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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for 4 ArmPEG MA 10K since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 4-hydroxytamoxifen (4-OHT) (Sigma) was prepared by dissolution in ethanol (10% ...
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bioRxiv - Developmental Biology 2021Quote: 4-hydroxytamoxifen (4-OHT, Sigma) was dissolved in 100% ethanol to prepare a stock concentration of 10 mM ...
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bioRxiv - Genomics 2021Quote: ... An initial preamplification is conducted using extracted DNA with KOD Hot Start DNA Polymerase (71842-4, Millipore Sigma, Burlington, MA, USA) according to manufacturer’s instructions using all 5µL of extracted DNA in a total reaction volume of 20µL ...
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bioRxiv - Neuroscience 2021Quote: ... Cell lysates were separated using electrophoresis on 4-20% SDS-polyacrylamide gels and transferred to PVDF membrane (Millipore, Billerica, MA, USA). For western blot ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted fractions were concentrated (Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-30 membrane, EMD Millipore, Merck, Burlington MA, USA) and purified by size exclusion chromatography using a Superdex 200 Increase 10/300 (GE LS ...
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bioRxiv - Molecular Biology 2022Quote: ... Tissue lysates were separated by SDS-PAGE gels (4%-12%) and transferred to polyvinylidene difluoride fluorescence membranes (Millipore, Billerica, MA, USA). Non-fat dry milk (NFDM-10% ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... then blocked with 4% NHS/TBST-0.2% and incubated overnight at 4°C in primary antibody solution (1:2500 anti-tyrosine hydroxylase, clone LNC1, Millipore, Billerica, MA) in 2% NHS/TBST-0.2% ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... 100 mM NaCl pH 7.3 and concentrated to 22 mg/mL using Amicon Ultra-4 centrifugal filters (Millipore, Billerica MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated for 48 hr at 4°C with mouse anti-TH antibody (1:2,000; Millipore, Burlington, MA) in 0.1 M PBS containing 2% normal donkey serum and 0.1% Triton X-100 ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were concentrated by centrifugation in 4 mL of Amicon filter concentrators with a 10 kDa cutoff (Millipore Sigma; Burlington, MA). TCEP (1 mM ...
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bioRxiv - Microbiology 2023Quote: ... The infection was incubated for 2-3 hours at 37°C and then the viral inoculum was removed and replaced with a pH 7.2 methyl cellulose overlay medium (1% methyl cellulose [Sigma-Aldrich, MA]/5% FBS [Gibco, MA]/1X penicillin-streptomycin [Gibco, MA]/44 mM sodium bicarbonate [Sigma-Aldrich, MA]/DMEM #12100-046 [Gibco, MA]). After overnight incubation at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: 4-hydroxytamoxifen (4-OHT, Sigma, #7904) was dissolved in 100% ethanol (1 mM stock solution) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-hydroxytamoxifen (4-HT [Sigma, H7904]) was dissolved in methanol to a concentration of 5 mg/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... or 4-Hydroxytamoxifen (4-OH, Sigma) was dissolved to a final concentration of 1mg/ml or 3mg/ml in 90% corn oil (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 1μM 4-hydroxytamoxifen (4-OHT; Sigma) was added to select wells 5 days prior to imaging to allow for adequate Cre-mediated recombination and expression of MCT4 shRNA ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... 4 × 10−4 M monothioglycerol (Sigma), 50 µg/mL ascorbic acid (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: 4-PBA (4-phenylbutyric acid, Sigma) and 5-PVA (5-phenylvaleric acid ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-hydroxytamoxifen (4-OHT, Sigma, H7904) was dissolved in 100% DMSO at a concentration of 20mg/ml and 25 ul of the 4-OHT solution was injected via intramuscular injection into the left mouse gastrocnemius muscle ...
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bioRxiv - Developmental Biology 2021Quote: 4-hydroxytamoxifen (4-OHT; Sigma H7904) stock was prepared by dissolving the powder in ethanol ...
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bioRxiv - Cancer Biology 2022Quote: 4-hydroxytamoxifen (4-OHT, Sigma H6278) stock was prepared by dissolving the powder in ethanol to reach 5 or 10 mM concentration and thoroughly vortexed and aliquoted into 100 μl aliquots ...
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bioRxiv - Neuroscience 2022Quote: ... 4 aminopyridine (4-AP, 100µM; Sigma). The currents recorded at the same imposed potential were averaged for each cell to perform the quantification ...
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bioRxiv - Neuroscience 2022Quote: ... 4-aminopyridine (4-AP, Sigma, 275875), and gaboxadol (Sigma ...
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bioRxiv - Immunology 2022Quote: ... 4-hydroxytamoxifen (4-OHT from Sigma) was administered i.p ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... 4-hydroxytamoxifen (4-OHT; Sigma Aldrich) was diluted in ethanol following the manufacturer’s recommendation ...
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bioRxiv - Developmental Biology 2024Quote: 4-hydroxytamoxifen (4-HT, H7904; Sigma) was dissolved in ethanol at a final stock concentration of 5 mM and kept in single-use aliquots in the dark at –80°C ...
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bioRxiv - Cell Biology 2024Quote: 4-hydroxytamoxifen (4-OHT, Sigma H6278) was dissolved at 20 mg/mL in ethanol by shaking at 37°C for 15 minutes and was then aliquoted and stored at -20°C for up to several weeks ...
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bioRxiv - Developmental Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT; Sigma-Aldrich) was administrated to 12 weeks old male mice at a dose of 1.0mg/40g body weight via intraperitoneal injection for five consecutive days ...
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bioRxiv - Neuroscience 2023Quote: 4-hydroxytamoxifen (4-OHT; Sigma, H7904) was dissolved in corn oil/ethanol (90% corn oil ...
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bioRxiv - Neuroscience 2023Quote: ... 4-Hydroxytamoxifen (4-OHT; Sigma #H6278) was dissolved in corn oil according to a modification of published methods [24] ...
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bioRxiv - Neuroscience 2023Quote: 4-hydroxytamoxifen (4-HT, Sigma-Aldrich) was dissolved in 100% ethanol (10 mg/ml) ...
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bioRxiv - Neuroscience 2024Quote: 4-hydroxytamoxifen (4-OHT; Sigma H6278) at 20 mg/ml in ethanol was added to a 1:4 mixture of castor oil:sunflower seed oil (Sigma 259853 and Sigma S5007 ...
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bioRxiv - Neuroscience 2024Quote: 4-hydroxytamoxifen (4-OHT, Sigma H6278) was dissolved in 55°C DMSO at a concentration of 4 mg/mL and stored in 25 μL aliquots in -20°C ...
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bioRxiv - Immunology 2024Quote: ... 4-hydroxytamoxifen (4-OHT) (Sigma-Aldrich) was spiked into cultures at day 3 of differentiation to a final concentration of 500 nM.
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bioRxiv - Developmental Biology 2024Quote: 4-Methylumbelliferone (4-MU, Sigma Aldrich) was dissolved in DMSO to establish a 150 mM stock then stored at -20°C ...
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bioRxiv - Neuroscience 2023Quote: ... Near adjacent compartments of tissue were singly immunoreacted for anti-Calbindin D28k (CaBP,a marker of the A10 and A8 DA subpopulation 1:10K, Sigma #C9848, mouse,Haber and Fudge 1997), anti-CRF (1:1K ...
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bioRxiv - Genomics 2020Quote: Human melanoma cells were seeded in 12 well plates with increasing volumes of lentiviral supernatant in a final volume of 2 mL with 4 μg/mL polybrene (Millipore, Burlington, MA). Plates were centrifuged for 2 hours at 1,000x g at 30°C and cells were incubated for 16 hours at 37°C and 5% CO2 in a humidified incubator ...
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bioRxiv - Genomics 2020Quote: Human melanoma cells were seeded in 12 well plates with increasing volumes of lentiviral supernatant in a final volume of 2 mL with 4 μg/mL polybrene (Millipore, Burlington, MA). Plates were centrifuged for 2 hours at 1,000x g at 30°C and cells were incubated for 16 hours at 37°C and 5% CO2 in a humidified incubator ...
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bioRxiv - Neuroscience 2021Quote: ... for 12 h at 4°C after blocking for 30 min at room temperature with 5% donkey serum (S30-100ml, Millipore, MA, USA). Subsequently ...
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bioRxiv - Neuroscience 2021Quote: ... the cells and supernatants were harvested and purified using chloroform and were condensed using Amicon Ultra-4 Centrifugal Filter Devices (100K MWCO; Merck Millipore, Billerica, MA).
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bioRxiv - Microbiology 2020Quote: ... The elution buffer with rgp120 was exchanged for phosphate-buffered saline (PBS) using Amicon Ultra-4 Centrifugal Filter Unit (Millipore, Billerica, MA). The concentration of rgp120 protein was calculated by dividing optical density at 280 nm (NanoDrop 2000/2000c ...
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bioRxiv - Immunology 2022Quote: ... and was further purified by centrifugation at 10,000 xg for 5 minutes at 4°C through an Amicon Ultra 0.5 ml 100 kDa cutoff spin filter (Millipore, Billerica, MA, USA). The NEU3 was analyzed for size and purity by PAGE on ...
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bioRxiv - Immunology 2022Quote: ... the retrovirus supernatant was concentrated by centrifugation at 5000g at 4 °C for 2 h with Amicon Ultra-15 Centrifugal Filter Units (Millipore, Billerica, MA). A human T cell line (Jurkat ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 48 hr in blocking buffer containing a rabbit anti-c-Fos antibody (1:3000, ABE457, Millipore, Bellerica, MA). After washes in TNT (0.1 M Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... elution fractions were combined and concentrated to 0.5 – 1 mg/mL using Amicon Ultra-4 30 kDa MWCO centrifugal filter (Cat #: UFC803024, Millipore Sigma, Burlington, MA) TEV protease in 25 mM HEPES buffer ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 3 days at 37°C before overlay medium was removed and cells were fixed with 4% PFA (Sigma-Aldrich, MA) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Lung homogenates were clarified of debris by centrifugation at 800 xg for 5 minutes then aliquots were frozen at −80°C neat for plaque assay or diluted at a 1 to 4 ratio in TRIzol (Sigma-Aldrich, MA) for RNA isolation and quantitative PCR ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was transferred to 0.45 µm nitrocellulose membranes prior to overnight incubation at 4°C with the following primary antibodies: mouse anti-ATXN3 (1H9; 1:1000, MAB5360; Millipore, Billerica, MA), mouse anti□GAPDH (1:10000 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins purified under native conditions were concentrated to 400 μL using a 10 kDa molecular weight cutoff (MWCO) Amicon Ultra-4 centrifugal spin concentrator (Merck-Millipore, Burlington, MA) and purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (Cytiva) ...
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bioRxiv - Bioengineering 2023Quote: ... Molecular mass was validated using a MicroFlex MALDI-TOF (Bruker, Billerica, MA) using α-cyano-4-hydroxycinnamic acid as the matrix (Sigma-Aldrich). Peptides were purified to ≥ 95% on a VYDAC reversed-phase c18 column attached to a Waters 2487 dual λ adsorbable detector and 1525 binary HPLC pump (Waters ...