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Citations for Millipore Sigma :
3301 - 3350 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (EMD Millipore, catalog no. WBKLS0500) and ChemiDoc Imaging System (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were reduced using 5 mM tris (2-carboxyethyl) phophine hydrochloride (TCEP) (Sigma; 75259) for 10 min at 95° C and alkylated using 10 mM 2-iodoacetamide (Sigma ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... The eluted proteins were further concentrated using Amicon® Ultra-4 centrifugal filter (Millipore) and contaminants were removed using Gel-filtration chromatography ...
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bioRxiv - Neuroscience 2024Quote: ... 100 µg of proteins were reduced with 10 mM dithiothreitol (Sigma-Aldrich, MO, USA) at 37 °C for 60 min ...
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bioRxiv - Neuroscience 2024Quote: Proteins was isolated from cultured cells or brain tissues with RIPA buffer (Sigma, R0278) including protease inhibitors cocktails ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were eluted with wash buffer containing 1.5% β-mercaptoethanol (Sigma-Aldrich, M3148) at 37 °C for 0.5 h with agitation (350 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins on the membranes were detected by enhanced chemiluminescence reagents (WBKLS0500, Millipore, USA).
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bioRxiv - Genetics 2024Quote: ... Then the resolved proteins were transferred to 0.45 μm PVDF membranes (Merck Millipore, IPVH00010). The membranes were blocked ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto 0.45 nm pore polyvinylidene fluoride (PVDF) membrane (Millipore, USA) using an eBlot™ L1 wet transfer (GenScript Biotech ...
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred to a 0.2 μm polyvinylidene fluoride membrane (PVDF, Millipore, Billerica, USA) using a Bio-Rad semi-dry instrument ...
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bioRxiv - Immunology 2024Quote: ... protein bands on the blots were developed with chromogenic substrate SigmaFast BCIP/NBT (Sigma). All blots were run at least twice to check for reproducibility ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected with an anti-FLAG antibody (1:500, Sigma F1804). Samples were also stained with DAPI (300 nM ...
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bioRxiv - Microbiology 2024Quote: Recombinant ϕLf-UK PSB15 protein was expressed in B834 (DE3) pLysS cells (EMD Millipore) by transforming pR-PSB15 ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was eluted with 100 µL of 1.5 mg/mL FLAG peptide (Millipore) resuspended in wash buffer ...
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bioRxiv - Genetics 2023Quote: ... 20 μL Magna ChIP™ Protein A+G Magnetic Beads (Sigma-Aldrich 16-663) was washed four times with 1X DPBS supplemented with 5 mg/mL BSA and subsequently crosslinked to 5 μg antibody (Anti-FOXA1 antibody ...
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bioRxiv - Genetics 2023Quote: ... and the supernatants were precleared with 10 μL of Protein A-coated agarose (Millipore). The precleared supernatants were then divided into two equal parts and incubated with 20 μL 14-3-3 polyclonal antibody and IgG overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated using a 50 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... protein lysates were extracted from BMDMs using Radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich, R0278) complimented with protease inhibitor (Roche Diagnostics ...
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bioRxiv - Genomics 2023Quote: ... the supernatants were collected and protein concentration was measured by Bradford assay (Sigma-Aldrich). Extracts were boiled in presence of SDS loading buffer and analyzed by Western blotting.
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bioRxiv - Evolutionary Biology 2024Quote: ... The crude protein lysate concentration was quantified by bovine serum albumin (BSA) (Sigma-Aldrich) and 25 μg crude extract was loaded on NuPAGE™ 3-8% Tris-Acetate gels (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were concentrated using 10 kDa cut off Amicon centrifugal filters (Millipore, USA).
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bioRxiv - Bioengineering 2024Quote: ... Proteins were concentrated by centrifugation through Amicon Ultra 15 filter units (Millipore, Burlington, MA) with a 30-kD cutoff and then dialyzed against 3×PBS ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested overnight at 37C with trypsin (1:50 µg/µg) (Sigma-Aldrich) and lysyl endopeptidase (1:75 µg/µg ...
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bioRxiv - Microbiology 2023Quote: ... Protein molecular weights were estimated from elution volumes using BSA (66 kDa) (Sigma-Aldrich) and ovalbumin (45 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... and glial fibrillary acidic protein (mouse anti-GFAP, 1:400, Sigma-Aldrich, cat. #: G3893), and cell nuclei were counterstained using Hoechst (10 µg/mL) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were eluted in lysis buffer supplemented with 5% fresh β-mercaptoethanol (Sigma, M3148) and 1X SDS-PAGE loading buffer (5X SDS-PAGE loading buffer ...
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bioRxiv - Immunology 2022Quote: ... Proteins were reduced with the addition of 3 μL of 250 mM dithiothreitol (Sigma) at 37°C for 45 min and alkylated with 4.5 μL of 500 mM chloroacetamide for 30 min at room temperature in the dark ...
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bioRxiv - Immunology 2023Quote: ... The boiled proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Millipore). The membranes were blocked in TBST (Tris-buffered saline ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM TCEP and mixed with 1:5000 SYPRO Orange Protein Gel Stain (Sigma). 50 μL of assay mixture was dispensed per well in a white bottom qPCR plate and each condition was performed in technical triplicate ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was filtered through a 0.45μm ultra-low protein binding filter (Merck Millipore). Aliquots were stored at −80 °C.
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 minutes at 60°C with 10 mM dithiothreitol (Sigma) in 25 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... the separated proteins were transferred onto a methanol activated 0.45 μM PVDF membrane (Millipore) at 70V for 120 minutes at 4 °C in Transfer Buffer (192 mM Glycine ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were detected with a streptavidin-HRP conjugate (Millipore-Sigma, Cat#: OR03L-200UG), used at 1:2,000 dilution (see Reagent Table).
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bioRxiv - Molecular Biology 2023Quote: ... The remaining protein was run through a 50 kDA cut off spin concentrator (Millipore) and dialyzed into 2X storage buffer (48 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... The M-protein solutions were diluted 1:1 with 8 M Urea (Sigma Aldrich) in Tris.HCl (pH=7 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted in two column volumes of 0.2 mg/ml 3xFLAG peptide (Sigma) and dialyzed against 50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were concentrated to >10 mg/mL using Amicon Ultra-15 Centrifugal Filters (Millipore). For some of the mutants assayed in this study ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins separated by SDS-PAGE were transferred to PVDF membranes (Millipore, Cat#PVH00010). After blocking with 5% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were then wet transferred to a polyvinylidene difluoride (PVDF) membrane (IPVH00010, Sigma Aldrich). The membrane was incubated in blocking buffer consisting of 5% milk in PBS plus 0.1% Tween-20 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then reduced by 10 mM tris(2-carboxyethyl)phosphine (TCEP, Millipore Sigma) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: Total proteins were extracted from organoid cell pellets using 1X RIPA buffer (Sigma Aldrich) supplemented with 1X Halt™ protease and phosphatase inhibitor cocktail (Pierce) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were concentrated using AmiconUltra (M.W. 3,000 Da; Sigma-Aldrich, St. Louis, MO, USA) and stored at −80 °C before use.
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore, Massachusetts, USA) and the membranes were blocked with PBS containing 5% non-fat dry milk for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein was then transferred to 0.4 μm polyvinylidene fluoride (PVDF, Merck Millipore) membrane ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by 10% SDS-PAGE gel and transferred onto PVDF membranes (Millipore). After blocked in 5% skim milk for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were filtrated using a 96-well filter plate (Millipore, Burlington, MA, USA) to remove precipitated protein and the A280 was remeasured before starting the binding assay ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were normalized with Milliplex® MAP Assay Buffer (EMD Millipore, Billerica, MA) to 6 μg protein per 12.5 μL for cytokine analysis and 1 μg protein per 12.5 μL for the MAPK pathway analysis ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with antibodies against OL markers proteins including Olig2 (MABN50, 1:500 Millipore, CA ...
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bioRxiv - Neuroscience 2023Quote: Total protein from cortical organoids and HLCs were isolated using RIPA buffer (Sigma-Aldrich) consisting of protease and phosphatase inhibitors (Roche ...