Labshake search
Citations for Millipore Sigma :
2401 - 2450 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... coli DH10B were grown in Luria-Bertani (LB) medium (Sigma, #L3022) at 37 °C ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein fragments were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen) cells ...
-
bioRxiv - Immunology 2024Quote: ... Cells were stimulated with RPMI or Escherichia coli LPS (Sigma-Aldrich) at a concentration of 10 ng/mL for 4h-incubation at 37°C ...
-
bioRxiv - Biochemistry 2024Quote: aSyn was recombinantly produced in BL21(DE3) Escherichia coli cells (Novagen) as previously described 47 ...
-
bioRxiv - Microbiology 2024Quote: ... coli BL21(DE3) was used as an expression system (Novagen #70777) and maintained according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... coli RS218 was stained with Fluorescein isothiocyanate (FITC, #F7250, Sigma-Aldrich). Briefly ...
-
bioRxiv - Molecular Biology 2024Quote: ... IFITs were expressed in Rosetta 2 (DE3)pLysS Escherichia coli (Novagen). Cells were grown to an OD600 of approximately 0.6 in LB media at 37 °C ...
-
bioRxiv - Microbiology 2024Quote: ... 10 ng/mL of LPS (Escherichia coli O55:B5, Sigma-Aldrich) or with supernatants of S ...
-
bioRxiv - Biophysics 2019Quote: ... The DNA encoding AtGLR3.2 residues Ser420-V572 (S1) and P682-N811 (S2) was amplified using gene-specific primers and subcloned into the pET22b vector (Novagen) between NcoI and XhoI sites with a GT linker between S1 and S2 (Armstrong and Gouaux ...
-
bioRxiv - Cell Biology 2020Quote: ... the medium was changed to S1 medium consisting of basal medium supplemented with 6 μM CHIR99021 (Sigma, Cat No. SML1046). Basal medium was prepared by adding 1x GlutaMax supplement (ThermoFisher ...
-
bioRxiv - Microbiology 2020Quote: ... 5A and S1) were carried out on 0.8% w/v LB Agarose supplemented with 1 µg/mL propidium iodide (PI; Sigma-Aldrich). Colony monolayer assays (Figure 3 ...
-
bioRxiv - Systems Biology 2020Quote: ... and His6-tagged GFP gene (primers A18 and 19; Table S1) were separately cloned using InFusion (Clonetech) methods into NdeI/BamHI-linearised pET19b (Novagen).
-
bioRxiv - Immunology 2021Quote: ... Cells were incubated with anti-human antibodies (see Table S1 for additional details) diluted in FACS buffer (DPBS supplemented with 1% FBS and 0.05% sodium azide (Sigma-Aldrich). Samples were analysed using a BD FACSCanto II Flow Cytometer (BD Biosciences) ...
-
bioRxiv - Biochemistry 2021Quote: ... bacteriovorus HD100 genomic DNA using primers detailed in Table S1 Amplified construct DNA was inserted into a modified pET41c plasmid (Novagen, GST-tag removed and thrombin cleavable 8xHis-tag introduced at C-terminus ...
-
bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected with plasmids encoding different ACE2 orthologs (Table S1) by polyetherimide (PEI) (Sigma, St Louis, MO, USA). After 40 hrs incubation ...
-
bioRxiv - Cancer Biology 2021Quote: ... 500 ng CRISPR-Cas9 (PX458) plasmid having the right sgRNA insert (Table S1) was transfected along with transfection reagent Lipofectamine 2000 (Sigma) at a 1:3 DNA to reagent ratio ...
-
bioRxiv - Microbiology 2020Quote: ... was cloned as 10× His-MBP-SUMO-NUP153 (or NSP1)-SNAP constructs (Figure S1) into a pET-28a-derived vector (Novagen) and expressed in BL21-Gold (DE3) ...
-
bioRxiv - Molecular Biology 2020Quote: The phosphorylation of the S1/S2 linker constructs was examined in vitro using purified cyclin B-Cdk1 (Millipore 14-450) and PKA (murine cAMP-dependent protein kinase) ...
-
bioRxiv - Molecular Biology 2024Quote: cDNAs of BTB domains were PCR-amplified using corresponding primers (Supplementary Table S1) and cloned into modified pET32a(+) vector (Novagen) encoding TEV protease cleavage site after 6xHis-tag and Thioredoxin ...
-
bioRxiv - Bioengineering 2023Quote: ... were mixed with ssDNA output oligonucleotides of interest at 2x excess (Table S1) in 1x annealing buffer (5 mM Tris, 1 mM EDTA, 5 mM MgCl, Millipore, cat# 648311-1KG ...
-
bioRxiv - Immunology 2020Quote: ... the SARS-CoV-2-RBD-binding phages were eluted with 100 mM triethylamine (Sigma-Aldrich, Xuhui, Shanghai). All eluted phages were collected and used to infect TG1 cells ...
-
bioRxiv - Immunology 2021Quote: ... Eluted SARS-CoV-2 S containing fractions were concentrated using Amicon Ultra (cut-off: 30 KDa) (Millipore) and further purified by size-exclusion chromatography (SEC ...
-
bioRxiv - Immunology 2020Quote: ... rabbit anti-SARS-CoV S polyclonal sera (BEI Resources) and mouse anti-2xStrep-tag antibody (Sigma-Aldrich). Anti-mouse IgG AF555 ...
-
bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
-
bioRxiv - Genetics 2021Quote: ... Mix-n-Stain CF 555 Antibody Labeling Kit and Mix-n-Stain CF 647 Antibody Labeling Kit (Sigma-Aldrich, MX555S100 and MX647S100) were used according to the manufacturer’s protocol.
-
bioRxiv - Physiology 2020Quote: ... USA) were pulled to a fine tip and silanized in an atmosphere of N,N-dimethyltrimethylsilylamine (Sigma Aldrich, St. Louis, MO, USA). Silanized glass microelectrodes were then back-filled with 100 mmol L-1 KCl and front-filled with K+ ionophore (K+ ionophore I ...
-
bioRxiv - Molecular Biology 2019Quote: ... dissolved in 100 mL PBS containing Ca2+/Mg2+ and 25 mM N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES; Sigma, Vienna, Austria), resulting in a final collagenase concentration of 0.1 U/mL ...
-
bioRxiv - Biophysics 2022Quote: The dyes used in this study and the working dilutions were: 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine (LAURDAN, 4.5 μM, Sigma-Aldrich, Merck, #40227), Hoechst 33342 (80 uM ...
-
bioRxiv - Neuroscience 2019Quote: ... Specimens for histamine immunohistochemistry were prefixed overnight at 4°C in 4% N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDAC, Sigma–Aldrich E6383). Brains were washed six times for 15 minutes each in PBS and subsequently immersed in Poly-L-Lysin (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2023Quote: ... Identification of the VOCs was based on a combination of computer matching with mass spectral libraries (NIST 14) and retention index values derived from an n- alkane solution (c5 to n-c18) (Sigma Aldrich Co.). Only compounds with match factor scores ≥ 70 and signal-to-noise ratios ≥ 10 were selected for quantitation ...
-
bioRxiv - Bioengineering 2024Quote: ... they were simultaneously perfused and soaked for either 48h (n=10) or 72h (n=10) with 1% SDS (Sigma-Aldrich®, USA). All GMs were then agitated with distilled water for 15 minutes ...
-
bioRxiv - Plant Biology 2023Quote: ... followed by a 30-min treatment with 70 µL MSTFA [N-methyl-N(trimethylsilyl)-trifluoroacetamide (69479, Sigma-Aldrich, St. Luis, MO, USA) at 37°C and centrifugation ...
-
bioRxiv - Cancer Biology 2023Quote: Tumors excised from AKB6 xenografts that were treated with vehicle (n=2) and MRTX1133 (n=2) were digested using Liberase (Sigma Cat # 05401020001) to get single cells and sent to RPCI’s Genomics Shared Resources for sequencing using 10X Chromium and Illumina’s NovaSeq instruments ...
-
bioRxiv - Microbiology 2023Quote: ... double-stained cells were also stained with 10 μM Laurdan (6-dodecanoyl-N,N-dimethyl-2-naphthylamine, Sigma Aldrich, Zwijndrecht, the Netherland). Laurdan was excited at 395 nm and 470 nm emission wavelength was used for rigid membrane and 508 nm for fluid membrane environment ...
-
bioRxiv - Microbiology 2023Quote: ... containing 1 mg/ml X-gal (Sigma-Aldrich; prepared fresh as a 40 mg/ml stock in N,N-Dimethylformamide (Sigma-Aldrich)) overnight at 4 °C ...
-
bioRxiv - Developmental Biology 2024Quote: ... 90 µL of N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-Butyldimethyl-chlorosilane (TBDMS; Cat. No. 375934, Sigma-Aldrich; St Louis, MO.) was added and samples were incubated under shaking at 37°C for 30 minutes ...
-
ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... 90 µL of N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-Butyldimethyl-chlorosilane (TBDMS; Cat. No. 375934, Sigma-Aldrich; St Louis, MO) was added ...
-
bioRxiv - Biophysics 2021Quote: ... 0.3% 10 mg/ml N-hydroxysuccinimide (NHS, Sigma Aldrich) in dimethyl sulfoxide (DMSO ...
-
bioRxiv - Biophysics 2021Quote: ... 0.3% 10 mg/ml N-hydroxysuccinimide (NHS, Sigma Aldrich) in dimethyl sulfoxide (DMSO ...
-
bioRxiv - Cell Biology 2020Quote: ... (cat n. R0883, Sigma-Aldrich, St. Louis, MO, USA), supplemented with 10% heat-inactivated FBS ...
-
bioRxiv - Cell Biology 2020Quote: For the combined N-acetylcysteine (NAC, Sigma-Aldrich, #A7250) and formaldehyde (FA ...
-
bioRxiv - Cell Biology 2019Quote: ... N methyl-bis-acrylamide cross-linker (Sigma-Aldrich, M1533) in PBS (Table 1) ...
-
bioRxiv - Microbiology 2021Quote: ... and 100µL of 0.25 N Folin reagent (Sigma-Aldrich) was added to each tube ...
-
bioRxiv - Developmental Biology 2021Quote: ... embryos develop in N-Phenylthiourea (PTU, Sigma-Aldrich, P7629) in Volvic source water (0.003% final) ...
-
bioRxiv - Physiology 2022Quote: Liver triglycerides were measured using triglyceride determination kit from Sigma (catalog n°TR0100, Sigma-Aldrich, Saint-Louis, MO, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... Capsaicin (8-Methyl-N-vanillyl-trans-6-nonenamide, Sigma), RQ00203078 (Tocris ...
-
bioRxiv - Genomics 2020Quote: ... 0.3% Triton X-100 (Sigma Aldrich, Cat. N: X100). Staining was performed overnight at 4°C with anti-Pax6-A488 (1:200 ...
-
bioRxiv - Molecular Biology 2019Quote: ... and 20 mM deSUMOylation inhibitor N-Ethylmaleimide (Sigma, E3876). For Piwi/Arx/Panx coIP with Su(var)2-10 the lysis buffer contained 0.4% NP40 ...
-
bioRxiv - Biochemistry 2019Quote: N-Acetyl-D-mannosamine (Sigma-Aldrich, cat. no. A8176)
-
bioRxiv - Biochemistry 2019Quote: N-Acetyl-D-galactosamine (Sigma-Aldrich, cat. no. A2795)