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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for 4 ArmPEG MA 10K since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The supernatant (extra-synaptic fraction) was concentrated using an Amicon Ultra 15 10K (#UFC901008, Merck Millipore, Ireland) and protein was precipitated with six volumes of acetone at -20 °C and recovered by centrifugation for 30 minutes at 18,000 g ...
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bioRxiv - Biochemistry 2021Quote: ... concentrated to less than 500 μl in a 10K cut off Ultrafiltration Unit (Sigma Cat. No. UFC801024) for a final step of gel filtration over a Superdex 75 column (Sigma-Aldrich Cat ...
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bioRxiv - Immunology 2020Quote: ... 40 μl of plasma were loaded in an Amicon®Ultra 10K centrifugal filter (UFC501096 EMD Millipore), diluted with pure water to 500 μl ...
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bioRxiv - Molecular Biology 2023Quote: ... PsC23 protein-containing fraction was collected and imidazole was removed by 10K centrifugal filter (Merck Millipore, Germany) using buffer (20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2023Quote: ... the purified native protein was first concentrated to 8 mg/ml by ultrafiltration (Amicon Ultracel-10K, Millipore), then mixed with 2 mM ATP ...
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bioRxiv - Bioengineering 2023Quote: Conditioned medium was harvested and then concentrated 10-fold using a 10K cutoff spin concentrator (EMD Millipore). 4X non-reducing sample buffer (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... Fractions containing target protein were desalted and concentrated on Amicon Ultra 10K centrifugal filter units (Merck Millipore), and the final protein preparation was stored in PBS containing 0.05% Triton X-100 at 4°C.
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bioRxiv - Biochemistry 2023Quote: ... the reaction buffer was exchanged to the lysis buffer five times by Amicon Ultra 10K (Merck Millipore). Then ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were seeded (∼10K cells/cm2) on either 50-100 μg/ml poly-D-lysine (Sigma, P0899) plus laminin (Thermo ...
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bioRxiv - Neuroscience 2021Quote: ... using a purified mouse monoclonal O-4 antibody (1:50, MAB345, Millipore, MA, USA) for 12 h at 4°C after blocking for 30 min at room temperature with 5% donkey serum (S30-100ml ...
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bioRxiv - Cell Biology 2022Quote: ... FTractin cDNA was fused with Halo tag in pTriEx-4 vector (Millipore Sigma, MA). EGFP was swapped with mEos3.2 41 in the cortactin-EGFP vector to obtain cortactin-mEos3.2 ...
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bioRxiv - Bioengineering 2023Quote: ... all slides (patterns and controls) were fixed with 4% formaldehyde (Millipore Sigma, Burlington, MA) for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... at 4 °C in a 6–30% OptiPrep (Sigma-Aldrich, MilliporeSigma, Burlington, MA, USA) density gradient ...
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bioRxiv - Biophysics 2021Quote: ... and DNAJB1 chaperone variants were concentrated on an Amicon Ultra-15 10K molecular weight cutoff (MWCO) filter (Millipore) and further purified on a HiLoad 16/600 Superdex 200 pg gel filtration column (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... The peak fractions were concentrated by Amicon ultra-15 centrifugal filtration (MW 10K cut off; Millipore, Temecula, CA) and stored at −80°C.
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bioRxiv - Molecular Biology 2021Quote: ... Fabs were concentrated up to ~1 mg/mL using an Amicon Ultra 0.5 filter (10k cut-off, Millipore) and conjugated with CF640 or Cy3 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... The elution was concentrated to about 10 ml by ultrafiltration using Amicon Ultra with MWCO of 10K (Millipore), and 80 μl of PreScission protease was added per 10 mg of PI5P4Kβ ...
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bioRxiv - Systems Biology 2021Quote: ... we plated 10k – 30k cells on 12 mm collagen-coated glass coverslips (rat tail collagen, Sigma, #122-20). Cells were plated the afternoon prior to imaging ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... FGD was removed by spin filtration in a 0.5-mL 10K molecular-weights cutoff (MWCO) spin filter (Millipore), and F420H2 was used within 1 h of FGD being removed ...
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bioRxiv - Cell Biology 2020Quote: ... Peak fractions of target proteins were collected and concentrated using Amicon Ultra-15 10K Centrifugal filters (Sigma-Aldrich).
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bioRxiv - Biochemistry 2021Quote: ... The PCR products of control and sort2 were concentrated by using Amicon Ultra-0.5 10K (UFC501096, Merck Millipore), and analyzed by pair-end sequencing using NovaSeq 6000 system (Illumina) ...
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bioRxiv - Neuroscience 2023Quote: ... which contained glycerol and was purified before metal conjugation with Amicon 10K Buffer Exchange Columns (UFC501096, EMD Millipore). All antibody concentrations were obtained using a Nanodrop 2000c Spectrophotometer and formulated with a final stock concentration of 0.5mg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated to 10 mL using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore) and loaded into SnakeSkin Dialysis Tubing ...
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bioRxiv - Microbiology 2023Quote: ... whereas KCl was increased to 200 mM for Ppk) using an Amicon Ultra 10K centrifugation device (Merck Millipore). Glycerol was added to 20% (v/v ...
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bioRxiv - Synthetic Biology 2023Quote: The purified antibody-EndoTag fusions were subsequently concentrated in a 10K MWCO Amicon Ultra centrifugal filter unit (Millipore) and polished via SEC using a Hiload 26/600 Superose 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The recovered RNAs were diluted with RNase-free water and concentrated using the 10K centrifugal filters (Merck Millipore) and subsequently stored at-20℃.
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bioRxiv - Microbiology 2020Quote: ... and TIGR4 (serotype 4) were inoculated onto tryptic soy agar (TSA) (GranuCult-Millipore Burlingon MA) plates supplemented with 3% sheep blood (iTek StPaul MN ...
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bioRxiv - Cell Biology 2022Quote: Confluent hRECs in 0.1% gelatin-coated EZ slide 4-well chamber (Millipore, Burlington, MA, USA) were pretreated with either scrambled or KAI peptides in serum starvation media for 2 hr ...
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bioRxiv - Biochemistry 2023Quote: ... The coverslips were then mounted in Mowiol 4-88 solution (Calbiochem/EMD Millipore, Billerica, MA) and fluorescence analysis was performed using a Plan-Apochromat 63×/1.4 objective (Zeiss ...
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bioRxiv - Neuroscience 2020Quote: ... then fixed in 4% paraformaldehyde (FisherScientific, Cat#50-980-493, Waltham, MA) in PBS with 0.3% Triton X-100 (Millipore Sigma, Cat#X100, Burlington, MA) (PBST ...
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bioRxiv - Molecular Biology 2020Quote: ... The fractions containing ORF24-NTD-Strep were pooled and concentrated on a 10K Amicon Ultra-15 concentrator (EMD Millipore). Protein aliquots were flash frozen and stored at -70°C.
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bioRxiv - Biochemistry 2020Quote: ... Protein pellets were resuspended in 120 μL PBS containing 1% SDS and desalted by passing through Amicon Ultra 0.5 mL 10K cutoff desalting columns (Merck Millipore) equilibrated with 1% NP-40 ...
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bioRxiv - Microbiology 2021Quote: ... Fractions containing each DNA pol of a high purity were pooled and concentrated using a 10K protein concentrator (Millipore). Concentrated proteins were preserved at −20 °C in the presence of 50% glycerol ...
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bioRxiv - Biophysics 2022Quote: ... Eluted fractions corresponding to the full ternary complex were gathered and spun concentrated to 14.5 mg/mL using an Amicon Ultrafree 10K NMWL Membrane Concentrator (Millipore). Crystals were grown 1-3 µL hanging drops by varying the ratio of protein to mother liquor from 0.5-2:0.5-2 respectively ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial lysis was done by enzymatic cell wall degradation (30 min at 37°C with 10K/ml mutanolysine, Sigma) followed by mechanical disruption (FastPrep-24 ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were filtered with Sterile Syringe filter (0.22 μM) and concentrated with Millipore Amicon Ultra-15 10K (Merck Millipore) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2020Quote: ... The purified covalent protein-DNA complexes were concentrated to about 0.1– 0.2 mM using Ultracel®-10K Centrifugal Filters (Millipore) in a buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing recombinant PKAc verified via SDS-PAGE were pooled and concentrated to 250μL using an Amicon concentrator (10K MWCO; Millipore). The concentrated sample was then applied to a preparative-grade Superdex200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... pooled together and concentrated down to ∼2 mL with an Amicon Ultra 10K MWCO centrifugal filter unit (Millipore UFC901024). The HIS6-SUMO-tag was cleaved by proteolysis with 1 mg TEV protease for 2 hours at 4°C in the cold room ...
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bioRxiv - Biochemistry 2023Quote: ... pooled together and concentrated down to 0.5 mL with a Amicon Ultra 10K MWCO centrifugal filter unit (Millipore UFC901024). Finally ...
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bioRxiv - Biophysics 2022Quote: ... CM was concentrated ≈300 times to 0.5 mL using a Centricon Plus-70 centrifugal filter device with a 10K nominal molecular weight limit (Merck Millipore).
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bioRxiv - Biophysics 2022Quote: ... Fraction 4-6 of three different columns were pooled and concentrated to 0.5 mL using an Amicon Ultra-15 10K filter (Merck Millipore). This was then placed on top of the gradient.
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bioRxiv - Immunology 2023Quote: ... The diluted reaction mixture was then purified by UF/DF using 10K MWCO PES membrane (Millipore Pellicon 2 Mini) against PBS buffer (30X ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde (PFA) and permeabilized with 0.1% SDS (Merck Millipore, Burlington, MA). Primary antibodies were incubated for 1 h at RT a humid chamber ...
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bioRxiv - Biochemistry 2020Quote: ... and anti-integrin β1 antibody (clone HUTS-4) was obtained from Chemicon (Merck Millipore, Billerica, MA). Rabbit polyclonal antibodies to fibronectin and fibrinogen were from Abcam (Cambridge ...
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bioRxiv - Neuroscience 2024Quote: ... Amicon Ultra-4 centrifugal filters with 100 kDa molecular weight cutoff (Merck Millipore, Burlington, MA, USA) along with 0.22 μM Nalgene® syringe filter units (sterile ...
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bioRxiv - Neuroscience 2023Quote: ... Amicon Ultra-4 centrifugal filters with 100,000 Da molecular weight cutoff (Merck Millipore, Burlington, MA, USA) along with 0.22 µM Nalgene® syringe filter units (sterile ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions containing dCas9 protein were pooled and the protein was concentrated with spin concentrators (Amicon Ultra 15, MWCO 10k; Millipore), and after adding glycerol to 20% final concentration ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then concentrated by centrifuging at 5000 RPM in an Amicon® Ultra-15 10K centrifugation column (Millipore). Protein concentration was measured by UV absorbance at 280nm using an Ultraspec 2100 pro by Amersham Biosciences ...
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bioRxiv - Plant Biology 2021Quote: ... The eluate containing the nanodiscs was concentrated to 1 ml with the use of an Amicon Ultra-15 10K device (Merck Millipore) and then mixed with 10 ml of translation buffer containing 30 mM HEPES-KOH (pH 7.8 ...