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Citations for Millipore Sigma :
2451 - 2500 of 2557 citations for PSMD6 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and proteins were eluted with 4 mL of the same buffer but containing 100 μL (5 mg mL−1) Flag peptide (Sigma-Aldrich F4799). The eluate was spin concentrated (Sigma-Aldrich CLS431485-251A ...
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bioRxiv - Neuroscience 2021Quote: ... This antiserum detects human α-CGRP and β-CGRP but does not cross-react with any other peptide (data supplied by Sigma). We used a rabbit polyclonal antibody (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed 3 times with 500 µL of lysis buffer and bound protein was eluted with 50 µl of elution buffer (100 µg/mL FLAG peptide (Sigma-Aldrich #F3290), 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged proteins were eluted by addition of 40 µL IP buffer supplemented with 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) and incubated for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... the complex was collected in buffer containing 0.2 mg/ml FLAG peptide and concentrated to 400 μL in an Amicon® Ultra-15 Centrifugal Filter Unit (Millipore, Burlington, MA). Complexes were loaded onto a Superose 6 Increase 10/300GL column (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... Peptide samples were loaded onto a Sigma-Aldrich Ascentis Peptides ES-C18 column (2.1 mm x 100 mm, 2.7 µm particle size; Sigma-Aldrich, St. Louis, MO) via an Infinity Autosampler (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 individual peptides (2 μg/ml) or blank control (complete medium with DMSO) onto sterile nitrocellulose MSIP 96-well plates (Millipore, Bedford, MA) coated with capture antibodies against mouse IFN-γ ...
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bioRxiv - Cancer Biology 2023Quote: ... beads washed five times with pre-chilled NET resuspension buffer and eluted under native conditions by competition using 3x FLAG peptide (Millipore Sigma, F4799). Samples were boiled in the Laemmli SDS sample buffer (Thermofisher ...
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bioRxiv - Immunology 2023Quote: ... were cocultured for 6 hours with autologous herpesvirus papio-immortalized B lymphoblastoid cell lines (B-LCLs) pulsed with Nef peptides (Sigma-Aldrich Japan) at 1 μM concentration or as indicated otherwise under GolgiStop (monensin ...
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bioRxiv - Cancer Biology 2023Quote: ... and seeded at 1 x 105 in a 24 well plate and then pulsed with 1ng/mL OVA peptide (Sigma, #S7951-01mg) for 3 hours ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated in the presence of either 1 μg/mL OVA peptide (323-339; pOVA) (Sigma-Alrdrich; St Louis, MO, USA), 2.5 μg/mL concanavalin A (ConA ...
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bioRxiv - Immunology 2023Quote: ... 2.5 x 105 splenocytes from OT-1 mice were seeded in one ml of complete media in 24 well plates and 0.5µg/ml of peptide (Sigma-Aldrich, cat. number S7951). After 48 hours blasts were expanded with 100 U/ml of rmIL-2 in fresh media and harvested on day four or six after initial stimulation.
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bioRxiv - Biochemistry 2024Quote: ... the gel was washed with 10 column volumes of lysis buffer and subsequently eluted with 100 µg/mL free Flag peptide (Sigma Aldrich, F3290) in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 mins and incubated in blocking PBS solution containing 2% BSA (AppliChem, 9048-46-8) and 0.2% Triton™ X-100 (Sigma-Aldrich, 9002-93-1) for 30 mins to permeabilize cell membrane and block unspecific antigen binding ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody was incubated at room temperature protected from light for 1 hour in Intercept®(TBS) Protein-Free Blocking Buffer with 0.01% Tween®20 (Sigma, St. Louis, MO) using IRDye® 800CW Goat anti-Mouse IgG secondary antibody at 1:10,000 (LI-COR Lincoln ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibody was incubated at room temperature for 2 hours in Intercept®(TBS) Protein-Free Blocking Buffer with 0.01% Tween®20 (Sigma, St. Louis, MO) using STAT1 1:1000 (#14994 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary and secondary antibody incubations were performed for 1 hour at 25°C in blocking buffer with anti-Flag-M1 (Sigma-Aldrich, #F3040, 1:1000) conjugated with Alexa-647 (Molecular Probes ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane is incubated for 1 h in a blocking buffer of PBST (PBS supplemented with 0.1 v/v % Tween) and 10% (v/w) dry skim milk (Sigma-Aldrich, St. Louis, MO, USA). The membrane is washed three times with PBST for 5 min and then incubated for 1 h at 25 C with a primary anti-dynein antibody (sc-13524 ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in blocking solution with the following primary antibody: 1:1000 mouse anti S-100 β subunit (S2532, Sigma-Aldrich, St. Louis, MO). Sections were washed and then incubated for 2h in blocking solution with the following secondary antibody at a 1:500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... secondary antibodies were applied for 30 min at RT at 1:400 dilution in blocking solution containing 1:10,000 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich, St. Louise, MO, USA) in the closed humid chamber ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were rinsed with the blocking solution and incubated with secondary antibodies for 2 h at room temperature (FITC-conjugated anti-rabbit IgG, AP132F, Millipore, Darmstadt, Germany; 1:200). The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were further incubated at room temperature for 1 h with a blocking buffer (3% normal donkey serum; S30, Chemicon by EMD Millipore, Billerica, MA, USA) in PBS solution containing 0.2% Triton X-100 and 0.05% Tween 20 (PBST) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... prepared in antibody blocking solution containing 0.5% BSA (Fisher, Pittsburgh PA, USA #BP9703-100) and 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA #T9284) for 48-72 hours at 4°C in a shaker ...
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bioRxiv - Neuroscience 2019Quote: ... following by primary antibody incubation overnight at 4°C (rabbit anti-pRIPK3, Abcam; mouse anti-TH, Millipore; mouse anti-pMLKL: Millipore rabbit anti-TH, Millipore in blocking/permeabilizing solution). Sections were washed in 0.1M TBS and then incubated in secondary antibodies for 2 hours at RT in TBS (goat anti-rabbit Alexa Fluor 488 ...
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bioRxiv - Genomics 2021Quote: ... and nucleolin (D4C7O) rabbit mAb (Cell Signalling cat # 14574) appropriately diluted in Odyssey® Blocking Buffer (TBS) 0.1% TWEEN® 20 (Sigma Aldrich cat # P1379) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... in 50 μL of blocking buffer were added for 1 hour followed by 50 μL ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5000 dilution) in blocking buffer for 1 hour at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: ... and then blocked for 1 hour in blocking buffer (1% BSA and 10% normal donkey serum, 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA). Primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... the calcium free medium was replaced with the cell culture medium with or without E-cadherin blocking monoclonal antibody DECMA-1 (Millipore, 10 µg mL−1), and cells migration was carried out for 4 hours at 37°C in a 5% CO2 humidified incubator.
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bioRxiv - Genetics 2022Quote: ... Membranes were washed three times with TBST and incubated with blocking buffer containing Rabbit IgG HRP Linked Whole Ab (Sigma cat no. GENA934-1ML) at 1:5000 for two hours with gentle mixing ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in primary antibodies (diluted in the blocking solution, see Table 1) against myc (rabbit c-Myc, 1:1000, Sigma-Aldrich, C3956, RRID: AB_439680) and FLAG (mouse-FLAG ...
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bioRxiv - Cell Biology 2024Quote: ... Permeabilization buffer was removed and washed 3×2 minutes before blocking for 1 hour at room temperature in a 1% Bovine Serum Albumin (BSA) (Millipore Sigma, cat: 2910-OP) in DPBS solution ...
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bioRxiv - Developmental Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies in blocking solution: rabbit anti-GFP (MBL Cat# 598; 1/300) and mouse anti-HA (Sigma, Cat# 12ca5; 1/50). On the next day ...
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bioRxiv - Genetics 2024Quote: ... by transferring them in a small drop of blocking solution to the surface of a 0.01% poly-L-lysine (Sigma Aldrich, Cat# 25988-63-0) coated slide ...
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bioRxiv - Microbiology 2023Quote: ... After cell permeabilization and blocking with 1% normal goat serum (Nacalai Tesque) in dilution buffer [0.3% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) in PBS containing 3% bovine serum albumin (Nacalai Tesque)] ...
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bioRxiv - Biochemistry 2022Quote: ... Primary antibody were diluted in Blocking Buffer as described below and incubated with samples for 2 hours: γ-H2AX monoclonal mouse antibody JBW301 (Millipore Sigma, 1:1000). Samples were washed with Blocking Buffer twice for 5 minutes each then treated with secondary antibody diluted 1:200 for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Nitrocellulose membranes were blocked for 1 h with the blocking buffer containing 5 % dried milk in TBS-Tween 1 % and incubated over-night with primary antibodies: anti-αTubulin (Sigma, T6074, 1:1000 dilution), anti-acetylated Tubulin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... in 50 μl of blocking buffer were added for 1 h followed by 50 μl ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5.000 dilution) in blocking buffer for 1 h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... iSMCs were incubated for 30 mins in blocking solution containing 2% BSA (AppliChem, 9048-46-8) and 0.5% Triton™ X-100 (Sigma-Aldrich, 9002-93-1). The blocking solution was then discarded and iSMCs were incubated for 2 hrs with primary antibodies diluted in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... After blocking for 1 hour in blocking buffer (for Fig. 1D: 5% Protifar; for Suppl. Fig. 2B: 0.2% v/v Fish skin gelatin (Sigma–Aldrich, St. Louis, MO, USA)+ 0.1% v/v Tween–20 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated in blocking buffer for 1 hr at room temperature followed by incubation with rabbit anti-RBM10 (Sigma-Aldrich HPA034972, 1:2000), rabbit anti-HDAC1 (GeneTex GTX100513 ...
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bioRxiv - Neuroscience 2024Quote: ... and a primary rat antibody against myelin basic protein (MBP) (1:50 in blocking solution; Cat. No. MAB386, Sigma-Aldrich, St. Louis, MO, USA). Following five 5-minute washes at 20°C with PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were subsequently washed five times with ice-cold lysis buffer freshly supplemented with 20 mM NEM and subsequently incubated with Flag peptide (2 mg/mL, Sigma-Aldrich, F-3290) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The immunoprecipitates on protein A agarose or sepharose were either boiled directly in sample buffer or eluted with FLAG peptide (Sigma, 200 μg/mL) or HA peptide (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The selected mouse and rabbit immunoglobulins were affinity purified on protein G SepFast resins (BioToolmics, UK) or on peptide used for immunization and conjugated to a cyanogen bromide-activated-Sepharose® 4B (Sigma-Aldrich, USA), respectively ...
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bioRxiv - Microbiology 2022Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... BG-oligonucleotide-labeled SNAP proteins bound to resin were again washed with wash buffer and eluted using 0.2 mg/mL FLAG peptide (Sigma-Aldrich, St. Louis, MO) overnight at 4°C with rotation ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... Beads were washed 3 times with lysis buffer and TFEB or TFE3 eluted using 200 ng/μL 3X-flag-peptide (Sigma-Aldrich, F4799-4MG).
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bioRxiv - Biochemistry 2020Quote: ... isolated from the BBPHs were added to the reaction mix before adding the enzyme to assess the inhibition potential of the peptides and the results have been compared with a standard ACE inhibitor Lisinopril (L2777, Sigma-Aldrich, MO, USA), used as positive control (50 µL of 2 µg/mL).