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Citations for Millipore Sigma :
2501 - 2550 of 2557 citations for PSMD6 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and in vitro cultured for 18-24 hours in media supplemented with or without peptide at 0.5 µM (or, as positive control, with 1 µg/ml Staphylococcal enterotoxin B (SEB, Sigma Aldrich, St. Louis, USA)) ...
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bioRxiv - Molecular Biology 2021Quote: ... Bead-particle complexes were washed once with 200 µl washing buffer (20 mM Tris-HCl pH 7.5 and 150 mM NaCl) and subsequently eluted with FLAG peptide (30 min at 37 °C; 200 μg/ml in washing buffer, Sigma Cat. No. F3290) and lysed by addition of Amphipol A8–35 (Anatrace ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...
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bioRxiv - Cell Biology 2021Quote: The peptide/ZO1 interaction was detected in situ using the Duolink secondary antibodies and detection kit (Sigma, St Louis, MO, Cat: 92002, 92004) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and an antibody against calcitonin gene-related peptide (CGRP; polyclonal rabbit anti-rat, 1:5000; #C8198, AB_259091; Sigma-Aldrich, St. Louis, MO, USA) or growth associated protein-43 (GAP43 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were eluted from Flag magnetic beads through adding 5 package of beads gel volume of 150 ng/μl 3 x Flag peptide (Sigma-Aldrich, cat # 4799) in 1 × TBS buffer and incubating in a rotator for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the Anti-Flag M2 Affinity Gel was incubated with 2x vol of 100 µg/ml Flag-Peptide (in TBS, Sigma-Aldrich, cat.no. F3290) at 4 °C and 350 rpm agitation for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were washed two times with 1 mL Pierce Lysis Buffer using a magnetic rack and eluted in 100 μl Pierce lysis buffer containing 3X FLAG® Peptide (Sigma, Cat. # F4799) at 20 μM ...
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bioRxiv - Neuroscience 2024Quote: ... QuickCIP was heat inactivated at 80 °C for 2 min and the peptide solutions were desalted using C18 StageTips (Empore C18 SPE Disks, Sigma Aldrich, Darmstadt, Germany). Briefly ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from the spleen and lymph nodes of OT-II Cas9 double-transgenic mice or Cas9 transgenic mice and activated with splenocytes irradiated at 30Gy and OVA peptide (10mg/mL) (Sigma Aldrich O1641-5mg) for OT-II lung inflammation model ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with Li-COR Odyssey Blocking Buffer and then incubated with primary antibodies: anti-FLAG (Sigma F1804, 1:1000 for proSAAS and 7B2), anti-TDP-43 (Proteintech 10782-2-AP ...
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bioRxiv - Microbiology 2020Quote: ... incubated with the anti-PnpA quail antibody (1:10000 dilution) in blocking buffer followed by incubation with an anti-chicken alkaline phosphatase conjugated secondary antibody (Sigma, Ref A9171, 1:10000 dilution) and NBT/BCIP development.
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bioRxiv - Cell Biology 2021Quote: ... the cells were blocked with the blocking buffer (3% bovine serum albumin [BSA, Sigma-Aldrich A3059] and 0.1% saponin [Sigma-Aldrich S4521] dissolved in DPBS) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Unspecific antibody binding was prevented by incubating the blots in blocking solution containing 5% non-fat milk or 5% BSA (Sigma-Aldrich, St. Louis, MO, USA) in 1x Tris-buffered saline with 0.1 % Tween 20 (TBST ...
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bioRxiv - Genetics 2021Quote: ... followed by blocking with milk or bovine serum albumin (BSA) and antibody labelling with specific antibodies to FLAG (Sigma F1804; 1:5000 in 3 % milk) or RPS-6 (Abcam ab70227 ...
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bioRxiv - Bioengineering 2023Quote: ... followed by blocking the non-specific binding sites with PBS supplemented with 1%v/v bovine serum albumin (BSA) (Sigma Aldrich, St. Luis, MO, USA). The cells were then incubated with primary antibody rabbit anti-YAP1 (1 ...
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bioRxiv - Neuroscience 2024Quote: ... Blocking solution was applied for 30 min at room temperature and consisted of 10% fetal bovine serum (Honduras Origin, Sigma Aldrich, St. Louis, MO, USA) and 0.1% Saponin (Sigma Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... the ORF of CLEL6 lacking the predicted signal peptide was amplified by PCR from a previous construct and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). Correct orientation and translational fusion with the C–terminal His-tag was verified by sequencing ...
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bioRxiv - Immunology 2021Quote: ... 1 mM penicillin-streptomycin and 55 μM β-mercaptoethanol) containing 10 nM either OVA257-264 (H2N-SIINFEKL-OH) or YopE69-77 peptide and brefeldin A (BFA, Sigma, 5 μg/ml) for 4.5 h at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... The beads were washed tree times with TBS solution and FLAG-HDA6 was eluted with 200 ng/µl of Flag-Peptide (Sigma-Aldrich, Cat No F3290) by incubation the resin with synthetic peptide rotating for 30 min at 4 °C.
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bioRxiv - Plant Biology 2020Quote: ... ORFs of PSK1 and RGF1 lacking the predicted signal peptides were amplified by PCR from cDNA and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). C-terminal His tags were added by including 6 His codons in the reverse PCR primers ...
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bioRxiv - Microbiology 2019Quote: ... and added to wells in a sterile 96-well microtiter plate containing two-fold dilutions of each peptide with 0.01% v/v acetic acid (Sigma Aldrich, St Louis, MO, USA) and 0.2% w/v bovine serum albumin (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... and immunoprecipitated overnight at 4 °C with 2 µg affinity-purified rabbit anti-PKM2 antibody raised against a C-terminal peptide (Sigma cat. # SAB4200105, Lot #030M4874) pre-coupled to Dynabeads sheep M-280 anti-rabbit IgG beads (LifeTech) ...
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bioRxiv - Molecular Biology 2020Quote: ... The beads were subsequently washed another three times with 1 ml FLAG wash buffer II and eluted in 100 μl FLAG elution buffer (FLAG wash buffer II with 0.5 mg/ml Flag peptides (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The purified BGLF4 was then used for kinase inhibition assays using the C-terminal fragment peptide of retinoblastoma protein (RB) as substrate (Millipore Sigma cta# 12-439). The remaining assay parameters were the same as those applied for PknB except for the following changes ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit affinity isolated anti-actin 1:3,500 diluted in PBST (Cat# A2066, RRID: AB_476693, immunogen: synthetic peptide corresponding to the C-terminal actin fragment SGPSIVHRKCF, Sigma-Aldrich, St. Louis, MO, USA), and rabbit polyclonal anti-AA-NAT 1:15,000 diluted in PBST (AB3314 ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were immunized on day 0 by subcutaneous injection in the flank region with a total of 200 μl of the antigen PLP139-151 peptide (200 μg/mouse) emulsified in Complete Freund’s Adjuvant CFA (Sigma Chemicals, St. Louis, MO, USA) supplemented with 4 mg/ml heat-killed Mycobacterium tuberculosis H37Ra (400 μg ...
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bioRxiv - Bioengineering 2024Quote: ... The molecular weight of the synthesized peptides was confirmed using MALDI-TOF Mass (MALDI-TOFMS AXMA ASSURANCE™ [Shimadzu]) with α-cyano-4-hydroxycinnamic acid matrix™ (Sigma-Aldrich). Synthesized peptide sequences for ELISA are shown in Table S4.
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bioRxiv - Neuroscience 2021Quote: ... and immunoblotted overnight at 4°C with shaking in Intercept Blocking Buffer with the following antibodies: α GluA2 (1 µg/mL; Millipore, MAB397 or Alomone, AGC-005); α GluA1 (1 µg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... after which the slices were washed with the blocking solution 3 times and stained with the nuclear marker DAPI (Sigma–Aldrich, 1 mg/ml 1:1000) for 15 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... at a concentration of 1:500 was used to detect myc tagged ATR and incubated at room temperature for 1 h in blocking buffer (1 % BSA [Sigma], 0.2 % Tween-20 in 1x PBS) in a wet chamber ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by 1-hour incubation at RT with the diluted secondary antibody after which the slices were washed with the blocking solution 3 times and stained with the nuclear marker DAPI (Sigma–Aldrich, 1 mg/ml 1:1000) for 15 min at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies used in this study were: anti-acetylated α-tubulin (Antibody registry ID: AB_477585, clone 6-11B-1, mouse IgG2b, Sigma, diluted 1:250 in blocking solution), anti-SV2 (AB_2315387 ...
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bioRxiv - Neuroscience 2021Quote: ... Nitrocellulose membranes were incubated in Odyssey blocking solution from Licor (30 min at RT) and later blotted with primary mouse anti-Htt MAB5492 from Millipore (12–16 h at 4 °C). Membranes were washed 3 times in PBS-Tween 0.1% and then incubated with secondary Alexa680-conjugated goat anti-mouse (Licor ...
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bioRxiv - Microbiology 2020Quote: ... an independent negative control was included by surface blocking: measurements were obtained before and after injection of a JAM-A antibody (Sigma Aldrich, concentration of 10 μg/ml) to the sample surface to block JAM-A binding ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was cut at 25 kDa to separate actin and LC3 bands and primary antibody in 5% BSA blocking buffer (anti-LC3B, rabbit, Sigma-Aldrich L7543 at 1:1000 and anti-actin, mouse, Sigma-Aldrich A2228 at 1:2000) was incubated overnight at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Overnight incubation with primary antibodies diluted in blocking/permeabilisation buffer (GFP – 1:1000 Ab13970 Abcam, TH – 1:1000 Ab152 Sigma, IL33 – 1:50 AF3626 R&D) were performed overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Permeabilization was performed in 3% hydrogen peroxide solution (hydrogen peroxide Fisher chemical code H/1750/15) and incubated in 1% BSA blocking buffer (BSA Sigma Aldrich A2153-100G, 1003353538 source SLBX0288). A multiplex panel of antibodies against CD4 (Rabbit ...
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bioRxiv - Molecular Biology 2024Quote: ... midguts were blocked and permeabilised overnight at 4°C in midgut blocking buffer (0.25% [v/v] Triton X-100, 5% [w/v] BSA [Sigma-Aldrich, Cat no. A4503-50G] in dPBS). Midguts were incubated with primary antibodies (apicoplast marker acyl carrier protein [ACP] anti-serum 79 at 1:250 ...
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bioRxiv - Systems Biology 2021Quote: ... The biotinylated MERS-E peptide was FITC labeled (fMERS: biotin-Ahx-DSK(-fluorescein)PPLPPDEWV) as follows: the peptide was mixed with sub-stoichiometric FITC (Sigma-Aldrich, St. Louis, MO, USA) in a basic HEPES buffer (pH 8.2) ...
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bioRxiv - Molecular Biology 2022Quote: ... The bound proteins were eluted by Flag elution buffer (50 mM NaPi [pH7.5], 10% glycerol, 30 mM NaCl, 200 µg/ml 3xFlag peptide [SIGMA], 0.1 mM PMSF and PI tablet) (Uno et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Polyclonal CatSperτ antibodies were generated by immunizing rabbits with KLH carrier-conjugated peptides corresponding to 359th – 377th (EKLREKPRERLERMKEEYK, α-τ-359; Open Biosystems) or 482nd – 500th (QIVEENEMPHLPKTSEPED, α-τ-482; Sigma Aldrich) residues of CatSperτ ...
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bioRxiv - Immunology 2023Quote: ... each comprising of 53 15-mer peptides overlapping by 11 residues, were purchased from Mimotopes Pte Ltd (Clayton, Victoria, Australia) and reconstituted in DMSO (Sigma-Aldrich, St. Louis, Missouri, USA). Plasmids encoding ancestral and variant SARS-CoV-2 spike protein were purchased from Addgene (170442 ...
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bioRxiv - Cell Biology 2020Quote: ... were subjected to immunoblot analysis with specific primary antibodies (Table 1) and secondary antibodies (anti-mouse/rabbit IgG coupled to horseradish peroxidase; Sigma Aldrich; 1:5000 dilution in blocking buffer). Detection of chemi-luminescent signal was performed with Clarity ECL Western Blotting substrate (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were incubated during 48 h at 4°C in a blocking solution containing the primary antibody against TH (rabbit anti-TH, Millipore AB152, lot 2795024 and 3114503 (1:1500) RRID ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were dissected out and washed extensively in PBS before blocking in staining buffer (10% normal goat serum, Sigma-Aldrich, and 2% Triton X-100 in PBS) for half an hour ...
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bioRxiv - Cell Biology 2019Quote: ... the slides with attached intact axonemes were treated overnight at 4°C with blocking buffer containing the diluted primary antibody (mouse anti-acetylated tubulin, Sigma-Aldrich, clone 6-11B-1, 1:1000). On the next day ...
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bioRxiv - Cancer Biology 2020Quote: ... The equivalent of 5 mg of protein was further enriched for phosphorylated tyrosine peptides by overnight immunoprecipitation (pY-IP) using a cocktail of anti-phosphotyrosine antibodies (4G10, Millipore; PT-66, Sigma;p-Tyr-100, Cell Signaling Technology).