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Citations for Millipore Sigma :
2801 - 2850 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 500 µM N-acetyl-L-cysteine (NAC, # A7250, Sigma-Aldrich), 0.3 µM Erastin (#E7781 ...
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bioRxiv - Microbiology 2024Quote: ... The 1-N-phenylnaphthylamine (NPN) was purchased from Sigma-Aldrich, USA ...
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bioRxiv - Cancer Biology 2021Quote: ... was prepared in 2% vitamin E/d-a-tocopheryl polyethylene glycol 1000 succinate (Sigma) immediately prior to IV administration by tail vein injection at 25 mg/kg ...
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bioRxiv - Bioengineering 2020Quote: ... hematoxylin and eosin (H&E) staining was performed using Harris hematoxylin solution (Sigma-Aldrich) and eosin solution (Electron Microscopy Sciences) ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were stained with Hematoxylin and Eosin (H&E) and Goldner’s trichrome (Sigma-Aldrich). Images were obtained with Lionheart LX (Biotek Instruments Inc ...
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bioRxiv - Microbiology 2021Quote: ... substrate and the positive control E-64 (Sigma Aldrich, St. Louis, MO, Unites States) were prepared at 10 mM in DMSO ...
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bioRxiv - Neuroscience 2020Quote: ... The mixed glial cells were plated on poly-D-lysine (PDL, #A003-E, Millipore) coated 10 cm dishes and cultured in DMEM medium (#1196092 ...
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bioRxiv - Immunology 2021Quote: ... then rehydrated and stained with hemoxtylin & eosin (H&E) (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Pathology 2020Quote: Lungs were sectioned and stained with both Hematoxylin/Eosin (H&E) (Sigma Aldrich, USA) and Masson Trichrome (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... B16 Melanoma cells or R1/E ESCs were fixed with 10% formalin (Sigma; HT501128) and incubated at 370C for 10 minutes ...
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bioRxiv - Immunology 2020Quote: ... followed by the addition of the TMB/E ELISA substrate (Merck Millipore, Billerica, MA). Absorbance was measured as described.
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated in poly-D-lysine (50 μg/ml, Millipore A-003-E) coated flasks and grown in astrocyte growth media containing DMEM (Gibco 11960044) ...
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bioRxiv - Biochemistry 2021Quote: ... coverslips were coated with 0.05 mg/ml Phaseolus vulgaris erythroagglutinin (PHA-E) lectin (Sigma) and incubated in a humid environment for 15 min at 37°C ...
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bioRxiv - Physiology 2021Quote: ... The basal media utilized in all conditions consisted of Williams E medium (Sigma #W1878), PenStrep ...
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bioRxiv - Bioengineering 2019Quote: ... sericata maggot E/S was assessed using a Protease Fluorescent Detection Kit (Sigma Aldrich), This kit detects the activity of serine ...
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bioRxiv - Microbiology 2022Quote: ... 10 and 25µg mL−1 of human purified E-cadherin (5085, Sigma-Aldrich, USA) in DMEM or DMEM without FBS as control for 1 h at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were stained with H&E and 2% Alizarin Red solution (all Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... and E-cadherin at 1:100) in binding buffer (0.2% Triton-X100 (Sigma, X100), 2.2% gelatin (Sigma ...
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bioRxiv - Physiology 2022Quote: ... stained with hematoxylin and eosin (H&E) and mounted with Canada balsam (Sigma-Aldrich). The sections were examined on the Nikon ECLIPSE Ni-U microscope with Digit Sight DS-Fi2 digital camera (Nikon ...
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bioRxiv - Cell Biology 2024Quote: ... at 37 °C overnight and then coated with extracellular matrix (ECM) (E-1270, Sigma) at 4 °C for at least 6 h ...
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bioRxiv - Neuroscience 2024Quote: ... the cell suspension was transferred to a poly-D-lysine (PDL, #A003-E, Millipore) pre-coated T-75 flask and maintained in DMEM/F12 (#11320033 ...
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bioRxiv - Biochemistry 2023Quote: ... Dehydroepiandrosterone-2,2,3,4,4,6-D6 (D6-DHEA) and cortisone-2,2,4,6,6,9,12,12-D8 (D8-E) were purchased from Sigma Aldrich, while 11-ketoandostenedione-2,2,4,6,6,9,12,12,16,16-D10 (D10-11KA4 ...
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bioRxiv - Cancer Biology 2023Quote: ... paraffin-embedded sections (10 um) were stained with hematoxylin & eosin (H&E, Sigma-Aldrich), mouse anti-human CD3 (DAKO) ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were stained with Haematoxylin and Eosin (H&E) and TRAP (all Sigma Aldrich). For H&E ...
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bioRxiv - Neuroscience 2023Quote: ... 3 formula hematoxylin and eosin Y (H&E, Sigma Aldrich, St. Louis, MO, USA) and toluidine blue (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... and water for H&E staining were purchased from Sigma Aldrich (St Louis, MO). Histological-grade xylenes were purchased from Spectrum Chemical ...
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bioRxiv - Cell Biology 2024Quote: ... at 37 °C overnight and then coated with extracellular matrix (ECM) (E-1270, Sigma) at 4 °C for at least 6 h.
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bioRxiv - Cancer Biology 2024Quote: ... 4 µm sections were stained with H&E (Sigma-Aldrich, St. Louis MO, USA), digitized (Nanozoomer ...
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bioRxiv - Molecular Biology 2019Quote: ... that amplify the coding sequence of the phytoene desaturase gene (tetur01g11270) (Table S1. PCRs were performed using the Expand Long Range dNTP Pack (Roche/Sigma-Aldrich, Belgium). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The binding of VHH-Fcs/ACE2-Fc to S1 was detected using 100 µL 1 µg/mL HRP-conjugated goat anti-human IgG (Sigma, Cat#A0170). Finally ...
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bioRxiv - Microbiology 2020Quote: ... and susceptible (all MIC≤ 1mg/L) strains (S1 to S4) were incubated in cation adjusted Mueller Hinton II Broth II (MHB2, Sigma-Aldrich, USA) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to S1 and RBD antigen was detected using a three-step approach consisting of anti-mouse or antirat Fc biotin (Sigma Aldrich B7401) followed by streptavidin-HRPO (Life Technologies cat# SA1007 ...
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bioRxiv - Cell Biology 2022Quote: ... and an N-hydroxysuccinimide-fibronectin solution prepared by combining 1 part N-hydroxysuccinimide solution (cat. no. A8060, Sigma-Aldrich; 1 mg/ml in dimethyl sulfoxide) and 9 parts fibronectin solution (cat ...
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... the glass microelectrodes were heated to 300°C on a hot plate and silanized in an atmosphere of N,N-dimethyltrimethylsilylamine (Sigma Aldrich, Saint Louis, MO, USA) under an inverted ...
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bioRxiv - Molecular Biology 2021Quote: SARS-CoV-2 was diluted to the desired amounts in RDM supplemented with 100 μM of taurine (Sigma-Aldrich) without FBS ...
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bioRxiv - Microbiology 2021Quote: ... and a SARS-CoV-2 reporter vector (50 ng) and 1.5 μL of X-tremeGENE 9 transfection reagent (Sigma) and incubated for 48 hrs ...
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bioRxiv - Microbiology 2020Quote: ... HCoV-NL63 and SARS-CoV-2 were supplemented with 1 µg/ml TPCK-treated trypsin (Sigma-Aldrich, cat. #T1426) increasing the rate of infection ...
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bioRxiv - Immunology 2020Quote: ... pTT3-SARS-CoV-2-S was transfected into 293E cells using 293 Free transfection reagent (EMD Millipore Cat # 72181) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... were cultured in 24 well plates and stimulated with inactivated SARS-COV-2 antigen (1.2 μg/ml) or PMA (25 ng/ml, cat # P8139; Sigma) and Ionomycin (1 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 S expression HEK293T cells were stained with PKH26 dye according to the manufactures protocol (Sigma-Aldrich). Next ...
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bioRxiv - Immunology 2022Quote: ... the cells were immunostained with a mouse monoclonal antibody against SARS-CoV-1/2 nucleoprotein [clone 1C7C7 (Sigma-Aldrich)] ...
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bioRxiv - Microbiology 2023Quote: Whole lungs were isolated from SARS-CoV-2 infected mice and homogenized in 600 μL TRIzol (Sigma-Aldrich, MA) with 3.2 mm stainless steel beads (NextAdvance ...
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bioRxiv - Genetics 2021Quote: ... Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis, Mo). Bleomycin hydrochloride was from Nippon Kayaku (Tokyo ...
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bioRxiv - Immunology 2020Quote: ... Both C3d constructs were expressed in the Escherichia coli BL21(DE3) (Sigma Aldrich) or Shuffle T7 (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... coli by co-expression of all subunits from three plasmids: pRSFDuet-1 (Novagen) encoding a HiS7-SUMO-TEV-Rrn9 fusion and Rrn10 ...
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bioRxiv - Molecular Biology 2020Quote: ... overexpression of GST-β1a was carried out using Escherichia coli BL21 (DE3) (Novagen) transformed with plasmid containing the cDNA inserted into pGEX-4T-2 vector ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli cell-free TXTL extract was prepared from BL21 Rosetta 2 from Novagen, as described previously.23,24 The TXTL system is commercially available as the product “myTXTL” from Arbor Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmids encoding for IGPD were transformed into Escherichia coli BL21 (DE3) cells (Novagen) and protein expression was induced for 5 h with 1 mM isopropyl-b-D-thiogalactopyranoside at 37°C in Luria broth ...
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bioRxiv - Biophysics 2020Quote: ... The plasmid was transformed in Escherichia coli Rosetta™ (DE3) pLysS cells (Novagen). Selected colonies were grown in luria broth supplemented with 25 μg/ml chloramphenicol and 25 μg/ml kanamycin at 37 C until an OD between 0.6-0.8 was reached ...
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bioRxiv - Bioengineering 2021Quote: ... or lipopolysaccharides (LPS) from Escherichia coli (200 µg/mL, Sigma Aldrich, Cat# L4391) for 24 hours before the permeability test ...