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Citations for Millipore Sigma :
2751 - 2800 of 10000+ citations for Estrone 3 Sulfate E1S ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... containing pre-equilibrated preimplantation embryo culture media supplemented with 1 µM PDO325901 and 3 µM CHIR99021 (Sigma-Aldrich). Embryos were grown one more day in NDiff 227 media (Takara ...
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bioRxiv - Cell Biology 2021Quote: To measure symplasmic connectivity using the HPTS (8-Hydroxypyrene-1, 3, 6-trisulfonic acid trisodium salt, SIGMA-ALDRICH) dye movement assay ...
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bioRxiv - Microbiology 2019Quote: ... multi-tube mixer for 3 min before adding one volume of phenol:chloroform:isoamyl alcohol (25:24:1) (Sigma-Aldrich). Samples were mixed vigorously ...
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bioRxiv - Microbiology 2020Quote: ... and 200 μM of a non-selective family-wide phosphodiesterase inhibitor 3-Isobutyl-1-methylxanthine (IBMX; Sigma, #I5879) in a mix of DMEM/F-12 medium (50/50 ...
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bioRxiv - Neuroscience 2020Quote: To label endogenous RNAs neurons were washed once with cold PBS with 3 μg.ml-1 digitonin (Sigma Aldrich), once with 50% methanol in PBS and fixed in cold 100% methanol for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Appropriate fractions were concentrated to 3-0.7 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Cell Biology 2020Quote: DNA was precipitated with 1 volume of isopropanol and 0.1 Volume of 3 M sodium acetate (pH 5.2, Sigma) at −80°C for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Total RNA of the macrophages was extracted by the phenol/1-bromo-3-chloropropane method (Sigma-Aldrich, UK) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μM 1-[2-[[(Diphenylmethylene)imino]oxy]ethyl]-1,2,5,6-tetrahydro-3-pyridinecarboxylic acid hydrochloride hydrochloride (NO711) (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was resuspended and incubated for 30 min at 4 °C in buffer B (10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 0.5% IGEPAL CA-630, 10% Glycerol, 1 mM DTT, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail) ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected using a 3:1 polyethylenimine (PEI [branched average Mw ∼25,000 Da; Sigma-Aldrich]) to DNA ratio (30:10 μg ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Neuroscience 2022Quote: ... we delivered the Iκ-kinase inhibitor [5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide (TPCA-1; Sigma-Aldrich CAS 507475-17-4 ...
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bioRxiv - Cell Biology 2022Quote: ... or 10nM GIP in stimulation buffer (1x Hanks Buffered Saline Solution, 0.1% BSA, 0.1% 3-isobutyl-1-methylxanthine (IBMX) (all Sigma), 0.5mM HEPES (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: D1R agonist 2,3,4,5,-tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine hydrochloride (SKF38393; Sigma-Aldrich, St Louis, MO) was dissolved in sterile saline at a concentration of 0.5 μg/μL ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed with 1× PBS and blocked in 3% BSA with 0.1% Triton-X 100 (Thermo-Fisher) and 0.1% Tween (Sigma) in PBS for one hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 3 ×104 THP-1 cells were seeded and differentiated by the addition of 5 ng/ml PMA (Sigma) in complete RPMI media (Himedia ...
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bioRxiv - Biochemistry 2022Quote: ... The final supernatant was concentrated to > 1 mg/ml using a 3 kDa centrifugal filter unit (Millipore Sigma) and frozen in aliquots stored at −80°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed with 1× PBS and blocked in 3% BSA with 0.1% Triton-X 100 (Thermo-Fischer) and 0.1% Tween (Sigma) in PBS for one hour at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... and blocked for 1h with 3% goat serum and 1% BSA in PBS/0.25% Triton X-100 (Sigma). The primary antibodies were incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and blocked for 1 hour at RT in 3% BSA (Applichem) in PBS-Tween buffer (0.01%) (ThermoFisher, Sigma). The membranes were then incubated overnight at 4°C with primary antibodies listed in the Supplemental table 3 with agitation ...
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bioRxiv - Biophysics 2023Quote: ... Samples were prepared in fully deuterated 400 mM SPB for all measurements and contained ∼1 mM 3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid (Sigma-Aldrich) as a frequency reference.
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bioRxiv - Cell Biology 2024Quote: ... in 3% (w/v) BSA were added for 1 hour before addition of 300 nM DAPI (Sigma Aldrich) and ProLong® Gold Antifade (Cell Signaling Technology) ...
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bioRxiv - Biochemistry 2024Quote: ... SidK(1-278)-3×FLAG (∼0.85 mg) was bound to 1.5 mL of M2-FLAG affinity gel resin (Sigma) and flow through was recirculated onto the column 6-7 times ...
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bioRxiv - Biochemistry 2023Quote: ... and phosphatase inhibitors (prepared in-house according to Phosphatase inhibitor cocktail 1, 2 and 3 from Sigma-Aldrich)) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with 10 μM of the agonist N-[(3R)-1-Azabiciclo[2.2.2]oct-3-yl]furo[2,3-c]pyridine-5-carboxamide (PHA-543613, from Sigma, PZ0135) for 30 min ...
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bioRxiv - Bioengineering 2024Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and collagenase type 1 was purchased from Sigma-Aldrich. RNA extraction kit (Iran) ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were maintained on antibiotic water for two weeks (Polymyxin B sulfate salt, Sigma-Aldrich P1004; NeoMycin trisulfate salt hydrate, Sigma-Aldrich N1876). Mice were monitored for onset of disease by investigators and animal care personnel.
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Mutant ACTB mRNA 3′UTR Promotes Hepatocellular Carcinoma Development by Regulating miR-1 and miR-29abioRxiv - Cancer Biology 2019Quote: Lysates of individual cells (10 μg / lane) were separated by sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE, 10 %) electrophoresis and transferred onto nitrocellulose membranes (Millipore Corporation, Billerica, MA). The membranes were then incubated in a SuperBlock T20 PBS Blocking Buffer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Molecular Biology 2020Quote: ... were separated by 8% and 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) before transferred to PVDF membranes (Millipore, Billerica, MA, USA). The membranes were blocked with 5% skim milk compound with TBST for 3h and incubated with corresponding primary antibodies and appropriate secondary antibodies before the protein bands were detected with ECL-chemiluminescent kit (ECLplus ...
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bioRxiv - Genetics 2022Quote: ... The metallothionein promoter in the pMET-GAL4 driver (Velichkova et al. 2010) is activated by addition of copper sulfate (CuSO4, Sigma-Aldrich Nr. 451657), to a final concentration of 0.75 mM ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amount of lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride membrane (Merck Millipore, Burlington, MA, USA) in 25 mmol/L Tris ...
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bioRxiv - Physiology 2020Quote: ... were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). For western blotting ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were separated through electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel and then transferred onto an Immobilon-P membrane (Merck Millipore, Burlington, MA, USA). After blocking with 5% bovine serum albumin ...
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bioRxiv - Molecular Biology 2020Quote: ... were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to Polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, MA, USA). Membranes were then blocked for 90 min with specific 5% non-fat dried milk and incubated with primary detection antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were maintained on antibiotic water for two weeks (Polymyxin B sulfate salt, Sigma-Aldrich P1004; NeoMycin trisulfate salt hydrate, Sigma-Aldrich N1876). Mice were monitored for onset of disease by investigators and animal care personnel.
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bioRxiv - Physiology 2022Quote: ... The protein extracts were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to Immobilon membrane (EMD Millipore, Billerica, MA). The membrane was blocked with 5% nonfat milk for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein samples were then separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by transfer to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). The membranes were first blocked with 5% skim milk for 1 hour and then incubated with the primary antibodies of SARS-CoV-2(2019-nCoV)Spike RBD Antibody (Sinobiology ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA). The antibodies are listed in Supplementary Table 4 ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... bacteria were transformed by heat shock (30 sec, 42 °C) and colonies were selected overnight using 50 µg/mL kanamycin sulfate (Sigma-Aldrich, Darmstadt, Germany). Some N-terminal deletion mutations were cloned by PCR using Phusion Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: Treated cell and tissue lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) for separation and were then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, MA, USA), which were incubated with the appropriate primary antibodies (all diluted 1:1000 in 1.5% bovine serum albumin (BSA) ...
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bioRxiv - Neuroscience 2023Quote: ... were subjected to 5% or 13% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (PVDF, Millipore, Bedford, MA, USA). The following primary antibodies were used in this study ...
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bioRxiv - Cancer Biology 2023Quote: ... We directly replaced this solution with hybridization buffer with the same composition plus 10% w/v dextran sulfate (Sigma Aldrich, cat. no. D8906) and the three FISH probes ...
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bioRxiv - Neuroscience 2024Quote: ... The polyester solution in acetonitrile was slowly added drop by drop into the vigorously stirred aqueous sodium dodecyl sulfate (SDS) (Sigma Aldrich, Cat# L3771) solution at 1700 rpm ...
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bioRxiv - Immunology 2021Quote: ... multiscreen HA plates (Millipore, MAHAS4510) were coated with purified NP at 1 μg/ml in PBS overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: Millipore ELISPOT plates (Millipore Ltd) were coated with anti-IFN-γ capture Ab (Cellular Technology Ltd ...
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bioRxiv - Immunology 2021Quote: ... ELISpot plates (Millipore cat # MAHAS4510) were coated overnight at 4°C with 2.5 μg/ml of P ...
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bioRxiv - Microbiology 2021Quote: ... wells of ELISpot plates (Millipore) were coated overnight with purified soluble pre-F protein (30 ng/well in PBS) ...
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bioRxiv - Genomics 2021Quote: ... using filter plates (Millipore #MSGVN22) for acidification and washes ...