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Citations for Millipore Sigma :
2951 - 3000 of 3014 citations for 6 bromochromone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Rescue of cholesterol accumulation were performed by adding the drugs to cultures previously incubated overnight with Bodipy-cholesterol and further incubated for 6 h at the following concentrations: Evodiamine (Sigma, stock 1000x in DMSO) 10 µM ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then centrifuged at 4,000 rpm for 22 min at 4°C and the supernatants were transferred to another set of 6 eppendorf tubes containing a mixture of pre-washed beads (Millipore Sigma, GE17-0886-01) and 12.5 uL (12.5 ug ...
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bioRxiv - Microbiology 2023Quote: ... The extracts were diluted from 1:10-3 to 1:10-6 in 10 mM MgCl2 for plating so that spreading of 50 μl extract with a delta cell spreader (Sigma-Aldrich, St. Louis, USA) resulted in a density of 100-300 CFU on square plates (12 x 12 cm ...
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bioRxiv - Physiology 2023Quote: Hepatocytes were isolated from C57BL/6 and SIRT4 KO mice (8–12 weeks old) by perfusing the liver tissue with collagenase type IV (Sigma-Aldrich, catalog no. C5138) containing media as described in Shaw et al ...
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bioRxiv - Immunology 2023Quote: ... 300,000 WT T84 cells per well in a 6-well plate were treated with 20 µL lentivirus using 3 µL Polybrene transfection reagent (Sigma Aldrich #TR-1003-G) diluted in 3 mL media ...
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bioRxiv - Neuroscience 2023Quote: ... Serial sections were placed on glass slides (Matsunami, Osaka, Japan) and stained with 4’,6-diamidino-2-phenylindole (DAPI; D9542, Sigma-Aldrich, St. Louis, MO) for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells (5 × 104 cells/cm2) were grown in triplicate in 24-well plates pre-coated with 6 μg fibronectin (Sigma-Aldrich catalog number FC010) and transfected the next day with 500 ng pRL0381 or pRL0385 with TransIT-LT1 ...
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bioRxiv - Microbiology 2023Quote: ... the media was replaced and supplemented with 200 ng/mL aTc and either DMSO or 6 μg/mL brefeldin A (Sigma-Aldrich catalog number B6542). After an additional 16 h ...
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bioRxiv - Immunology 2023Quote: ... bone marrow cells were obtained from femurs and tibiae of WT C57BL/6 mice by flushing with complete RPMI 1640 media containing 10% heat inactivated fetal bovine serum (FBS) (Sigma-Aldrich, non-USA origin). Red blood cells were lysed using ACK lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... or 6 days (long-term) with or without a cytokine mix of recombinant human IL-1β (R&D Systems, USA or Sigma-Alrich, USA), recombinant human IFN-γ (PeproTech ...
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bioRxiv - Cancer Biology 2024Quote: ... neurospheres were collected or attached to standard culture plates in media containing 5% FBS for 6 h and stained with crystal violet solution (Sigma-Aldrich, 548-62-9).
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bioRxiv - Neuroscience 2024Quote: ... Unbound antibody was washed with PBS++ including 4’,6-diamidino-2-phenylindole for initialization (DAPI; 1:10 000, Sigma Aldrich, St. Louis, MS, USA). Where needed ...
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bioRxiv - Plant Biology 2019Quote: ... AGM was supplemented with vitamins (Duchefa M0409), cytokinin (200 nM 6-Benzylaminopurine), and IAA (3-indole acetic acid, 1 mM) and/or Trichostatin A (TSA, Sigma, T8552, final concentration 5 µM) or mock before pouring ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed with Tris-buffered saline/Tween 20 and then incubated with an anti-acetyl-α-tubulin (SIGMA-ALDRICH #T7451 Clone 6-11B-1), α-tubulin (SIGMA-ALDRICH #T9026) ...
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bioRxiv - Bioengineering 2021Quote: ... was used to stain the sections for EdU and 4′,6-diamidino-2-phenylindole (DAPI; #D9542; 1:1000 dilution; Sigma-Aldrich, St. Louis, MO, USA). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... the target cells were trypsinized and replated in 6-cm dishes in media containing 2 μg/mL Puromycin (P8833, Sigma-Aldrich Quimica S.A., Madrid, Spain). Selection media was changed every 48-72 h for a minimum of 1 week before cell lines were frozen in 50% FBS and 10% DMSO and stored in liquid nitrogen for later use.
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bioRxiv - Molecular Biology 2020Quote: ... The media was then carefully removed from the 10 cm dish and the enriched myoblasts population was seeded in a 6 well-plate coated with Matrigel (Sigma-Aldrich, St. Louis, MO, USA) in plating media ...
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bioRxiv - Molecular Biology 2022Quote: Antibodies that recognize the following proteins and epitope tags were used: glucose-6-phosphate dehydrogenase (G6PDH; Sigma-Aldrich A-9521, 1:20,000 or 1:50,000), H2B (Active motif 39237 ...
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bioRxiv - Microbiology 2020Quote: Confluent cell monolayers grown in 96-well plates were pretreated with a range of concentrations of brefeldin A (BFA, EMD Millipore CAS 20350-15-6) or Golgicide A (GCA ...
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bioRxiv - Neuroscience 2021Quote: ... sample from the overnight cleavage step was concentrated to ∼5 mg/ml and injected into the Superose 6 Increase column equilibrated in SEC buffer with 1 mM DEET (Sigma Aldrich, CAS#134-62-3). Peak fractions were concentrated to ∼5 mg/ml and used immediately for grid preparation.
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CSF1R inhibition by a small molecule inhibitor affects hematopoiesis and the function of macrophagesbioRxiv - Neuroscience 2020Quote: BMDM were primed with 150U/mL interferon-γ (IFNγ) for 6 hours followed by LPS at final concentration of 10ng/mL (Sigma-Aldrich, St. Louis, MO) added into culture medium for 20 hours ...
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bioRxiv - Genomics 2021Quote: HEK293T cells (ATCC® CRL-3216™) were seeded at a density of 5×105 cells/well in a 6-well culture plate (Sigma Aldrich, cat # CLS3516). The following day ...
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bioRxiv - Neuroscience 2020Quote: ... and IL-6 in the conditioned media of WT and Akt2 KO mouse primary microglia cultures treated with LPS (200ng/ml, Sigma-Aldrich, St. Louis, MO, USA) and IFNγ (20ng/ml ...
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bioRxiv - Pathology 2021Quote: ... were starved 6 hours from Zeitgeber 14 (ZT14) and refeed for 8 hours with the CD and 20% glucose (Sigma-Aldrich, Saint Louis, MO, USA) in the drinking water.
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bioRxiv - Evolutionary Biology 2021Quote: ... The total amount of glucose was determined photometrically at 340 nm in a 96-well microplate photometer (HR 7000, Hamilton, Reno, NE, USA) after enzymatic conversion to gluconate-6-phosphate (hexokinase reaction, hexokinase from Sigma Diagnostics, St. Louis, MO, USA). NSC (Non Structural Carbohydrates ...
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bioRxiv - Neuroscience 2022Quote: ... To assess either apoptotic and necrotic cell death pycnotic nuclei were identified with 4’,6-diamidino-2-phenylindole (DAPI) staining (Cat# D9542-5MG, Sigma-Aldrich, St Louis, MO, USA).
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bioRxiv - Microbiology 2022Quote: ... quenched and 6-Carboxyfluorescein-filled liposomes were extruded through Whatman® Nuclepore™ Track-Etched Membrane (pore size=200 nm) (Sigma Aldrich, Saint Louis, MO) as previously described(Jimah et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were infected 12.5 µL of concentrated lentivirus pTRE-SadCas9-2xKRAB-dtTomato and 12.5 µL of concentrated lentivirus pCMV-rtTA in 400 µL of StemFlex medium supplemented with 6 ug/mL of polybrene (Millipore, Cat. No. TR-1003-G). After 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... BSA (1%) and PBS-T (0.5%) followed by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI) in PBS (1x) (Sigma-Aldrich, St. Louis, MO, 1:1000) for 20 minutes at RT ...
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bioRxiv - Neuroscience 2024Quote: Sensitivity to cold was assessed immediately following testing for mechanical paw withdrawal thresholds by applying a droplet of acetone (Sigma-Aldrich; approximately 5-6 μL) from the end of a blunt one C.C ...
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bioRxiv - Cell Biology 2022Quote: ... FAM76B knockdown U937 cells were plated in 6-well plates (5×105 cells/well) and treated with 10 ng/ml PMA (Sigma-Aldrich, St. Louis, Missouri, USA) in association with 10 ng/ml LPS and 20 ng/ml hIFNγ (Sino Biological Inc. ...
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bioRxiv - Immunology 2023Quote: ... we removed the head kidneys from RNAlater and placed them in a tube with ∼10 ceramic beads (1.4 mm) and 600 µL of RTL Buffer enriched with 6 µL β-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, USA) to avoid sample degradation by RNases ...
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bioRxiv - Bioengineering 2023Quote: ... the nuclei were stained at room temperature for 15 minuts with 4’,6-diamidino-2-phenylindole (DAPI)(Sigma Aldrich, D9542, 1:500, 1 mg/ml) to yield a blue nucleic fluorescence by binding to nucleic acids.
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bioRxiv - Neuroscience 2023Quote: ... 10-15 neurospheres and 4-6 organoids per cell line were rinsed twice with PBS and then lysed in RIPA buffer (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... This solution contained 30 µl of scrambled or 10 µl VPS34-targeting shRNA per target sequence to a total of 30 µl and was supplemented with 6x polybrene to reach a final concentration of 6 µg / ml (Sigma Aldrich, Cat. # TR-1003-G) in a complete medium ...
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bioRxiv - Bioengineering 2023Quote: ... Tissue sections were then trimmed to a ∼ 6 mm x 6 mm region centered about the implant site and the tissue block was embedded in a 4% agarose gel (Sigma-Aldrich, Saint Louis, MO, USA). This tissue-gel block was glued onto the slicing platform of a vibratome (VT 1000S ...
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bioRxiv - Immunology 2024Quote: ... we used inhibitors of ERK1/2 (Trametinib, SelleckChem, Catalog # S2673), p38 (Losmapimod, Selleckchem Catalog #S7215), NF-kB (BAY11-7082, Catalog # S2913) and JNK inhibitor (Millipore-Sigma Catalog # CAS 129-56-6) 2 hr before stimulation with R848 or Poly I:C until sample collection at 8 or 24hrs for mRNA or protein levels of antiviral and inflammatory cytokines and chemokines.
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bioRxiv - Biophysics 2020Quote: A polyethylene glycol (PEG) solution (w/v, 6%) was made by dissolving PEG powder (Molecular weight 300,000; Sigma-Aldrich Co Ltd, MO, US; product of USA) in Milli-Q water at 50 °C with magnetic stirring for 8 h (25) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then blocked in 1% BSA for 20 minutes before being incubated in a primary antibody against acetylated α tubulin (1:4000 dilution, clone 6-11B-1, Sigma-Aldrich, St. Louis, MO, USA), GFP (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies used in this study were: anti-acetylated α-tubulin (Antibody registry ID: AB_477585, clone 6-11B-1, mouse IgG2b, Sigma, diluted 1:250 in blocking solution), anti-SV2 (AB_2315387 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... They were then placed under isoflurane anesthesia (1.5% in 30% oxygen and 70% nitrous oxide) in a stereotaxic apparatus (David KOPF Instruments, Tujunga, CA, USA) and received injection of 6-OHDA (Sigma-Aldrich Co., St. Louis, MO, USA) at four sites targeting the dorsomedial striatum bilaterally (AP ...
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO], 20 ng/ml bFGF [Sigma], and 20 ng/ml EGF [Sigma]) before collection for single-cell analysis ...
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bioRxiv - Plant Biology 2021Quote: The dried samples were derivatized for 2 hours at 37 °C in 50 μI of 20 mg ml-1 methoxyamine hydrochloride (Sigma-Aldrich, cat. no. 593-56-6) in pyridine followed by a 30 min treatment at 37 °C with 100 μI of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent ...
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bioRxiv - Microbiology 2021Quote: ... Bacillus cultures were adjusted to 2*10−2 or 8*10−4 at OD600 and 5-fold dilution series were prepared with 6 steps using peptone saline as diluent ([Millipore]; Maximum Recovery Diluent 9.5 g/L). 15 μl of each bacterial dilution was inoculated in consecutive wells by spotting the solution in the center of the wells ...
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bioRxiv - Immunology 2020Quote: Perigonadal white adipose tissue (WAT) was digested in 6 ml of Collagenase II buffer (Collagenase II 2mg/ml; Sigma Aldrich, USA, 0.5 % BSA in 1X PBS) at 37 °C with shaking at 200 r.p.m for 20 min ...
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bioRxiv - Plant Biology 2022Quote: ... and the residue was derivatized for 120 min at 37 °C (in 40 μl of 20 mg ml−1 methoxyamine hydrochloride (Sigma-Aldrich, cat. no. 593-56-6) in pyridine followed by a 30 min treatment at 37 °C with 70 μl of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent ...
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bioRxiv - Neuroscience 2023Quote: ... animals received the first of 6 injections of either vehicle (propylene glycol) or progesterone (>99% progesterone, purified from rats, Sigma-Aldrich Co. St. Louis, MO, USA) at a dose of 4 mg/kg (Shear et al. ...
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bioRxiv - Physiology 2023Quote: Intracellular succinate concentration was measured in cells under normoxic conditions and after exposure to 1 and 6 h hypoxia using a commercial colorimetric assay (Sigma catalog # MAK184, St. Louis, MO, USA) according to manufacturer instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and the residue was derivatized for 120 min at 37°C (in 40 μl of 20 mg ml-1 methoxyamine hydrochloride (Sigma-Aldrich, cat. no. 593-56-6) in pyridine followed by a 30 min treatment at 37°C with 70 μl of N-methyl-N- (trimethylsilyl)trifluoroacetamide (MSTFA reagent ...
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bioRxiv - Biophysics 2023Quote: Cell adhesion on collagen fibers (Fig. 7a -d) is prevented either by adding pluronic surfactant (1 vol% in PBS, Sigma-Aldrich, cat. no. 9003-11-6), or by calcium ion chelation with 5 mM ethylenediaminetetraacetic acid (EDTA ...