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Citations for Millipore Sigma :
2401 - 2450 of 3014 citations for 6 bromochromone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Pharmacological experiments were carried out on PCs during simultaneous somatic and dendritic recordings after 10 minutes of control recording using ACSF with the following drugs: 20 µM 4- (N-ethyl-N-phenylamino)-1,2 dimethyl-6-(methylamino)pyrimidinium chloride (ZD7288) (Sigma-Aldrich), or 1 µM TTX ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... mice were fasted for 6 h and received an oral (2 g/kg body weight) glucose load (G7021, Sigma Aldrich). For the insulin tolerance test ...
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bioRxiv - Systems Biology 2024Quote: ... About 75% of confluent cells were harvested and rinsed with 6 ml Dulbecco’s Phosphate-buffered Saline (DBPS) (Sigma Aldrich, USA). The cells were then trypsinized with 2 ml of 1 X trypsin and were incubated for 45 s (HEK 293 ...
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bioRxiv - Microbiology 2023Quote: ... or CEM were infected overnight in a 6-well plate in the presence of 0.5 μg/mL polybrene (Sigma-Aldrich). The following day ...
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bioRxiv - Molecular Biology 2023Quote: The resulting oxidized DNA samples (∼300ng) were mixed with 20 mM phosphate buffer (pH 6) and 5.1 mM (+)-biotinamidohexanoic acid hydrazide (Sigma-Aldrich), and incubated at 40°C for 4 hours ...
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bioRxiv - Neuroscience 2023Quote: ... and the AMPA receptor inhibitor 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 µM, Sigma Aldrich, St Louis, MO, USA). This solution was applied to the autaptic culture neurons for 2 min ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was eluted from the resin by applying 6 mL of wash buffer supplemented with 20 mM reduced glutathione (Sigma).
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bioRxiv - Biochemistry 2023Quote: ... Eluted and purified mGlu5-5M receptor and mGlu5-5M_W785A receptor mutant bound to quisqualate and PAM were concentrated to 5-6 mg/ml using Amicon Ultra-4 centrifugal concentrator 100 kDa (Millipore) for cryoEM grid preparation and analysis.
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bioRxiv - Cancer Biology 2023Quote: ... TMT-labeled samples were incubated in IP buffer consisting of 1% Nonidet P-40 with protein G agarose beads conjugated to 24 μg of 4G10 V312 IgG and 6 μg of PT-66 (P3300, Sigma) overnight at 4℃ ...
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bioRxiv - Biophysics 2023Quote: ... 1.5 – 2 million cells were plated on a 6-well-plate coated with 10 µg/ml fibronectin (Sigma; F1141-5MG) and maintained in IMDM (GlutaMAX ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting submucosa was cut into 6 inch pieces and decellularized using 4% alcohol/0.1% peracetic acid (v/v, Sigma-Aldrich) and washed thoroughly with 1X PBS and Type 1 water followed by lyophilization and comminuted into an injectable particulate form via cryogenic milling and sieving through 425 µM pore sieve ...
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bioRxiv - Cancer Biology 2023Quote: ... 6-week-old ElaK mice were first treated with tamoxifen (T5648-1G, Sigma Aldrich. 30 mg/ml in corn oil) by oral gavage and 4-hydroxytamoxifen (H7904-25mg ...
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bioRxiv - Cancer Biology 2023Quote: Cells were seeded in 6 cm dishes one day prior to treatment with 1 μg/ml actinomycin D (Sigma-Aldrich) for the indicated time ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in staining media supplemented with 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from analyses ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged at a 1:3 to 1:6 ratio every 7 days using cell dissociation solution-non enzymatic (Sigma) and plated in fresh BME matrix droplets.
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bioRxiv - Cell Biology 2023Quote: ... Hsp60 (1:1000, QED-BIOSCIENCE 11101) or glyceraldehyde-6-phosphate dehydrogenase (rabbit polyclonal anti-G6PDH antibody, 1:10000, Sigma A9521). Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000 ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then washed with 0.1% Tris-buffered saline and Tween-20 (TBST) prior to 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (D9542 Sigma) for nuclei visualization ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and then seeded in 6-well plates (5×105 cells/well) that had been pre-coated the day before with type I collagen (Sigma) dissolved in 0.02 N acetic acid ...
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bioRxiv - Cancer Biology 2023Quote: ... dissociated to single cell suspensions and cultured in RPMI-1640 (complete medium) supplemented with 60 μM 6-Thioguanine (Sigma-Aldrich) as previously described (18 ...
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bioRxiv - Cancer Biology 2023Quote: Bone marrow cells were acquired by flushing the femurs and tibias of 6- to 8-week-old female BALB/c mice and treated with red blood cell lysis buffer (Sigma). Lineage negative hematopoietic stem and progenitor cells (HSPCs ...
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bioRxiv - Physiology 2024Quote: ... followed by rehydration with different % of ethanol (From 100 % to 70 %, 111000190, Pharmco) and the antigen retrieval step with citric buffer (pH 6, C9999, Sigma) in heat for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... Strainers containing fRSS-washed cells were further washed using 500 µL of freshly prepared 6% sodium hypochlorite (Reagent Grade, Sigma) to remove SAB ...
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bioRxiv - Systems Biology 2024Quote: ... Reporter cell lines (100-150 million cells) were transduced with packaged retrovirus in the presence of 6 µg/ml polybrene (Sigma) and at low transduction efficiency (< 20% ...
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bioRxiv - Systems Biology 2024Quote: ... seven-week-old female C57Bl/6 mice were treated with oral administration of CCl4 (Sigma-Aldrich, St. Louis, MO, USA) diluted in olive oil ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the NPSH levels in the samples (50 µg of protein) were mixture of 50 µL of 6% TCA (Sigma Aldrich). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Expanded colonies were tested by inducing ER stress with 200 nM thapsigargin for 6 h (Sigma-Aldrich, Burlington, MA, USA) using confocal microscopy (Zeiss LSM800 controlled by the ZEN 2.3 software ...
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bioRxiv - Cell Biology 2024Quote: ... the respective secondary antibodies were incubated in ADB at room temperature in the dark for 90 min together with 0.5 µg/mL 4′,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich). Before imaging the cells were washed again using PBS ...
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bioRxiv - Microbiology 2024Quote: ... Indole-3-carboxaldehyde (I3A, catalog # 129445) and 6-Formylindolo[3,2-b]carbazole (FICZ, catalog # SML1489) were purchased from Millipore Sigma and reconstituted in DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... samples were washed in 1X PBS and incubated with DAPI (4-6-diamidino-2-phenylindole, Sigma-Aldrich, 1 µg/mL) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The migrated neutrophils in the apical compartment were lysed with 0.5% Triton X-100 and neutrophil MPO activity was quantified using a colorimetric assay using citrate buffered 2,2’-azino-bis(3-ethylbenxothiazoline-6-sulfonic acid) diammonium salt (ABTS) solution (Sigma-Aldrich). After 10 min of incubation ...
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bioRxiv - Plant Biology 2024Quote: ... 6 ml of the suspension was equally distributed and loaded on top of four continuous Percoll (Sigma Aldrich, Heidelberg, Germany) gradients (containing 50% Percoll / 0.4 M sorbitol ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 30 minutes on ice with 6 nm (Jackson#711-195-152) or 5 nm (Sigma#G7277) diameter immunogold coupled secondary antibodies in DMEM-FBS-HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... were treated with 50 µL of a 1mg/ml Concanavalin A (prepared in 10 mM sodium phosphate buffer pH 6, 10 mM CaCl2 ,1 mM MnCl2, 0.01% NaN3, Catalog C2010, Sigma Aldrich) solution ...
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bioRxiv - Systems Biology 2024Quote: ... Routine cells passaging was carried out at a 1:6 ratio every 2 days using 1× Trypsin–EDTA solution (Sigma) at 37°C ...
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bioRxiv - Physiology 2024Quote: ... slides were incubated at room temperature for 6 hours in staining solution containing 15 mM citrate buffer pH 5.3 (Sigma Aldrich), 2.8 mM CuSO (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: LUHMES-derived DA neurons (day 6 of differentiation) were treated with a combination of antimycin A (25 µM, Sigma-Aldrich), and oligomycin (10µM ...
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bioRxiv - Biochemistry 2024Quote: A DNA consisting of 2 base pairs-Widom 601 sequence (145 base pairs)-2 base pairs was amplified by PCR using a 5’-/6-FAM forward primer (Sigma). The PCR products were pooled from four 96-well PCR plates (100 µL per well ...
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bioRxiv - Bioengineering 2024Quote: ... 10 mL of MSC supplement and 6 mL of LAlanyl-L-Glutamine (ATCC, PCS-500-040) and 0.2 mg/mL G418 (Sigma, G8168) were included as medium supplements ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then blocked in 6% donkey serum and immunolabeled for protein gene product 9.5 (PGP9.5, 1:500, Millipore-Sigma, SAB4503057-100UG) as a pan-neuronal marker or rabbit-IgG (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were plated in in 24-or 6-well plates previously coated with Poly-L-ornithin (0,1 mg/ml, Sigma-Aldrich) at a density of 250,000 cells/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were acclimated to an isolated behavior space for 1hr prior to gavage with 100µl of sterile carmine red dye (6% w/v) (Sigma, C1022) dissolved in 0.5% methylcellulose (Sigma ...
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bioRxiv - Immunology 2024Quote: ... Hyde) were cultured for 4 days at 37ºC in BSK-II media and complete with 6% rabbit serum (Millipore Sigma) at pH of 7.6 ...
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bioRxiv - Cell Biology 2024Quote: ... the cell layers were washed with 200 µL of 6 M Guadinium Hydrochloride (Gdn-HCl, Sigma, Cat no. G3272-25G), 5 mM EDTA (Fluka ...
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bioRxiv - Microbiology 2024Quote: ... the nanoparticles were washed by centrifugation at 20,000 g once and then re-dispersed in 2 mL of a 6 mM sodium citrate solution (Sigma-Aldrich). Next ...
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bioRxiv - Immunology 2024Quote: ... each microfluidic device was filled with 60 µL of a hydrogel formulation containing the following components: 6 µL of 10x phosphate-buffered saline (PBS) (#5493, Sigma), 6 µL of 0.1 M sodium hydroxide (NaOH ...
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bioRxiv - Molecular Biology 2024Quote: ... HRS/Gsc-GFP dual reporter cells at day 6 of the ADE differentiation were isolated by washing the cells twice with PBS (Sigma; suitable for cell culture ...
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bioRxiv - Immunology 2024Quote: ... the slices were cultured for 4-6 days onto culture membranes (uncoated 30 mm Millicell-CM tissue culture inserts with 0.4 mm pores, Merck Millipore, Germany) and kept in six-well dishes (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... After 23 h, cells were fixed with 4% paraformaldehyde and stained with DAPI (4’, 6-diamidino-2-phenylindole) (Sigma-Aldrich, USA). Nine fields per well were imaged on an Operetta high-content imaging system (PerkinElmer) ...
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bioRxiv - Cancer Biology 2021Quote: ... Mia PaCa-2 cells and PCAFs were then transduced with the respective viral suspension at different multiplicity of infection (MOI) in the presence of 6 µg mL-1 hexadimethrine bromide (polybrene, Sigma H9268). Cells were sorted for mCherry and EGFP fluorescence using a BD FACSAria™ Cell Sorter (BD Biosciences ...