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Citations for Millipore Sigma :
2801 - 2850 of 10000+ citations for Estrone 3 Sulfate E1S ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: The ELISPOT plate (Millipore, MSIPS4W10) was filled with 35% ethanol at 50ul/well for 1min ...
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bioRxiv - Biochemistry 2023Quote: ... 0.45-μM filter plates (Millipore). The cells were PK digested (5 μg/ml ...
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bioRxiv - Immunology 2023Quote: Multiscreen ELISpot white plates (Millipore) were coated overnight at 4°C with the anti-mouse IFNγ AN18 antibody (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were maintained on antibiotic water for two weeks (Polymyxin B sulfate salt, Sigma-Aldrich P1004; NeoMycin trisulfate salt hydrate, Sigma-Aldrich N1876). Mice were monitored for onset of disease by investigators and animal care personnel.
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Mutant ACTB mRNA 3′UTR Promotes Hepatocellular Carcinoma Development by Regulating miR-1 and miR-29abioRxiv - Cancer Biology 2019Quote: Lysates of individual cells (10 μg / lane) were separated by sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE, 10 %) electrophoresis and transferred onto nitrocellulose membranes (Millipore Corporation, Billerica, MA). The membranes were then incubated in a SuperBlock T20 PBS Blocking Buffer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Molecular Biology 2020Quote: ... were separated by 8% and 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) before transferred to PVDF membranes (Millipore, Billerica, MA, USA). The membranes were blocked with 5% skim milk compound with TBST for 3h and incubated with corresponding primary antibodies and appropriate secondary antibodies before the protein bands were detected with ECL-chemiluminescent kit (ECLplus ...
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bioRxiv - Genetics 2022Quote: ... The metallothionein promoter in the pMET-GAL4 driver (Velichkova et al. 2010) is activated by addition of copper sulfate (CuSO4, Sigma-Aldrich Nr. 451657), to a final concentration of 0.75 mM ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amount of lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride membrane (Merck Millipore, Burlington, MA, USA) in 25 mmol/L Tris ...
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bioRxiv - Physiology 2020Quote: ... were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). For western blotting ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were separated through electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel and then transferred onto an Immobilon-P membrane (Merck Millipore, Burlington, MA, USA). After blocking with 5% bovine serum albumin ...
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bioRxiv - Molecular Biology 2020Quote: ... were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to Polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, MA, USA). Membranes were then blocked for 90 min with specific 5% non-fat dried milk and incubated with primary detection antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were maintained on antibiotic water for two weeks (Polymyxin B sulfate salt, Sigma-Aldrich P1004; NeoMycin trisulfate salt hydrate, Sigma-Aldrich N1876). Mice were monitored for onset of disease by investigators and animal care personnel.
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bioRxiv - Physiology 2022Quote: ... The protein extracts were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to Immobilon membrane (EMD Millipore, Billerica, MA). The membrane was blocked with 5% nonfat milk for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein samples were then separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by transfer to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). The membranes were first blocked with 5% skim milk for 1 hour and then incubated with the primary antibodies of SARS-CoV-2(2019-nCoV)Spike RBD Antibody (Sinobiology ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA). The antibodies are listed in Supplementary Table 4 ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... We directly replaced this solution with hybridization buffer with the same composition plus 10% w/v dextran sulfate (Sigma Aldrich, cat. no. D8906) and the three FISH probes ...
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bioRxiv - Cell Biology 2023Quote: ... bacteria were transformed by heat shock (30 sec, 42 °C) and colonies were selected overnight using 50 µg/mL kanamycin sulfate (Sigma-Aldrich, Darmstadt, Germany). Some N-terminal deletion mutations were cloned by PCR using Phusion Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: Treated cell and tissue lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) for separation and were then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, MA, USA), which were incubated with the appropriate primary antibodies (all diluted 1:1000 in 1.5% bovine serum albumin (BSA) ...
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bioRxiv - Neuroscience 2023Quote: ... were subjected to 5% or 13% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (PVDF, Millipore, Bedford, MA, USA). The following primary antibodies were used in this study ...
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bioRxiv - Neuroscience 2024Quote: ... The polyester solution in acetonitrile was slowly added drop by drop into the vigorously stirred aqueous sodium dodecyl sulfate (SDS) (Sigma Aldrich, Cat# L3771) solution at 1700 rpm ...
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bioRxiv - Cancer Biology 2019Quote: ... the gel was blotted and probed with antisense telomere probe (CCCTAA)8 labelled with DIG Oligonucleotide 3’-End labeling Kit (Sigma-Aldrich) or with a 1.6 kb fragment containing the sequence (TTAGGG)n62 ...
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bioRxiv - Biochemistry 2022Quote: K562 cell line derivatives were collected by centrifugation at 1400 x g for 3 mins and nuclei were isolated using the Nuclei EZ Prep nuclei isolation kit (Sigma- Aldrich). The nuclei were fixed in 4% (v/v ...
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bioRxiv - Genetics 2022Quote: ... The external ammonium concentration was estimated as a function of time for 3 hours using the below-mentioned principle (Ammonia Assay Kit - Sigma-Aldrich). At each time point ...
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bioRxiv - Cell Biology 2023Quote: ... Working stocks were screened to ensure the absence of mycoplasma every 3 months using LookOut Mycoplasma PCR Detection Kit (Cat. No. D9307 Sigma, UK). Cells were cultured in Modified Eagle’s Medium supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: ... Serum samples were collected from surviving mice and 3 samples from each group were used for estimation of Creatine Kinase activity Assay Kit (Sigma-Aldrich).
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bioRxiv - Bioengineering 2023Quote: ... the samples were first homogenized and hydrolyzed in concentrated hydrochloric acid (HCl) at 120°C for 3 hours before being quantified using hydroxyproline assay kit (Sigma-Aldrich). A microplate reader (Promega GloMax ...
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bioRxiv - Developmental Biology 2024Quote: ... or ear biopsies (3-week-old pups) was used for PCR based genotyping using the REDExtract-N-Amp kit (Sigma-Aldrich) with the primers listed in STable5 ...
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bioRxiv - Developmental Biology 2021Quote: IGF1 levels were measured in serum and SSPC culture supernatant using ELISA (Sigma-Aldrich, RAB-0229) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... CCL2 and CCL5 protein levels were quantified using a sandwich ELISA procedure (Sigma, Cat# RAB0055 & RAB0077) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... as well as BG505 Env-NP expression by ELISA (NP capture: Galanthus nivalis lectin (GNL, Sigma) / NP detection ...
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bioRxiv - Immunology 2020Quote: ... The pro-inflammatory cytokines in the supernatant were analyzed by multiplex ELISA (Millipore, HCYTMAG-60K-PX29) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: Plasma cytokines and cytokines secreted in cell culture media were measured using ELISA (Luminex 200; Millipore) by following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... sandwich ELISA of tau-5 coating antibody (20 µg/ml; Millipore Cat# 577801-100UG RRID: AB_212534) with either biotin-conjugated HT-7 (for human tau ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed in ELISA wash buffer (0.01M PBS with 0.05% Tween 20; Sigma; P7949) for 4x 2 minutes on an orbital shaker ...
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bioRxiv - Developmental Biology 2022Quote: ... 3-dpf larvae were anesthetized with 0.016 % Tricaine/Ethyl 3-aminobenzoate methanesulfonate salt (A5040, SIGMA-ALDRICH) and microinjected with 5-8 nL of liposome encapsulated clodronate (SKU# CLD-8909 ...
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bioRxiv - Microbiology 2019Quote: ... EVs were incubated with Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) at different final concentration (0 to 10 µg/ml) ...
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bioRxiv - Plant Biology 2021Quote: ... Roots samples were vacuum infiltrated with 3-3’-diaminobenzidine tetrahydrochloride (1.25 mg/mL, DAB, Sigma-Aldrich), Tween-20 (0.05% v/v ...
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bioRxiv - Pathology 2024Quote: ... concentrated 10 times using Amicon Ultra-4 Ultracel-3 3 kDa centrifugal filter units (Millipore, UFC800308). Aliquots of concentrated media and flowthrough media were frozen at −80°C.
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bioRxiv - Pathology 2024Quote: ... concentrated 10 times using Amicon Ultra-4 Ultracel-3 3 kDa centrifugal filter units (Millipore, UFC800308). Aliquots of concentrated medium and flowthrough medium were frozen at −80°C.
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bioRxiv - Cell Biology 2023Quote: ... sections were washed 3 times with 0.01% PBS-T and DAPI (Sigma-Aldrich, #28718-90-3) nuclear stain ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... 3’-3’ linkage of CpG with some staples were applied by dibenzocyclooctyne (DBCO)-azide (Sigma, US) click chemistry (Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured in medium supplemented with 2 mM 3-phosphoglycerate (3-PG, Sigma-Aldrich, #P8877) or 2 mM serine (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... gels were washed 3 times x 5 minutes with a 3% Bovine Serum Albumin (BSA, Sigma) blocking solution in PBS ...
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bioRxiv - Microbiology 2023Quote: ... blocked for one hour in blocking buffer (3% goat serum and 3% BSA in PBS (Sigma)) and incubated with pneumococcal antisera Pool Q (SSI Diagnostica ...
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bioRxiv - Cell Biology 2023Quote: ... Bottom coverslips are activated with a 2% 3-Aminopropyltrimethoxysilane (3-APTMS) (Sigma Aldrich 13822-56-5) in 96% ethanol solution for 5 minutes and washed with 70% ethanol ...
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bioRxiv - Synthetic Biology 2024Quote: ... cat # K3007) and 3-O-C12-HSL (N-(3-Oxododecanoyl)-L-homoserine lactone from Sigma-Aldrich, cat # O9139 ...
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bioRxiv - Molecular Biology 2021Quote: ... plates were incubated with a 1:20,000 dilution of anti-FLAG M2 HRP-conjugated monoclonal antibody (Sigma-Aldrich) in blocking buffer for another 1 h at RT ...
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bioRxiv - Synthetic Biology 2019Quote: MDA-MB-231 cells were cultured to 80% confluency on coverslips in 6-well plates and stained with 200µL complete DMEM containing 1µL DiO at a concentration of 1 mM (Sigma) to visualise the cell membrane ...
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bioRxiv - Microbiology 2019Quote: SH-SY5Y cells were seeded into 24-well plates treated with poly-D-lysine (1 mg/mL; Sigma). When cells reached 90% confluence ...