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Citations for Millipore Sigma :
2301 - 2350 of 2557 citations for PSMD6 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The fixed thin gel cultures were permeabilized in PBST (0.2% (vol/vol) TritonX-100 in PBS) at room temperature for 1h and blocked with blocking buffer (5% (wt/vol) BSA (Sigma, cat. # A2153), 2% (wt/vol ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were blocked for 1 hour with blocking solution and then incubated with the indicated primary antibody pairs as multiplex with N1/DLL4 (Sigma, Duolink DUO92008), N1ex/JAG1ex (sigma Duolink DUO92013) ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk/TBST for one hour at room temperature and incubated overnight at 4°C with the following primary antibodies diluted in blocking reagent: 1 μg/mL mouse α-alpha-tubulin (DM1A, EMD Millipore), 1 μg/mL rabbit α-LEM-2 (Novus Biologicals) ...
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bioRxiv - Cell Biology 2023Quote: ... Hepatocytes were permeabilized for 1 h at room temperature in blocking buffer consisting of 2% Bovine Serum Albumin (Millipore Sigma cat A2153) and 0.2% Triton X-100 (Millipore Sigma cat 648466 ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were then added and samples incubated overnight at 4°C in blocking solution at the following dilutions: mouse α-FLAG (1:1000, M2 clone, Sigma #F3165), mouse α-V5 (SV5-Pk1 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hr at RT and blotted overnight at 4°C in the same blocking solution with the following antibodies: Rabbit Anti-GluK1 (1:1000; Millipore: 07-258), Rabbit Anti-GluK2/3 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were incubated with 5% BSA in 0.1% TBS-Tween for 1 hr at room temperature for blocking step and incubated with 1:1000 anti-RFC1 antibody produced in rabbit (Sigma-Aldrich; AV44167) at +4°C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... T8787)/PBS at 37C for 2 days and subsequently immersed in a blocking solution of 10% DMSO + 6% donkey serum (EMD Millipore: S30) + 0.2% Triton X-100/PBS at 37C for 3 days ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.6) for 30 min at RT before incubation with blocking solution consisting of 3% bovine serum albumin (Sigma, Merck, Germany, #A9418) and 0.3% Triton X-100 in PBS 1X ...
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bioRxiv - Neuroscience 2023Quote: ... For IHC we permeabilized and blocked sections with blocking solution (PBS containing 5% normal goat serum [Thermo Fisher Scientific # 10000C] and 0.1% Triton X-100 [Millipore-Sigma # X100-100ML]) for 60 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... then in the anti-dopamine receptor antibody diluted in the blocking solution for 5-6 days at 4°C (rabbit anti-dopamine D1A receptor, 1:100, Sigma-Aldrich AB1765P). Sections were washed in PBS and incubated for 2 h at RT in the secondary antibody (goat anti-rabbit Alexa Fluor 555) ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4°C with the corresponding primary antibodies diluted in blocking buffer: mouse anti-β-Actin 1:2000 (Sigma, #A544); mouse anti-3R-Tau 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4°C in blocking solution with the addition of the following antibodies (dependent on experiment): goat anti-ChAT (Millipore Sigma AB144P), rabbit anti-cFos (Synaptic System 226 008) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were permeabilised overnight at 4°C and then transferred to blocking solution (10% DMSO, 6% normal donkey serum (NDS; Sigma-Aldrich, D9663), 0.2% Triton X-100 in DPBS ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was discarded and pelleted nuclei were resuspended in 3 ml blocking buffer (1xPBS [Sigma-Aldrich, P4417-50TAB, US], 1% BSA [Sigma-Aldrich, A2153-10G ...
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bioRxiv - Neuroscience 2024Quote: ... Fixed coverslips were transferred into 12-well plates and outlined with a hydrophobic pen and blocked using 40 μL of Duolink blocking buffer (Sigma Aldrich, DUO82007) at 37 °C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then incubated with PBG blocking buffer (0.2% w/v, cold water fish gelatin-Sigma G-7765; 0.5% w/v, BSA-Sigma A-2153 in PBS) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated in blocking buffer (5% non-fat dry milk (Carlroth, Cat no. T145.1) in TBST containing 0.005% Tween-20 (Sigma, Cat no. P7949-500ML), or 0.02% Tween-20 (TBST-XL)) ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated in antibodies for 48h at 4 °C in a blocking solution and immunolabeled with chicken anti-Nefh (1:5000, EMD Millipore, AB5539), mouse anti-Syn1 (1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... Slides were then incubated in a humidity chamber at room temperature for 1 hour with blocking solution covering the tissue (5% normal donkey serum (Sigma Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Biophysics 2021Quote: A 3 μL droplet of the 500 μM peptide solution was applied to glass slides and stained with a 10 mM Congo Red (Sigma©) solution in PBS (137 mM NaCl ...
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bioRxiv - Microbiology 2019Quote: ... The eluted tryptic peptides were dried by speed-vacuum centrifugation and then desalted through ZipTip C18 Pipette tips (Millipore/Sigma-Aldrich). Samples were reconstituted in 10 µL of 0.1% formic acid before their analysis by nLC–MS/MS ...
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bioRxiv - Microbiology 2019Quote: ... The eluted tryptic peptides were dried by speed-vacuum centrifugation and then desalted through ZipTip C18 Pipette tips (Millipore/Sigma-Aldrich). Samples were reconstituted in 10 µL of 0.1% formic acid before their analysis by nLC–MS/MS ...
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bioRxiv - Developmental Biology 2021Quote: ... The FLAG tagged proteins were then eluted in the FLAG wash buffer containing a final concentration of 500 ng/μl of FLAG peptide (Sigma, F4799).
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bioRxiv - Biochemistry 2020Quote: Peptides were desalted and concentrated with C18 ZipTips (10 μL pipette tip with a 0.6 μL resin bed; Millipore, MA, USA). Samples were dried and reconstituted in 25 or 50 μL 0.1% formic acid depending on the specific analysis ...
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bioRxiv - Immunology 2022Quote: PBMC or expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-2 peptides (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Two elution steps of the FLAG-tagged protein-DNA complexes were performed with 200 μL of FLAG buffer (TBS buffer plus 100 μg/ml of 3XFLAG peptide from Sigma-Aldrich) and the samples were incubated 30 min at RT on a rotating wheel (20 rpm) ...
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bioRxiv - Genetics 2019Quote: ... We used the anti-HA high affinity monoclonal antibody from rat to recognize the HA-peptide (clone 3F10, ref 11 867 423 001, Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Beads were washed 5 times in NP40 buffer and precipitated proteins were eluted by boiling with SDS sample loading buffer or elution with 3X FLAG peptide (Sigma-Aldrich). Whole cell lysates and immunoprecipitated samples were analyzed by western blot.
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bioRxiv - Biochemistry 2019Quote: ... and eluted by competitive elution in Flag Wash Buffer supplemented to 200 ug/mL Flag peptide and 250 ug/mL human insulin as a carrier (Sigma-Aldrich). The protein was then passed over a 2 mL amylose column (NEB ...
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bioRxiv - Immunology 2021Quote: ... were coated with 50 µL/well of 1 ng/µL solution of fragments 308-331 or 421-438 from HIV gp120 peptides or fragment 579-601 of HIV gp41 (Sigma-Aldrich) in carbonate-bicarbonate buffer (pH 9.6 ...
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bioRxiv - Cell Biology 2021Quote: ... Elution of the co-immunoprecipitated complex was performed by gentle shake of the beads at 4°C in elution buffer supplemented with 3X FLAG peptide (Sigma-Aldrich) for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and eluted with FLAG elution buffer [identical to FLAG wash buffer but also containing 3x FLAG peptide (100 µg/ml, Sigma Aldrich)] ...
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bioRxiv - Bioengineering 2020Quote: ... Bound proteins were eluted by competition with four 2 x CV of DPBS containing 100 µg/mL FLAG peptide (Sigma Aldrich). Purified proteins were formulated in DPBS by buffer exchange through desalting columns (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... All experiments were prepared in PBS and contained either 0.2 μM of the respective DegP enzyme (monomer concentration) and 7.5 μM of p23 peptide or 10 μM of the enzyme with 50 μM β-Casein (Sigma-Aldrich) and 100 μM of the Abz-KASPVSLGYNO2D reporter peptide ...
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bioRxiv - Neuroscience 2021Quote: ... Arc-Myc-FLAG was eluted by incubating beads in 50 µL of RIPA buffer containing 25 µg of FLAG peptide (Sigma-Aldrich) for 2 h at 25°C with gentle agitation ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were washed three times with the protein elution buffer (20 mM Tris-HCl, 150 mM NaCl, 10% glycerol) and eluted by adding 3× FLAG peptide (Sigma-Aldrich) in 60 µL of the protein elution buffer following the manufacturer’s instruction.
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bioRxiv - Microbiology 2021Quote: ... The beads were washed 5 times in RIPA buffer and the immunoprecipitated proteins eluted using 150 μg/mL 3x Flag peptide (Sigma-Aldrich) in elution buffer (50 mM Tris/HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... subtilis was achieved by 5 min incubation with small cation specific channel-forming antimicrobial peptide Gramicidin ABC (10 µM; gABC; Sigma Aldrich), and membrane permeabilisation by 5 min incubation with pore-forming lantibiotic Nisin (10 μM ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with increasing concentrations (0 to 120 μg/ml) of a reverse sequence control peptide (Ser-Asp-Gly-Arg-Gly/SDGRG, Sigma-Aldrich) and an RGD peptide (Gly-Arg-Gly-Asp-Ser/GRGDS ...
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bioRxiv - Molecular Biology 2022Quote: ... Each recombinant protein was eluted twice in 250 µl of EB containing 1 mM DTT and 250 µg/ml 3xFLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... in-house identifier: AfR364-3P2) and hCAP- D3 pS1474 peptide (C-QQWNVRpSPARNK; in-house identifier: AfR358-3P2) were generated commercially (SIGMA Genosys). Secondary antibodies used in the current study were as follows ...
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bioRxiv - Microbiology 2021Quote: ... were mixed with 100 μl of individual peptide pools in 96-well plates and incubated at 37°C for 1 h after which brefeldin A (Sigma Aldrich) was added and incubation continued for 4-5 h ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6J mice were injected subcutaneously in the flanks with 100 μg of MOG35-55 peptide (Polypeptide) emulsified in 100 μl of CFA (Sigma-Aldrich) supplemented with 50 μg of heat-killed Mycobacterium tuberculosis H37Ra (BD Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were reduced with 4 mM DTT for 30 min at 25°C and alkylated by 10 mM iodoacetamide (Sigma-Aldrich) for 45 min in dark in a thermoshaker ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with 500 μg Flag-GST-T6B peptide [46] and 80 μl of anti-Flag M2 Magnetic beads (Sigma, #M8823) for at least 3 hours rotating at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and sDCs were stimulated for 18 h with 1µg/ml of SIINFEKL peptide (OVA 257-264, Proimmune) or with 1mg/ml of low endotoxin soluble ovalbumin protein (sOVA; Sigma-Aldrich) in combination with NaCl or 5 ng/ml of IFNα ...
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bioRxiv - Cell Biology 2022Quote: ... 2% glycerol) and then eluting by shaking with 15 μL of 100 μg/μL of 3X FLAG peptide (Sigma-Aldrich, F4799) in the original lysis buffer for 30 min at 4°C.
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bioRxiv - Molecular Biology 2022Quote: MHC Class I peptides previously identified to bind HLA-A*03:01 (TIAPALVSK) and HLA-B*35:03 (YPTTTISYL) in HCC38BL cells were purchased as ≥95% pure synthetic peptides from Sigma-Aldrich. These peptides were solubilized in DMSO and used for cell treatments at 20 ug/ml concentration ...
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bioRxiv - Biochemistry 2022Quote: ... Each recombinant protein was eluted twice in 250 μL EB containing 1 mM DTT and 250 μg/mL 3×FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).