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Citations for Millipore Sigma :
2201 - 2250 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 10 ml lysis suspension were then incubated for 30 min at RT with 20 μl heme solution (100 mg heme (Sigma Aldrich) dissolved in 6 ml NaOH) ...
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bioRxiv - Neuroscience 2023Quote: ... BP151-500) in 1X PBS then blocked for 2 hours at RT with 2% bovine serum albumin (BSA, Sigma-Aldrich, A2153), 0.1% Triton in 1X PBS ...
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bioRxiv - Neuroscience 2023Quote: Slices were blocked for 2h at room temperature (RT) in a solution containing 3% bovine serum albumin (BSA,A7906 Sigma-Aldrich) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... 2 washes of 100% DCM (15 minutes each at RT) were performed before placing samples in dibenzyl ether (DBE, Sigma 108014) for clearing and imaging ...
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bioRxiv - Neuroscience 2022Quote: ... After incubation of primary antibodies at 4°C overnight and secondary antibodies for 1 h at RT (Alexa Flour 488 and 594) cells were embedded in Mowiol (Sigma-Aldrich) and subjected to imaging with the software Axiovision 4.8 at the microscope Axio Observer Z.1.
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bioRxiv - Molecular Biology 2022Quote: ... then alkylated for 15 min at RT in the dark with 10 mg/mL iodoacetamide (Sigma-Aldrich, St. Louis, MO, USA). The protein mixture was then digested overnight with trypsin ...
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bioRxiv - Neuroscience 2023Quote: ... Then, samples were incubated with streptavidin-phycoerythrin (30 min, RT) and before measurements on a Luminex MAGPIX® System (Merck-Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... The tumours were then permeabilised in 70% EtOH for 2 h at RT and then blocked with blocking buffer [1% BSA (Sigma Aldrich), 0.2% Triton-X (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... fixed with 2% paraformaldehyde (PFA) in PHEM and washed once for 5 min at (RT) with 3% BSA (bovine serum albumin, Merck-Sigma, Germany) in Tris-buffered saline with 10 mM EGTA and 2 mM MgCl2 (TBSTEM) ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was stained with DAPI (1 mg/ml in water) for 5 min at RT and coverslips were mounted in fluoroshield (Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: Retinal cryosections were rehydrated in 1X phosphate buffered saline (1X PBS) for 20 minutes at room temperature (RT) followed by incubation in 1.75 µg/mL Nile Red (Sigma, Cat# N3013) in 100% acetone for 1-hour at RT in the dark ...
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bioRxiv - Immunology 2024Quote: Tissues were then washed with wash buffer and blocked for 11 hours at RT with 1X TBS with 5% (v/v) normal donkey serum (Sigma-Aldrich). The first antibody cocktail was prepared in 1X TBS-T 5% (v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were incubated for 45 min at RT in the dark in imidazole buffer (0.1 M imidazole (Sigma-Aldrich, Cat# I5513) in PBT supplemented with 1% H2O2 ...
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bioRxiv - Cell Biology 2024Quote: ... iPSC-CMs were cultured on Matrigel-coated coverslips and fixed at day 60 of differentiation in 4% Roti-Histofix (Carl Roth) at RT for 10 min and blocked with 1% Bovine Serum Albumin (BSA; Sigma-Aldrich) in PBS (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... They were blocked in 1% BSA in PBT for 1hr at RT and incubated overnight at 4°C with the monoclonal mouse anti-FLAG M2 antibody (Sigma, F1804) diluted 1:500 in 1% BSA/PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Supernatant was discarded and sample was resuspended in room temperature (RT) Liver Digest Buffer [0.15 mg/mL collagenase type XI (Sigma Aldrich; C9407), 0.3 U/mL dispase (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... blots were blocked 1 h at room temperature (RT) with 5% non-fat dry milk (Carl Roth, T145.2) or 5% bovine serum albumin (BSA, Sigma-Aldrich, A7906) in TBS-Tween (0.5 M Tris ...
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bioRxiv - Developmental Biology 2023Quote: ... all samples were incubated with Blocking One Histo (Nacalai Tesque) for 10 min at RT and 10% normal donkey serum (Sigma-Aldrich) in PBS/0.5% Triton X-100 with 5% Blocking One Histo for 30 min at RT.
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bioRxiv - Biophysics 2023Quote: Immunofluorescence was performed on adhered cells after fixation with 4% PFA at room temperature (RT) for 10 min and permeabilization with 0.5% Triton® X-100 (Sigma-Aldrich) at RT for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... in PBS 1X at RT for 40 min and incubated with a blocking buffer containing 3% bovine serum albumin (BSA, Sigma Aldrich) in PBS 1X RT for 40 min ...
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bioRxiv - Genomics 2023Quote: ... we first infected MCF7 cells with EF1a-nCas9/RT lentivirus followed by treatment with puromycin (2.5 µg/ml; Sigma-Aldrich, P8833) for 2 weeks to eliminate uninfected cells ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were then permeabilized for 20Lmin in PBS containing 0.1% Triton X-100 and 10% goat serum (GS) at RT and immunostained with a guinea-pig polyclonal anti-vglut1 (1/4000; Millipore AB5905), a mouse monoclonal anti-BIII tubulin (1/1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... cleared biopsies were blocked for 3–4 h at room temperature (RT) or overnight at 4°C in blocking buffer (PBS (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed once with PBS for 10 min followed by a blocking- permeabilization step for 1 hr at RT in blocking buffer (PBS/ 5% donkey serum Bio-Rad C06SB / 0.1% Triton Sigma X100). Primary antibodies diluted in blocking buffer were then added to the cells (Table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... and during the secondary antibody incubations (60’ at RT in dark) DAPI was included as a nuclear counterstain (1ug/mL; Sigma Aldrich). Directly conjugated primary antibodies were incubated as a final step prior to washing and mounting (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... An additional washing step was followed by a 1 h incubation at RT with the primary mouse anti-c-myc antibody (500 µl, 1:200, Sigma Aldrich). Further ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed thrice with 1×PBS and incubated for 15 min at RT with 0.1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, Poland). After a final three washes with 1×PBS ...
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bioRxiv - Microbiology 2023Quote: ... Incubation with primary antibodies (1 h, RT) was followed by washing and incubation with secondary antibodies and DAPI (25 µg ml-1, Sigma-Aldrich) (1 h ...
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bioRxiv - Cell Biology 2022Quote: ... used at 1% for 10 min at RT allowed cell permeabilization and a solution of bovine serum albumin (BSA; Sigma-Aldrich) 2% ...
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bioRxiv - Microbiology 2023Quote: ... plated on fluorodishes were incubated in 50mM NH4Cl for 5min at room temperature (RT) to quench free aldehydes and in 1X PBS containing 3% Bovine Serum Albumin (BSA, Sigma-Aldrich) for 15min at RT to block non-specific epitopes ...
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bioRxiv - Neuroscience 2023Quote: ... and 5% normal goat serum in MAB for 30 min at RT and incubated with alkaline phosphatase-conjugated anti-DIG antibody (1:10000 for oxt, 1:1000 for other genes, Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed with PBS and incubated for 15 min at RT in PBS with DAPI (1:400; Sigma-Aldrich, USA). Following washes with PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... were harvested and fixed for 1 h at RT with 2% PFA and Glutaraldehyde in 0.1M phosphate buffer pH7 and 1% sucrose (all from Sigma-Aldrich, USA). Next ...
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bioRxiv - Immunology 2023Quote: ... in PBS for at least 30 min at 37 °C and permeabilized with PBS containing 0.1 % Triton-X for 5 minutes and blocked with blocking solution (3 % BSA in PBS) at RT for 30 minutes (all reagents from Sigma-Aldrich). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... permeabilized with 0.25%Triton X-100/PBS for 15 min and blocked for 1 h at RT with 5% NGS (Jackson Immuno Research, 005-000-121) + 5% BSA (Sigma, A2153). The following primary antibodies were used ...
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bioRxiv - Microbiology 2023Quote: ... was added for 1h at RT followed by incubation with OPD substrate revealing reaction for 10 min (Sigma-Aldrich, MO, USA). Absorbances were analyzed at 495 vs 620 nm on an ELISA plate reader (Berthold ...
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bioRxiv - Cell Biology 2023Quote: ... the aortic tissue was incubated for at least 12 h in Roti®Histol (#6640.4, Carl Roth) at RT and embedded in paraffin (#P3558, Sigma-Aldrich).
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at RT with a rabbit polyclonal anti-pH3 antibody (1:500; Sigma; Cat#: H0412; RRID: AB_477043). Sections were then rinsed 3 times in TBS for 15 min each and incubated for 1 hour at room temperature with a Cy3-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch ...
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bioRxiv - Developmental Biology 2024Quote: ... Tween-20 in PBS for 15 min at RT and then incubated with rabbit anti-human RYBP polyclonal antibody (Sigma, HPA053357) and mouse anti-human hCG-β antibody (abcam ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... then incubated for 30 min at RT with blocking buffer (1% Bovine Serum Albumin in 1X PBS) and further incubated for at least 1 hour at RT with rabbit anti-hACE2 antibody (SAB 3500978, Sigma-Aldrich) or rabbit anti-TMPRSS2 antibody (BS-6285R ...
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bioRxiv - Biochemistry 2024Quote: Cells were harvested by centrifugation at 2000 x g for 15 minutes at RT and resuspended in Lysis Buffer (1X BugBuster® Protein Extraction Reagent (Merck-Millipore), 20 mM Tris ...
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bioRxiv - Immunology 2024Quote: ... Tetramer staining was performed in combination with staining for chemokine receptors CXCR5 and CXCR6 for 1 h at RT in staining buffer supplemented with Dasatinib (50 nM) and biotin (20 nM; Sigma, B4501). After washing of the samples with FACS buffer (PBS supplemented with 1 % FCS) ...
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bioRxiv - Cell Biology 2024Quote: The samples were fixed in 4% PFA for 1 h at room temperature (RT) and then incubated with 15% sucrose solution (Sigma Aldrich) supplemented with 0.03% eosin (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... were incubated for 20 min at RT in 10 ml of hypotonic solution (75 mM KCl, 10 mM MgSO4, 0.5 mM spermidine trihydrochloride (Sigma-Aldrich, S2501), 0.2 mM spermine tetrahydrochloride (Sigma-Aldrich ...
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bioRxiv - Genetics 2024Quote: ... 10 µg of total protein was reduced by incubation at RT for 20 minutes with 1 µl 10 mM dithiotrytol (Sigma-Aldrich) followed by incubation for 20 minutes at RT in the dark with 1 µl 50 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2024Quote: ... nitrocellulose membranes were incubated at RT for 2 h with anti-GFP (Miltenyi 130-091-833) or anti-Flag (Sigma A8592) antibodies conjugated with horseradish peroxidase in TBS-T at 1:5000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Samples for qRT-PCR were homogenized in TRiZOL (Sigma Aldrich) reagent and further processed for RNA extractions.
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bioRxiv - Biophysics 2020Quote: ... PCR was performed with KOD Hot Start DNA polymerase (Novagen) and oligonucleotides were synthesized by Sigma Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... PCR was completed using RedExtract ReadyAmp Taq (Sigma, SIG-R4775) with the following cycle conditions (29 cycles) ...
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bioRxiv - Immunology 2019Quote: ... Indexed PCR product was separated on 2%-LMP agarose (Sigma) and purified with the Gel Extraction Kit (Qiagen).