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Citations for Millipore Sigma :
2401 - 2450 of 2557 citations for SCAMP1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... cells were permeabilized for 1 min with 0.1% Triton X-100 in PBS and saturated with blocking buffer (1% BSA in PBS) before incubation for one hour with anti-α-Tubulin (T5168. SIGMA; 1:2.000) in PBS containing 1% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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bioRxiv - Neuroscience 2020Quote: ... sectioned organoids were washed three times with 1x PBS for 10 minutes at RT before blocking for 1 hour with 3% bovine serum albumin (BSA) (Sigma, Cat. A9418-50G) and 0.1% triton X-100 in 1x PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Monolayers were permeabilized with 0.5% Triton X-100 and blocked with a blocking buffer containing 10% goat serum (cat# G9023, Sigma-Aldrich, St. Louis, MO) and 1% bovine serum albumin (cat# A3803 ...
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bioRxiv - Neuroscience 2020Quote: ... and treated with blocking solution containing 1 % bovine serum albumin (Fisher Bioreagents, Cat.# BP1605-100) and 10 % normal goat serum (Sigma-Aldrich, Cat.# G9023) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Slots were washed with one volume of DPBS before washing the whole membrane with TBS and blocking with 5% skimmed milk powder (Sigma-Aldrich, Munich, Germany) in TBS for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed three times with 1X PBS prior to blocking with 3% bovine serum albumin (BSA; Sigma #A7906, Saint Louis, MO) in 1X PBS ...
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bioRxiv - Microbiology 2021Quote: ... Organoid sections were air dried and washed three times for 10 minutes at RT followed by blocking and permeabilizing for 60 mins with a solution containing 3% bovine serum albumin (Sigma, Cat. A9418-50G) and 0.1% Triton X-100 in 1XPBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were blocked in 1:1 mixture of 1XPBS : Intercept PBS blocking buffer (LiCor) and then incubated with anti-PAP (Sigma, P1291, lot 92557) or anti-MYC (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... The wells were subsequently blocked for 1 hr in blocking solution (25 µL of 1% BSA [Sigma], 0.2% Tween-20 in 1x PBS) then incubated with 25 µL of the Click-iT EdU detection mix (prepared as per manufacturer’s instructions using Alex Fluor 455® ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were incubated overnight in primary antibody diluted in blocking buffer (1:1000 anti-cleaved caspase-3 Cell Signaling Technologies #9664, 1:4000 anti-α-Tubulin Sigma #T5168 ...
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bioRxiv - Cell Biology 2022Quote: ... The tissue sections where fixed in -20 °C methanol for ten minutes followed by a blocking cycle (5×3 min) using phosphate buffered saline containing 1% bovine serum albumin (Sigma-Aldrich, Copenhagen, Denmark). The samples where then incubated overnight at 4 °C with the primary antibodies (dilution factor ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.1% Triton X-100) at room temperature and blocked in KTBT + 20% sheep serum + 2% Blocking Reagent (11096176001, Sigma-Aldrich, St. Louis, MO) for 4 h ...
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bioRxiv - Neuroscience 2019Quote: ... Free-floating slices were washed for 3 × 10 min periods in PBS before blocking and permeabilization for 2 h with 0.3 % Triton X-100 in PBS and 4% normal goat serum (Sigma-Aldrich, Oakville, Ontario, Canada). Slices were incubated with streptavidin conjugated with Alexa Fluor 555 or 546 (1:1000 ...
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bioRxiv - Developmental Biology 2019Quote: ... 0.1% Triton X-100) at room temperature and blocked in KTBT + 20% sheep serum + 2% Blocking Reagent (11096176001, Sigma-Aldrich, St. Louis, MO) for 4 h ...
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bioRxiv - Neuroscience 2021Quote: ... and rehydrated for 20 mins with 1X PBS, followed by blocking buffer containing permeabilization solution (Myers et al., 2014) with either 5% normal donkey (EMD Millipore, S30-100ml) or goat serum (Thermofisher scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... in PBS (blocking buffer) was used to block the cells for one hour and replaced with anti-FLAG antibodies (Sigma Aldrich, F-1804) diluted in blocking buffer (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... at least 3 times and incubated at room temperature for 2 h in blocking solution containing 5% normal horse serum (Sigma-Aldrich, Cat. # H0146) in 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Thereafter the cells were incubated at 4 °C overnight in blocking-solution containing the primary antibody (Rabbit anti-hemoglobin (SIGMA, Cat. No. H4890) diluted 1/1000) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were well washed with 1x PBS to remove permeabilization solution before blocking with 3% w/v BSA (Sigma-Aldrich, Cat # A7906-100G) in 1x PBS overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... fluorophore-conjugated secondary antibodies were incubated at room temperature for 30-60 minutes in blocking buffer together with DAPI (Sigma-Adrich,1μg/ml). All washes were performed in Tween/PBS (0.2%) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti-TH antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 44-48 hr ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti DsRed polyclonal antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 18-22 hr ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 72-hours in the same blocking solution with rabbit anti-CRF (Peninsula,T-4037, 1:1K) and mouse anti-TH (Millipore, MAB318, 1:5K) at 4°C on a shaker followed by 24-hours at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C following incubation with mouse anti-rAgApyrase antibody (1:10,000 in blocking buffer) or rabbit anti-tPA antibodies (1:2500) (Millipore-Sigma, Marlborough, MA, USA). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were permeabilized and blocked for an hour at room temperature using blocking buffer made fresh with the following ingredients: PBS containing 10% donkey serum (Sigma-Aldrich, catalog #D9663), 10% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... in a staining jar and incubated for 2 h at room temperature in blocking solution containing 5% normal goat serum (Millipore Sigma, Item# G6767) in 1% Triton X-100 (Millipore Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... sectioned organoids were washed three times with 1x PBS for 10 minutes at RT before blocking for 1 hour with 3% bovine serum albumin (BSA) (Sigma, Cat. A9418-50G) and 0.1% triton X-100 in 1x PBS ...
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bioRxiv - Cell Biology 2022Quote: ... The transferred membrane was briefly washed with TBST (0.1% Tween-20 in TBS) and blocked with Blocking Buffer (5% Blotting-Grade Blocker [1706404, BioRad] or 5% BSA [A9647-50G, Sigma-Aldrich] in TBST) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... diluted in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin, Sigma-Aldrich in PBS) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated overnight at 4°C with primary antibody in blocking solution : anti-vinculin (Sigma-Aldrich, catalogue no. V9131, dilution 1:400) followed by three washes with PBS Tween 0.2% ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with blocking buffer (PBST containing 10% normal goat serum [Thermo Fisher Scientific, 16-210-064] or donkey serum [Sigma-Aldrich, D9663]) at room temperature for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... For IHC we permeabilized and blocked sections with blocking solution (PBS containing 5% normal goat serum [Thermo Fisher Scientific # 10000C] and 0.1% Triton X-100 [Millipore-Sigma # X100-100ML]) for 60 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed with PBS (3x10 min at room temperature) and incubated for 2 hours at room temperature in blocking solution containing 5% normal goat serum (Millipore Sigma, Item # G6767) in 1% Triton X-100 (Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections were then blocked for 30 min at room temperature in blocking buffer containing 1% (wt/vol) BSA (Sigma-Aldrich, A9647-100G), 22.52 mg/mL glycine (AmericanBio ...
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bioRxiv - Genetics 2023Quote: ... then blocking solution was added to each well consisting of PBS containing 0.5% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA). Cellswere incubated in blocking solution for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were diluted in blocking solution and incubated for 1 h at room temperature (anti-E-cadherin, Cell Signaling Technology, 96743; anti-pan-cytokeratin, Millipore Sigma, C5992 ...
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bioRxiv - Neuroscience 2024Quote: ... floating sections were washed in phosphate-buffered saline (PBS) and blocked for 1 hour at room temperature in a blocking buffer of 10% normal donkey serum (Millipore, cat# S30-100ML) and 1% Triton X-100 in TBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The following antibodies used for immunocytochemistry as described above are as follows: mouse monoclonal antibody to FLAG peptide (Sigma #F1804, 1:500), sheep polyclonal to TGN46 (Biorad #AHP500G ...
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bioRxiv - Molecular Biology 2020Quote: The following antibodies used for immunoblot as described above are as follows: mouse monoclonal antibody to FLAG peptide (Sigma #F1804, 1:5000), Rabbit monoclonal to c-Myc (abcam #ab32072 ...
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bioRxiv - Bioengineering 2019Quote: ... Hela cells from the same batch of 2D culture were seeded into the peptide hydrogel with density ~ 8 × 104 which was measured by an automated cell counter (Millipore Corporation, PHCC3000). The 3D culture was maintained using a 24-well cell culture plate with ~ 390 μL cell suspension ...
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bioRxiv - Cancer Biology 2021Quote: ... and calmodulin-binding peptide (CBP) was affinity-purified by using streptavidin-conjugated agarose beads (EZview™ Red Streptavidin Affinity Gel, Sigma-Aldrich). The initial protein peaks containing EP400NL were collected and pooled together after the size exclusion chromatography and incubated with streptavidin affinity beads at 4 °C overnight with constant rotation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasma insulin and C-peptide were measured with mouse/rat ELISAs (EZRMI-13K and EZRMCP2-21K, respectively, EMD Millipore, Billerica, MA, USA). Plasma non-esterified fatty acids (NEFA ...
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bioRxiv - Biophysics 2022Quote: ... about 100 μL supernatant was left and the bound protein was eluted by incubation with 10 μL FLAG peptide (Sigma-Aldrich F3290) at 4°C for 3 hours ...
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bioRxiv - Biochemistry 2021Quote: ... 12 μg peptides were adjusted to pH 7-8 by adding one volume of 50 mM ammonium bicarbonate (ABC, Sigma-Aldrich, A6141). Offline fractionation by high-pH reversed-phase chromatography was carried out on an Ultimate 3000 HPLC (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The source voltage and focusing voltages were tuned for the transmission of MRFA peptide (m/z 524) (Sigma-Aldrich, St. Louis, MO). Data-dependent analysis was carried out in orbitrap-IT parallel mode using CID fragmentation on the five most abundant ions in each cycle ...
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bioRxiv - Biophysics 2020Quote: ... Approximately 10 pmol of peptides were loaded onto the Ascentis Peptides ES-C18 column (2.1 mm x 100 mm, 2.7 μm particle size; Sigma-Aldrich, St. Louis, MO) at 0.400 mL/min flow rate and were eluted with the following gradient ...
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bioRxiv - Genetics 2020Quote: ... The Rad21l1 peptide was concentrated to a final concentration of 1 mg/ml in PBS using a 10 kDa centrifugal filter (Sigma, Catalog # UFC901008). The Rad21l1-derived peptide was injected into three guinea pigs by Pocono Rabbit Farm and Laboratory following the 91-day polyclonal antibody production protocol.
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bioRxiv - Cell Biology 2021Quote: ... The beads were then extensively washed in lysis buffer and elution was carried out with 50 μL of 3×FLAG peptide (Sigma-Aldrich #F4799), the supernatant was separated from the beads by centrifugation ...