Labshake search
Citations for Millipore Sigma :
2451 - 2500 of 2558 citations for Recombinant Rat Cntfr His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... The reaction was started after 5 min preincubation by adding 13 µL of a microsome mix from the liver of Sprague-Dawley rats (Sigma Aldrich, 20 mg protein/mL in 0.1 M phosphate buffer ...
-
bioRxiv - Microbiology 2024Quote: The experimental plate for HepG2/C3A cells was prepared by coating with 100 µg/mL of rat tail collagen (Sigma-Aldrich) and 0.02 M glacial acetic acid in PBS for a minimum of 2h at 37°C ...
-
bioRxiv - Pathology 2024Quote: The content of human IgG was quantitated both in the sera and aortic (tissue) homogenates from rats treated with chP3R99 or hR3 mAbs by a commercial ELISA kit (Sigma-Aldrich). Similarly ...
-
bioRxiv - Cell Biology 2023Quote: ... and of PTMs (tyrosinated tubulin: YL ½ antibody, ab6160; rat monoclonal, Abcam; acetylated tubulin: T6793 antibody, clone 6-11B-1, mouse, Sigma-Aldrich; polyglutamylated tubulin ...
-
bioRxiv - Immunology 2023Quote: ... mice were intraperitoneally injected every 48 h with 200 µg monoclonal anti-Ly6G antibody (clone 1A8; Leinco) or 200 µg polyclonal rat serum IgG (Sigma-Aldrich) diluted in sterile PBS beginning at 10 dpi and ending at 34 dpi.
-
bioRxiv - Cell Biology 2023Quote: ... each coverslip was washed 3 times in PBS then placed on top of 50 µL drops of the secondary antibody mixture of goat anti-rat TRITC (1:100; Millipore Sigma) and goat anti-mouse FITC (1:100 ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were blocked at room temperature for 1 hour in 1% casein/PBS and probed overnight at 4°C with a 1:1000 dilution of Roche monoclonal 3F10 rat anti-HA-tag antibody (Sigma 11867423001) and 1:1000 dilution of rabbit anti-P ...
-
bioRxiv - Neuroscience 2023Quote: ... thoracic and lumbar dorsal root ganglia neurons were extracted from E17.5-E18.5 rat embryos and plated on glass coverslip coated with poly-L-ornithine (Sigma-Aldrich, MO, USA) for 4-6 hours before experiments.
-
bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were dissected from postnatal Sprague Dawley rats (P0-P2) and dissociated with 0.25% trypsin (Sigma Aldrich, Merck, Darmstadt, Germany) and 0.004% DNAse (Sigma Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... The membranes were incubated overnight at 4°C with gentle shaking with rat anti-DAT (1:1000, Millipore Cat# MAB369, RRID:AB_2190413), rabbit anti-TH (1:1000 ...
-
bioRxiv - Neuroscience 2023Quote: ... After 1-2 months old rats undergo trans cardiac perfusion with PBSx1 (02-023-5A, Biological Industries) followed by neutral buffered 10% formalin (HT5011, Sigma-Aldrich). The whole brain was removed and immersed in 4% paraformaldehyde (PFA ...
-
bioRxiv - Physiology 2023Quote: ... the supernatant and the fasting and 15 min GTT serum was performed using Rat/Mouse Insulin ELISA kit (EZRMI-13K, Sigma-Aldrich).
-
bioRxiv - Bioengineering 2023Quote: Decellularized rat lung and isolated lobes were perfused with a solution of 0.1% Methylene blue solution (Cat. # 03978, Sigma Aldrich, UK) in PBS either from the trachea or pulmonary artery ...
-
bioRxiv - Cell Biology 2023Quote: Fresh mouse and rat kidneys were fixed by perfusion with 50-60 mL of 4% paraformaldehyde in PBS (158127, Sigma-Aldrich), dehydrated and embedded in paraffin at 58C ...
-
bioRxiv - Neuroscience 2023Quote: ... for 30 min at room temperature and then incubated overnight at 4°C with anti-HA (rat, 1:1000 dilution, Sigma-Aldrich) antibody in TBS containing 10% goat serum ...
-
bioRxiv - Molecular Biology 2023Quote: ... mice and rats (male Sprague-Dawley, aged 7-10 weeks) were subcutaneously injected with the VEGF-receptor inhibitor SU5416 (Sigma-Aldrich) at a dosage of 20 mg/kg body weight under isoflurane anesthesia ...
-
bioRxiv - Neuroscience 2023Quote: ... and the indicated proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
-
bioRxiv - Cell Biology 2023Quote: Human EndoC-βH1 and rat INS1E beta cells were cultured as previously described [45] and incubated with lentiviruses expressing an NNAT-targeting shRNA (Sigma-Aldrich) or Silencer Select siRNAs (Ambion) ...
-
bioRxiv - Developmental Biology 2024Quote: ... rat anti- cytokeratin 8 (TROMA-I; Developmental Studies Hybridoma Bank; 1:500) and rabbit anti- Sox9 (Millipore, Burlington, MA; 1:750). Detection of antibody binding was with the appropriate species-specific Biotin-SP-conjugated goat anti-antibodies (1:400 ...
-
bioRxiv - Neuroscience 2024Quote: ... A rat anti-D1DR monoclonal antibody was used for D1DR-receptor staining (1:500; Sigma-Aldrich, St Louis, MO; Catalog #D2944). To enhance the GFP signals ...
-
bioRxiv - Cell Biology 2024Quote: ... For transfection of human or monkey keratinocytes tissue culture plastic vessels were coated with 20 µg/ml rat Collagen I (Sigma, C3867) overnight at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... the expression levels of recombinant B56 subunit and PME-1 were monitored by western blot using antibodies that specifically recognize HA-tag (Sigma, 2CA5, Cat#: 116666060011, 1:1000) and PME-1(Abcam ...
-
bioRxiv - Plant Biology 2020Quote: ... Prior to labelling the apoplastic fluid was incubated for 30 min with different concentrations of recombinant LpCys1 or chicken egg white cystatin (CEWC, Sigma-Aldrich, St. louis, Mississippi, USA) and Tris-HCl (24 mM Tris ...
-
bioRxiv - Biochemistry 2024Quote: ... the chamber was rinsed three times with T50 to remove non-specific interactions and a protocatechuic acid/recombinant protocatechuate-3,4-dioxygenase (Sigma Aldrich and Oriental Yeast Co., respectfully) (PCA/rPCD ...
-
bioRxiv - Microbiology 2020Quote: ... Primary antibodies were diluted in blocking solution (rat anti-HA clone 3F10, 1:1000; mouse anti-α-tubulin clone DM1A, 1:1000, both from Sigma-Aldrich). Anti-rat Alexa594 ...
-
bioRxiv - Microbiology 2021Quote: ... Membranes were then blocked in 3% BSA in PBS containing 0.2% Tween 20 before staining with rat anti-HA mAb 3F10 (Sigma, diluted 1:1,000) primary antibody in blocking buffer ...
-
bioRxiv - Microbiology 2020Quote: ... primary antibodies were mouse-anti-ROP2/3/4 (1:250 dilution, 4A7, (58)) and rat anti-HA (1:500, clone 3F10, Sigma Aldrich, 11867431001), and secondary antibodies were goat anti-mouse IgG-Alexa 647 (1:2000 dilution ...
-
bioRxiv - Cell Biology 2019Quote: ... For primary antibodies we used hybridoma supernatant of monoclonal antibody 6E3 Rat anti-CLASP2 (Maffini et al., 2009) at 1:150 and Mouse anti-α-tubulin clone B-512 (Sigma Aldrich) at 1:10000 ...
-
bioRxiv - Microbiology 2019Quote: ... were maintained at 37°C on rat collagen diluted at 100µg/mL in water (Cultrex; catalog no. 3443-100-01) in EndoGro medium (Merck Millipore; catalog no. SCME004) supplemented with 5% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were incubated at 14 °C (a temperature not permissible for recycling) with unconjugated goat anti-rat IgG (Sigma, 4 μg/ml) to block labelled channels remaining at the cell surface ...
-
bioRxiv - Microbiology 2020Quote: ... Primary antibodies were diluted in blocking solution (rat anti-HA clone 3F10, 1:1000; mouse anti-α-tubulin clone DM1A, 1:1000, both from Sigma-Aldrich). Anti-rat Alexa594 ...
-
bioRxiv - Neuroscience 2020Quote: ... then incubated with the primary antibody (rat anti-serotonin, Chemicon MAB352, 5/1000; rabbit anti-tyrosine hydroxylase, Sigma Aldrich SAB2701683, 2/1000) in buffer (1% sheep serum ...
-
Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Female rats were administered an intraperitoneal injection of “Hellabrunner Mischung” comprising ketamine hydrochloride/xylazine hydrochloride solution (1ml/kg, Sigma Aldrich, Taufkirchen, Germany). The surgical procedure followed the description of Spicer and colleagues (44) ...
-
bioRxiv - Neuroscience 2020Quote: ... Eluted proteins were resolved by SDS-PAGE and proteins were detected and quantified by immunoblotting: hDAT and DAT/C-S were detected with amino-directed rat anti-DAT (MAB369, Millipore, 1:2000), and N-S/DAT and S/DAT/S were detected with amino-directed mouse anti-hSERT (MAb Technologies,1:2000) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasma insulin and C-peptide were measured with mouse/rat ELISAs (EZRMI-13K and EZRMCP2-21K, respectively, EMD Millipore, Billerica, MA, USA). Plasma non-esterified fatty acids (NEFA ...
-
bioRxiv - Neuroscience 2022Quote: Proteins were resolved by SDS-PAGE and proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in TBS-T followed by incubation in the following primary antibodies that were diluted in TBS-T containing 5% milk: rat-anti-HA 3F10 (Sigma, 1:1,000) and rabbit-anti-aldolase (14 ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% bovine serum albumin (BSA) in PBS-T (0.05% Tween 20) and subsequently probed with the rat anti-HA mAb 3F10 (Sigma, 1:1,000 dilution), followed by biotin-conjugated anti-rat antibody (Roche ...
-
bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
-
bioRxiv - Physiology 2020Quote: DN was induced experimentally in 12-h fasting rats except for the normal group through a single intraperitoneal injection of 0.5 mL STZ (Sigma-Aldrich, Deisenhofen, Germany) at a dosage of 60 mg/kg of body weight ...
-
bioRxiv - Neuroscience 2021Quote: ... (2) rats underwent UVN and received intraperitoneally one L-thyroxine (L-T4) injection (10 µg/kg, dissolved in saline, Sigma, Cat#T2501) at the end of the surgery and one injection/day during the first 3 days post-lesion (UVN-T4 group ...
-
bioRxiv - Cell Biology 2021Quote: ... For KIF18A localization analyses the following primary antibodies were used at the indicated dilutions: rat anti-α-tubulin 1:500 (MAB1864; Sigma Aldrich), rabbit anti-KIF18A 1:100 (A301-080A ...
-
bioRxiv - Molecular Biology 2023Quote: ... Rabbit anti-TgTom40 (van Dooren et al., 2016, 1:2000 dilution) and rat anti-HA high affinity (Sigma, 11867431001, 1:200 dilution) primary antibodies were used ...
-
bioRxiv - Pathology 2024Quote: ... The diabetic rats in the DM+GR group were given a standard diet and were also given glyburide (Sigma-Aldrich; Merk KGaA) at 4 mg/kg in 0.5 ml of 0.5% Tween 80 solution as a supplement ...
-
bioRxiv - Immunology 2023Quote: ... and subsequently incubated with anti-CD16/CD32 (eBioscience, Cat #14-0161-86, clone 93) and 10% rat-IgG1 (Sigma-Aldrich, Cat #I8015) in PBS containing 0.1% BSA (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue morphology was visualized using fluorescent antibodies specific to lymphocyte and neuron specific molecular markers (anti-CD45 [Novus], anti-CD3 [Primary, Bio-Rad], Secondary anti-Rat IgG, [ThermoFisher], and anti-NeuN [Millipore Sigma] ...
-
bioRxiv - Plant Biology 2023Quote: ... The pollen was then washed 3 times in 1x PBS and incubated in a 1:1000 dilution of rabbit anti-rat IgG FITC (Sigma, F1763-.5ML) in 1x PBS+MP for 1 hour ...
-
bioRxiv - Physiology 2023Quote: ... FGF21 was measured by using a commercially available enzyme-linked immunosorbent assay kit (rat/mouse FGF21 enzyme-linked immunosorbent assay kit; Millipore, Billerica, MA), according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: ... The supernatant was collected and serum insulin levels were determined in triplicate using a rat/mouse insulin Enzyme-Linked Sorbent Assay (ELISA) kit from Millipore (EZRMI-13K) according to the instructions of the manufacturer ...
-
bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated for 2 h at RT with secondary antibodies Anti-Rat-IgG conjugated with alkaline phosphatase (AP) (Sigma-Aldrich, USA) at a dilution of 1:1000 in 2.5% BSA in PBS ...