Labshake search
Citations for Millipore Sigma :
201 - 250 of 10000+ citations for 4 ArmPEG MA 10K since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: Conditioned cell culture medium was collected and concentrated 20-fold by using Amicon Ultra-0.5 mL 10K MWCO Centrifugal Filters (Millipore Sigma). LOX activity was measured with an Amplite Fluorimetric Lysyl Oxidase Assay kit (AAT Bioquest ...
-
bioRxiv - Plant Biology 2021Quote: ... Cleaved His-tagged GB1 and remaining TEV protease were removed via a second Ni-NTA purification after buffer exchange to HEPES buffer containing 40 mM imidazole using an Amicon Ultra-10K centrifugal filter (Merck Millipore). The tag-free protein was subjected to SEC using a Superdex S75 Increase 10/300 column equilibrated in HEPES buffer without imidazole and a pH of 7.5 ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
-
bioRxiv - Microbiology 2020Quote: ... Purified proteins were diluted to a final concentration of 50 mM NaCl and then concentrated on Amicon centrifugal filters 30K for FHL and 10K for LIM domains (Millipore). The proteins’ purities and identities were confirmed by SDS-PAGE and mass spectrometry ...
-
bioRxiv - Biochemistry 2020Quote: BCCIP was buffer exchanged to 25 mM Tris-HCl (pH 7.5) and 1 mM DTT in an Amicon Ultra-0.5 (Millipore, 10k cutoff) and concentrated to 15 mg/ml (514 μM) ...
-
bioRxiv - Immunology 2021Quote: SARS-CoV-2 or MERS-CoV RBD protein was exchanged into 0.01 M PBS [pH 7.2] buffer using centrifugal filter units (10k MWCO; Millipore Amicon Ultra). A 5-fold molar excess of SM(PEG)24 (PEGylated ...
-
bioRxiv - Biochemistry 2022Quote: ... Sample was concentrated using an Amicon Ultra 10K molecular weight cut-off centrifugal filter device (Merck Millipore, Billerica, Massachusetts, USA) up to 8 mg/ml ...
-
bioRxiv - Biochemistry 2022Quote: ... Cleaved His-tagged GB1 and remaining TEV were removed via a second Ni-NTA purification after buffer exchange to Buffer A using an Amicon Ultra-10K centrifugal filter (Merck Millipore). The tag-free protein was subjected to SEC using a Superdex S200 Increase 26/600 column equilibrated in HEPES buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Biophysics 2022Quote: ... 2 mL of EV-containing eluate was collected and concentrated to 100 μL using Amicon Ultra-2 10K filters (Merck Millipore).
-
bioRxiv - Neuroscience 2023Quote: ... Culture supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
-
bioRxiv - Biochemistry 2023Quote: ... uncleaved protein and TEV were subsequently removed by an ortho nickel affinity purification step and the sample concentrated to < 2 mL using a 10K Amicon Ultra-15 concentrator (Millipore). Finally ...
-
bioRxiv - Immunology 2023Quote: ... EVs were further concentrated and unincorporated MemGlow 700 dye was removed by centrifugation with Amicon 15 Ultra RC 10k Da MWCO filters (EDM Millipore). PalmGRET EVs not labeled with MemGlow 700 were also further concentrated after TFF on Amicon 15 Ultra RC 10 kDa MWCO filters ...
-
bioRxiv - Biophysics 2023Quote: ... Conditioned media were concentrated at the ratio of 30x using Amicon Ultra centrifugal filter units with 10k MWCO (Millipore Sigma). Concentrated conditioned media normalized to cell numbers were loaded on 4-15 % SDS-PAGE gels (Bio-Rad ...
-
bioRxiv - Biochemistry 2023Quote: ... The eluted holo-McyA2-(C-A-PCP) protein was pooled and concentrated to 15 mg/ml by ultrafiltration (Amicon Ultracel-10K, Millipore), then ...
-
bioRxiv - Synthetic Biology 2024Quote: ... protein samples were concentrated by ultrafiltration using Amicon ultra centrifugal filter devices with a molecular weight cut-off of 10K (Millipore) and dialyzed in PBS (pH 7.5 ...
-
bioRxiv - Neuroscience 2024Quote: ... and recombinant protein isolated by GST column purification and buffer exchanged into PBS using Amicon Ultra 10K MWCO centrifugal filters (Millipore). Recombinant proteins were quantified using a BCA assay (Pierce ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The sample was then buffer exchanged to 100 mM Tris pH 7 buffer using an Amicon® Ultra 0.5 mL 10K Centrifugal Filter (Millipore) and concentrated to 2 mg/mL ...
-
bioRxiv - Biochemistry 2024Quote: ... followed by concentrating using an Amicon Ultra-15 Centrifugal Filter Unit (Regenerated Cellulose 10,000 NMWL Ultracel-10K Millipore, Cat. #UFC901024) for 15 min intervals at 3,500 rpm at 4 °C until a final volume between 100 and 500 μL was achieved.
-
bioRxiv - Biochemistry 2024Quote: ... The appropriate fractions were pooled and the complex was concentrated to ∼ 9 mg/ml using Amicon UltraCel 10K MWCO centrifugal filters (Merck Millipore).
-
bioRxiv - Biophysics 2024Quote: ... Cushions were fractionated and fractions containing the assembled array were pooled and concentrated down using a 10K centrifugal filter (Ammincon Ultra, 0.5m, Millipore UFC501024). Arrays were concentrated to >3μM nucleosomes and stored at 4°C for immediate use or at −80°C for up to 6 months.
-
bioRxiv - Cell Biology 2020Quote: ... We found that an additional surface treatment of 4 µg/cm2 human plasma Fibronectin (Millipore, Billerica MA) in DMEM applied overnight at 37°C with mild orbital plate agitation was necessary to create a consistent surface coating for cell adhesion ...
-
bioRxiv - Immunology 2022Quote: ... eluted with 250 mM imidazole diluted in PBS followed by up-concentration and buffer-exchange to PBS using Amicon® Ultra-15 10K Centrifugal Filter Units (Merck Millipore). Monomeric fractions were isolated by size exclusion chromatography as described above for the ACE2-albumin fusion protein ...
-
bioRxiv - Cell Biology 2023Quote: ... The unbound portion of this capture step was kept as HIS-free eGFP protein and conjugates and concentrated once on a 10K Amicon column (Millipore Sigma) by spinning for 6-8 minutes at 6000x g ...
-
bioRxiv - Immunology 2023Quote: Serum autoantibodies were quantified using the Luminex xMAP platform (Luminex) with the MILLIPLEX MAP Human Autoimmune Autoantibody Panel (HAIAB-10K, Millipore Sigma) following the manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2024Quote: ... Protein fractions were buffer-exchanged into 50 mM Tris-HCl (pH8.0) containing 100 mM NaCl and 10% glycerol using an Amicon Ultra-0.5 centrifugal filter 10K device (Merck Millipore, Darmstadt, Germany).
-
bioRxiv - Biophysics 2022Quote: ... HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl] ethanesulfonic acid) was purchased from EMD Millipore (Billerica, MA USA) and NaCl (sodium chloride ...
-
bioRxiv - Bioengineering 2022Quote: ... Sliced sections were permeabilized and blocked with 0.1% Tween-20 and 4% donkey serum (Merck Millipore, Burlington, MA) in Tris-buffered saline ...
-
bioRxiv - Microbiology 2021Quote: The anti-flavivirus E monoclonal antibody (mAb) D1-4G2-4-15 was purchased from Millipore (Billerica, MA, USA). The rabbit anti-ZIKV E polyclonal antibody was purchased from GeneTex (Alton PkwyIrvine ...
-
bioRxiv - Neuroscience 2020Quote: ... Proteins were electrophoretically separated on 4–12% SDS-PAGE gels and transferred to a PVDF membrane (Millipore, MA). Membranes were subsequently blocked with blocking buffer (Sigma ...
-
bioRxiv - Immunology 2021Quote: ... After exactly 10 minutes the beads were removed from the F(ab’)2-dilution by filtration in Spin-X tube filters (Costar®) and the filtrate was concentrated in Amicon® Ultra Filters (10k, Millipore). Purified F(ab’)2 fragments were analysed by SDS-PAGE.
-
bioRxiv - Immunology 2021Quote: ... After exactly 10 minutes the beads were removed from the F(ab’)2-dilution by filtration in Spin-X tube filters (Costar®) and the filtrate was concentrated in Amicon® Ultra Filters (10k, Millipore). Purified F(ab’)2 fragments were analysed by SDS-PAGE.
-
bioRxiv - Cell Biology 2022Quote: ... Pooled eluates were then concentrated approximately 40 times using a centrifugal filter (Amicon Ultra-2 10k, UFC201024, Merck Millipore, Billerica, Massachusetts, USA) following the manufacturers’ instructions ...
-
bioRxiv - Microbiology 2023Quote: ... 700 µL supernatant was transferred to a new 1.5-mL tube and concentrated using Amicon Ultra 1 mL 10K membrane filters (Millipore, catalog number: UFC501096) to ≈40 µL by centrifuging at room temperature at 14,000 x g for ≈45 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... pooled and concentrated to 11 mg/ml using a 10-kDa cut-off centrifugal filter (Amicon® Ultra-15 10K, Millipore #UFC901096), flash-cooled in liquid nitrogen and stored at -80°C.
-
bioRxiv - Molecular Biology 2020Quote: ... and incubated for 16h at 4°C with 2µL α-H3K27me3 antibody (#07-449; Merck Millipore, Burlington, MA, USA) or α-H3K4-me3 antibody (Rabbit monoclonal antibody (#04–745 ...
-
bioRxiv - Molecular Biology 2021Quote: ... eluate was concentrated to ~300 uL using 4 Amicon Ultra 0.5 mL 10 kDa centrifugal filters (Millipore, Billerica, MA).
-
bioRxiv - Biochemistry 2020Quote: ... The protein sample was concentrated by ultrafiltration (Amicon Ultra-4 centrifugal filter devices, 50,000 MWCO; Millipore, Billerica, MA, USA), and the solution was changed to buffer A ...
-
bioRxiv - Neuroscience 2021Quote: ... slices were incubated overnight at 4°C in mouse anti-NeuN primary antibody (#MAB377, RRID:AB_2298772, Millipore Sigma, Billerica, MA) at a 1:1000 concentration in diluted blocking solution (10% fetal plus ...
-
bioRxiv - Biochemistry 2023Quote: ... The dialyzed protein solution was then concentrated using Amicon Ultra-4 Centrifugal Filter Units 30 K (Millipore, MA #UFC803024) according to manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... Peak fractions were pooled and concentrated to 500 μl using Amicon Ultra-15 10K MWCO centrifugal filters (Merck Millipore UFC901008; final LDAO = 2%) and further purified by size-exclusion chromatography as described for human VDAC constructs but with 20 mM HEPES pH 7.6 ...
-
bioRxiv - Neuroscience 2021Quote: ... and overnight at 4°C in the rabbit polyclonal antibody directed against calretinin (1:300, Millipore, Burlinghton, MA, USA; AB5054) diluted in PBS containing 0.3% Triton X-100 and 1% NDS ...
-
bioRxiv - Bioengineering 2021Quote: ... 4 mL of MP suspension was placed in a centrifugal filter unit (MWCO 10,000, Amicon® Ultra, Millipore, MA, USA) and centrifuged at 8,000 rpm for 1 h to separate the un-entrapped PEITC ...
-
bioRxiv - Bioengineering 2021Quote: ... Liposomal purification was accomplished with Amicon Ultra-4 centrifugal filter units with a 100kDa MWCO (Millipore Sigma, Burlington, MA, USA) to eliminate free 7,8-DHF in the mixture ...
-
bioRxiv - Neuroscience 2022Quote: The MILLIPLEX® MAP human amyloid beta and tau magnetic bead panel 4-plex ELISA kit (Millipore, Burlington, MA, USA) and the Bio-Plex 200 System instrument (Biorad ...
-
bioRxiv - Neuroscience 2021Quote: ... Following electrophoresis, proteins were transferred (100 V, 2 hr, 4°C) to polyvinylidene difluoride membranes (Immobilon-FL, Millipore, Billerica, MA). To confirm protein transfer ...
-
bioRxiv - Microbiology 2023Quote: ... The tPA fluorogenic substrate D-Val-Leu-Lys 7-amido-4-methylcoumarin (10 μM) (Millipore-Sigma, Burlington, MA, USA, #A8171) was added ...
-
bioRxiv - Microbiology 2023Quote: ... The tPA fluorogenic substrate D-Val-Leu-Lys 7-amido-4-methylcoumarin (10 μM) (Millipore-Sigma, Burlington, MA, USA, #A8171) was added ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... anti-total-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor (anti-tAMPAR) (Cat.#AB1504, Merck Millipore, Burlington, MA, USA), anti-phospho (Ser845)-AMPAR (anti-pAMPAR;cat.#AB5849 ...
-
bioRxiv - Immunology 2024Quote: ... High-binding 96-well microtiter plates (Costar, Cambridge, MA) were coated overnight at 4°C with a goat anti-human IgG Fc antibody (Sigma) at 3 µg/mL in Carbonate-Bicarbonate buffer (0.1 M ...
-
bioRxiv - Neuroscience 2024Quote: ... Wings were mounted on microscope slides in Canadian Balsam Fir (1.01691.0025, CAS-No: 8007-47-4, Sigma Aldrich, MA, USA), coverslipped and sealed with nail polish ...