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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Mouse Mitogen Activated Protein Kinase Kinase Kinase MLT MLTK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Y15 [focal adhesion kinase (FAK) inhibitor] (1,2,4,5-benzenetetramine tetrahydrochloride) (Sigma-Aldrich Cat# 406-66-5); SEW 2871.
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bioRxiv - Plant Biology 2024Quote: ... 4 mM phosphoenolpyruvate (PEP)) as well as 1.2 U pyruvate kinase (P1506; Sigma-Aldrich/Merck) were added to convert ADP and PEP to ATP and pyruvate ...
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bioRxiv - Cell Biology 2024Quote: ... the myotubes were treated with tyrosine kinase inhibitors (TKIs) – Sorafenib (SML2653-5MG, Sigma-Aldrich, Germany), imatinib mesylate (S1026 ...
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bioRxiv - Cell Biology 2024Quote: ... then the reactions were incubated in kinase buffer with 20 μM ATPγS (Sigma-Aldrich; A1388) at 30°C for 45 min ...
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bioRxiv - Biochemistry 2020Quote: ... RIPK1 kinase was performed on KISSf screening facility (IBISA, Biogenouest, Station Biologique, Roscoff, France) with myelin basic protein (MBP, Sigma, #M1891) as substrate and in the presence of 15µM ATP.
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bioRxiv - Immunology 2023Quote: ... were formed by dissociating and maintaining PSCs in ESC media supplemented with 10 μM Rho-associated protein kinase (ROCK) inhibitor (Sigma, Y0503) while being kept on an orbital shaker at 100 rpm for 6 days to allow for spontaneous formation of EBs with hematopoietic potential ...
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bioRxiv - Molecular Biology 2019Quote: BJ1hTERT fibroblasts were treated with either a kinase inhibitor that prevents DNA-PKcs autophosphorylation (NU7026, Sigma) or MRE11 endonuclease activity inhibitor (PFM01 ...
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bioRxiv - Cell Biology 2021Quote: ... a single dose (5 μM) of the Casein Kinase 1 δ/ε inhibitor PF670462 (Sigma #SML0795) was added to the medium on days 3 ...
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bioRxiv - Molecular Biology 2020Quote: ... The kinase activity of Cdk1-as1 was inhibited by 1-NM-PP1 (EMD Millipore, CAS# 529581) with the concentration of 25 μM.
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bioRxiv - Cell Biology 2023Quote: CRAF kinase activity was measured as described previously (17) and according to the manufacture’s protocol (Millipore). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... Pyruvate kinase of rabbit muscle and Malate Dehydrogenase of pig heart muscle were purchased from Sigma. Protein concentrations were determined with via Bradford assay (Bio-Rad Protein Assay Dye Reagent).
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bioRxiv - Microbiology 2024Quote: ... and incubated overnight in 100ml reaction buffer (kinase buffer + 0.2mg/ml BSA (BSA Fraction V, Sigma) + 50□g/ml kanamycin) ...
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bioRxiv - Developmental Biology 2024Quote: ... the c-RAF kinase construct was induced by adding 250 nM 4OHT (4-Hydroxytamoxifen, Sigma, H7904) to the media.
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bioRxiv - Microbiology 2020Quote: ... Resulting primary cells were first cultured in the selective media to proliferate in the presence of the Rho-associated protein kinase (ROCK) inhibitor (Y-27632, 10 µg/ml, Sigma-Aldrich, Poland) (95) ...
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bioRxiv - Plant Biology 2023Quote: ... 100 ng purified recombinant CTR1-KD or 1 µg MPK4 was mixed with 2 µg Myelin Basic Protein (MBP, a common kinase substrate, Sigma Cat. M1891) and 1 mM ATPγS in kinase reaction buffer (10 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... Serum samples were collected from surviving mice and 3 samples from each group were used for estimation of Creatine Kinase activity Assay Kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... kinase assays were performed by incubating the immunoprecipitated proteins and beads in 30 μl kinase buffer supplemented with 50 μM ATP and 3 μg histone H1 (EMD Millipore, 14-155) for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... 1.8 μg/mL VEGF Receptor Tyrosine Kinase Inhibitor II (VEGFR-TKI II; Calbiochem/EMD Millipore #676481-5MG) dissolved in DMSO was used to disrupt blood vessels57 and 100 μM γ-secretase inhibitor IX (DAPT ...
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bioRxiv - Developmental Biology 2021Quote: ... VEGF Receptor Tyrosine Kinase Inhibitor II (VEGFR-TKI II; Calbiochem/EMD Millipore #676481-5MG, 1.8 μg/mL) or equivalent amount of DMSO vehicle was added to media.
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bioRxiv - Biochemistry 2020Quote: ... was added to reaction mixtures containing Pyruvate Kinase and Lactic Dehydrogenase enzymes (Sigma-Aldrich, Gillingham, United Kingdom) with 0.010-0.016 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 50 mM.min-1 pyruvate kinase and 50 mM.min-1 lactate dehydrogenase (as assayed by the manufacturer, Sigma). Ligases were diluted prior to assay as required in 50 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2019Quote: ... ATPγS and T4 polynucleotide kinase (3’-phosphatase free) were obtained from Sigma-Aldrich (St. Louis, Missouri, USA). Biotin maleimide was purchased from Vector Laboratories (Burlingame ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mM phosphoenolpyruvate (PEP) (Molecula, 16921512) and 16 units/ml lactic dehydrogenase/pyruvate kinase enzymes (Sigma, P0294). 30 mL reactions were prepared in 384 well plates (Greiner ...
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bioRxiv - Neuroscience 2022Quote: ... NBD supplemented with 10 μM of Rho-Kinase Inhibitor Y27632 (10 mM in PBS, 688000, Sigma Aldrich), as this was shown to improve survival of thawed iPSC-derived cells (Chen et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and commercial pyruvate kinase (PK) and lactate dehydrogenase (LDH) from Saccharomyces cerevisae (Sigma-Aldrich, Saint-Louis, USA) (Figure S2) ...
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bioRxiv - Developmental Biology 2022Quote: ... medium with an additional 10 μM of the Rho kinase inhibitor Y-27632 (Sigma-Aldrich, Y0503-1MG) in ENRWN was used for recovery ...
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bioRxiv - Plant Biology 2024Quote: ... both enzymes were combined with 10 units pyruvate kinase and 10 units lactate dehydrogenase (both Sigma-Aldrich) at 25°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The purified BGLF4 was then used for kinase inhibition assays using the C-terminal fragment peptide of retinoblastoma protein (RB) as substrate (Millipore Sigma cta# 12-439). The remaining assay parameters were the same as those applied for PknB except for the following changes ...
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bioRxiv - Cell Biology 2020Quote: ... The PI4 kinase inhibitors GSK-A1 (SYNkinase, Parkville, Australia) and phenylarsine oxide (PAO, Sigma Aldrich, St Louis, US) were dissolved in 1 mM (GSK-A1 ...
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bioRxiv - Plant Biology 2019Quote: ... supernatant was diluted 1 in 10 in pyruvate kinase reaction buffer (85 mM Tris-HCl pH 7.4, 1.7 mM MgSO43.5 mM phosphoenolpyruvate, 23U pyruvate kinase (Sigma-Aldrich)) for 30 mins at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... and 15 mM phosphoenolpyruvate in presence of a 4% v/v pyruvate kinase/lactate dehydrogenase enzyme mix (Sigma). Reactions were initiated by the addition of ATP to a final concentration of 25 μM - 6.4 mM ...
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bioRxiv - Microbiology 2021Quote: ... for ATP quantification or BIII/PEP+PK (BIII/PEP with 2 U/µL pyruvate kinase (Sigma-Aldrich, P1506) for ATP + ADP quantification ...
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bioRxiv - Cancer Biology 2022Quote: ... a selective inhibitor of TGFβ Type 1 receptor kinases (TGFR1i) was purchased from Sigma-Aldrich (St. Louis, MO) and used at a concentration of 10 µM in all experiments.
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bioRxiv - Cancer Biology 2022Quote: ... The washed beads were resuspended in 25 μl of kinase assay buffer with 100 μM ATP (A7699, Sigma) supplemented with phosphatase inhibitors and incubated for 20 min in an Eppendorf Thermomixer at 30°C at maximal mixing velocity ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were maintained overnight in mTeSR1 medium containing the Rho-associated kinase (ROCK) inhibitor Y-27632 (Sigma-Aldrich) until cells reached 60-70 % of confluency ...
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bioRxiv - Cell Biology 2022Quote: The enzymatic activity of pyruvate kinase was measured using EC 2.7.1.40 from Sigma (Bergmeyer, H.U. et al., n.d.) as described previously (Lewandowski et al. ...
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bioRxiv - Biophysics 2020Quote: ... The coupled enzymatic reaction contained ~2 units of pyruvate kinase/lactic dehydrogenase (rabbit muscle isolate mixture, Sigma Aldrich) 50 mM KPi ...
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bioRxiv - Microbiology 2022Quote: ... 1.1 U.ml−1 lactate dehydrogenase and 0.8 U.ml−1 pyruvate kinase (rabbit muscle, P0294 from Sigma-Aldrich®) and 0.5 mg.ml−1 MtAPSK (all final concentrations ...
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bioRxiv - Neuroscience 2022Quote: ... we delivered the Iκ-kinase inhibitor [5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide (TPCA-1; Sigma-Aldrich CAS 507475-17-4 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were maintained overnight in mTeSR1 medium containing the Rho-associated kinase (ROCK) inhibitor Y-27632 (Sigma Aldrich) until cells reached 60–70% of confluency ...
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bioRxiv - Physiology 2023Quote: ... USA) fitted onto a 96-well plate and calibrated with a standard curve of purified creatine kinase (Sigma, Oakville ...
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bioRxiv - Molecular Biology 2023Quote: ... and the second parotid salivary gland was analyzed using the pyruvate kinase activity assay kit (cat. No. MAK072, Sigma-Aldrich, St. Louis, MO). For both activity assays ...
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bioRxiv - Molecular Biology 2020Quote: Hep-2 target cells were incubated with increasing concentrations of anti-EGFR antibodies (0 to 500 ng/mL) in the presence of Jurkat CD16 cells and the protein kinase C activator phorbol- myristate acetate (P8139, Sigma-Aldrich, St Louis, MO, USA). After 16 h of incubation at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Singlecell iAT2s were plated on Transwells at a density of 520,000 live cells/cm2 in 100μl of CK+DCI with 10μM Rho-associated kinase inhibitor (“Y”; Sigma Y-27632). 600μl of CK+DCI+Y was added to the basolateral compartment ...
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bioRxiv - Microbiology 2020Quote: ... with 0.5mM nicotinamide adenine dinucleotide (NADH) and 2mM phosphoenolpyruvate and 3µl of a pyruvate kinase/lactate dehydrogenase mix (PYK/LDH; Sigma). PomXNPEP and PomXNPEP_K13AR15A peptides (MKKAFEQNVSRAKPRLRLGALT and MKKAFEQNVSRAAPALRLGALT ...
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bioRxiv - Bioengineering 2022Quote: ... and a medium with an extracellular signal-regulated kinase inhibitor (ERKi, MilliPore Sigma #1049738-54-6, Burlington, MA, USA), adapted from Eigler et al.2 More details on the media used are provided in Supplementary Table 1.
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bioRxiv - Developmental Biology 2020Quote: ... per manufacturer’s instructions and seeded onto the micropattern at 132,000 – 263,000 cells/cm2 in mTeSR with 10 μM Rho-associated kinase inhibitor (ROCKi Y-27632, Millipore Sigma). After 6h ...
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bioRxiv - Cell Biology 2020Quote: ... 2005) in which the final concentrations of coupling reaction components were adjusted as follows: Pyruvate kinase (Sigma Aldrich, P9136) – 400 units/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Sorted cells were cultured in Matrigel® drops with ENR_CV-medium plus the Rho Kinase inhibitor Y-27632 (Sigma) to obtain a new in vitro line of intestinal organoids named Lgr5-EGFP/RCL-tdT ...
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bioRxiv - Biochemistry 2022Quote: ... along with an ATP regeneration system consisting of 187.5 U mL−1 pyruvate kinase and 50 mM phosphoenolpyruvate (Sigma). For gel degradation assays ...