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Citations for Millipore Sigma :
3101 - 3150 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... in a 1:3 ratio (μg DNA/μg PEI) in DMEM (Sigma-Aldrich, D6429) without supplements ...
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bioRxiv - Molecular Biology 2022Quote: ... THP-1-derived macrophages were treated with DMSO or 3 mM Itaconic Acid (Sigma) for 10 hours prior to actinomycin D treatment ...
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bioRxiv - Physiology 2022Quote: ... HepG2 cells were pretreated with 3-isobutyl-1-methylxanthine (0.1 mM, IBMX, Sigma-Aldrich) for 30 min and then stimulated with α-MSH or the selective MC1-R agonist LD211 (0.1 nM ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM Benzamidine) containing phosphatase inhibitor cocktail 2 and 3 (Sigma, P5726 and P0044). Supernatants containing proteins were collected after centrifugation at the highest speed for 15 minutes at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... 1 mM DTT) and concentrated using a 3 kDa MWCO Centriprep concentrators (Merck Millipore). For best performance in the iSAT reaction ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 3:1 methanol:acetic acid (MAA, Sigma-Aldrich, #322415 and A6283) and stored at -20 °C in 70% ethanol until use.
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with a cocktail of 1:100 phosphatase inhibitors cocktail 2 and 3 (Sigma/Merck ...
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bioRxiv - Biophysics 2023Quote: ... After 1-hour incubation in blocking buffer (3% bovine serum albumin (BSA, Sigma-Alrich) in BRB80 buffer) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... then washed 3 times with PBS before staining with DAPI (1 μg/ml, Sigma) for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... Anaesthetized 3 dpf zebrafish larvae were mounted in 1% low-melting point agarose (Sigma), perpendicular to a glass slide in a Petri dish filled with electroporation buffer (180mM NaCl ...
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bioRxiv - Developmental Biology 2023Quote: ... Collection medium was supplemented with 0.2 mM IBMX (3-isobutyl-1-methylxanthine; Sigma-Aldrich) to prevent meiotic resumption prior to culture ...
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bioRxiv - Neuroscience 2023Quote: ... rinsed in deionised water and transferred to 1% (3-aminopropyl)trimethoxysilane (Sigma-Aldrich, 281778) in methanol supplemented with 5% acetic acid for 20 min incubation in the dark ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... with a 3:1 mixture of PLGA and PEG-PLGA (Sigma Aldrich, Darmstadt, Germany) as polymers ...
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bioRxiv - Cell Biology 2024Quote: ... at 1:20 for 30 min followed by a 3 µM DAPI (D9542, Sigma) solution for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... anti-Par-3 rabbit pAb (#07-330, Sigma-Aldrich; 1:100 dilution for immunofluorescence), anti-β-catenin mouse mAb (#MAB2081 ...
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bioRxiv - Neuroscience 2024Quote: ... and the WNT pathway inhibitor IWR-1 (3 µM, cat #681669, Millipore, Sigma-Aldrich). On DIV 2 ...
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bioRxiv - Neuroscience 2024Quote: ... and the WNT pathway inhibitor IWR-1 (3 µM, cat #681669, Millipore, Sigma-Aldrich). On DIV 2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The primary and secondary antibodies were 1:2000 rabbit α-GAPDH (Sigma-Aldrich)/3% BSA/TBSA and 1:20,000 HRP-conjugated goat α-rabbit IgG (Sigma-Aldrich)/1% BSA/TBS ...
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bioRxiv - Cancer Biology 2024Quote: ... and 3 weeks with or without (1 μg/ml) of doxycycline (EMD Millipore: #324385), organoids were harvested and washed twice with cold PBS ...
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bioRxiv - Plant Biology 2024Quote: ... pre-treated with the B-chelator Amberlite IRA-743 (3 g L-1; Sigma) to prevent any B contamination.
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bioRxiv - Bioengineering 2020Quote: ... cells (15·106 mFBs·cm−3 and 30·106 THP-1 cells·cm−3) were added to a mixture of bovine fibrinogen (10 mg·ml−1; Sigma, F8630) and bovine thrombin (10 IU·ml−1 ...
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bioRxiv - Biophysics 2020Quote: ... The protein was eluted from the column with Wash Buffer supplemented with 3 mg mL-1 1D4 peptide and concentrated to 4.3 mg mL-1 using a 100-kDa concentrator (Millipore) before further purifying by size exclusion chromatography using a Superose 6 increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then cultured in adipogenic conditions (DMEM/10%FBS, 0.5 mM 3-isobutyl-1-methylxanthine, 1 μM dexamethasone (Sigma), and 10 μg/ml bovine insulin (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... blocked with 5% non-fat dry milk in TBS-0.1% Tween-20 for 1 h and incubated overnight with mouse anti-ataxin-3 (1:1000; Millipore), mouse anti-HA (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... On selected sections, immunoreactivities were tested: SATB1 (mouse, 1:500, SantaCruz) and GluR 2/3 (rabbit, 1:500, Millipore) were diluted in 0.1 M PB and incubated overnight at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) in a 1:3 volume ratio for recombinant enterokinase digestion (Novagen, 1 unit/ 50 μg protein) overnight (Gasparian et al. ...
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bioRxiv - Biophysics 2022Quote: ... Concentrated Cav1.2(ΔC)/Cavβ3/Cavα2δ-1 sample was immediately incubated with gabapentin (final concentration 2 mg ml-1, 11.7 mM) (Sigma-Aldrich) on ice for four hours prior to cryo-EM sample preparation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... GATA 3 (SAB2100898) (rabbit) 1:400 dilution for zebrafish tissue and 1:100 dilution for human tissue (Sigma Aldrich) and and CD44 (SAB1405590 ...
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bioRxiv - Bioengineering 2023Quote: ... Nuclei were stained at room temperature for 3 minutes with DAPI (Sigma Aldrich, D9542, 1:500, 1 mg/ml). All samples were washed with PBS and covered with aluminium foil until imaging.
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bioRxiv - Bioengineering 2023Quote: ... Nuclei were stained at room temperature for 3 minutes with DAPI (Sigma Aldrich, D9542, 1:500, 1 mg/ml).
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibodies (anti-TDP-43 1:750, PTGLabs; anti-SUMO2/3 [8A2] 1:500 Sigma) diluted in blocking buffer (1.5% Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... and the chocolate-flavored pellets were replaced with cotton pads infused with 1 ml of a 1% solution of 2,3,5-trimethyl-3-thiazoline (TMT, Sigma Aldrich) in water ...
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bioRxiv - Developmental Biology 2024Quote: ... The embryos were rinsed and permeabilized in PBS-0.1% triton, 3 times 30 min, and incubated in blocking solution (PBS-0.1% triton, 1% donkey serum (D9663, Sigma)) prior to incubating with primary and secondary antibodies ...
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bioRxiv - Bioengineering 2022Quote: ... crosslinking using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Carl Roth GmbH + Co. KG) and N-hydroxysuccinimide (NHS) (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) was applied ...
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bioRxiv - Cell Biology 2020Quote: ... then rinsed 3 times in PBS (5 minutes each) and embedded in 4% low gelling temperature agarose in PBS (Sigma-Aldrich A0701). 55 μm slices of the ovaries were cut with a vibratome (Leica VT 1000 S ...
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bioRxiv - Cell Biology 2020Quote: ... washed once with 5 mL ice-cold NETN250 buffer and lysed for 15 min at room temperature (RT) in ice-cold NETN250 buffer supplemented 5 mM MgCl2 and 125 U/mL benzonase (Novagen 71206-3). After addition of EDTA and EGTA to respective final concentrations of 5 mM and 2 mM to inactivate the benzonase and centrifugation for 30 min at 16,100 × g ...
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bioRxiv - Genetics 2019Quote: ... 59 nt spacer oligomers containing the 20 nt “spacer” and 19 nucleotides (5’ upstream, overlap to plasmid) and 20 nucleotides (3’ downstream, overlap to scaffold) were ordered (Sigma, Darmstadt, Germany). The different Cas9 vectors were linearized with restriction enzyme Acc65I ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed at 4°C for 3 h using 5 µg of an anti-FLAG M2 antibody (Sigma-Aldrich, F1804, mouse). For RIP-Seq ...
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bioRxiv - Genomics 2021Quote: ... The tissue was mechanically lysed with a scalpel in a total volume of 5 ml lysis buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Molecular Biology 2020Quote: ... The bound probe was visualized using 5-bromo-4-chloro-3 indoyl phosphate (NBT/BCIP; Sigma-Aldrich Corp., St. Louis, MO, USA). Bright field images were captured on the A1+ Nikon confocal microscope fitted with a DS-Ri1-U3 color camera at 20x magnification and 4076×3116 pixel resolution ...
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bioRxiv - Cancer Biology 2020Quote: ... It was mechanically dissociated using a scalpel in 1 ml of lysis buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclei were re-suspended in 5 ml washing buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed by sterile phosphate buffer saline (PBS) 3 times and then incubated with 5 mg/mL MTT (Sigma-Aldrich; #M2128) for 4 h ...
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bioRxiv - Molecular Biology 2020Quote: ... permeabilized with 0.5% (v/v) Triton X for 10 minutes and blocked for 30 minutes with PBS containing 3% goat serum (Sigma-Aldrich®), 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... permeabilized with 0.5% (v/v) Triton X for 10 minutes and blocked for 30 minutes with PBS containing 3% goat serum (Sigma-Aldrich®), 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2020Quote: ... Detection was performed by adding 200 μl of western blue substrate (SIGMAFAST™ BCIP®/NBT = 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium, Sigma) followed by incubation at room temperature in a dark and moist environment until the reagent’s color became visible ...
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bioRxiv - Microbiology 2020Quote: ... Primary human epithelial cells seeded on 10 cm2 dishes were cultured in BEGM medium and transduced with lentiviral particles harboring shRNA against KLK13 (a set of 3) or GFP gene in the presence of polybrene (5 µg/ml; Sigma-Aldrich, Poland). Cells were further cultured for 72 h at 37°C with 5% CO2 and positively transduced cells were selected using puromycin (5 µg/ml Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... cells were first washed once with phosphate-buffered saline (PBS) and supplemented with equilibrated (37 °C and 5% CO2) medium containing 500 μM indole-3-acetic acid (Sigma-Aldrich, I5148) freshly prepared before use ...
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bioRxiv - Bioengineering 2022Quote: ... cell-seeded wells (n=3 per group) were rinsed with PBS and incubated in 500 μL of 5% trichloroacetic acid (TCA, Sigma-Aldrich, T6399) in UPW for 30 minutes ...