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Citations for Millipore Sigma :
2701 - 2750 of 10000+ citations for Estrone 3 Sulfate E1S ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... lapachol (2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthoquinone) were purchased from Sigma-Aldrich. Vismione H and madagascine were obtained from PGE2 fraction of Psorospermum glaberimum as previously described (Gallé ...
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bioRxiv - Molecular Biology 2022Quote: ... home-made LIF conditioned medium and 2i inhibitors: 3 μM GSK-3 inhibitor XVI (Sigma) and 10 μM MEK inhibitor PD0325901 (Tocris)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Oligonucleotides 5’-GAAAAAAAAAATATACGCTAAGATTTTTGG-3’ and 5’-ATGACTAAACCCCCCCTCC-3’ synthesized and HPLC-purified by Sigma-Aldrich were used ...
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bioRxiv - Microbiology 2023Quote: ... Crypts were treated from seeding on with 3-hydroxyanthranilic acid (3-HAA; 200µM; Sigma-Aldrich).
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bioRxiv - Physiology 2024Quote: ... CDH1 Forward 5′-TTACTGCCCCCAGAGGATGA-3′ and Reverse 5′-TGCAACGTCGTTACGAGTCA-3′;) were purchased from Sigma-Aldrich. mRNA expression was determined using the comparative 2-ΔΔCt method and normalized to the mRNA expression level of endogenous references (PPIA ...
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bioRxiv - Molecular Biology 2024Quote: ... 5’-GATCCCCAAATCT-3’ and 5’-GATCAGAT[BtndT]TGGG-3’ with 5’ end phosphate (Sigma-Aldrich), were annealed (81 µl of each Oligo 100 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... 5’-GATCCCCAAATCT-3’ and 5’-GATCAGAT[BtndT]TGGG-3’ with 5’ end phosphate (Sigma-Aldrich), were annealed (81 µl of each Oligo 100 µM ...
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bioRxiv - Bioengineering 2020Quote: ... or covalently crosslinked with 75 mM of dihydrazide (AAD)/1-hydroxybenzotriazole (HOBT) with 100 mg/mL of 1-ethyl-3- (dimethylaminopropyl) carbodiimide (EDC) (all from Sigma-Aldrich, St. Louis, MO) (Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 g pectin,0.25 g xylan, 1 g starch, 3 g yeast extract, 1 g proteose peptone, 2 g pig gastric mucin; all from Sigma Aldrich, St. Louis, MO). The amounts of emulsifiers to add were calculated to obtain concentrations of 0.005% (m/v) ...
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bioRxiv - Physiology 2024Quote: ... for 10 days and treated with 100 nM endothelin-1 (ET1) or vehicle (H2O) the last 3 days as previously described.25 Cardiomyocytes were then stained for α-actinin (Sigma, SAB2108642, 1:800) and N-cadherin (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Permeabilization buffer was removed and washed 3×2 minutes before blocking for 1 hour at room temperature in a 1% Bovine Serum Albumin (BSA) (Millipore Sigma, cat: 2910-OP) in DPBS solution ...
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bioRxiv - Biophysics 2023Quote: ... was purchased from MedChem-Express (Monmouth Junction, NJ, USA). The phospholipid 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC, see Fig. 1) was purchased from Sigma-Aldrich (Merck KGaA, Germany). Potassium bromide (KBr) ...
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bioRxiv - Neuroscience 2023Quote: ... 215 uL of either ice-cold 20mM Tris-HCl/2 mM MgCl2/0.5% Triton X-100 with protease inhibitor (Pierce #A32953) and phosphatase inhibitor cocktail 3 (Sigma #P0044; Buffer #1, Bf#1) or boiling 1%SDS/50mM NaF (Buffer #2 ...
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bioRxiv - Molecular Biology 2024Quote: ... then fully resuspended in 1 mL cold resuspension buffer supplemented with 1% each phosphatase inhibitor cocktails 2 and 3 (Sigma Cat #P5726 and P0044). To lyse the resuspended cells ...
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bioRxiv - Biochemistry 2020Quote: ... 1 µl of L-Glutamate Dehydrogenase (Ammonia kit, Sigma), 2 µl of 150 mM NAD+ ...
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bioRxiv - Molecular Biology 2020Quote: 4.5×106 PlatE cells were seeded in gelatin-coated 10cm plates (0.1% gelatin, Millipore) the day before transfection ...
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bioRxiv - Neuroscience 2024Quote: Multi-well plates (12 wells, polystyrene plate, flat bottom. Sigma-Aldrich, cat. no. CLS3512)
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bioRxiv - Cancer Biology 2021Quote: Tail tip fibroblasts were seeded in 96-well plates and the mitochondrial membrane potential (MMP) was determined using the mitochondrial membrane potential kit (Sigma) following the recommendations made by the manufacturer ...
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bioRxiv - Plant Biology 2022Quote: ... thaliana seedlings for each line grown on a ½ MS agar plate using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The same protocol was used when the fluorescence reporter was substituted by a luminescence readout in this case we utilize 96 well white cell-culture plates and the Firefly Luciferase Assay kit from Sigma following the manufacturer protocol.
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bioRxiv - Biochemistry 2023Quote: ... the samples were further treated in a 96-well plate following the “Coenzyme A Assay Kit” protocol from Sigma-Aldrich. The absorbance of the colorimetric product (570 nm ...
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bioRxiv - Immunology 2023Quote: ... Neisserial genomic DNA was extracted from cell suspensions of overnight culture plates using the GenElute Bacterial Genomic DNA Kit (Sigma). Genomic libraries were generated using Illumina Nextera DNA Flex Library Prep according to the manufacturer’s instructions and sequenced on Illumina MiSeq 2×250bp paired end platform ...
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bioRxiv - Cell Biology 2020Quote: ... glass-bottomed dishes were first cleaned and activated with 1% v/v (3-Aminopropyl) triethoxysilane (APTES, Sigma-Aldrich) in ethanol ...
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bioRxiv - Neuroscience 2021Quote: ... 3 ml of an enzyme cocktail [1 mg/ml collagenase A (#11088793001, Sigma-Aldrich, St. Louis, MO, USA) and 0.1 mg/ml elastase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... shShank3 injected mice were randomly infused with 2µL (500nL m-1) of vehicle (∼3 % dimethyl sulfoxide (DMSO, Sigma) diluted in aCSF ...
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bioRxiv - Plant Biology 2020Quote: ... low-fat dry milk) or anti-GFP (Sigma-Aldrich, dilution 1:1000 in TBS-T with 3% BSA) primary antibody at 4°C overnight ...
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bioRxiv - Cancer Biology 2019Quote: Cells were treated with blebbistatin (20μM, 3 hr) (Selleckchem, S7099), fasudil (5μM, 24 hr) (Selleckchem, S1573), ML7 (20μM, 1 hr) (Sigma, I2764), ionomycin (10μM ...
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bioRxiv - Plant Biology 2021Quote: ... 250 μM or 1 mM L-glutamic acid monosodium salt monohydrate (Sigma Aldrich, cat No. 6106-04-3). Plants were analyzed in single blinded-experiments ...
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bioRxiv - Biochemistry 2021Quote: ... 3 nM of detergent-solubilized or nanodisc-reconstituted complex I and either 1 mM FeCy (Sigma Aldrich BVBA) or 100 μM Q1 (Sigma Aldrich BVBA ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... and construct expression was induced for 3 days using media above with 1 µg/mL tetracycline (Sigma Aldrich) added ...
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bioRxiv - Biochemistry 2020Quote: ... Japan) and 1:3 mixture of PVA and polyvinylpyrrolidone (PVP: average MW, 40,000; Sigma-Aldrich, St. Louis, MO), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was exposed to donkey anti-mouse IgG conjugated to Cy-3 in PB (1:80; EMD Millipore) for 1 h for visualization of Hu ...
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bioRxiv - Neuroscience 2020Quote: ... counterstained with DAPI (diluted 1:1000 in PBS) for 3 minutes at RT and mounted with Fluoromont (Sigma).
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bioRxiv - Microbiology 2021Quote: ... and the sequences of the siRNAs targeting DDX42 were siDDX42-1: 5’-CAGAAUGCCUGGUUUCGGA-3’ (SASI_Hs01_00119846, Sigma-Aldrich®), siDDX42-2 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting liquid was aliquoted into 4 phase lock tubes (Quanta Biosciences) and extracted 3 times with an equal volume of phenol:chloroform:isoamylalcohol (25:24:1, Sigma). A final extraction with an equal volume of chloroform (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... followed by staining of cell nuclei for 3 min at RT with 1 μg/ml Hoechst 33342 (Sigma) in PBS−/− ...
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bioRxiv - Microbiology 2020Quote: ... blocked for 1 h in PBS with 3% normal goat serum and 0.5% bovine serum albumin (BSA; Sigma), and incubated for 1 h with anti-VP1 rabbit serum (1:200 ...
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bioRxiv - Cell Biology 2021Quote: The gRNA complex was prepared by mixing 1 μl of tensin 3 crRNA (100 μM; AGUC-CGCUCCCGCUCAUAG, SIGMA) and 1 μl of trRNA (100 μM ...
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bioRxiv - Cell Biology 2021Quote: ... (3) 30 μL casein wash buffer (30 mM DTT, 1 mg/mL filtered casein (Sigma-Aldrich, C5890-500G) in BRB80 pH 6.8) ...
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bioRxiv - Cell Biology 2020Quote: ... for 3 hr to induce mCherry–FokI expression and 1 mg/ml doxycycline hyclate (D9891–G, Sigma–Aldrich) for an additional 3 hr to induce reporter gene transcription ...
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bioRxiv - Biochemistry 2022Quote: ... 0.02% Triton X-100 and blocked for 1 hour in a 3% Bovine Serum Albumin (BSA, Sigma Aldrich), 0.02% Triton X-100 blocking solution ...
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bioRxiv - Bioengineering 2022Quote: ... 3 the cell culture media was replaced 1-2 hours before imaging with RPMI media (Sigma-Aldric R8755) supplemented with B27 ...
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bioRxiv - Bioengineering 2022Quote: ... After blocking for 1 h by adding a drop of 3 % bovine serum albumin (A9418, Sigma-Aldrich, USA) onto exposed tissue ...
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bioRxiv - Developmental Biology 2020Quote: ... for 3 minutes and incubated at room temperature for 30 minutes in 1% Alcian Blue 8GX (Sigma-Aldrich) in 3% acetic acid solution ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: Mice were fasted for 3 h and injected with Triton WR-1339 (1 mg/g BW, Sigma-Aldrich) via the tail veins ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately1-2 μL of endotoxin-free DNA (1-3 mg/ml) diluted in PBS/0.025% Fast Green (SIGMA) was injected into the lateral ventricles of the forebrain using heat-pulled glass micropipettes (Drummond) ...
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bioRxiv - Microbiology 2022Quote: ... LAM was resuspended at 1 mg/mL in sterile nanopure water (Direct-Q 3 UV-R, Millipore Sigma). 60-μL aliquots were stored in low-retention tubes at –80 °C and thawed immediately prior to use ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected using a 3:1 polyethylenimine (PEI [branched average Mw ~25,000 Da; Sigma-Aldrich]) to DNA ratio (30:10 μg ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were then washed in PBTD for 3×10 min and 1mg/ml DAPI (1:250, Sigma-Aldrich) was added and incubated for 10 minutes between the first and second washes ...