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Citations for Millipore Sigma :
2801 - 2850 of 3014 citations for 6 bromochromone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and added dropwise to HEK293T cells that were seeded onto 10-cm dishes a day prior at a density of 5-6 × 106 cells in 10 ml of fresh DMEM with 10% heat inactivated FBS (Sigma-Aldrich #14009C). 18hrs after transfection ...
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bioRxiv - Microbiology 2022Quote: A CRISPR array containing 6 identical spacers targeting the tetR gene flanked by 7 repeats was cloned into pCDF-Duet (Novagen, Merck Millipore) by ligation after NcoI and SalI digestion ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated for two hours with Alexa Fluor® 647 labeled secondary antibody (1:500), and nuclei were labeled with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Sigma-Aldrich, #D8417). The fluorescent images were obtained with a Zeiss Axio Observer inverted microscope (40 × NA 1.3 oil objective ...
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bioRxiv - Physiology 2023Quote: ... mice were fasted for 6 hours followed by oral gavage of 150 µL of 80 mg/mL FITC-dextran (Sigma, 4 kDa). Blood was collected via tail vein at baseline and 1-hour post-gavage ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated on 6-well cell culture-treated plates and differentiated using 100 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, P1585) for 72 h in serum-free RPMI 1640 medium (SFM ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were washed several times in PBSDT and endogenous peroxidase activity was inactivated by treatment with 6%H2O2 (Sigma-Aldrich, Cat# H1009) for 1 hr at 4°C ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... the slide was washed three times with the wash buffer and stained sequentially with the Immunol Fluorence Staining Kit with Alexa Fluor 647-Labeled Goat Anti-Rabbit IgG (P0180, Beyotime, China) and 4’,6-diamidino-2-phenylindole (DAPI, MBD0015, Sigma-Aldrich, USA) as per the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... duttoni (NIH Rocky Mountain Laboratories, Hamilton, MT) were grown in BSK-H medium with 6% rabbit serum (Sigma Aldrich, St. Louis, MO) until the culture reached log phase (∼4 days) ...
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bioRxiv - Immunology 2024Quote: ... The minced tumor tissue was transferred to a 6-well plate containing 3 mL/well of tumor digestion media (NK cell media + 1 mg/mL collagenase IV (Sigma, cat. C5138) + 0.2 mg/mL DNAse I (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.05% Tween 20 (pH 6) at 98°C and/or treatment with bacterial type XXIV proteinase (10 μg/mL; Sigma; Catalog #P8038). The sections were blocked with 2% bovine serum albumin (VWR ...
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bioRxiv - Developmental Biology 2024Quote: ... tissue pieces (1 to 6 mm3) were equilibrated for ten minutes on ice in cryomedium consisting of 1.5M dimethylsulfoxide (DMSO, D2650, Sigma-Aldrich, Hoeilaart, Belgium), 0.15M sucrose (D21881000 ...
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bioRxiv - Cell Biology 2024Quote: ... Vessel patency and intravascular flow was confirmed between days 6-8 by addition of sterile 50µg/mL 70kDa FITC- or rhodamine-conjugated dextran (Sigma #FD70S or #R9379) to the media reservoirs ...
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bioRxiv - Immunology 2024Quote: ... cells were seeded in a 6-well plate and transduced with 10 μL of concentrated lentivirus in the presence of 10 μg/mL polybrene (Millipore-Sigma; H9268) and replaced with fresh media 24 hrs ...
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bioRxiv - Cell Biology 2023Quote: ... permeabilized for 4-6 h with 10% goat serum in PBS containing 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), and incubated overnight at 4°C in primary antibody in PBS containing 0.1% Tween 20 (PBS-Tw ...
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bioRxiv - Cell Biology 2023Quote: Recombination of the conditional tm1c allele was induced in utero by injecting time pregnant females with a single intraperitoneal (IP) injection of 6 mg tamoxifen (Millipore Sigma, T5648)/40 g body weight dissolved in corn oil ...
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine levels in PBMC culture supernatants were measured using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel-Custom 6 Plex-Immunology Multiplex Assay (Millipore; #HCYTOMAG-60K). Analytes included were the following ...
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bioRxiv - Neuroscience 2023Quote: ... Digested pancreata were washed with HBSS +0.1% BSA and subjected to discontinuous density gradient using histopaque (6:5 Histopaque 1119:1077; Sigma, St. Louis, MO). The islet layer (found at the interface ...
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bioRxiv - Cell Biology 2023Quote: ... Human dermal fibroblasts were isolated and grown from 6 mm skin punch biopsies as previously described.70 Dermal fibroblasts were cultured on 0.1% gelatin (Sigma catalog number: G1890) treated wells using fibroblast media consisting of DMEM (Gibco catalog number ...
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bioRxiv - Cell Biology 2022Quote: ... to block non-specific staining and incubated with a mouse anti-acetylated tubulin antibody (Clone 6-11b-1, 1:100; Sigma Cat# T6793) for 4 h at room temperature ...
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bioRxiv - Genomics 2022Quote: The stable cell lines constantly expressing the minigenes were subjected to four time points (0, 3, 6, and 9 h) of post actinomycin D treatment (final concentration of 1 μg/mL; Sigma, no. A9415) treatment in three biological replicates ...
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bioRxiv - Biochemistry 2023Quote: ... C2C12 cells were seeded in a 6 cm dish and 105-106 virus particles were added dropwise to media containing 8 ug/mL Sequabrene (Sigma S2667-1VL) and 25 mM Hepes buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... The murine melanoma cell line B16F10 derived from a C57BL/6 mouse (ATCC, cat# CRL-6475) was purchased from ATCC and grown in DMEM (Sigma, cat# D6546) supplemented with 10% Fetal Bovine Serum (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated 2 hr at 4°C with rotation after addition of either 6 μg mouse αSFPQ (Sigma-Aldrich WH0006421M2, RRID:AB_1843565)) or 6 μg normal mouse αIgG (Santa Cruz sc-2025 ...
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bioRxiv - Developmental Biology 2022Quote: ... T8787)/PBS at 37C for 2 days and subsequently immersed in a blocking solution of 10% DMSO + 6% donkey serum (EMD Millipore: S30) + 0.2% Triton X-100/PBS at 37C for 3 days ...
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bioRxiv - Systems Biology 2023Quote: ... K562 cells were transduced in 6-well plates by mixing the cells and virus with 1:500 dilution of 4 mg/mL Polybrene (Sigma H9268-5G). For spinfection ...
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bioRxiv - Immunology 2023Quote: ... were cocultured for 6 hours with autologous herpesvirus papio-immortalized B lymphoblastoid cell lines (B-LCLs) pulsed with Nef peptides (Sigma-Aldrich Japan) at 1 μM concentration or as indicated otherwise under GolgiStop (monensin ...
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bioRxiv - Developmental Biology 2023Quote: A feeder layer of inactivated murine embryonic fibroblast cells was cultured in 6-well plates on gelatin (0.1%, Sigma-Aldrich G7041-100G) at a seeding density of 1.6 x 104 cells/cm2 in base medium consisting of DMEM (1x ...
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bioRxiv - Developmental Biology 2023Quote: ... a feeder layer of inactive murine embryonic fibroblast cells was cultured in 6-well plates coated with 0.1% gelatin (Sigma-Aldrich G7041-100G) at a density of 1.6 x 104 cells/cm2 in a base medium containing DMEM (1x ...
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bioRxiv - Molecular Biology 2023Quote: ... 6- to 12-week-old mice were orally administrated with 180mg/kg of Tamoxifen (TAM) (Sigma-Aldrich, St. Louis, MO, T5648-5G) for 5 days ...
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bioRxiv - Cancer Biology 2024Quote: ... BxPC-3 cells were transduced with retroviral particles in a 6-well plate using polybrene transfection reagent (EMD MIllipore, TR-1003-G) and incubated for 48 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: Uninduced and induced samples were treated with either 0.2 μM or 2 μM Latrunculin A in DMSO (Sigma, CAS 76343-93-6) up to 4 h at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... For some experiments the PBS was adjusted to a final concentration of 6 mg/ml of poly-L-glutamate (Sigma- Aldrich, P4761). Plasmids were stored at 4°C for short-term and -20°C for long-term storage ...
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bioRxiv - Neuroscience 2023Quote: ... then in the anti-dopamine receptor antibody diluted in the blocking solution for 5-6 days at 4°C (rabbit anti-dopamine D1A receptor, 1:100, Sigma-Aldrich AB1765P). Sections were washed in PBS and incubated for 2 h at RT in the secondary antibody (goat anti-rabbit Alexa Fluor 555) ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde and the slide was mounted using a medium containing 4′,6-diamidino-2-phenylindole-DAPI (Fluoroshield, Sigma-Aldrich, USA). A fluorescence microscopy setup (Leica DM 2000 LED fitted with a Leica DFC450c camera ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were incubated at 37 °C and 5% CO2 for 24 hrs and selected with growth medium containing 6 μg/mL puromycin (Sigma-Aldrich, P8833) and 750 μg/mL neomycin (Thermo ...
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bioRxiv - Immunology 2023Quote: ... Plates were then blocked for 6 h with PBST (PBS with 0.01% Tween-20) containing 2% BSA (Sigma-Aldrich; cat. no. 10735094001) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... double-stained cells were also stained with 10 μM Laurdan (6-dodecanoyl-N,N-dimethyl-2-naphthylamine, Sigma Aldrich, Zwijndrecht, the Netherland). Laurdan was excited at 395 nm and 470 nm emission wavelength was used for rigid membrane and 508 nm for fluid membrane environment ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542) at RT for 2 hours ...
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bioRxiv - Systems Biology 2023Quote: ... and resuspended in 40 µl kinase buffer containing 300 µM ATP with 6 µCi γ-32P-ATP (Hartmann analytics) and 10 U bovine PKA (Sigma, 539576). For no PKA control ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were selected with 5 µg/ml blasticidin S and surviving colonies were isolated using 6 mm Pyrex® cloning cylinder (Sigma-Aldrich). Gene knockout was verified by western blotting of whole cell lysates with appropriate antibodies.
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bioRxiv - Cell Biology 2024Quote: ... Samples were permeabilised overnight at 4°C and then transferred to blocking solution (10% DMSO, 6% normal donkey serum (NDS; Sigma-Aldrich, D9663), 0.2% Triton X-100 in DPBS ...
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bioRxiv - Genomics 2024Quote: ... neural crest cells were passaged onto fibronectin-coated 6-well plates (about 1 million cells per well) using Accutase (Sigma-Aldrich A6964) and fed with neural crest maintenance medium (1:1 ratio of DMEM-F12 and neurobasal ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293TACE2 and A549ACE2 cells were generated by a transduction with a lentivirus expressing human ACE2 (kind gift from Benjamin Murrel, Karolinska Institutet) in the presence of 6 μg/ml Polybrene (Sigma-Aldrich, H9268). Clones were isolated and selected for homogeneous ACE2 expression as determined by HTM ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies at RT for 2 hours with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542). Afterward ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for two days at 4°C with secondary antibody along with 0.01 mg/ml 4′-6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich, Cat#: D9542) to label cell nuclei fluorescently and either 0.2 µl/ml phalloidin-iFluor 594 (Abcam ...
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bioRxiv - Immunology 2024Quote: ... IL-6 and IL-17A were assessed using the High Sensitivity T-cell Discovery Array 3-Plex (Millipore, St. Charles, MO, USA) at Eve Technologies using the Bio-Plex™ 200 system (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... Pre-seeded A549 cells were subsequently transduced with the lentivirus in the presence of 6 μg/ml of polybrene (Sigma-Aldrich, TR1003). After 24 hours ...
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bioRxiv - Neuroscience 2024Quote: Zebrafish larvae at 5 and 6 dpf were embedded in 1% low-melting temperature agarose diluted into MilliQ water (Sigma-Aldrich, USA). The fish were covered with artificial cerebro- spinal fluid (ACSF ...
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bioRxiv - Developmental Biology 2021Quote: PAs from homozygous a2KO (n = 5) and WT (n = 6) embryos were manually dissected in ice-cold DEPC-treated phosphate-buffered saline (DPBS, Sigma catalog number D8537). Individual PAs were pooled into four samples (WT PA1 ...
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bioRxiv - Neuroscience 2021Quote: ... 2007) was performed following a protocol described earlier with nuclei counterstained by 4’ 6-diamidino-2-phenylindole (DAPI, Sigma, D9542 10MG, 1:1000). To further assess the colocalization of different channels ...