Labshake search
Citations for Millipore Sigma :
2301 - 2350 of 3014 citations for 6 bromochromone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: Total splenocytes were isolated from C57BL/6 and mFICD-/- mouse spleens and then treated for 3 days with 20 μg/ml LPS (Sigma), 100 µg/ml heparan sulfate or 2.5 μM thapsigargin (Enzo Life Sciences) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Trx-His(6)-Δlinker-hSLAP2 or Trx-His(6)-mSLAP were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
-
bioRxiv - Immunology 2020Quote: ... cells were resuspended in medium with 10% fetal calf serum and 1 × 105 cells were cultured for 4 to 6 days with different concentrations of ConA (Sigma) or co-cultured with (2.5 × 104 ...
-
bioRxiv - Immunology 2020Quote: ... grown at the log-phase in 6-well plates in the presence of DMEM supplemented with 1% FBS and 0.5% (w/v) methylcellulose (Sigma-Aldrich). After 2 d of incubation at 37°C ...
-
bioRxiv - Immunology 2020Quote: ... Eluate from amylose resin was concentrated and purified as a range of small oligomers on a Superose 6 size exclusion column (Cytiva) in lysis buffer, concentrated again (500 μL, A280 = 6) using a 50 kDa spin column (Millipore), and cleaved overnight with MBP-3C protease (50 μL ...
-
bioRxiv - Cell Biology 2020Quote: ... were seeded at 200,000 cells/well of 6 well plates and transduced with lentiviral particles at final MOI of 0.5 with 8 µg/ml polybrene (Sigma-Aldrich). Transduced cells were selected in 10 µg/ml puromycin (Gibco ...
-
bioRxiv - Immunology 2021Quote: ... or 0.5 μM/ml 5-amino-6-D-ribitylaminouracil (5-A-RU) (courtesy of Jeffrey Aubé, UNC) and 50 μM/ml Methylglyoxal (Sigma). Brefeldin A Solution (Biolegend ...
-
bioRxiv - Neuroscience 2021Quote: ... a pool of 4 brains was used (n = 6) and mixed with 600 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich). The homogenate was centrifuged at 2400 g for 10 min at 4 °C and the supernatants were collected for the analyses of the following parameters ...
-
bioRxiv - Microbiology 2022Quote: ... then resuspended in complete DMEM for analysis by flow cytometry on a Guava EasyCyte 6/2L bench cytometer equipped with 488nm and 532nm lasers (Millipore). Control wells were prepared without sporozoites to measure the basal level of dextran uptake ...
-
The intestinal circadian clock drives microbial rhythmicity to maintain gastrointestinal homeostasisbioRxiv - Physiology 2022Quote: Complete GI transit time (GITT) was measured by administering natural carmine red (6%, SigmaAldrich) dissolved in 0.5% methylcellulose (Sigma-Aldrich) by gavage (100ul ...
-
bioRxiv - Cancer Biology 2022Quote: ... see Figure S1A and 22) added to the cells with 400 µg/ml of poloxamer-407 (Sigma, 9003-11-6) and incubated in a 37°C CO2 incubator for another 24 hours (Figure 1A) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2.5×105 B16-F10 cells were plated into a 6-well dish and stimulated with growth media supplemented with 10 or 20 μM forskolin (Sigma) and 100 μM 3-isobutyl-1-methylxanthine (IBMX ...
-
bioRxiv - Biochemistry 2022Quote: ... The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626), 0.4 mg/L biotin and 0.5% methanol ...
-
bioRxiv - Biochemistry 2022Quote: ... with 3% BSA and incubated with the 3% BSA-blocked blotted membrane at 4 °C overnight in the absence or presence (as a negative control) of 10 mM free mannose-6-phosphate (M6P) (Sigma). The membranes were then washed three times with TBST (1×15 min and 2x 5min washes ...
-
bioRxiv - Biochemistry 2022Quote: ... a total of 0.4 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Sigma) and cells were fixed 4 days later ...
-
bioRxiv - Cancer Biology 2022Quote: ... Lentiviral transductions were performed in a 6-well plate format (3 × 105 cells/well) using 10 µg/mL polybrene (Merck Millipore). Stably transduced cells were flow-sorted.
-
bioRxiv - Developmental Biology 2022Quote: ... A few hours later the cells were infected with titer-adjusted volumes of each viral supernatant supplemented with 6 μg/ml (final concentration) of protamine sulfate (Sigma). After two days ...
-
bioRxiv - Cell Biology 2022Quote: NOD/SCID mice (6 weeks-old) were treated with an intraperitoneal (ip) injection of 650 mg APAP/kg (Sigma-Aldrich) to induce ALF ...
-
bioRxiv - Developmental Biology 2022Quote: ... 2/3 mTeSR1 + 1/3 N2 medium + LSX + PSD Day 6,7: 1/3 mTeSR1 + 2/3 N2 medium + PSD At day 6 cells were detached by Accutase solution (Sigma-Aldrich) and incubated at 37°C for 20 minutes to obtain a single cell suspension ...
-
bioRxiv - Cell Biology 2022Quote: The following monoclonal antibodies were used for immunofluorescence: anti-acetylated tubulin (mouse; clone 6-11B-1; Sigma-Aldrich; 1:500), anti-ubiquitin clone FK2 (mouse ...
-
bioRxiv - Cell Biology 2022Quote: ... The following incubations ensued at room temperature: 3×10 min in 0.1 M maleic acid buffer (Sigma-Aldrich; pH 6), 2 h in the dark in 0.5% (w/v ...
-
bioRxiv - Developmental Biology 2022Quote: ... The explants were cultured for 6 days in DMEM with 10% fetal bovine serum and 10 μg/ml ciprofloxacin (17850, Sigma), and then fixed in 4% PFA for 15min at room temperature before being processed for immunolabeling.
-
bioRxiv - Immunology 2022Quote: ... The sections were then washed with PBS/0.5% Triton and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Digitally scanned sections were analysed using Image Scope software (Scanscope XT ...
-
bioRxiv - Immunology 2022Quote: ... Lysates were assayed for phospho proteins using the Milliplex Cell Signaling kit (NFκB Magnetic Bead Signaling 6-plex, Millipore Sigma) for detection of NFκB pSer536 ...
-
bioRxiv - Immunology 2022Quote: ... Progenitors were spinfected in 6-well plates at 23-32°C for 30 minutes at 1000 x g with 4 µg/mL polybrene (Sigma) and 1 µM β-estradiol at 0.25 × 106 cells/mL for single gene knockouts ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... A volume of 40 µL of the mix (corresponding to 8.105 cells) was then plated on 6 cm Petri dishes filled with 2 mL of 2% Phytagel (Sigma-Aldrich), following Dubravcic et al (Dubravcic et al. ...
-
bioRxiv - Immunology 2022Quote: ... synthesized as previously described),6 a 5 mg/mL stock solution of PBI1 dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich) was prepared ...
-
bioRxiv - Plant Biology 2022Quote: ... total glutathione in 0.1 ml of the supernatant was measured by spectrophotometry in a 1 ml mixture containing 6 mM DTNB (Sigma-Aldrich), 3 mM NADPH ...
-
bioRxiv - Immunology 2022Quote: Dorsal and ventral side ears of either Mas-TRECK mice or wild type (C57BL/6) mice (WT) were injected intradermally (i.d.) with 10 ng of diphtheria toxin (DT) (Sigma-Aldrich) in 20 µl of PBS for one time ...
-
bioRxiv - Molecular Biology 2022Quote: ... the cells were washed several times in PBS and incubated with 1 µg/mL of 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) to visualize the nuclei.
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Chromosomes were stained with 4′,6-diamidino-2-phenylindole at a concentration of 5 μg/ml (DAPI; Sigma-Aldrich, D8417).
-
bioRxiv - Neuroscience 2022Quote: ... using 0.5 mM EDTA and either re-seeded with split ratios (1:3 to 1:8) onto 6-well plates or cryopreserved using CryoStor CS10 Cryopreservation Reagent (Sigma). iPSC lines were maintained in 5% CO2 ...
-
bioRxiv - Plant Biology 2023Quote: ... the embryos were arranged in glass Petri dishes (90 × 15 mm) and immersed in 6 mL stationary MS liquid culture medium (Murashige and Skoog, 1962) (Sigma) containing 0.1 g L-1 myo- inositol (Sigma) ...
-
Apicosome: newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cellsbioRxiv - Neuroscience 2022Quote: ... Cochlear cultures were treated for 6 h with various reagents (all dissolved in culture medium): 0.1% (v/v) DMSO (276855; Sigma-Aldrich), 0.5 mM BAPTA-AM (A1076 ...
-
bioRxiv - Neuroscience 2022Quote: ... The silk solution concentration was adjusted to 6 mg/mL and combined in a 10 cm dish with 400 to 500 µm sodium chloride (Sigma) particles at a ratio of 1:2 (v/w ...
-
bioRxiv - Neuroscience 2022Quote: ... mito-tempol[23] (25 μg, Sanbio) and oligodeoxynucleotide (3 μg/μl day 4, 5 and 6 after carrageenan, Sigma-Aldrich), were performed under light isoflurane anesthesia as described.[8a ...
-
bioRxiv - Molecular Biology 2022Quote: ... 500 mM NaCl and 6 mM β-mercaptoethanol) containing 1 mM phenylmethylsulfonyl fluoride (PMSF) and Protease inhibitor cocktail (Sigma-Aldrich) (20 mg/g of cell pellet) ...
-
bioRxiv - Cell Biology 2022Quote: ... Parasitemia was adjusted to 0.2-0.5% and 2% hematocrit and the culture was distributed in triplicates in 6-wells plate with various treatments: 400pM of the mimic (HMI0513-5NMOL, Sigma) or 400pM of the inhibitor (cat# 4464084 ...
-
bioRxiv - Bioengineering 2022Quote: ... PAAm matrices were activated twice with 0.5 mg/mL Sulfo-SANPAH for 6 minutes at room temperature (Sigma-Aldrich, 80332) and coated with 5 μg/mL fibronectin (Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... T cell subsets were rested overnight at 37°C then stimulated for 6 h with phorbol 12-myristate 13-acetate (25 ng/mL; Sigma) and ionomycin (1 mg/mL ...
-
Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... in PBS for 2 h at room temperature and counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; Sigma-Aldrich) to identify cell nuclei.
-
bioRxiv - Systems Biology 2024Quote: ... About 75% of confluent cells were harvested and rinsed with 6 ml Dulbecco’s Phosphate-buffered Saline (DBPS) (Sigma Aldrich, USA). The cells were then trypsinized with 2 ml of 1 X trypsin and were incubated for 45 s (HEK 293 ...
-
bioRxiv - Systems Biology 2024Quote: ... Reporter cell lines (100-150 million cells) were transduced with packaged retrovirus in the presence of 6 µg/ml polybrene (Sigma) and at low transduction efficiency (< 20% ...
-
bioRxiv - Systems Biology 2024Quote: ... seven-week-old female C57Bl/6 mice were treated with oral administration of CCl4 (Sigma-Aldrich, St. Louis, MO, USA) diluted in olive oil ...
-
bioRxiv - Physiology 2024Quote: ... followed by rehydration with different % of ethanol (From 100 % to 70 %, 111000190, Pharmco) and the antigen retrieval step with citric buffer (pH 6, C9999, Sigma) in heat for 20 min ...
-
bioRxiv - Microbiology 2024Quote: ... Strainers containing fRSS-washed cells were further washed using 500 µL of freshly prepared 6% sodium hypochlorite (Reagent Grade, Sigma) to remove SAB ...
-
bioRxiv - Neuroscience 2024Quote: Pharmacological experiments were carried out on PCs during simultaneous somatic and dendritic recordings after 10 minutes of control recording using ACSF with the following drugs: 20 µM 4- (N-ethyl-N-phenylamino)-1,2 dimethyl-6-(methylamino)pyrimidinium chloride (ZD7288) (Sigma-Aldrich), or 1 µM TTX ...
-
p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... mice were fasted for 6 h and received an oral (2 g/kg body weight) glucose load (G7021, Sigma Aldrich). For the insulin tolerance test ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and then seeded in 6-well plates (5×105 cells/well) that had been pre-coated the day before with type I collagen (Sigma) dissolved in 0.02 N acetic acid ...
-
bioRxiv - Microbiology 2024Quote: ... The pesticides were: 3,6-dichloropyridine-2-carboxylic acid (i e. clopyralid, pesticide analytical standard, CAS 1702-17-6, supplier: Sigma-Aldrich, purity ≥ 98.0% ...