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Citations for Millipore Sigma :
2401 - 2450 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The fixed cells were washed three times with 0.1% Triton X-100 (Sigma Aldrich, Cat# X100) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then washed three times and finally immersed in DABCO (1,4-diazabicyclo [2.2.2]octane, Sigma, Cat# D27802 ...
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bioRxiv - Genetics 2024Quote: ... The excised gel band was destained three times with 50 mM ammonium bicarbonate (Sigma-Aldrich, A6141) and 50% methanol (Fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... both times for 8-12 minutes and in the presence of DNase I solution (Sigma Aldrich). FBS (Cytiva HyClone ...
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bioRxiv - Neuroscience 2024Quote: ... Analytes were identified by matching retention time to known standards (Sigma Chemical Co., St. Louis MO.) and compounds were quantified by comparing peak areas to those of standards on the dominant sensor.
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bioRxiv - Genomics 2024Quote: ... About 5 μL of nuclei were diluted 10 times and stained with DAPI (Sigma Cat. D9542). The nuclei quality and density were evaluated with a hemocytometer under a microscope ...
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bioRxiv - Molecular Biology 2024Quote: ... at the time of collection SUF tissue was rinsed twice in Hank’s Balanced Salt Solution (Sigma) followed by mincing in high glucose DMEM (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed three times with DPBS supplemented with 0.1 % Tween 20 (Sigma-Aldrich, #P7949). In the next staining step ...
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bioRxiv - Physiology 2024Quote: ... Samples were washed (3 times, 5 min each) with PBS containing 0.1% Triton X100 (Sigma-Aldrich), and then incubated with secondary antibody (Table 1 ...
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bioRxiv - Neuroscience 2024Quote: ... the glyoxal was removed and the samples were washed three times with PBS (D8662, Sigma-Aldrich) for five minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Then slides were washed 2x in PLA Wash Buffer A for 5 min and ligation solution according to the manufacturer’s instructions was added (Duolink® In Situ Detection Reagents FarRed, Sigma-Aldrich, DUO92013). The ligation was performed at 37C for 30 minutes followed by 2 washes PLA Wash Buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 hours cells were fixed with 4% PFA and processed for isPLA using Duolink® In Situ Detection Orange (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Formalin-fixed and paraffin-embedded lung sections from mock and IAV-infected mice were prepared as previously described (44) and probed for epithelial or endothelial cells detection using anti-mouse αSMA (1:10,000; Sigma-Aldrich, Darmstadt, Germany), rabbit anti-mouse CD31 (1:50 ...
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bioRxiv - Pathology 2019Quote: ... Tissues were removed from the Leica Bond and incubated overnight at 37°C with hybridization solution (20 nM RNA detection probes per target; 100 μg/mL denatured, sonicated salmon sperm DNA (Sigma-Aldrich, D7656); 2.5% dextran sulfate (Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... Near-infrared Western immunoblot using Odyssey CLx detection system (Li-COR) was performed on the purified complexes with the following antibodies: Arp3 (Sigma A5979, Mouse), ARPC5L (Abcam ab169763 ...
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bioRxiv - Cell Biology 2021Quote: ... Tubulin or actin were used as loading controls and the detection was done by using anti-tubulin antibody (Sigma T5168, mouse monoclonal) or anti-actin antibody (ab14128 ...
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bioRxiv - Plant Biology 2020Quote: ... Detection was carried out by chemiluminescence assays after incubation with an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (Sigma or Santa Cruz).
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bioRxiv - Microbiology 2020Quote: ... Simultaneous determination of nitrate and nitrite concentrations induced by the bacteria was done by Vanadium assay with the reduction of nitrate to nitrites by Vanadium (III) combined with detection by the acidic Griess reaction (Sigma-Aldrich, USA), as reported in Miranda et al. ...
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bioRxiv - Microbiology 2020Quote: ... which was un-exposed European pooled serum.23 Secondary detection was with Human IgG (H&L) Antibody Peroxidase Conjugated (Sigma – Aldrich, Germany), developed with Pierce™ TMB Substrate Kit (Thermo Fischer ...
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bioRxiv - Cell Biology 2021Quote: ... Between 22,109 and 26,665 FACS-sorted β-cells were then lysed in Laemmli buffer and loaded on tricine gels for detection of insulin (Sigma, I2018), SNAP tag (NEB ...
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bioRxiv - Genetics 2022Quote: ... Incubation with appropriate secondary HRP-labelled antibody (less than 1h) was followed by detection with Immobilon western chemiluminescent HRP substrate (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Immunology 2022Quote: Formalin-fixed and paraffin-embedded lung sections from mock and IAV-infected mice were prepared as previously described (47) and probed for epithelial or endothelial cells detection using monoclonal mouse anti-αSMA (1:10,000; A2547; Sigma-Aldrich, Darmstadt, Germany), rabbit anti-mouse CD31 (1:50 ...
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bioRxiv - Microbiology 2020Quote: ... A was 1 mM ammonium formate pH 9 (for lincomycin detection, prepared by titration of formic acid 98–100%, Merck, Germany with ammonium hydroxide 28–30%, Sigma-Aldrich, Germany) or A was 0.1% formic acid (for celesticetin detection ...
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bioRxiv - Immunology 2020Quote: ... Detection of GFP and Iba1 required high-temperature antigen unmasking in 0.01 M citrate buffer (pH 6.0) (Sigma-Aldrich, San Diego, CA). Sections were treated with biotin and streptavidin blocking solutions (Vector Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... After further washes with TBS-T, detection was performed using EZ-ECL Chemiluminescence Substrate (Biological Industries, Israel) or Luminata Forte Western HRP Substrate (Millipore Sigma, MA). Membranes were imaged on Biospectrum 500 (UVP ...
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bioRxiv - Cell Biology 2020Quote: ... Duolink® In Situ PLA Probe Anti-Rabbit MINUS and Anti-Mouse PLUS and Duolink® In Situ Detection Reagents (Sigma-Aldrich) were used ...
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bioRxiv - Immunology 2023Quote: ... followed by the addition of anti-human horseradish peroxidase (HRP)-conjugated secondary antibodies for IgG detection (diluted 1:50000, Sigma-Aldrich, A0170) to each well for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... This was followed by flow cytometric detection after staining of bead-bound sEVs with fluorescent anti-CD63 phycoerythrin (PE) (Sigma-Aldrich, USA).
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated for 30min at 37°C in the ligation reaction mix from the Duolink® In Situ Detection Reagents Red (DUO92008, Sigma-Aldrich). The cells were washed again twice in wash buffer A for 5min and incubated for 1h40 at 37°C under slow rotation for the amplification step ...
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bioRxiv - Cell Biology 2023Quote: ... Ligation and amplification were performed according to the manufacturer’s protocol using DuoLink In Situ Detection Reagents Brightfield (Sigma-Aldrich, catalog no. DUO92012). After the PLA reaction ...
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bioRxiv - Microbiology 2022Quote: ... and oligo(dT) primers (Eurofins Genomics) for detection of mRNA or a fluA uni12 forward primer [92] (Sigma-Aldrich, 5’-AGCAAAAGCAGG-3’) to detect vRNA according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The proximity Ligation Assay (PLA) was then performed using the Duolink® In Situ Detection Red as recommended by the manufacturer (Sigma Aldrich). After the PLA reaction was performed ...
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bioRxiv - Cancer Biology 2023Quote: ... blots were visualized by chemiluminescence detection using chemiluminescent horseradish peroxidase reagents (Thermo Fisher, Pierce, cat# 32106 or EMD Millipore, Immobilon, cat# WBKLS0050) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were collected for the detection of cytokines using the Milliplex MAP Mouse Cytokine/Chemokine premixed 32 magnetic bead panel (Millipore, Burlington, MA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were run on a 12% SDS-PAGE gel and transferred to a nitrocellulose membrane for immunoblotting with a MAR detection reagent (Millipore Sigma, MABE1076).
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bioRxiv - Cell Biology 2020Quote: ... Veeco Probes) with a spring constant of 60 pN/nm were functionalized by N1-(3-trimethoxysilylpropyl)diethylenetriamine (30 min, RT, Sigma Aldrich, 06666) followed by heating (30 min ...
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bioRxiv - Cell Biology 2020Quote: ... BSA was coupled to the PEG (3000) for 1h at RT with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 100 mM, Sigma Aldrich, 39391) and N-hydroxy-succinimide (NHS ...
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bioRxiv - Cell Biology 2019Quote: ... were treated for 20 min at room temperature (RT) with 200 µl of Bind-silane solution—a mixture of 714 µl 3-(Trimethoxysilyl)propyl methacrylate (3-TMP, Sigma-Aldrich, M6514), 714 µl of acetic acid in 10 ml of 96% ethanol ...
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bioRxiv - Cell Biology 2019Quote: ... cells were treated with 4N HCl at RT for 30 min and incubated with mouse anti-BrdU at 1:100 (B2531, Sigma, Milan, Italy) in blocking solution (0.1% BSA in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... washed once with 5 mL ice-cold NETN250 buffer and lysed for 15 min at room temperature (RT) in ice-cold NETN250 buffer supplemented 5 mM MgCl2 and 125 U/mL benzonase (Novagen 71206-3). After addition of EDTA and EGTA to respective final concentrations of 5 mM and 2 mM to inactivate the benzonase and centrifugation for 30 min at 16,100 × g ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 1 hour at RT with the following antibodies diluted in blocking buffer: fibronectin (Sigma-Aldrich, St. Louis, MO) and α-smooth muscle actin (α-SMA ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3x PBS washes for 5 minutes at RT before blocking with blocking buffer (BB; 5% normal donkey serum Sigma-Aldrich #S30-M, 3% BSA (Sigma #A4503) and 0.25% Triton-X100 (Sigma #T9284 ...
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bioRxiv - Cell Biology 2021Quote: ... RPE organoids were fixed in 4 % paraformaldehyde (AppliChem GmbH, Darmstadt, Germany) for 20 min at room temperature (RT) and subsequently equilibrated to 30 % sucrose (Sigma-Aldrich, USA) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by 1 h incubation at RT in secondary antibodies (Anti-Rabbit: A0545; Anti-Mouse: A6782, Sigma-Aldrich Quimica S.A., Madrid, Spain) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated for 1 h at RT followed by nuclear counterstaining with DAPI (1 μg/ml in 1 x PBS; Sigma-Aldrich, Germany) for 10 min at RT ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... and 0.4% Triton X-100 in Tris-bufered saline (TBS) at room temperature (RT) for 1 h and then incubated with primary antibodies against Sftpc (AB3786, Millipore; 1:500), Pdpn (8.1.1 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed five times with PBS and incubated 2 h at RT in the dark with FITC-coupled α-rabbit antibodies (1:200 in BSA-PBS, Sigma Aldrich). After washing and drying ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidified chamber at RT for 1 hr with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidity chamber at RT for 1 hour with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were rinsed once with PBS and fixed for 15 min at RT with 4% PFA (Sigma−Aldrich, cat. no. 158127) in 0.1 M phosphate buffer ...