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Citations for Millipore Sigma :
2701 - 2750 of 3014 citations for 6 bromochromone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The gradients were then displaced into a UA-6 continuous UV detector (Teledyne ISCO) using a syringe pump (Brandel) containing Fluorinert FC-40 (Sigma #F9755) at a speed of 0.75 ml/min ...
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bioRxiv - Neuroscience 2023Quote: ... was induced in 7–10-week-old C57BL/6 or transgenic mice by hourly IP injections of cerulein (50 μg/kg; Cat. #: C9026 Sigma-Aldrich) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 h at room temperature simultaneously with 4′,6-diamidino-2-phenylindole (DAPI: 1:2500, D9542; Sigma-Aldrich-Aldrich, USA) and Phalloidin Alexa488 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated on 6-well plates pre-coated with poly-L-lysine (10 µg/mL, Sigma-Aldrich, St. Louis, MO) and cultivated in DMEM medium containing 10% FCS and 2 mM L-glutamine at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
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bioRxiv - Systems Biology 2023Quote: ... 6 M Urea and 1% Sodium Dodecyl Sulphate supplemented with 100mM phenylmethylsulfonyl fluoride and 1% phosphatase inhibitor cocktail 3 (Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then centrifuged at 200 rcf for 5 min and resuspended at a density of 2×105 cells/ml in ultra-low attachment 6-well plates (Sigma, CLS3471) using N2B27 medium (50% Advanced DMEM/F12 (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... and postfixed with 4% cold PFA for 10 min followed by permeabilization in 0.5% Triton X-100/PBS for 3 h at room temperature (20–25°C) and incubation with 2 µg ml-1 4′,6-diamino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 25 mg of minced tissue was digested on ice for 4 minutes in 1 ml of Collagenase/Elastase/Dispase enzyme mix (6 mg/ml Collagenase A (Sigma, 10103578001), 4.3 units/ml Elastase (Worthington ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were cultured in feeder-free conditions on tissue-culture treated 6-well plates (Falcon® 353046) coated with 0.1% gelatine (Sigma G1393), in KnockOut DMEM (Gibco™ 10829018 ...
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bioRxiv - Neuroscience 2023Quote: ... 6-well plates in maintenance media (base culture media further supplemented with 150 µM ascorbic acid (AA, Sigma, cat. no A4544), 3 µM CHIR-99021 (Axon Medchem ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was washed and disrupted with acetone two times and re-dissolved in 200 mM ammonium bicarbonate with 6 M Urea (Sigma Aldrich). Human serum albumin (0.1 µg ...
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bioRxiv - Neuroscience 2024Quote: Mouse vibrissae were carefully isolated from the upper lip pad of 4-week-old C57BL/6 male mice and cultured in Williams E medium (Sigma-Aldrich), supplemented with 1% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were rinsed in PBS followed by a nuclear staining with DAPI (4’,6-diamidino-2-fenylindool, 1:1000 in PBS, 32670, Sigma-Aldrich). All sections were mounted with Mowiol and a glass cover slide ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche), and the cells were fixed 5–6 days later ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were rinsed with PBS and stained according to manufacturer’s protocol (Millipore #BG-6-B, #BG-7-B, #BG-8-C). Embryos were then fixed again in 4% PFA (in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... were washed two times with PBS and lysed by scraping into 500 μl per plate of lysis buffer (40 mM Tris/HCl pH 7.6, 8 M urea, EDTA-free protease inhibitor complete mini [Roche], and phosphatase inhibitor cocktails 1, 2, and 3 [Sigma-Aldrich] at 1× final concentration according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... two serial sections) or TILNTd (TILNTd: two serial sections) were subjected to antigen retrieval procedures with citrate buffer pH 6 (Sigma Aldrich) at 100-112 °C for 15 minutes in a TintoRetriever Pressure Cooker (BioSB ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used in this study were as follows: mouse anti-Actub (1:1,000 or 1:2,000, Sigma, 6-11B-1), guinea pig anti-Ick (1:100 ...
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bioRxiv - Cell Biology 2024Quote: ... EBs at day 5 were transferred to a new 6-well plate coated with PDL (Millipore. Catalog. MILL-A-003-E)/Laminin (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... islets isolated from chow-fed C57BL/6 WT mice were pre-incubated in 20 mM cholesterol loaded onto methyl-β-cyclodextrin (MβCD) (C4951, Sigma Aldrich) diluted in 1X Krebs-Ringer bicarbonate-Hepes (KRBH ...
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bioRxiv - Microbiology 2024Quote: ... The pooled lentivirus media was filtered through a 0.45 mm pore filter and used to transduce A549 cells in the presence of 6 μg/ml polybrene (Sigma-Aldrich, TR1003). Twenty-four hours later ...
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bioRxiv - Molecular Biology 2024Quote: ... The stained cells were mounted on a coverslip with a mounting medium containing 1 µg/mL 4ʹ,6-diamidino-2-phenylindole (DAPI) dilactate (Sigma, D9564) and 100 µg/mL p-phenylenediamine (PPD ...
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bioRxiv - Cell Biology 2024Quote: ... the center of the glass dish was coated with 6% Concanavalin A (Canavalia ensiformis Jack Bean, type IV Sigma C2010-G) for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded at a density of 0.5 × 106 on 6-well plates precoated with 50 μg/mL poly-L-ornithine (Sigma #P-3655) and 1 µg/mL fibronectin in DMEM F12 with 1 % N-2 supplement (Invitrogen #17502048) ...
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bioRxiv - Systems Biology 2024Quote: ... pre-coated with Poly-L-ornithine hydrobromide (6 µg/mL in 1xPBS, Sigma-Aldrich, for 1 hour at 37°C, SigmaAldrich) followed by Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane (1.2 mg/mL in 1xPBS for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2024Quote: Mouse head and vertebral column samples were placed in a 15 ml centrifuge tube filled with perfluoropolyether (Fomblin Y, LVAC 16/6, average molecular weight 2,700; Sigma□Aldrich, USA) (Massalimova et al ...
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bioRxiv - Neuroscience 2024Quote: ... the glutamatergic nature of EPSCs was confirmed by a complete block of spontaneous activity with 6-cyano-7-nitroquinoxaline-2,3-dione disodium salt (CNQX, 50 μM, Sigma-Aldridge, US) and D-2-amino-5-posphonopentanoic acid (APV ...
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bioRxiv - Immunology 2024Quote: ... Wells were then washed 6 times with PBS/T and developed with 100 µL of p-nitrophenyl phosphate substrate at 1 mg/mL (Sigma-Aldrich) and optical density read at 405 nm (OD405 ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then blocked in 6% donkey serum and immunolabeled for protein gene product 9.5 (PGP9.5, 1:500, Millipore-Sigma, SAB4503057-100UG) as a pan-neuronal marker or rabbit-IgG (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs (4.5·106 cells/mL) and MSCs (6·105 cells/mL) were suspended in EGM-2MV supplemented with thrombin (4 U/mL) (Sigma-Aldrich). A solution of fibrinogen (8 mg/mL ...
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A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: ... 6–8-week old B6D2F1 females were hormone primed by an intraperitoneal injection of pregnant mare serum gonadotropin (PMS, EMD Millipore) followed 46 hours later by an injection of human chorionic gonadotropin (hCG ...
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bioRxiv - Microbiology 2024Quote: ... LC-MS grade chemicals Ammonium formate (CAS 540-69-2) and formic acid (CAS 64-18-6) were from Sigma (Germany). Authentic metabolic standards like L-alanine ...
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bioRxiv - Cell Biology 2024Quote: Cells were cultured in 6-well plates with EGM-2 medium supplemented with 4% dextran (Sigma-Aldrich, St. Louis, MO, 31392). Hemodynamic flows were applied to using a 1o tapered stainless steel motorised cone (UMD-17 (Arcus20 Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 μL of each lysate (concentrations adjusted to 2.5 μg/μL) was treated with 6 μL of premixed click chemistry mixture (final concentrations of 100 μM biotin-N3 (Sigma-Aldrich, 762024), 1 mM CuSO4 ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
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bioRxiv - Developmental Biology 2021Quote: Recombination of the conditional allele was induced in Atxn10flox/flox;CAGG-creERT2 mice at 6 and 8 weeks old by a single intraperitoneal (IP) injection of 9mg tamoxifen (Millipore Sigma, T5648) per 40g (body weight ...
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bioRxiv - Molecular Biology 2021Quote: ... Apoptotic cell death was detected by annexin-V/propidium iodide staining and subsequent flow cytometry analysis using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Annexin-V-FITC emission was detected with the green filter at 525/530 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... washed and resuspended in PBS and measured by flow cytometry (FACS) using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Fluorescence was excited at 488nm and BODIPY emission was recorded at 530 and 585 nm ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22, CRP; Millipore Sigma - RAB0096-1KT). In brief ...
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Dopamine depletion can be predicted by the aperiodic component of subthalamic local field potentialsbioRxiv - Neuroscience 2021Quote: ... 4 or 8 μl of 6-OHDA (5 μg in 1 μl saline containing 0.1% ascorbic acid; Tocris, Bristol, ENG and Sigma-Aldrich, MA, USA) was injected into the left MFB at the following coordinates ...
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bioRxiv - Molecular Biology 2021Quote: ... A BioSpec bead-beater was used to lyse cell pellets corresponding to 3-6 OD600 units in the presence of 100 μL of acid washed beads and 300 μL of 20 % TCA (Sigma-Aldrich T6399). 2 x 3-minute pulses were used ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 x105 HEK293 cells were seeded in each well of a 6-well plate and were transfected with 2 μg of respective plasmid DNA using PEI reagent (Sigma-Aldrich, 408727). After 24 hours ...
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bioRxiv - Biochemistry 2019Quote: apoTaLPMO9A was prepared by dialyzing the purified TaLPMO9A extensively using D-tube Dialyzer Maxi (6-8 kDa) from Millipore (Billerica, MA, USA). Dialysis was performed in 20 mM phosphate buffer (pH 7 ...
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bioRxiv - Immunology 2019Quote: ... erythrocytes were depleted by sequentially layering 10 ml PBS followed by 8 ml of 6% w/v dextran (high molecular weight, Sigma-Aldrich, in PBS) and gently inverting ...
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bioRxiv - Genomics 2019Quote: ... and 12.5 ng/µL oligo(dT) at 42°C for 2 h in the presence of 6 ng/µL actinomycin D (Sigma, #A1410-2MG) to prevent antisense artifacts (Perocchi et al ...
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bioRxiv - Bioengineering 2020Quote: ... and 800 mg L-1 humic acids (CAS No. 1415-93-6) were prepared by adding in ultrapure water (Sigma Aldrich, UK).
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bioRxiv - Neuroscience 2020Quote: ... and MT+SNL mice from tissues collected on day 6 (post behavioral testing) using Tri Reagent (T9424, Sigma Aldrich, St. Louis, MO) and RNeasy Micro kit (74106 ...
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bioRxiv - Neuroscience 2021Quote: ... iPSC-derived neuron progenitors were expanded using 6-well plates coated with 15 μg/ml Poly-L-Ornithine (Millipore Sigma Cat# P4957) and 10 μg/ml laminin (Invitrogen Cat# 230171015 ...
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bioRxiv - Cell Biology 2021Quote: ... lentiviral particles were generated by transfecting HEK293T cells with 6 μg PLKO.1 scramble control and MISP-targeted shRNA plasmids (SIGMA; TRCN0000422523, TRCN0000116527), 4 μg psPAX2 packing plasmid (Addgene ...