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Citations for Millipore Sigma :
2151 - 2200 of 2558 citations for Recombinant Rat Cntfr His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Microbiology 2020Quote: ... mouse polyclonal antibody was produced immunizing a mouse with 50 mg of purified recombinant MtEPSPS containing Freund’s incomplete adjuvant (Sigma-Aldrich, USA) (total volume of 100 µL ...
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bioRxiv - Developmental Biology 2022Quote: ... The dish was then incubated with 40 µg/mL mouse E-cadherin recombinant protein (8875-EC-050, Bio-Techne) or 40 µg/mL fibronectin (F1141, Sigma-Aldrich) at 4 □ overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... The chopped and digested pieces were placed in a serrated 60mm petri dishes and supplemented with 10ng/μl each of human recombinant epidermal growth factor (hEGF, Cat # E9644, Sigma-Aldrich), N2 suppliment-1X (Cat # 17502048 ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2021Quote: ... The recombinant LymCBP HAT domain (200 ng) were incubated with the histone H1 purified from calf thymus (Sigma-Aldrich, 1 μg) and 7.4 kBq of [14C]-acetyl-CoA (Moravek ...
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bioRxiv - Microbiology 2021Quote: ... Sublines expressing a doxycycline-inducible BZLF1 transactivator were produced by culturing 106 LCL cells with the recombinant lentivirus in presence of 8 μg/ml polybrene (TR-1003-G, Sigma-Aldrich) for 24 hours followed by replacement of the infection medium with fresh complete medium ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Biochemistry 2019Quote: ... recombinant mTLR9-ECD was titrated into a solution of oligonucleotide labeled at the 5’ end with Alexa 488 Fluor (Sigma-Aldrich). The oligonucleotide concentration ...
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bioRxiv - Zoology 2020Quote: ... Each lysate was confirmed to express the appropriate recombinant protein at the expected size using an anti-V5 antibody produced in rabbit (Sigma V8137). Isoform specificity was tested via immunoblotting all cell lysates (empty vector control ...
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bioRxiv - Genomics 2020Quote: ... RPMI-1640 medium used to culture the MCF7 cells was additionally supplemented with 0.01 mg/ml human recombinant insulin (Sigma Aldrich, #I3536).
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bioRxiv - Cell Biology 2021Quote: ... 250 µl of maintenance medium (50% Wnt3a-conditioned medium (ATCC#CRL-2647, Manassas, VA, USA) and 50% of 2× basal medium supplemented with recombinant human EGF (Sigma-Aldrich), recombinant human noggin (R&D Systems ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Cell Biology 2020Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Neuroscience 2021Quote: ... αSyn oligomers were produced by incubating recombinant αSyn to a final concentration of 20 µM with 300 µM DOPAL or dopamine (Sigma-Aldrich) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mg of protein from each sample was incubated for two hours with purified Multidsk2 recombinant protein bound to glutathione agarose magnetic beads (Sigma G0924). After incubation beads were washed four times with D-buffer and resuspended in NuPage LDS sample buffer (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... The bacterial culture was then induced to produce the recombinant protein by the addition of 0.5 M isopropyl β-d-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) and was allowed to grow for a further 3 h ...
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bioRxiv - Immunology 2021Quote: ... and 2 ml Flt-3L-containing supernatant [produced from SP2/0 transfected cell line secreting mouse recombinant Flt-3L42 for 7 days at 37°C until activation with 100 ng/mL LPS (Sigma-Aldrich) 18 hours prior to the assays.
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
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bioRxiv - Cell Biology 2021Quote: ... 5a), recombinant mEGFP(−7e) or scGFP(+7e) were dissolved in injection buffer (50 mM K-HEPES, pH 7.4 (Sigma-Aldrich, H3375), 25% glycerol (Applichem ...
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bioRxiv - Molecular Biology 2022Quote: ... the native or reconstituted chromatin samples (140 μg DNA/ml) in HNE were treated with 25 μg/ml human recombinant PAD4 enzyme (Sigma Aldrich cat # SRP0329 or Cayman Chemical cat ...
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bioRxiv - Microbiology 2022Quote: ... The LDH assay was carried out in 96 or 384 well plate format and a recombinant porcine LDH enzyme (Sigma-Aldrich) was used in each LDH assay plate in a serial dilution to extrapolate a standard curve which was used to estimate LDH released on infection with A ...
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bioRxiv - Neuroscience 2024Quote: ... lateral and medial) were each injected with 10 μl of either PBS or recombinant mouse GDNF (10 μg/ml, Sigma-Aldrich) using 31-gauge insulin syringes without damaging any innervating nerves ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µM carnitine) with or without supplementation of 100 ng/ml (13.16 nmol/l) IGF1 (human recombinant IGF1, I3769, Sigma-Aldrich, Germany). Medium was changed three times per week and 48 hours before harvest ...
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bioRxiv - Biochemistry 2024Quote: ... were mixed with 60 ng of recombinant HA-BACH1 in 21 μl of a reaction buffer containing 10 μg of bovine ubiquitin (Sigma-Aldrich), 2 mM ATP ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were seeded on 6-well cell culture plate wells at a density of ∼2 x 105 cells/well with 2 mL of the culture medium containing 103 units/mL ESGRO Recombinant Mouse Leukemia inhibitory factor (LIF) (Millipore #ESG1107) and 2i (1.0 µM PD0325901 Toris #4423 and 3.0 µM CHIR99021 Wako #038-23101) ...
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bioRxiv - Cancer Biology 2022Quote: ... 7μL of each dilution was then used in SDS-PAGE alongside a dilution series of recombinant GST protein (Sigma-Aldrich #SRP5348) from 5ng-200ng ...
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bioRxiv - Microbiology 2023Quote: ... mixture of BEI-EMCV strain SING-M105 or recombinant VP1 protein prepared according or BEI-EMCV with Complete Freund’s adjuvant (Sigma-Aldrich, USA) was injected intramuscularly into five BALB/c mice at the concentration of 50 μg of antigen per mouse ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein expression was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma; 16°C, 200 RPM, 20 hours). Cells were harvested by centrifugation (5000 RPM ...
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bioRxiv - Bioengineering 2023Quote: ... The hybridized RNAs were then mixed with 25 µL of 30 µM (5 mg/mL) recombinant SpCas9 protein (Sigma-Aldrich #Cas9PROT) for 30 minutes at 4 C ...
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bioRxiv - Cancer Biology 2023Quote: ... stable PDA cell lines were generated by infecting 70% confluent cultures with the recombinant lentiviral vectors with 10 mg/mL polybrene (TR-1003, Sigma-Aldrich) twice over 2 consecutive days ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant Human Protein #PHG0264), 10 ng/ml epithelial growth factor (EGF, Recombinant Human Protein #PHG0311) and 10 µM ROCK Inhibitor Y-27632 (Sigma, #Y0503) were added to the media freshly before use ...
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bioRxiv - Cell Biology 2024Quote: In vitro kinase assays were performed in the presence or absence of different recombinant human atypical PKC (50 or 100 ng of PKC ζ, #14-525 Merk Millipore; 1µg of PKC I/αPar6 complex ...
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bioRxiv - Physiology 2024Quote: ... and 120 min after intraperitoneal injection of 1g/kg glucose (glucose tolerance test) or 0.5 U/kg of recombinant human insulin (Sigma Cat.# I6634) (insulin tolerance test).
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bioRxiv - Microbiology 2019Quote: ... Membranes were probed with rat anti-HA antibodies (1:100 to 1:3,000 dilutions; clone 3F10, Sigma, 11867423001), rabbit anti-TgTom40 [44] (1:2,000 dilution) ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then blocked with ultra-block solution (10% rat sera, 10% hamster sera, 10% mouse sera (Sigma), and 10 μg/ml of anti-CD16/32 (2.4G2 ...
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bioRxiv - Neuroscience 2019Quote: ... Between Week 27 and 29 all rats received ascending concentrations of citric acid (CA, Sigma Aldrich, cat# PHR1071) at 1 ...
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bioRxiv - Physiology 2020Quote: ... MHz After the echo examination the rats were anaesthetized with urethane (1.5 g/kg b.w. i.p., Sigma-Aldrich) and left femoral artery was cannulated with polyurethane catheter for arterial blood pressure (ABP ...
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bioRxiv - Developmental Biology 2019Quote: ... Washed beads were added to rat forebrain extracts (RFE) containing either anti-PDI (Sigma-Aldrich P7496 Lot#054K4801), non-immune rabbit IgG or bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2020Quote: ... Rats were exposed for 24 h to a bottle containing a sucrose solution (5% in tap water, Sigma), placed in the wire-top cage cover adjacent to standard tap water ...
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bioRxiv - Neuroscience 2021Quote: ... Signal-positive cells were detected by the following secondary antibodies: anti-rat Cy3 (Millipore, cat#AP189C, 1:250), anti-chicken Alexa Fluor 488 (Invitrogen cat#A11039 ...
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bioRxiv - Neuroscience 2021Quote: ... rats were injected intraperitoneally with 100 mg/kg 5-Bromo-2′-deoxyuridine (BrdU, Sigma-Aldrich, St. Louis, MI) dissolved in warm 0.9% saline immediately after release from Decapicone bags ...
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bioRxiv - Neuroscience 2019Quote: ... P0 rat hippocampal neurons were plated to poly-D-lysine (PDL; 10μg/cm2; Millipore Cat# A-003-E) coated coverslips at 5×104 neurons per well in a 24-well plate ...
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bioRxiv - Microbiology 2020Quote: ... rat monoclonal anti-CD45 antibody at a dilution of 1:100 (05-1416, Millipore Sigma, Burlington, MA, USA), rabbit polyclonal anti-CD68 at a dilution of 1:200 (ab125212 ...
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bioRxiv - Biochemistry 2022Quote: ... Colo205 cells were densely plated on 96-well plates precoated with 0.1μg/mL rat-tail collagen (Sigma-Aldrich) overnight and treated with activating concentrations of Fabs for 5 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The rats received daily IV infusions of the antibiotic Gentamycin (4 mg/kg, Sigma-Aldrich, St. Louis, MO) for seven days ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies for another series included rat anti-glial fibrillary acidic protein (1:2500, AB5804, Millipore, Lot # TA265137) or goat anti-Iba1 (1:1000 ...
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bioRxiv - Systems Biology 2021Quote: ... we plated 10k – 30k cells on 12 mm collagen-coated glass coverslips (rat tail collagen, Sigma, #122-20). Cells were plated the afternoon prior to imaging ...