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Citations for Millipore Sigma :
2351 - 2400 of 2558 citations for Recombinant Rat Cntfr His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... Systemic levels of vascular markers were evaluated at the end of the Fc-GDF15 and vehicle treatment of obese ZSF1 rats by using multiplex MILLIplex kits (Millipore). The rat vascular injury panels included VEGF ...
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bioRxiv - Physiology 2020Quote: ... Kidney injury markers KIM-1 and NGAL were evaluated in serum from 20-week-old lean and obese ZSF1 rats by multiplex MILLIplex kits (Millipore). Serum NT-proBNP and rat cardiac injury markers (fatty-acid-binding protein 3 [FABP3] and myosin light chain 3 [Myl3] ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse monoclonal anti-Tau-1 (05-838, 1:1000), mouse monoclonal anti-MAP2 (05-346, 1:500), rat monoclonal anti-tubulin (MAB1864, 1:500) from Millipore; chicken polyclonal anti-GFP (ab13970 ...
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bioRxiv - Neuroscience 2021Quote: Immunohistochemical staining was performed on free-floating brain sections using the following primary antibodies: mouse anti-rat TH (1:4000, MAB318, Merck Millipore), rabbit anti-Girk2 (1:500 ...
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bioRxiv - Microbiology 2020Quote: A549 cells were seeded at 2 × 105 onto cover slides (13-mm diameter; Matsunami) pretreated with coating buffer containing 0.1% collagen (Type I from rat tail; Sigma-Aldrich) and 0.1% gelatin (from bovine bone ...
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bioRxiv - Microbiology 2020Quote: ... Hydrogels were washed with 50 mM HEPES at pH 7.5 and were coated with 200 µL of 0.25 mg/ml rat tail collagen I (Sigma-Aldrich; C3867) in 50 mM HEPES at pH 7.5 overnight at room temperature ...
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bioRxiv - Genomics 2021Quote: ... after which the cells were selected for 10 days on 0.1% gelatine coated 10-cm dishes in 60% Buffalo Rat Liver (BRL)-conditioned medium containing 0.8 μg/mL puromycin (Sigma P9620), 250 μg/mL G418 (ThermoFisher 11811031 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 h at 4 βC with primary antibodies (rat anti-BrdU, 1:500, OBT Serotec; mouse anti-NeuN, 1:200, MAB377, Millipore; rabbit anti-S100β ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then resolved by SDS-PAGE and western blotting was carried out as described in (Hansen et al., 2014) using the following antibodies: DAT (rat MAB369, 1:1000, Millipore) or HA (rat 3F10 ...
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bioRxiv - Systems Biology 2021Quote: INS-1 cells (INS-1 832/3 rat insulinoma cell line) were cultured in RPMI-1640 (Sigma Cat. No. R0883) supplemented with 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... Rats were cardiac perfused with phosphate-buffered saline (0.1 M, pH 7.4) followed by 4% paraformaldehyde (Sigma, St. Louis, MO), brains were removed ...
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bioRxiv - Immunology 2021Quote: ... Freshly isolated cells (4 x 106) were seeded in a pre-prepared gel matrix containing 1 mg/mL rat tail collagen type I (Merck Millipore) in DMEM ...
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bioRxiv - Biophysics 2020Quote: ... Polyclonal rabbit anti-clathrin heavy chain (Cat. Nr. SAB1410212) and rat monoclonal anti-β1-integrin (Cat. Nr. MAB1997) were from Sigma. Rabbit monoclonal anti-caveolin-1 (Cat ...
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bioRxiv - Neuroscience 2020Quote: ... and rats were perfused transcardially with 60 ml of 0.9% saline followed by 120 ml of 4% paraformaldehyde (Sigma-Aldrich). Brains were extracted and post-fixed in 4% paraformaldehyde overnight ...
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bioRxiv - Immunology 2020Quote: ... 8-µm-thick tissue sections were rehydrated and Fc-blocked with normal mouse and rat serums in the presence of 0.3% Triton X100 (Sigma-Aldrich). Tissues were stained with F4/80-APC (Clone BM8 ...
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bioRxiv - Immunology 2020Quote: ... Viral plaques were stained with anti-LCMV nucleoprotein (anti-LCMV NP, clone VL4) and HRP conjugated anti-rat IgG (Millipore) and visualized with O-phenylenediamine (OPD ...
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bioRxiv - Cell Biology 2019Quote: Samples were assayed for insulin or proinsulin content using ELISA according to the instructions of the manufacturers (Rat/Mouse insulin ELISA, Millipore, #EZRMI-13K ...
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bioRxiv - Microbiology 2022Quote: ... Cytokine and chemokine levels were measured with a commercial rat cytokine/chemokine magnetic bead panel 96-well plate assay kit (Milliplex MAP kit, Merck Millipore), which detects 5 cytokines and chemokines including IP-10/CXCL10 ...
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bioRxiv - Cell Biology 2022Quote: ... siRNA reverse transfected cells were grown to confluency before overlaying with neutralised type I collagen (1 mg/ml; Rat tail; Millipore). Collagen was allowed to set for 45 minutes before overlaying with complete growth media ...
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bioRxiv - Bioengineering 2022Quote: ... and the top channel was filled with a mixture of rat tail type I collagen (Sigma Aldrich, St. Louis, MO) at about 100 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000; Sox9, rabbit, Millipore, 1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (rat anti-BrdU, 1:100, Abcam; mouse anti-BrdU, 1:100,BD Biosciences; mouse anti-Nestin, 1:300, Millipore; rabbit anti-GFAP ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells and cultured rat hippocampal neurons overexpressing syt1 C2AB (80-421)-msGFP forming droplets were treated with 5% of 1,6-hexanediol (Sigma, 88571) for 10 min.
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bioRxiv - Microbiology 2024Quote: ELISAs were used to confirm a subset of cytokines identified in the antibody arrays (CCL2, CX3CL1, and Galectin-3 rat ELISA kits, Sigma) using apical or basal supernatants from individual Transwell® inserts from the same experiment than the multiplex array.
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bioRxiv - Neuroscience 2024Quote: ... The antibodies used in this study were as follows: laminin α2 chain (rat monoclonal, 4H8-2, Sigma, Saint Louis, USA), MYOD1 (mouse monoclonal ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed in TBST and blocked again with BSA followed by incubation with anti-rat IgG conjugated with horseradish peroxidase (dilution 1:20000, cat. no. A5795, Sigma) in TBSTB for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal against β-amyloid (6E10, 1:5,000) (Covance, Princeton, NJ), rat monoclonal against phosphorylated TDP-43 (ser409/ser410, 1:1,000) (Millipore, Burlington, MA), and mouse monoclonal against α-synuclein (LB509 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by TBS-Tx (2 × 5 min) and incubated with primary antibody (Rat anti-muscarinic M2 monoclonal antibody, unconjugated, clone m2-2-b3, 1:750, Millipore Cat ...
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bioRxiv - Neuroscience 2022Quote: ... and rat anti-MBP (1:500; Serotec) antibodies (OPCs) or 2 h incubation with mouse anti-GFAP (1:200, Sigma) (astrocytes) ...
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bioRxiv - Neuroscience 2023Quote: ... We used 11NQ anti-ATXN1 as previously described (Al-Ramahi et al., 2006) and rat anti-HA (Sigma, clone 3F10). Following washes ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies coupled to horseradish peroxidase (HRP) were anti-rat-HRP at 1:10000 and anti-mouse-HRP at 1:10000 (Sigma).
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bioRxiv - Biochemistry 2023Quote: ... The membrane was washed 3X with 1x TBST for 5 min each followed by incubation with anti-rat IgG secondary antibody conjugated with horseradish peroxidase (HRP) (Sigma) (1:2000 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... 4- to 5-week-old donor rats were intraperitoneally injected with 30 units of equine chorionic gonadotropin (G4877, Sigma-Aldrich), followed by an intraperitoneal injection of 30 units of human chorionic gonadotropin (C1063 ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated overnight at 4 °C with a rat monoclonal anti-poly(GR) antibody (clone 5A2, MABN778, Millipore), a mouse monoclonal anti-poly(GA ...
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bioRxiv - Pathology 2023Quote: ... cells were seeded at a density of 120000 cells in plugs made of Rat tail collagen I (Millipore; 08-115) in a 24-well plate and treatment done with Control ...
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bioRxiv - Cell Biology 2023Quote: The TAA model of cirrhosis was induced as described.25 Male Wistar rats received ip injections with 200 mg/kg TAA (Sigma) twice a week for 12 weeks.
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bioRxiv - Neuroscience 2023Quote: ... Tg-AD and WT rats were fed a combination of DZ (10 mg/kg bw/day, Sigma Aldrich cat# D9035) and DIB (200 mg/kg bw/day ...
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bioRxiv - Neuroscience 2023Quote: BV2 and primary rat glial cells were fixed for 15 min in 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA) and washed with PBS ...
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bioRxiv - Cell Biology 2023Quote: The DEN model of cirrhosis was prepared as previously described.23 Male Wistar rats received weekly intraperitoneal (ip) injections of DEN (Sigma) at 50 mg/kg for 18 weeks.
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended and plated onto 10 µg/mL fibronectin- or rat tail collagen type I-coated glass bottom dishes (Sigma). Cells were fixed the next day with 4% paraformaldehyde ...
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bioRxiv - Plant Biology 2024Quote: ... and labelled with the secondary antibody (diluted 1:50 in 0.1% BSA in PBS, Anti-Rat-IgG – Gold antibody; Sigma, USA) for 1 h at 37°C ...
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bioRxiv - Physiology 2024Quote: ... Serum samples were stored at −80 °C until assessed by ELISA according to manufacturer’s instructions (Ultrasensitive Human Growth Hormone ELISA, 22-HGHHUU-E01, ALPCO; mGH, EZRMGH-45K and Rat/Mouse Insulin ELISA, EZRMI-13K, Millipore).
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bioRxiv - Cell Biology 2024Quote: ... cells were dislodged with 1x trypsin (HiMedia) and cultured on polyacrylamide (PA) hydrogels coated with rat tail Collagen type I (Sigma) and/or low molecular weight (8-15 kDa ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies coupled to horse radish peroxidase (goat anti-rabbit, goat anti-rat, and goat anti-mouse) were purchased from Sigma and used at the recommended dilution.
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bioRxiv - Neuroscience 2024Quote: ... followed by the addition of 50 µl of a 1 µg/ml rat anti-TREM2 detection antibody (#MABN2321, Sigma Aldrich) into the wells ...
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bioRxiv - Cell Biology 2024Quote: ... His-tagged PRDX6 from rat (gene cloned into pET15b NdeI and XhoI restriction sites) as produced in the Escherichia coli strain Origami (DE3) (Novagen). 20 µL of Prdx6- Pet15b transformed Origami (DE3 ...
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bioRxiv - Bioengineering 2021Quote: ... Marimastat ((2S,3R)-N-[(1S)-2,2-dimethyl-1-(methylcarbamoyl)propyl]-N’,2-dihydroxy-3-(2-methylpropyl)butanediamide), ɑ2-antiplasmin (SRP6313-100) and recombinant plasminogen activator inhibitor 1 (PAI-1, 528208) were purchased from Sigma-Aldrich (Saint-Louis, MI). Sodium alginate (SA ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM recombinant calmodulin (Enzo, cat. BML-SE325-0001) and 2 mM Adenosine 5’-triphosphate (ATP) disodium salt hydrate (Sigma-Aldrich, cat. A1852-1VL) for 30 min at 37 °C ...