-2, #T5168); mouse mAb anti-Flag M2 (Sigma-Aldrich #F1804) ...
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bioRxiv - Biochemistry 2021Quote: ... Monoclonal anti-alpha-tubulin clone B-5-1-2 antibody (T5168, Sigma-Aldrich) was used as the loading control ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclear DNA was counterstained with 5 μg mL−1 Hoechst 33342 (Sigma-Aldrich) and the coverslips washed and mounted with Vectashield mounting medium (Vector laboratories ...
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bioRxiv - Microbiology 2022Quote: ... the filters were blocked for 1 hr with 5% skimmed milk (Sigma-Aldrich) in 2x Tris Buffered Saline/Tween 20 (20 mM Tris–HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... whilst 1 μM of the membrane potential-sensitive dye DiSC3(5) (Sigma-Aldrich) was added 5 min prior to imaging ...
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bioRxiv - Immunology 2020Quote: ... with 1 mg/kg defactinib or vehicle (5% DMSO, 2.5% Solutol HS (Sigma), 2.5% absolute ethanol ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were blocked for 1 h with 5% milk (Sigma-Aldrich, Dorset, UK) (in tris-buffered saline and 1% Tween 20 (TBST) ...
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bioRxiv - Biochemistry 2022Quote: ... and 5 mM MgCl2) with or without 1 mM AMP-PNP (Sigma Aldrich). Change in anisotropy relative to FAM-nucleic acid was plotted as a function of CasDinG concentration ...
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bioRxiv - Microbiology 2022Quote: Cells (1×106) were pre-treated with concanamycin A (5 nM; Sigma-Aldrich) for 1 h at 37°C and inoculated with B.1 virus at an MOI of 0.01 in the presence of concanamycin A for 1 h at 4°C ...
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bioRxiv - Immunology 2019Quote: ... was oxidised by incubating at 1 mg/ml with 5 µM CuSO4 (Sigma) in PBS for 18 h at 37 °C ...
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bioRxiv - Microbiology 2019Quote: ... 5% glycerol) supplemented with 1 mM PMSF and 0.25 mg/mL lysozyme (Sigma). Cell debris was removed by centrifugation at 20000 g for 40 min at 4 °C and the lysate incubated with Ni-NTA Agarose Beads (Qiagen) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Hoechst 33342: 5 μg/mL) in 1% (wt/vol) BSA (Sigma-Aldrich A9647) and incubation for 30 min in the dark ...
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bioRxiv - Immunology 2021Quote: ... 500 ml supplemented with filter-sterilized 5 ml HEPES buffer (1 M; Sigma), 10 ml MEM nonessential amino acid solution (100x ...
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bioRxiv - Immunology 2020Quote: ... for 1 h at RT in microscopy buffer (5% w/V BSA (Sigma), 0,06% w/V saponin (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... 5 μg of S2 was mixed with 1 mg of polymyxin B (Sigma) immediately before subcutaneous deposition ...
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bioRxiv - Immunology 2020Quote: ... then 1 ml supernatant containing proper lentivirus and 5 μg/ml polybrene (Sigma) was added ...
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bioRxiv - Neuroscience 2021Quote: ... incubated in PBST with DAPI (1:10,000 of 5 mg/mL, Sigma-Aldrich) for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... incubated in PBST with DAPI (1:10,000 of 5 mg/mL, Sigma-Aldrich) for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... overnight in secondary antibody + DAPI (1:10,000 of 5 mg/ml, Sigma-Aldrich) in 0.3% PBS/Triton X-100 and 10% normal donkey serum ...
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bioRxiv - Molecular Biology 2022Quote: ... containing 10 μM EdU and 1 μM 5-fluoro-2’-deoxyuridine (Sigma-Aldrich). Images were acquired with a DeltaVision Elite system and nuclear fluorescence signals quantified using custom software as described(10) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... mice were injected with 1 mg 5’-bromo-2’-deoxyuridine (BrdU; Sigma #B9285). Following removal ...
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bioRxiv - Biochemistry 2024Quote: ... IGBMC) in 5% milk or in primary PRC1 antibody (1:1000, Sigma-Aldrich #HPA034521 ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 hour and then stimulated with LPS (5 μg/mL, Sigma–Aldrich) for 4 h to induce NET formation ...
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bioRxiv - Molecular Biology 2024Quote: ... and monoclonal anti-α-tubulin antibody (clone B-5-1-2, Sigma-Aldrich). After incubation with primary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... After blocking nonspecific binding sites for 1 h using 5% dry milk (Sigma) in Tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2022Quote: ... Carbon fiber electrodes were calibrated with 1 μM 5-HT (Serotonin HCl, Sigma) at the end of the experiment to convert peak current amplitude of 5-HT transients to concentration ...