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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Mouse Mitogen Activated Protein Kinase Kinase Kinase MLT MLTK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 20 µM DTT and phosSTOP in the presence of recombinant cyclin A2/cyclin-dependent kinase (CDK)1 (Sigma-Aldrich) and 10 mM ATP incubated for 2 h at 30 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 2004) and incubated overnight in 100 ml reaction buffer (kinase buffer + 0.2 mg/ml BSA (BSA Fraction V, Sigma) + 50 µg/ml kanamycin) ...
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bioRxiv - Biochemistry 2023Quote: ... Kinase-treated cells were then labeled with a 1:1000 dilution of biotinylated 4G10 Platinum anti-phosphotyrosine antibody (Millipore) for an hour on ice and washed with PBS containing 0.1% BSA and 2 mM EDTA (isolation buffer) ...
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bioRxiv - Molecular Biology 2023Quote: ... v-abl transformed Bcl2/pMX-INV infected pro-B cells (1 million per ml) were treated with 0.5 uM of the v-abl kinase inhibitor ABLki (PD180970, Sigma) and assayed for rearrangement by FACS analysis of GFP expression at 0 ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM magnesium chloride pH 7.4) and incubated overnight in 100 ml reaction buffer (kinase buffer + 0.2 mg/ml BSA (BSA Fraction V, Sigma) + 50 μg/ml kanamycin) ...
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bioRxiv - Molecular Biology 2021Quote: ... and cultured in F-media (62) containing 10 μM Y-27632 Rho-kinase inhibitor (63) in the presence of mitomycin C (M4287; Sigma) treated 3T3 J2 fibroblasts ...
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bioRxiv - Biochemistry 2020Quote: ... phosphorylation of peptides by the active Pro-directed kinase GST-CDK2/Cyclin A2 (100 ng purified from Sf9 cells, Sigma) was also confirmed in the presence or absence of the ATP competitive inhibitor ...
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bioRxiv - Biophysics 2021Quote: COS-7 cells transfected with WT tau alone or with fyn kinase were similarly stained with tau-5 (Sigma-Aldrich) or with tau-9g3 primary antibody (GeneTex Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... splenic B-cells from MD4 mice were pretreated or not with 5 μM of the Src kinase inhibitor PP2 (Millipore) (Cheng et al ...
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bioRxiv - Systems Biology 2021Quote: Cell fractions were thawed on ice and endogenous kinases were inhibited with 5′-[p-(fluorosulfonyl)benzoyl]adenosine (FSBA; Sigma-Aldrich) in DMSO at a final concentration of 1 mM FSBA and 10% DMSO in Tris-HCL ...
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bioRxiv - Plant Biology 2022Quote: AMPK3 and 4 activity was determined using a coupled enzyme assay with pyruvate kinase and lactate dehydrogenase (LDH, Sigma, P0294), measuring the production of NAD+ that results in absorption-decrease at 340 nm ...
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bioRxiv - Molecular Biology 2019Quote: ... For “+ATP” conditions 2 mM ATP and an ATP regeneration system (4.5 mM phosphoenolpyruvate, 20 ng/ml pyruvate kinase (Sigma-Aldrich)) were added to the reaction ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were transfected with 0.5 µg/well of pLKO.1-puro Vector empty or with plasmids containing the target shRNA sequence for the indicated kinases (Mission Library, Sigma). 48h after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... Responsiveness to NGF was assayed by probing for the expression of tropomyosin receptor kinase A (Trk-A; 1:2000; #06-574, Millipore-Sigma) [48] ...
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bioRxiv - Immunology 2021Quote: ... ubiquitin-conjugating enzyme E2 N (UBE2N) inhibitor, Ubc13 inhibitor (Rhyasen et al., 2013) and IκB Kinase inhibitor (PS-1145 dihydrochloride) were purchased from Sigma-Aldrich. LN cells from C57BL6 mice were treated with 10μM of IRAK-Inh ...
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bioRxiv - Molecular Biology 2020Quote: ... Single-cell iAT2s were plated on Transwells at a density of 520,000 live cells/cm2 in 100ul of CK+DCI+Y (3uM CHIR99021, 10ng/ml KGF, 50nM dexamethasone, 0.1mM cAMP, 0.1mM IBMX, 10uM Rho-associated kinase inhibitor (Sigma Y-27632)) ...
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bioRxiv - Molecular Biology 2023Quote: ... ADP was produced by incubating radioactive ATP with cold ADP and nucleotide di-phosphate kinase (NDK, N2635-100UN, Sigma-Aldrich) for 30 min at 25°C ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... and 0.1% NP-40) supplemented with 5 mM ATP and an ATP regeneration system consisting of 50 µg/mL creatine kinase (Millapore-Sigma) and 5 mM creatine phosphate (Millipore-Sigma) ...
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bioRxiv - Biophysics 2023Quote: ... 2 ml of cell culture medium containing either 10 μM Rho-kinase (Rock) Inhibitor Y-27632 (Sigma-Aldrich, St. Louis) or identical amounts of PBS (control ...
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bioRxiv - Biochemistry 2022Quote: ... Phosphorylation of kinase-dead Pyk2 was assessed via anti-phosphotyrosine Western blotting using anti-phospho-PTK2B pTyr402 antibody (SAB4300173, Sigma).
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bioRxiv - Genetics 2024Quote: ... differentiation of iPSCs was induced through the Wnt modulation method with 7 μM glycogen synthase kinase 3 b (GSK3b) inhibitor CHIR99021 (Selleckchem) and 5 μM inhibitor of WNT production 2 (IWP2) (Sigma). Basal Media was composed of RPMI media supplemented with B27 (Thermo Fisher Scientific) ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... and ADP production was quantitatively coupled to the stoichiometric consumption of NADH via the activity of excess pyruvate kinase (PK, type-III from rabbit muscle, Sigma) and lactate dehydrogenase (LDH ...
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bioRxiv - Cancer Biology 2021Quote: ... For the inhibition of Thymidylate kinase (DTYMK) cells were incubated for 72 h after adding 2 μM (final conc.) of the YMU-1 compound (Sigma-Aldrich), solved in 15 % DMSO ...
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bioRxiv - Biophysics 2022Quote: ... and an ATP-regeneration system (4 mM ATP, 50 μg/mL creatine kinase [Sigma-Aldrich], 5 mM creatine phosphate [Sigma-Aldrich]). The endpoint fluorescence for complete FITC-casein degradation was determined by addition of porcine elastase (100 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Responsiveness to NGF was assayed by probing for the expression of tropomyosin receptor kinase A (Trk-A; 1:2000; #06-574, Millipore-Sigma) [48] ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Physiology 2019Quote: ... All solutions were thawed from stocks at the day of experiments and freshly supplemented with creatine kinase (CK, Sigma/Roche, Germany) to ~300 U/ml or ~3 U/well and sodium azide (0.1 M NaN3) ...
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bioRxiv - Developmental Biology 2022Quote: ... Inhibition of EGFR signaling was performed by injecting 80 μg/g of the kinase inhibitor PD153035 (CAS: 183322-45-4; Sigma-Aldrich), dissolved in DMSO/1xPBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The kinase domain of human HASPIN (residue 452 – 798) was cloned into the expression vector pET-21a(+) (Novagen, Madison, WI, USA). The genomic DNA of HASPIN was provided from the Korea Human Gene Bank ...
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bioRxiv - Cancer Biology 2023Quote: Fresh EAC tumor samples were transferred into ice cold conditioned PBS (formula: 10uM rho-associated kinase (ROCK) inhibitor Y27632 (Sigma, #Y0503), 2% penicillin-streptomycin sulfate (Gibco ...
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bioRxiv - Biophysics 2023Quote: ... phosphoenol pyruvate (53 mM PEP, Beantown Chemical, 129745) and pyruvate kinase/lactate dehydrogenase enzymes (2.8% v/v PK/LDH, Sigma, P-0294). For experiments with PRC1 ...
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bioRxiv - Biochemistry 2023Quote: ... The preactivated kinase mixture was then desalted and concentrated using an Amicon™ Ultra-15 30 kDa MWCO spin filter (Millipore) to remove the residual ATP.
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bioRxiv - Immunology 2023Quote: Cells obtained by crawl-out were incubated for 24h at 37°C with the phosphatidylinositol-3-kinase inhibitor wortmannin or the carnitine palmitoyltransferase-1 inhibitor etomoxir (both from Sigma-Aldrich), at 10 and 200μM respectively.
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bioRxiv - Molecular Biology 2023Quote: ... A stock ATPase reaction mix (20X) contained 20 μL of a mixture of pyruvate kinase and lactic dehydrogenase from rabbit muscle (P0294, Sigma Aldrich), 10 μL of 200 mM NADH (CAS# 606688) ...
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bioRxiv - Biochemistry 2023Quote: ... Full-length BTK and BTK linker kinase domain (WT and mutants) were produced by co-expressing with YopH in BL21(DE3) (Millipore Sigma) or BL21-Gold(DE3 ...
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bioRxiv - Biochemistry 2023Quote: GS activities were measured using the pyruvate kinase/ lactate dehydrogenase (PK/LDH) coupled enzymes from rabbit muscle ordered from Sigma-Aldrich (containing 600-1,000 units/ml pyruvate kinase 900-1,400 units/ml lactate dehydrogenase) ...
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bioRxiv - Physiology 2023Quote: ... Analysis of the muscle damage marker creatine kinase (CK) was quantified using spectrophotometry using a coupled enzyme-linked reaction assay (Sigma-Aldrich). Samples were measured in duplicate ...
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bioRxiv - Molecular Biology 2023Quote: ... the c-Jun N-terminal kinase inhibitor SP600125 (Cat. S5567) and the c-Myc inhibitor 10058-F4 (Cat. F3680) were from Millipore Sigma.
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bioRxiv - Physiology 2024Quote: ... to activate transient receptor potential kinase ankyrin 1 [TRPA1] channels; 10 µM for 5 min, from a stock concentration of 1 M in DMSO, Sigma-Aldrich); HC-030031 (to block TRPA1 channels ...
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bioRxiv - Plant Biology 2024Quote: ... a similar reaction was performed with both enzymes combined with 10 units phosphoglycerate kinase and 10 units glyceraldehyde-3-phosphate dehydrogenase (both Sigma-Aldrich), alongside 9 mM phospho-enol-pyruvate ...
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Inhibiting the prostaglandin transporter PGT induces non-canonical thermogenesis at thermoneutralitybioRxiv - Physiology 2019Quote: ... Serum was collected and serum creatine kinase activity was measured according to manufacturer’s instructions (Cat# MAK116, Sigma-Aldrich, St. Louis, MO, USA)
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... an ATP regeneration system (2 mM ATP, 30 mM phosphoenol pyruvate and 30 U of pyruvate kinase) was used (Sigma-Aldrich, USA). For the inverse reaction ...
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Single-cell iAT2s were plated on Transwells at a density of 520,000 cells/cm2 in 100 μl of CK+DCI medium containing 10 μM of Rho-associated kinase inhibitor (“Y”; Sigma Y-27632). 600 μl of this medium was added to the basolateral compartment ...
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bioRxiv - Systems Biology 2023Quote: ... and resuspended in 40 µl kinase buffer containing 300 µM ATP with 6 µCi γ-32P-ATP (Hartmann analytics) and 10 U bovine PKA (Sigma, 539576). For no PKA control ...
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bioRxiv - Biophysics 2023Quote: Inhibition of Rho-kinase on NK92 cells (Fig. 4) is performed with Y-27632 Rock-inhibitor (Sigma-Aldrich, cat. no. 129830-38-2). Y-27632 Rock-inhibitor is dissolved in DMSO (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid that contains the kinase domain of human HASPIN was transformed into Escherichia coli strain Rosetta™ 2(DE3) pLysS (Novagen, Madison, WI, USA). The transformed cells were grown at 37□ until OD600 reached 0.8 ...
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bioRxiv - Physiology 2021Quote: ... mouse FGF21 ELISA kit (Sigma-Aldrich, EZRMFGF21-26K).
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bioRxiv - Neuroscience 2023Quote: ... anti-mouse IL-6 ELISA kit (Sigma, RAB0309), anti-mouse G-CSF ELISA kit (MCS00 ...