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Citations for Millipore Sigma :
3051 - 3100 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Antibiotics used in protein expression and other analytical grade chemicals were purchased from Sigma. Unfractionated heparin was purchased from Sigma as a sodium salt ...
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bioRxiv - Biochemistry 2020Quote: ... FphF protein was initially purified by Ni2+ affinity chromatography (HIS-Select resin, Sigma-Aldrich) using an elution buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... were transiently transfected with the S protein expression construct using polyethylenimine (PEI; Sigma-Aldrich) in FreeStyleMedia (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... Mass inaccuracies were corrected using ProteoMass Peptide and Protein MALDI-MS calibration standard (Sigma). Glycan compositions were manually inspected using MALDI LIFT-TOF/TOF MS/MS.
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bioRxiv - Biochemistry 2020Quote: ... albumin (66 kDa) and carbonic anhydrase (29 kDa) protein molecular weights standards (Sigma Aldrich). Corresponding fractions for each chromatogram between 12 and 18 ml with an interval of 0.5ml were analyzed by SDS-PAGE (Biorad ...
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bioRxiv - Biochemistry 2020Quote: ... Protein visualization was achieved by using Coomassie Brilliant Blue G250 staining (Sigma-Aldrich, Germany) and imaging with a ChemDoc MP Imaging System (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... The proteins were concentrated using Amicon centrifugal filters (5k MWCO or 10k MWCO, Millipore) and purified further by size exclusion chromatography (Superdex 75 Increase ...
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bioRxiv - Neuroscience 2021Quote: ... and those directed against the following proteins: Actin (rabbit, Sigma, A2066, WB 1 : 2000), GFAP (mouse ...
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bioRxiv - Cell Biology 2021Quote: Protein was isolated from cells using 1X RIPA lysis buffer (EMD Millipore, Burlington, MA) containing 1X protease inhibitor cocktail (Thermo-Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were digested with lysyl endopeptidase (Wako Pure Chemical Industries) and trypsin (Sigma-Aldrich) with an enzyme:substrate ratio of 1:75 at 37 °C for 16 h ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing cleaved proteins was concentrated using a 100K MWCO concentrator (EMD Millipore) to 500 µL and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Cell Biology 2021Quote: ... were coated with 5 µg/mL human plasma fibronectin purified protein (EMD Millipore Corporation). Cells were seeded at a density of 315,000 cells per insert in 250 µL HITES media ...
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bioRxiv - Cancer Biology 2020Quote: Protein was extracted from tissue or cells by addition of RIPA Buffer (Sigma Aldrich) including complete Protease Inhibitor Cocktail Tablets (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein lysates were run on SDS-PAGE gels and transferred to PVDF membranes (Millipore). Membranes were blocked and probed with antibodies directed against RAS (RRID:AB_397425 ...
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bioRxiv - Cell Biology 2021Quote: ... the protein sample is transferred to the polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, USA). Blocking the membrane with 5% BSA (Bovine serum albumin) ...
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bioRxiv - Pathology 2021Quote: ... Immunoprecipitated proteins were separated on acrylamide-SDS gels and transferred to PVDF membranes (Millipore). The membranes were probed with rabbit anti SLP2 and mouse anti Flag M2 ...
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bioRxiv - Plant Biology 2021Quote: ... an equal amount of total protein extracts was incubated with anti-GFP (Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The pellet was dried and resuspended in Protein Extraction Reagent (C0856, Sigma-Aldrich®) supplemented with 1% Protease inhibitor (P9599 ...
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bioRxiv - Cell Biology 2021Quote: ... was conjugated with Atto-647N using a protein labeling kit (cat # 76508 Sigma-Aldrich). PDMS stamps were incubated with solution containing 50μg/ml fibronectin and 1μg/ml of the conjugated fibronectin in Dulbecco’s phosphate-buffered saline (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... and the fractionated proteins detected by direct staining of the gel with InstantBlue (Sigma) or by western blot ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were digested on-bead with 1 µg of trypsin (Sigma Aldrich, Cat# 6567) in 50 µL ABC buffer with rotation at 37℃ overnight ...
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bioRxiv - Systems Biology 2020Quote: Proteins from each sample were subjected to reduction using 5 mM DTT (Sigma-Aldrich) at 60°C for 20 min followed by alkylation using 10 mM iodoacetamide (Sigma-Aldrich ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... The equal amount of proteins were incubated with streptavidin-agarose slurry (Millipore, #16-126) at 4°C for 16 h in order to pull down all biotin-labeled proteins ...
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bioRxiv - Microbiology 2021Quote: ... proteins were transferred to Immobilon-PSQ PVDF membrane (0.2 μm pore size, Millipore Sigma) by electrophoresis at 100 V for 30 minutes in Tris-Glycine/Methanol buffer ...
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bioRxiv - Microbiology 2021Quote: ... followed by membrane protein cross-linking with 3,3’-dithiobis(sulfosuccinimidyl propionate) (Sigma; 1 mM), at low temperature ...
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bioRxiv - Microbiology 2021Quote: ... the Flag- or HA-tagged proteins were eluted with Flag peptide (Sigma, cat# F4799) or HA peptide (Sigma ...
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bioRxiv - Physiology 2021Quote: ... using the following primary antibodies: glial fibrillary acidic protein or anti-GFAP (Millipore, MAB360). Dako Animal Research Kit for mouse primary antibodies (Dako Diagnóstico S.A. ...
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bioRxiv - Microbiology 2021Quote: ... Digested proteins were analyzed by Western blotting with anti-M2 FLAG monoclonal antibody (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed and proteins were detected with Immobilon Western chemiluminescent HRP substrate (Millipore) using the ChemiDoc XRS+ Imaging System.
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bioRxiv - Cancer Biology 2020Quote: ... Protein turnover was analyzed by addition of Emetine (100 μM, Sigma Aldrich, Cat# E2375) and RNA decay by addition of Actinomycin D (5μM ...
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bioRxiv - Cancer Biology 2020Quote: RIP assays employed the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated in polyacrylamide gel and then transferred to polyvinylidene difluoride membranes (Millipore). These membranes were incubated with primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: Extraction of protein from isolated exosomes was performed using SDS2.5%/8M urea (Sigma-Aldrich) lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing cleaved proteins were concentrated using a 50K MWCO concentrator (EMD Millipore) to 1 mL ...
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bioRxiv - Cancer Biology 2022Quote: ... and antibody/bead complexes isolated with Magna ChIPTM Protein A+G magnetic beads (Millipore). Complexes were washed ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 30 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was first purified by immobilized metal-affinity chromatography (IMAC) using Ni2+ resin (Sigma), in a buffer consisting of 50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... The protein was concentrated using an Amicon centrifugal concentrator (EMD Millipore, Burlington, MA, USA) and then used for intasome assembly or flash frozen in liquid nitrogen and stored at -80°C ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were separated by SDS-PAGE and were transferred to PVDF membranes (Millipore; IPVH00010). After blocking with 5 % skim milk ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 100 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 30 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Purified proteins were concentrated using Amicon Ultra® 10,000 MWCO centrifugal filter units (Millipore) or Microsep™ Advance 3kDa (PALL ...
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bioRxiv - Biochemistry 2022Quote: ... Immunoreactive proteins were visualized using an enhanced chemiluminescence detection system (Millipore, Bedford, MA, USA). The relative intensity of the blot was measured using the ImageJ software and was normalized by the intensity of β-actin blot.
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples were subjected to SDS-PAGE and transferred to Immobilon-P membranes (Millipore). Membranes were immunoblotted with the indicated antibodies and developed with ECL (Thermo Scientific) ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were reduced by addition of 5 mM DTT (Merck Sigma, Cat. No. 43815) or 5 mM TCEP (Merck Sigma ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were loaded to a filter (Merck-Millipore, Microcon 10 kDa, Cat. No. MRCPRT010) after bead beating and reduced by addition of DTT and alkylated with iodoacetamide ...
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bioRxiv - Developmental Biology 2022Quote: ... The collected CM was concentrated by protein precipitation with 80%-saturated ammonium sulfate (Sigma) and dissolved in phosphate-buffered saline without calcium and magnesium [PBS (-)] supplemented with 0.1% Triton X-100 and a proteinase inhibitor mixture [0.2 mM 4-(2-aminoethyl ...
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bioRxiv - Microbiology 2022Quote: ... the respective proteins were overexpressed in BL21 DE3 cells carrying a pET28a vector (Novagen) with an (indirectly ...
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bioRxiv - Developmental Biology 2022Quote: ... The collected CM was concentrated by protein precipitation with 80%-saturated ammonium sulfate (Sigma) and dissolved in 50 mM Tris-HCl (pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... total protein lysates were instead incubated overnight with Anti-FLAG M2 Magnetic Beads (Sigma) or G-coupled magnetic beads ...