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Citations for Millipore Sigma :
2351 - 2400 of 3014 citations for 6 bromochromone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Full length IMP2 protein was expressed as a pET-45 6-Histidine tagged 3C protease cleavable protein in Rosetta (DE3) pLysS Escherichia coli (Novagen) cells at 18 °C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... Excess biotin and DTT was removed by 6 x sequential dilution and concentration using 3KDa Amicon Ultra centrifugal filter (Millipore). Yield of biotin conjugation was tested by intact MS with an Agilent 6230 b LC/MS TOF mass spectrometer ...
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bioRxiv - Neuroscience 2024Quote: ... dissociated with forceps and digested at 37°C for 30 minutes via the addition of digestion solution (6 units of Papain (Sigma) and 9 units of DNase I (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK FRT cells were plated in 10-cm dish with culture medium containing only blasticidin S following co-transfection with Flp recombinase expressed plasmid pPGKFLPobpA and tetracycline-responsive plasmid pcDNA5FRT/TO containing TDP-43 Q331K or pcDNA5FRT/TO only as isogenic sham control in the ratio 6:4 using PEI (polyethyleneimine; Sigma) transfection method ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant of each sample was collected and acidified by adding up to 6 % trifluoroacetic acid (TFA) (Pierce) and 50% MS-grade acetonitrile (ACN) (Sigma). Precipitated lipids were removed by centrifugation at 14,000 RPM for 20 min ...
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bioRxiv - Microbiology 2024Quote: DDX5 KO HCT116 cells were transduced in 6-well plates using the lentiviral supernatant diluted in fresh medium supplemented with 4μg/mL of polybrene (Merck, Sigma-Aldrich). The medium was changed after 8 h and the selection with the appropriate antibiotic was initiated after 48 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged at a 1:3 to 1:6 ratio every 7 days using cell dissociation solution-non enzymatic (Sigma) and plated in fresh BME matrix droplets.
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bioRxiv - Cell Biology 2023Quote: ... Hsp60 (1:1000, QED-BIOSCIENCE 11101) or glyceraldehyde-6-phosphate dehydrogenase (rabbit polyclonal anti-G6PDH antibody, 1:10000, Sigma A9521). Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000 ...
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bioRxiv - Zoology 2024Quote: ... This washing step was followed by decalcification in 20X sample volume of pre-chilled 6% formic acid (Sigma-Aldrich, MO) for 24 h on the orbital shaker set to 55 RPM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... i.p.) young adult (4-week-old) C57BL/6 male mice and transferred to ice-cold DMEM/F12 media (Sigma Aldrich) in 6 well plates ...
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bioRxiv - Neuroscience 2023Quote: ... a total volume of 8.0 μL of freshly prepared 6-hydroxydopamine hydrobromide solution was given (Sigma-Aldrich, St. Louis, MO); 1.875 and 1.5 μg per μL in 0.9 % sterile saline with 0.02% ascorbic acid to deliver in two injection sites in the DLS 7.5 μg/injection for the restoration and voltammetry studies ...
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bioRxiv - Plant Biology 2024Quote: ... meliloti 1021 (pXLGD4) strain were stained using 5-bromo-6-chloro-3-indolyl-β-d-galactopyranoside (Magenta-Gal, Sigma-Aldrich), according to the protocol described previously by Jarzyniak et al ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3- to 6-hour-old eggs were collected and placed on fresh standard medium containing 0.25 mg/mL tetracycline hypochloride (Sigma-Aldrich), for all Drosophila strains ...
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bioRxiv - Genetics 2024Quote: ... 5 μg of dsRNAs were transfected into BmN4 cells (6 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Sigma). dsRNAs were repeatedly transfected every 3 days for four times ...
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bioRxiv - Evolutionary Biology 2024Quote: ... HEK-293T cells were cultured in 25 cm2 vented flasks at 37°C in a humidified 5% CO2 incubator in 6 mL of DMEM supplemented with 10% FBS and 0.5 mL of penicillin/streptomycin (Sigma-Aldrich, Canada ...
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bioRxiv - Immunology 2024Quote: ... slides were washed in PBS and incubated in 5 μg mL-1 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... the 3D collagen matrices were stained with 50 µM 5-(6)-carboxytetramethylrhodamine succinimidyl ester (TAMRA-SE; Sigma-Aldrich, Darmstadt, Germany) overnight at room temperature and subsequently rinsed with phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2024Quote: Cell supernatant fluid was concentrated using an Amicon Ultra-15 centrifugal filter from 6 mL to 500 µL at 4000 g during 30 min (100k, Merk Millipore). EVs were isolated using the IZON qEV original 35 nm size exclusion column (Izon science ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then washed with 0.1% Tris-buffered saline and Tween-20 (TBST) prior to 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (D9542 Sigma) for nuclei visualization ...
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bioRxiv - Immunology 2022Quote: ... were administered to C57BL/6 mice by subcutaneous (SC) injections on days 0 and 28 in the presence of Sigma adjuvant system (Sigma). 2 µg of S and S-EABR mRNA-LNP were administered to BALB/c mice by intramuscular (IM ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were plated at a density of 8x10^6 cells per 10 cm dish coated with 60 μg/ml poly-D-lysine (Sigma) and 2.5 μg/ml laminin (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... Disposable PS plastic was readily available as the flat bottom of a 6-well tissue culture plate (TPP®, Sigma). Disposable glass cover slides (22CIR-1 ...
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bioRxiv - Biochemistry 2022Quote: ... cells were mounted in Sigma-Aldrich DuoLink in situ mounting medium with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich), allowed to incubate at room temperature (RT ...
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bioRxiv - Cancer Biology 2023Quote: ... 3533-005-02) and overlaid with Organoid Media (Supplementary file 1) in 6-well ultra-low adhesion plates (Sigma, CLS3471). H2B:GFP retrovirus (91 ...
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bioRxiv - Immunology 2023Quote: ... Interphase cells were collected and incubated at 37°C in 5% CO2 for 6 hours with 20ng/ml Phorbol 12-Myristate 13-Acetate (PMA, Sigma), 1µg/ml Ionomycon (Sigma ...
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bioRxiv - Synthetic Biology 2023Quote: ... All samples diluted with buffer to the final concentration of 2 μM Aβ were supplemented with 6 μM ThT (Sigma), with a range of concentrations of the binders per experiment ...
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bioRxiv - Biochemistry 2022Quote: ... The protein samples of the peak fractions were collected and concentrated to 6 mg/ml using a 100 kDa molecular weight cut-off concentrator (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... centrifuged and the cell pellet was lysed using a Lysis Buffer (25 mM Tris pH 7.6, 150 mM NaCl, 1% NP-40, 1% Na-deoxycholate, and 0/1% SDS in water + protease inhibitor cocktail [Sigma] + phosphatase inhibitor cocktail [Sigma-Aldrich] + okadaic acid + sodium fluoride) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed three times with ice-cold PBS and incubated with 0.25 µg/mL biotin hydrazide (66640-86-6, Sigma-Aldrich) in cold PBS for 1 hour on ice ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of 3 µl of between 3-6 mg/ml of Toll5A-Spz1C gel-filtered complex mixed with 8 mM CHAPSO (final concentration, Sigma) were applied to the grids ...
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bioRxiv - Genomics 2023Quote: ... or cell type (roughly 5X106 iPSC-NPCs or 1X106 iPSC-neurons on a 6-well plate, enzymatically detached with Accutase (Millipore) and pelleted with centrifugation for 5 mins at 1,000 G) ...
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bioRxiv - Microbiology 2023Quote: ... quenched with 25 mM ammonium chloride in PBS for 10 min and nuclei were stained with 4’,6-diamidino-2-phenylindol (DAPI, Sigma, cat ...
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bioRxiv - Neuroscience 2023Quote: ... HEK cells were washed three times with ice-cold PBSx1 and lysed using Maltoside lysis buffer [1% N-dodecyl maltoside (cat no. 69227-93-6 – Sigma) dissolved in PBSx1 with freshly added 0.1 mg/ml PMSF and protease inhibitors] ...
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bioRxiv - Neuroscience 2023Quote: ... Adult female SNSiDTR (n=6) and TrkBiDTR (n=19) mice were injected i.p with 40 μg/kg DTX (Sigma, D0564) with a second dose after 72 h ...
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bioRxiv - Bioengineering 2023Quote: ... 3D collagen matrix systems were stained with 50 µM of 5-(and-6)-carboxytetramethylrhodamine succinimidyl ester (TAMRA-SE, Sigma-Aldrich) at room temperature for 60 minutes and visualized using a confocal laser scanning microscope (cLSM ...
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Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... 100 mM KCl and pre-loaded with 0.4 mM substrate (Gdm+ or guanylurea) and 1mM pyranine (trisodium 8-hydroxypyrene-1, 3, 6-trisulfonate; Sigma-Aldrich) using three freeze/thaw cycles ...
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bioRxiv - Microbiology 2023Quote: ... a dilution series of 107 to 103 cells/ml were plated in 5 μl spots on M9 minimal media plates with 10 mM glucose or 10 mM glucose-6-phosphate (Sigma) and antibiotics ...
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bioRxiv - Neuroscience 2023Quote: ... Mice fasted overnight were gavage fed 6% carmine red dye (300 µl) Sigma-Aldrich) in distilled water containing 0.5% methylcellulose (Sigma-Aldrich) and placed in individual cages with white paper covering cage bottoms ...
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bioRxiv - Immunology 2023Quote: ... 0.4 × 106 CXCR3A-transfected CHO cells/ml or 60 000 HMVEC/ml (2ml/well) were seeded in flat bottom 6-well plates (2.0 ml/well; TPP, Sigma-Aldrich) in supplemented Ham’s F-12 growth medium + 10% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 30min at room temperature (10µg/mL, D9542, Sigma-Aldrich). Coverslips were mounted with ProLong Gold Antifade (P36934 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... slides were washed for 5 min in 0.1x SSC with 1% Triton X-100 at 62°C and counterstained with 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich) in antifade with DABCO (1,4-diazabicyclo (2.2.2)-octane ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were also washed for 10 minutes in a solution of 1.25×10−3 mg/mL of 4′,6-diamidino-2-phenylindole (DAPI; Sigma: D9542) in PBS to fluorescently label nuclei within tissue sections.
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bioRxiv - Immunology 2023Quote: 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) was used to exclude dead cells and debris (Sigma Aldrich, St. Louis, MO). All antibodies are from Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... 1×106 cells were plated on one well of 6 well-plate previously coated with 10μg/ml of laminin (Sigma, #L2020) in 1xPBS for 1 hour at 37C ...
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bioRxiv - Genetics 2023Quote: ... Pellets of ∼6 x 108 cells were used to extract genomic DNA using acid-washed beads (Sigma-Aldrich, G8772-100G) and phenol-chloroform (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... dissected animal caps from injected and uninjected sibling embryos were cultured in 1X Steinberg’s solution for 6 hours 53 in the presence of 10 ng/ml activin (Sigma, A4941) and 1X 10-4 M retinoic acid (RA ...
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bioRxiv - Cell Biology 2023Quote: ... Gene mutation was induced 4 days after organoid passage during 6 days with 400 ng/ml doxycycline (D9891, Sigma-Aldrich). When indicated ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon tissues from one wild type and one Olfr78-GFP KO mice (respectively 8 and 6 months-old) were cut in small pieces and digested with 0.5 mg/ml Collagenase I (Sigma Aldrich) for 25 min at 37°C under agitation at 75 rpm ...