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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Mouse Mitogen Activated Protein Kinase Kinase Kinase MLT MLTK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 0.05 mg/mL pyruvate kinase from rabbit muscle (Sigma-Aldrich, Schnelldorf, Germany), 0.5 mM NADH) ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 μM glycogen synthase kinase (GSK) inhibitor (Calbiochem, D00163483 or Sigma, CHIR99021), 10 μM MEK inhibitor (Tocris ...
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bioRxiv - Biochemistry 2024Quote: ... 0.04 U/μl pyruvate kinase/0.05 U/μl lactic dehydrogenase (Sigma-Aldrich) and 5 mM ATP ...
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bioRxiv - Cancer Biology 2024Quote: CAMK2A D135N kinase domain was recombinantly expressed in BL21(DE3) cells (Millipore) as previously described32 ...
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bioRxiv - Molecular Biology 2023Quote: The enzymatic activity of the TaSnRK1α was assessed using the Universal Fluorometric Kinase Assay Kit (Sigma). For kinase assay ...
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bioRxiv - Cell Biology 2022Quote: Purified GFP-Cdt1 protein (1.6 mg ml-1) was treated with CDK1-CyclinB Kinase (EMD Millipore, # 14-450) supplemented with 10 mM ATP in kinase buffer (20 mM Tris pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... in CK+DCI with 10 μM Rho-associated kinase inhibitor (Sigma Y-27632). At 48h after plating ...
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bioRxiv - Cancer Biology 2020Quote: ... and 5 mmol/L Rho kinase inhibitor Y27632 (Sigma-Aldrich, St. Louis, MO). After 1 month of passages ...
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bioRxiv - Bioengineering 2021Quote: ... with the addition of 10 μM Rho kinase inhibitor (ROCKi, Y-27632; Sigma). After 24h ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2 mM NADH and 25 units/mL lactate dehydrogenase/pyruvate kinase (Sigma-Aldrich) to monitor the NADH dependent fluorescence signal in non-binding ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 mM sodium dichloroacetate (an inhibitor of pyruvate dehydrogenase kinase, Sigma-Aldrich) was used ...
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bioRxiv - Cell Biology 2021Quote: ... the myosin light chain kinase (MLCK) inhibitor ML-7 (10 μM, EMD Millipore), or the Ca2+ inhibitor 2APB (10 μM ...
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bioRxiv - Biochemistry 2022Quote: ... 10 u/ml pyruvate kinase/lactate dehydrogenase (from rabbit muscle, Sigma Aldrich, #P0294) and 5 mM MgCl2 in SEC buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Excess pyruvate kinase (PK) / lactate dehydrogenase (LDH) enzymes from rabbit muscle (Sigma P0294), ADP ...
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bioRxiv - Biochemistry 2019Quote: ... 20 units of enzymes (lactate dehydrogenase (LDH) and pyruvate kinase (PK)) (Sigma-Aldrich), 4 mM ATP ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2 mM NADH and 25 units/mL lactate dehydrogenase/pyruvate kinase (Sigma-Aldrich) to monitor the NADH dependent fluorescence signal in non-binding ...
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bioRxiv - Biochemistry 2020Quote: ... 5.6 U/ml pyruvate kinase/ 7.8 U/ml lactate dehydrogenase (Sigma, cat: P0294), TFIIH (1.2 nM yeast variants or 6 nM human variants) ...
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bioRxiv - Biochemistry 2020Quote: ... and pyruvate kinase/lactic dehydrogenase enzyme mix (diluted final 1:45; Sigma, P0294). Where indicated 3 mM CaCl2 was present ...
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bioRxiv - Neuroscience 2021Quote: ... cells were preincubated with 150 nM K-252a (tyrosine kinase blocker, #K1639, Sigma), 1 μM ANA-12 (TrkB receptor antagonist ...
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bioRxiv - Immunology 2021Quote: ... and 10 μM Rho kinase (ROCK) inhibitor (Y-27632) dihydrochloride monohydrate (Sigma-Aldrich). Caecaloids were cultured at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... The first 4 days of culture the Rho kinase inhibitor Y-27632 (Sigma) was added to the culture ...
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bioRxiv - Immunology 2022Quote: The animals were injected with TGF-β RI Kinase Inhibitor SB431542 (Sigma-Aldrich) at 10 mg/kg in 100 μl DMSO intraperitoneally for continuous three days.
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bioRxiv - Cancer Biology 2022Quote: ... AKT1/2 kinase inhibitor was purchased from Sigma-Aldrich (St. Louis, MO, USA). IGF-1 was purchased from PeproTech Inc (Rocky Hill ...
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bioRxiv - Microbiology 2023Quote: Kinase inhibitor H-89 dihydrochloride was purchased from Sigma-Aldrich (Merck, Darmstadt, Germany). The H-89 analogue 97i was a gift from prof ...
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bioRxiv - Biophysics 2023Quote: ... and 3-7 units/mL of pyruvate kinase and lactate dehydrogenase (Sigma P0294). An NADH standard curve was measured in order to convert absorbance values to the concentration of ATP hydrolyzed ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 nM TGF-b RI Kinase Inhibitor IV (A83-01) (Millipore, #616454-2MG), 10 mM Nicotinamide (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... phospho-p70 S6 Kinase (Thr389) (Cat. No. MAB S82, Millipore Sigma, Burlington, MA) GAPDH (Cat ...
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bioRxiv - Neuroscience 2023Quote: ... in combination with anti-Ca2+/calmodulin-dependent protein kinase II α (CKII; monoclonal mouse IgG1, 05-532, clone 6G9, EMD Millipore Corporation, Temecula, USA), diluted 1:4000 ...
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bioRxiv - Developmental Biology 2019Quote: ... into single-cell suspension and re-suspended in E8 media containing 10 μM Rho-associated protein kinase inhibitor (Sigma). Approximately 100,000 cells were re-plated into Matrigel-coated 6-well plates ...
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bioRxiv - Biochemistry 2020Quote: Standard kinase reaction conditions: 70 mM HEPES-NaOH with pH= 7.5 (Sigma; Cat. # H3375), 3 mM MgCl2 (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... GW9662 and Akt I-II kinase inhibitor were purchased from Sigma-Aldrich (Madrid, Spain)
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bioRxiv - Cancer Biology 2020Quote: ... 30% WNT conditioned medium (home-made) and 10μm Rho-kinase inhibitor (Y27632, Sigma-Aldrich), for the first week ...
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bioRxiv - Cell Biology 2022Quote: ... then the reactions were incubated in kinase buffer with 20 μM ATPγS (Sigma-Aldrich) at 30°C for 45 min ...
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bioRxiv - Biochemistry 2019Quote: Casein phosphorylation reactions were performed with 300ng kinase and 25µM dephosphorylated alpha casein (Sigma) in PMP buffer plus 100μM cold ATP ...
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bioRxiv - Neuroscience 2019Quote: To block cell proliferation the cyclin-dependant kinase (CDK) inhibitors Olomoucine and Ryuvidine (Sigma) where applied ...
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bioRxiv - Neuroscience 2020Quote: ... Src-kinase inhibitor (2 µM SU 6656, S9692 Sigma-Aldrich, St. Louis, MO, USA) was added ...
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bioRxiv - Physiology 2022Quote: ... 2.4–4 units of pyruvate kinase/ 3.6–5.6 units lactate dehydrogenases enzymes (Sigma, P0294).
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bioRxiv - Bioengineering 2022Quote: ... The first 4 days of culture the Rho kinase inhibitor Y-27632 (Sigma-Aldrich) was added ...
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bioRxiv - Synthetic Biology 2023Quote: A two-enzyme cascade comprising Cytophaga PPK2 and rabbit creatine kinase (CK) (Sigma-Aldrich) was applied to sequentially convert the algal polyP into creatine phosphate via ATP ...
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Plant Biology 2020Quote: ... AtMPK4 and AtMPK6 was performed using anti-MAP kinase-antibody (1:20000 dilutions) as primary antibody and anti-mouse IgG (Sigma) protein (1:15000 dilutions ...
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bioRxiv - Biophysics 2022Quote: ... and an ATP-regeneration system (4 mM ATP, 50 μg/mL creatine kinase [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2019Quote: ... The 63 nucleotides long HSV thymidine kinase minimal promoter (TK mini) synthesized by SIGMA-ALDRICH, was added upstream of luciferase gene ...
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bioRxiv - Cell Biology 2020Quote: ... ATP regeneration system (10 mM creatine, 0.45 mg ml−1 phospho-creatine kinase (Sigma-Aldrich). During initial stages of this work ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were treated with the dual non ATP-competitive sphingosine kinase inhibitor SKI-II (SIGMA), S1P (Avanti Polar ...
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bioRxiv - Cell Biology 2021Quote: ... The rest of the kinase inhibitors were purchased as indicated: Staurosporine (Sigma Aldrich, catalog # 569397), LJI308 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: The enzymatic assay of pyruvate kinase (EC 2.7.1.40) is from Sigma (Bergmeyer, H.U. et al.), with modifications for a 96-well plate format ...
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bioRxiv - Bioengineering 2021Quote: ... the culture media was often supplemented with the Rho kinase inhibitor Y-27632 (Sigma-Aldrich) at a 10 μM concentration to encourage cell survival and establish clonal lines ...
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bioRxiv - Cell Biology 2022Quote: ... LTBP3 KD cells were treated with 2 μM kinase inhibitor SB431542 (Millipore Sigma; S4317-5MG) or DMSO as a vehicle control for SB431542 in basal media for 4 h followed by incubation in differentiation media supplemented with 2 μM inhibitor for 3 to 5 days ...
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bioRxiv - Biophysics 2023Quote: ... 4.5 μL pyruvate kinase/lactic dehydrogenase enzyme mixture from rabbit muscle (Sigma-P0294-5 mL) with 600 – 1000 units PK/mL and 900 – 1400 units LDH/mL ...