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Citations for Millipore Sigma :
2601 - 2650 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... All protein constructs were expressed in Escherichia coli BL21 (DE3) cells (Novagen) with a Tobacco Etch Virus (TEV ...
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bioRxiv - Biochemistry 2019Quote: ... The kinase domains were expressed in Escherichia coli ROSETTA (DE3) cells (Novagen) with TEV cleavable N-terminal GST and a non-cleavable C-terminal His6-tag ...
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bioRxiv - Microbiology 2019Quote: ... the plasmids were transformed into Escherichia coli Rosetta pLys-S cells (Novagen) for expression of the protein.
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bioRxiv - Microbiology 2019Quote: ... Lipopolysaccharides from Escherichia coli O111:B4 (Sigma-Aldrich Japan Inc., Tokyo, Japan) for the TLR-4 cell line and Pam3CSK4 and Zymozan (Novus Biologicals ...
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bioRxiv - Biochemistry 2021Quote: ... The Mpro construct was transformed into Escherichia coli strain Rosetta(DE3) (Novagen) and transformed clones were pre-cultured at 37 °C for 5 h in lysogeny broth supplemented with appropriate antibiotics ...
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bioRxiv - Biochemistry 2021Quote: Plasmids were transformed into chemically competent Escherichia coli BL21(DE3) cells (Novagen) and used to inoculate TB medium supplemented with 100 µg/mL of ampicillin or 50 µg/mL of kanamycin for pGEX-6P-1 or pET28b vectors ...
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bioRxiv - Bioengineering 2021Quote: ... 25 ng/ml lipopolysaccharides (LPS) from Escherichia coli O111:B4 (Millipore Sigma) or 25 ng/ml Interleukin-4 (IL-4 ...
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bioRxiv - Microbiology 2020Quote: ... coli Rosetta2 DE3 pLysS BL21 derivative cells (Sigma-Aldrich, ST. Louis, MO) were grown at room temperature in auto-inducing media according to Studier et al ...
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bioRxiv - Plant Biology 2020Quote: OsDMI3-GST fusion protein was produced in Escherichia coli Rosetta (DE3; Novagen), and tested for interaction as described previously (Ni et al. ...
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bioRxiv - Cell Biology 2021Quote: ... or 1 μg/ml LPS (from Escherichia coli strain 0127:B8, Sigma) (all Peprotech unless noted) ...
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bioRxiv - Biophysics 2022Quote: ... and ASH2L proteins were individually expressed in Escherichia coli (Rosetta II, Novagen) and purified as described previously (66) ...
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bioRxiv - Physiology 2020Quote: ... coli LPS (100 μg; O55:B5, Sigma-Aldrich, Castle Hill, NSW, Australia) or intravenous E.coli LPS (1 μg/kg infused over one hour ...
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bioRxiv - Biophysics 2019Quote: ... coli MG1655 genome by PCR into pET15b (Merck Millipore, Billerica, MA, USA) by Gibson assembly (New England Biolabs ...
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bioRxiv - Biochemistry 2019Quote: ... Selenomethionine substituted proteins were overexpressed in Escherichia coli B834 (DE3) cells (Novagen) using PASM-5052 autoinduction media (Studier 2005) ...
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bioRxiv - Microbiology 2020Quote: ... coli strain BL21 (DE3) (86) was grown on Mueller Hinton agar (Sigma) in bioassays ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5α and purified using the GenElute Miniprep kit from Sigma-Aldrich.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Lipopolysaccharides from Escherichia coli O111:B4 (LPS) were obtained from Sigma-Aldrich Cat# 2630 ...
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bioRxiv - Genetics 2021Quote: ... coli using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich, St. Louis, MO) or the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... coli EF-G was cloned into a pET24a+ vector (Novagen, kanamycin resistance), and the plasmid was transformed into E ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells (Rosetta2(DE3) for YFPnano and BL21(DE3) (EMD Millipore Novagen) for T4Lnano ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells (Rosetta2(DE3) for YFPnano and BL21(DE3) (EMD Millipore Novagen) for T4Lnano ...
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bioRxiv - Immunology 2021Quote: ... injected with 15 mg/kg LPS (Escherichia coli O111:B4, Sigma, MO). Mice were i.p ...
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bioRxiv - Microbiology 2022Quote: ... coli was determined utilizing the Cell Proliferation Kit I (Milipore Sigma #11465007001). A suspension containing a MOI 50 of each strain was added to a confluent monolayer of host cells (T24 ATCC or HK2 ATCC ...
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bioRxiv - Molecular Biology 2022Quote: ... and neutrophils stimulated by LPS (LIPOPOLYSACCHARIDES FROM ESCHERICHIA COLI, Sigma, L4391-1MG) in concentration 1 µg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... Canada) and transformed in Escherichia coli BL21 (DE3) (Novagen/Merck, Darmstadt, Germany) for protein production ...
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bioRxiv - Biochemistry 2022Quote: ... coli Rosetta (DE3) and purified using glutathione CL-4B Sepharose (Sigma 49739). His-FBXW7/Skp1 was a gift from Frauke Melchior (University of Heidelberg ...
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bioRxiv - Microbiology 2022Quote: ... Nanobodies were expressed in Escherichia coli Rosetta-gami 2 (DE3) cells (Novagen) in autoinduction media and purified through consecutive nickel affinity chromatography and size-exclusion chromatography (see Supplementary Methods).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lipopolysaccharide (LPS) from Escherichia coli O55:B5 (Sigma Aldrich, St. Louis, USA), and playa or dust collected from various sites in the region near the Salton Sea (0.025-0.100 g/L) ...
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bioRxiv - Neuroscience 2024Quote: ... LPS from Escherichia coli O55:B5 (Sigma Aldrich, St. Louis, MO, USA), was chosen here for its ability to induce inflammation54 following 0.5-ml weekly injections containing 0.1 mg/kg LPS ...
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bioRxiv - Immunology 2023Quote: ... 10 ng/mL lipopolysaccharides from Escherichia coli O127:B8 (Sigma Aldrich, L3129), 40 ng/mL human recombinant tumour necrosis factor alpha (Peprotech ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were transformed into Escherichia coli BL21 Tuner (DE3) strain (Sigma-Aldrich) or Rossetta DE3 (Novagen ...
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bioRxiv - Microbiology 2023Quote: ... coli) on filters with a pore size of 0.22 μm (Merck Millipore) for 24 hours at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... and orally gavaged with LPS from Escherichia coli O111:B4 (Sigma, L3024) in water or olive oil ...
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bioRxiv - Microbiology 2023Quote: ... coli DH5⍺ was grown in 25 mL Luria-Bertani media (Sigma-Aldrich) at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... coli cells was resuspended in 50.0 μL of 1X TE (Sigma-Aldrich). The cells were lysed and solubilised with 2000 U of Ready-Lyse Lysozyme (Epicentre ...
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bioRxiv - Biochemistry 2023Quote: ... coli was cloned into the first multiple cloning site of pRSFDuet (Novagen) with an N-terminal glutathione S-transferase (GST ...
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bioRxiv - Genetics 2023Quote: ... the Cas9 protein was expressed in BL21 Rosetta Escherichia coli strain (Novagen) in magic medium at 37 °C for 12 h followed by 24 h at 18 °C ...
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bioRxiv - Bioengineering 2023Quote: ... coli 0111:B4 (LPS) was purchased from Sigma-Aldrich (St. Louis, MO). Raw Blue™ cells ...
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bioRxiv - Biochemistry 2023Quote: ... coli GyrA coding sequence was cloned in pACYC-Duet (from Millipore/Novagen) under the T7 promoter ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng/ml of LPS from Escherichia coli O26:B6 (Sigma-Aldrich) was added to appropriate wells for additional 2 h or 24 h ...
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bioRxiv - Biochemistry 2023Quote: ... coli GyrA coding sequence was cloned in pACYC-Duet (from Millipore/Novagen) under the T7 promoter ...
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bioRxiv - Genetics 2024Quote: ... coli OP50 were spotted with 25µL 1mg/mL thapsigargin in DMSO (Sigma) or DMSO only directly on to the E ...
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bioRxiv - Plant Biology 2024Quote: ... MBP fusion proteins were expressed in Escherichia coli Rosetta (DE3) pLysS (Novagen) and purified using Amylose Resin (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: Codon substitutions were introduced into the vector pBE-S/yukChis by site-directed mutagenesis using complementary pairs of oligonucleotides bearing the desired substitution (listed among others in Table S1, synthesized by Sigma-Aldrich) and the Pfu Turbo DNA polymerase (Agilent technologies) ...
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bioRxiv - Microbiology 2021Quote: ... approximately 50 - 100 L of groundwater was filtered sequentially through 0.2 µm and 0.1 µm pore sized PTFE filters (142 mm, Omnipore Membrane, Merck Millipore, Germany; Table S1). With the exception of H32 (did not yield sufficient volumes) ...
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bioRxiv - Immunology 2021Quote: The sequence of SARS-CoV-2 S1 was obtained from the cloned full-length S sequence and was cloned into the expression vector pTriEx1.1 (EMD Millipore, UK) and characterised as described previously [12] ...
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bioRxiv - Microbiology 2019Quote: ... Primers (Table S1) used for PCR amplification of the beta-lactams genes (blaTEM, blaSHV, blaCXT-M and blaOXA) were acquired from Sigma Aldrich, USA ...
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bioRxiv - Genomics 2021Quote: ... K562 cells were electroporated with Cas9 RNP against target site 1 (Table S1) and treated with mirin (Millipore Sigma, M9948-5MG) 20 minutes after electroporation at 50 µM final concentration ...
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bioRxiv - Biochemistry 2022Quote: ... jannaschii genomic DNA (procured from ATCC) using primers listed in Table S1 and cloned into two tandem multiple cloning sites of pET-Duet vector (Novagen, Merck), using the restriction enzymes BamHI and SalI for MjFHα subunit ...
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bioRxiv - Genomics 2022Quote: ... culture medium was replaced with S1-media (Final 11,6 g/L MCDB131, Sigma Aldrich, M8537-1L; 2 mM D-+-Glucose, Sigma Aldrich, G7528-250G ...